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Citations for Bio-Rad :
4651 - 4700 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Lysates were heated at 95 °C for 5 min together with Laemmli sample buffer (Bio-Rad, catalog #1610747). Lysates were resolved using a 4% to 15% Criterion TGX Stain-Free Precast gel (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... folic acid 500 μg/L) or on Columbia agar solid support enriched with 5% horse blood (COH) (Biorad). Anoxic conditions were generated in Gaspack (BD ...
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bioRxiv - Cell Biology 2023Quote: ... according to the manufacturer’s instructions and an iQ™5 real-time PCR System (Bio-Rad Laboratories, USA). All reactions were pre-denaturation at 95°C for 3 min ...
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bioRxiv - Developmental Biology 2023Quote: ... for 5 minutes each and then blocked for one hour in 0.5% Blotting-Grade Blocker (Bio-Rad, 1706404) diluted in TBST at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Real-time quantitative PCR was performed using the MyiQ™5 Real-Time PCR Detection System (Bio-Rad) using the following thermal cycle parameters ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was diluted 5-fold and qRT-PCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad) in CFX Connect Real-time System (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... 5 ng of cDNA was then subjected to qPCR analysis using iQ SYBR Green super-mix reagent (BioRad). Gene expression levels were normalized to a housekeeping gene (TATA-binding protein or HPRT) ...
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bioRxiv - Physiology 2024Quote: ... Protein (5-10 µg) was separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad, USA) in 26-well 4–15% Criterion™ TGX Stain-Free™ Protein Gels (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The formazan crystals formed by the cells were dissolved by adding 100 µL of 5% SDS (Bio-Rad) in 1N HCl (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... Integrity of the annealed oligonucleotides was determined by application to Mini-PROTEAN native 5% polyacrylamide TBE gel (Biorad), staining with SYBR safe DNA gel stain (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Pooled peak fractions were then loaded onto a 5 ml Bio-Scale Mini CHT Type I Cartridge (Biorad) in 10 mM NaH2PO4 Buffer and eluted sequentially with 2CV 40 mM NaH2PO4 Buffer ...
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bioRxiv - Genetics 2024Quote: ... Electroporation was performed at 2.5 kV for 5 ms in a MicroPulser electroporator (Bio-Rad, Hercules, California, USA) using 0.2 cm cuvettes ...
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bioRxiv - Cancer Biology 2024Quote: ... at 95 degrees Celsius for 5 minutes before loading onto 10% Mini-PROTEAN TGX precast protein gel (BioRad). PageRuler Plus Pre-stained Protein Ladder (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and 5 µg total protein was loaded on each lane of a Mini Protean TGX Gel (BioRad, #4561093) together with a standard (WesternC ...
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bioRxiv - Biochemistry 2024Quote: ... boiled for 5 min and then resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Immunology 2021Quote: ... Viral stocks were normalized by p24-Gag content as measured with an Enzyme Linked Immunosorbent Assay test (GenscreenTM HIV-1 Ag Assay; Bio-Rad, Marnes-la-Coquette, France). Virions were used to infect CEM.NKR-CCR5 cells after ELISA-p24 quantification and normalization ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were then transferred onto a nitrocellulose membrane (GE-Healthcare, Chicago, IL, USA) for 1 hour at 15V using the Trans-Blot semi-dry transfer system (Bio-Rad Laboratories, Hercules, CA, USA). After transfer ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... Both primary antibodies were diluted 1:5,000 in PBST with 0.2% milk and subsequently conjugated with α-rabbit antibody-HRP conjugate (BioRad Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate #1705046 ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and incubated overnight at 4°C in primary antibodies: anti-human actin-β primary monoclonal antibody produced in mouse (1:100; Bio-Rad Laboratories Inc. MCA57766A) and non-muscle myosin II produced in rabbit (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... pH 9.5). Quantification of Western blot images (SFig. 1-4) was carried out using ChemiDoc™ XRS+ Image Lab™ Software (Bio-Rad Laboratories AB, Sweden); statistical analysis was performed with GraphPad Prism software (version 5.0 ...
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bioRxiv - Neuroscience 2021Quote: ... was resuspended in 1 ml of PBS/Ca+/Mg+ buffer and protein content was quantified by DC protein assay kit (Bio-Rad Laboratory, Hercules, CA, USA). 500 µg of synaptosomes were incubated 60 min at 4 °C with sulfo-NHS-biotin (1.5 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: To prepare the tubing for gene gun delivery one tubing and 25 mg of Au particles with 1 µm Ø was used from Helios Gene Gun Optimization Kit (Bio-Rad, Hercules, CA, USA). 10 µg of the synthesized RNA transcript was used four labeling Au particles ...
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bioRxiv - Systems Biology 2022Quote: ... shortly centrifuged (2,000xg, 1 min) and the plate heated at 95°C for 60 min in a thermal cycler (Biorad’s S1000 with 384-well reaction module) at a constant lid temperature of 110°C ...
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bioRxiv - Bioengineering 2024Quote: ... gene and the HIV-1 Psi vector sequence were performed using respectively custom qPCR HEX assay and custom qPCR FAM assay (Bio-Rad, Marnes-la-Coquette, France) with previously published primer sequences.48 qPCR reactions were performed using 200 ng of gDNA and the Master Mix Taqman Universal II (Applied Biosystems ...
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bioRxiv - Developmental Biology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies used were goat anti-rabbit IgG or anti-mouse IgG (Bio-Rad, 170-6515 or 170-6516, 1:3,000). To prevent signal overlap from IgG heavy chain with eRF1 ...
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bioRxiv - Neuroscience 2022Quote: ... and protein standards were mixed with Laemmli buffer and loaded on 4-15% Criterion TGX Stain-Free protein gels in 1×□TGS running buffer (all from Bio-Rad, Marnes-la-Coquette, France) and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... The transfer “sandwich” was exposed to 25 V and 1 A for 30 mins by a Trans-Blot TURBO transfer system (Bio-Rad, catalog no. 170-4155). The membrane was incubated in 4.0% dry milk in PBS buffer ...
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bioRxiv - Neuroscience 2022Quote: ... and then stained for 1 minute with 0.1% (w/v) Coomassie brilliant blue R-250 (CBBR, Cat#1610400, Bio-Rad Laboratories Inc., Hercules, CA, USA) in methanol/Milli-Q water (1:1) ...
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bioRxiv - Neuroscience 2022Quote: ... We sampled 1 µl of a cell suspension and quantified the number of living cells by a TC20 automated cell counter (Bio-Rad Laboratories, Hercules, CA USA). Finally ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Immunoblots were detected with an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (1:2000; NA934 or NA 931; Amersham BioSciences, Buckinghamshire, UK) by ECL detection (Bio-Rad Laboratories, Hercules, CA, USA). β-Actin served as a loading control.
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bioRxiv - Microbiology 2024Quote: ... Then, 3 mL of each culture were pelleted (11,000 rpm, 1 min) to perform total RNA extraction (using BioRad’s Aurum™ Total RNA Mini Kit). RNA concentration and purity were assessed using a nanodrop and all samples shown to have A260/A280 and A260/A230 ratios ≥ 2 ...
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bioRxiv - Immunology 2024Quote: Activity of caspase-1 was measured in macrophages after co-culture by incubating with FAM-YVAD-FMK FLICA (10 μM, Bio-Rad, as per manufacturer instructions) for 60 minutes with flow cytometry.
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bioRxiv - Immunology 2024Quote: Inflammatory cytokines and chemokines in sera were analyzed using a Bioplex Mouse cytokine 23-plex panel magnetic bead Luminex assay (Biorad, 23-plex, Group 1, #64436152) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then incubated overnight at 4°C with antibody cocktail consisting of rat anti-F4/80 (1:100) (catalog #: MCA497; Bio-Rad Laboratories; Hercules, CA, USA), rabbit anti-vimentin (1:100) ...
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bioRxiv - Genomics 2024Quote: ... Real-time PCR was performed in triplicate using the primers listed in Supplementary Table 1 and a CFX96 Real-time System (Bio-Rad Laboratories, Richmond, VA, USA). Expression levels were normalized to that of glyceraldehyde 3-phosphate dehydrogenase (GAPDH).
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bioRxiv - Molecular Biology 2020Quote: ... samples were boiled for 5 min and equal volumes were loaded onto a 4-15% gradient gel (Bio-Rad) for SDS-PAGE ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Each female was macerated in a 50 μl 5% Chelex (Chelex 100 Resin, Bio-Rad Laboratories, Hercules, CA, USA) solution and 1μl proteinase K ...
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bioRxiv - Developmental Biology 2021Quote: ... The PVDF membrane was blocked for 30 minutes at RT using 5% Blotting-Grade Blocker (170-6404, BIO-RAD)/Tris buffered saline with Tween 20 (TBST ...
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bioRxiv - Microbiology 2022Quote: ... NS5B-FAM probe: 5’-ATGGGTTCGCATGGTCCTAATGACACAC-3’) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 μL reaction contained 500 ng of total RNA ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μl of lysate was used for protein determination by the DC Protein Assay (Bio-Rad Laboratories, Hercules, CA).
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bioRxiv - Genomics 2022Quote: ... The gel was then soaked into the staining solution for 5 minutes and then directly scanned by Bio-rad ChemiDoc™ Touch Imaging System and the gel image was analyzed.
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bioRxiv - Developmental Biology 2020Quote: ... the membrane was blocked overnight at 4°C using 5% non-fat dry milk (Bio-Rad, catalog 170-6404) in Tris-buffered saline pH 7.3 ...
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bioRxiv - Microbiology 2020Quote: ... or by hand at 5 μl per well into 96-well hard shell low profile PCR plates (Bio-rad) for bulk RNA sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were heated at 55° C for 5 minutes and 30 µg of protein per sample was run on Tris-Tricine 16.5% precast polyacrylamide gels (BioRad) for Aβ or 10% Tris-Glycine (TGX ...
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bioRxiv - Neuroscience 2020Quote: ... 15 μg total RNA from HEK293 cells were separated on 5% Criterion™ TBE polyacrylamide gels (#3450048, Bio-Rad) and transferred to positively charged nylon membrane ...
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bioRxiv - Cancer Biology 2020Quote: ... Alkaline phosphatase was developed with 5-bromo-4-chloro-3-indolyl phosphate and Nitroblue tetrazolium (BCIP/NBT; BioRad, Germany) as substrate ...
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bioRxiv - Physiology 2021Quote: ... Samples (5 μL) were then loaded on precast gradients (4-15%) SDS-polyacrylamide gels in duplicate (Bio-Rad Laboratories) and subjected to electrophoresis at 180 V for 40 minutes using pre-made 1x SDS-PAGE running buffer (Ameresco) ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclear (5 µg total protein) and cytoplasmic (10 µg total protein) fractions were boiled in Laemmli buffer (Bio-Rad), run on Criterion 4-20% Gels (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... 40 µL of supernatant containing 5 µg of proteins were mixed to 36 μL Laemmli Buffer 1x (Bio-Rad) and 4 µL β-mercaptoethanol BioUltra (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... the membrane was placed in Pierce chemiluminescence substrates for 5 minutes and imaged on film using ChemiDoc (Bio-Rad).