Labshake search
Citations for Bio-Rad :
2101 - 2150 of 2175 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... Native protein extracts from E.coli or Synechococcus ΔCS were incubated in IMAC HEPES binding buffer supplemented with potential effector molecules and Ni-charged iminodiacetic acid Profinity™ IMAC Resin (Bio-Rad, USA) on a low speed rotator for 1 h at 23°C ...
-
bioRxiv - Biophysics 2021Quote: ... and 3.6 μL ddH2O in a 10 μL total reaction volume using Bio-Rad CFX Connect™ Real-Time PCR System (Bio-Rad, CA, USA). The 2-ΔΔCt method was used for the quantitative analysis ...
-
bioRxiv - Cell Biology 2020Quote: ... RNA template (1 μg) was prepared and reverse transcription reaction was performed with iScript Advanced cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA) according to the procedure described in the product’s instruction manual ...
-
bioRxiv - Microbiology 2020Quote: ... Droplets were generated to a 20 μL reaction volume using the automated droplet generation oil for probes (Bio-Rad Laboratories, Inc., Cat. No. 1864110) and the plate was sealed ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3 uL of a 1:20 dilution of the cDNA was used as input into each 10 uL qPCR reaction using the SsoAdvanced™ Universal SYBR® Green Supermix (BioRad; Cat #: 1725271). mex-6 ...
-
bioRxiv - Developmental Biology 2020Quote: ... cDNA was diluted 18-fold and 4 µL was used in a reaction for SYBR green detection with SsoAdvanced SYBR Green supermix (Bio-Rad, catalog no. 1725270) on a CFX384 machine (Bio-rad) ...
-
bioRxiv - Developmental Biology 2020Quote: ... cDNA was diluted 20-fold and 4 µL was used in a reaction for SYBR green detection with SsoAdvanced SYBR Green supermix (Bio-Rad, catalog no. 1725270) on a CFX384 machine (Bio-rad) ...
-
Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in developmentbioRxiv - Genomics 2020Quote: ... qRT-PCR reactions were performed in triplicate with 10 ng of template cDNA per reaction using a CFX96™ Real-Time PCR Detection System and the iQ™ SYBR® Green Supermix (Bio-Rad). PCR of the TATA binding protein (TBP ...
-
bioRxiv - Genomics 2020Quote: ... qRT-PCR reactions were performed in triplicate with 10 ng of template cDNA per reaction using a CFX96™ Real-Time PCR Detection System and the iQ™ SYBR® Green Supermix (Bio-Rad). PCR of the TATA binding protein (TBP ...
-
bioRxiv - Microbiology 2021Quote: The 20 μl RT-dPCR reaction mixture consisted of 5 μl One-Step RT-dPCR Advanced Supermix for Probes (Bio-Rad, Laboratories, Munich, Germany), 2 µl of Reverse Transcriptase (Bio-Rad ...
-
bioRxiv - Biochemistry 2020Quote: To visualize protein expression 1 μL of the TXTL reactions was mixed with 2 μL of water and 3 μL of 2x Laemmli loading dye (Bio-Rad, Hercules, CA, USA) and incubated at 90°C for 3 min ...
-
bioRxiv - Plant Biology 2020Quote: ... the cDNA was synthesised using the Bio-Rad iScript™ cDNA Synthesis Kit (25 x 20 µl reactions #1708890, Bio-Rad Laboratories Inc., USA), while the qPCR reaction was performed using the Applied Biosystems PowerUp™ SYBR™ Green Master Mix (A25741 ...
-
bioRxiv - Plant Biology 2020Quote: ... 0.02 µg of cDNA was used in a 20 µl reaction containing 10 µl of SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad, US), 6 µM of forward and reverse primers and 3 µl of RNAse-free water ...
-
bioRxiv - Microbiology 2021Quote: ... Reactions were performed in triplicate in a total volume of 20 μL that included 2 μL of the template or standard plasmid and 250 nM of each primer and TaqMan (5’ hydrolysis) probe (the RT-qPCR reactions also included 0.5 μL of Bio-Rad iScript advanced reverse transcriptase). Thermocycling conditions for qPCR consisted of an initial 50 °C step for 2 min followed by 95 °C for 10 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... 300nM final volume of each forward and reverse primer pair, 5 μL SYBR Green Supermix (50% total volume of reaction, rxn) (Bio-Rad Inc. Hercules, CA), and nuclease-free water (to make a final reaction volume of 10 μL) ...
-
bioRxiv - Physiology 2022Quote: ... qPCR reactions were carried out in duplicate with a final reaction volume of 20 μl using iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, USA). The following qPCR conditions ...
-
bioRxiv - Molecular Biology 2022Quote: ... Chemiluminescence reaction was initiated with Western Bright Quantum Western blotting detection kit and measured in a ChemiDocTM imaging system (BIO-RAD, Hercules, CA, USA).
-
bioRxiv - Bioengineering 2021Quote: ... incubated for 5 min at 95°C and used as template in the qPCR reaction in a final 10−4 dilution with the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA). The qPCR was run in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Developmental Biology 2020Quote: Three technical replicates of 10 ul qPCR reactions per sample were prepared using Bio-Rad SsoAdvanced universal SYBR green master mix (Bio-Rad, Hercules, CA 1725271) and were run on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad 1855195) ...
-
bioRxiv - Physiology 2020Quote: ... Amplification reactions and disassociation curves of 4-fold dilution series of cDNA were carried out on a CFX384 machine (Bio-Rad, Bio-Rad, Mississauga, ON), for determination of primer efficiencies ...
-
bioRxiv - Immunology 2020Quote: ... we added 100μl of filtered TMB substrate for 15 minutes and blocked the reaction with H2S04 before the read-out (OD measurement was done at 450nm on the Bio-Rad iMark microplate reader).
-
bioRxiv - Immunology 2020Quote: ... a real-time PCR reaction mixture was prepared containing 10 µL of SsoAdvanced™ SYBR® Green Supermix (Bio-Rad. Cat. No. 172-5265), 1 µL each of 5 µM gene-specific forward and reverse primer pair ...
-
bioRxiv - Evolutionary Biology 2021Quote: The ddPCR reactions and the initial PCR optimization experiments were performed on the QX100/200 Droplet Digital PCR System (Bio-Rad, Hercules, CA, USA). The copy numbers of all CNV genes in our study were estimated relative to a fragment of the Tert gene ...
-
bioRxiv - Neuroscience 2021Quote: Each sample was run in three technical replicates per gene in a 10 uL qPCR reaction using Bio-Rad SsoAdvanced universal SYBR green master mix (Bio-Rad, Hercules, CA 1725271). Samples were run on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad 1855195) ...
-
bioRxiv - Developmental Biology 2021Quote: cDNA samples were diluted to a working concentration of 5ng/uL and qRT-PCR reactions were performed with the SsoAdvanced SYBR Green Supermix (BIO-RAD Cat#172-5261) using a CFX96 Thermal Cycler (BIO-RAD ...
-
bioRxiv - Immunology 2021Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories, Hercules, CA) and the Taqman primers Cftr (Mm00445197_m1) ...
-
bioRxiv - Cell Biology 2022Quote: ... was performed using 5uL of the total cDNA (derived from 100ng RNA) per reaction with 20uL of cocktail containing 2x SYBR green supermix (Biorad, Hercules, California, United States), gene-specific Primer Pairs (IDT ...
-
bioRxiv - Microbiology 2023Quote: ... Droplets were generated using the Bio-Rad QX200™ Droplet Generator and DG8™ Cartridges with 20 μL of reaction mix and 70 μL of Droplet Generation Oil for Evagreen (Bio-Rad, CA, USA). Following droplet generation ...
-
bioRxiv - Molecular Biology 2022Quote: ... Reactions were performed in total 24ul volume using 12ul of 2 x ddPCR Supermix for Probes (No dUTP) (Bio-Rad Laboratories, catalog no. 1863024), 900nM of target primer pairs ...
-
bioRxiv - Microbiology 2024Quote: ... The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR) assays on a T100™ Thermal Cycler (Bio-Rad, Hercules, CA). Reaction volumes were 25 µL and consisted of 12.5 µL of Accustart II PCR Toughmix (Quantabio ...
-
bioRxiv - Genomics 2022Quote: ... 22 μL reactions were prepared with 11 μL of 2x ddPCR™ Supermix for Probes (No dUTP) (Bio-Rad Laboratories, Inc., Cat. No. 1863023), 0.4 μM of all probes and primers ...
-
Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... DNA was diluted to 10 ng/μl and 2 μl were used in each RT-qPCR reaction with 10 μl of iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA) and 400 nM of forward and reverse primers ...
-
bioRxiv - Cell Biology 2023Quote: ... were removed from the eluted samples post-purification and quantified using quantitative Real Time polymerase chain reaction using the Bio-Rad CFX 96™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA) and Promega Plexor®HY Quantitation System (Promega Corporation ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 3.6 μL of ddH2O in a 10 μL total reaction volume using Bio-Rad CFX Connect™ Real-Time PCR System (Bio-Rad, CA, USA). The expression of the housekeeping gene GADPH (XM393605 ...
-
bioRxiv - Biochemistry 2023Quote: ... 70 μl of the resulting reaction mixture was first passed through a Micro Bio-Spin Bio-Gel P-30 spin column (Bio-Rad; 40 kDa MWCO) equilibrated with 20 mM HEPES pH 7.9 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... real-time PCR was performed in a reaction volume of 20 μL comprising 10 μL Mastermix with SYBR green (Bio-Rad, Hercules, CA, USA), 0.6 μL each primer (1.2 μM) ...
-
bioRxiv - Immunology 2024Quote: ... cDNA was synthesized using 100 ng of purified total RNA in a 20 μL volume reaction with an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA), as described in (52) ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted at a concentration of 5 ng/µl and 2 µl were loaded in duplicate in a 96-well qPCR plate with the reaction mix comprising 5 µl of Sybr Green master mix (Bio-Rad, Hercules, CA, USA), 2 µl of water and 1 µl of primer mix containing forward and reverse primers for STAT3 and P53 ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNA samples were mixed with PerfeCTa® SYBR® Green FastMix® Reaction Mixes (QuantaBio, 95072) and were analyzed by a CFX 384 Real-Time system (Bio-Rad). All primers and sequences are listed in Table 1-1 and available upon request.
-
bioRxiv - Bioengineering 2023Quote: ... To confirm PEGylation the product and reactants were analyzed by SDS-PAGE using Mini-PROTEAN® TGX™ Precast protein gels (Bio-Rad Laboratories Inc., CA, USA).
-
bioRxiv - Molecular Biology 2021Quote: ... The bound crystal violet was eluted with 33% acetic acid (Mallinckrodt Chemicals, Phillipsburg, NJ, USA) and quantified by measuring the absorbance at 595 nm with a microplate reader (Bio-Rad, Hercules, CA, USA). The relative ability of migration was calculated as folds changed in absorbance of the indicated sample in relation to the control ...
-
bioRxiv - Bioengineering 2020Quote: ... quantification was performed using a Bio-Rad Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 65 °C ...
-
bioRxiv - Bioengineering 2020Quote: ... Chromatographic separation was performed using a Rezex Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 55 °C ...
-
bioRxiv - Microbiology 2021Quote: ... and lactate was achieved with a Bio-Rad Aminex® 87H Ion Exclusion Column for organic acids (Part No. 1250140, Bio-Rad, Hercules, CA) with a mobile phase of 5 mM sulfuric acid ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Chromatographic separation was performed using a Bio-Rad Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 65 °C ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Chromatographic separation was performed using a Bio-Rad Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 65 ℃ ...
-
bioRxiv - Microbiology 2021Quote: ... using a standard analytical system (Shimadzu, Kyoto, Japan) equipped with an Aminex Organic Acid Analysis column (Bio-Rad, HPX-87H 300 x 7.8 mm) heated at 60 °C ...
-
bioRxiv - Microbiology 2021Quote: ... using a standard analytical system (Shimadzu, Kyoto, Japan) equipped with an Aminex Organic Acid Analysis column (Bio-Rad, HPX-87H 300 x 7.8 mm) heated at 60° C ...
-
bioRxiv - Systems Biology 2022Quote: Samples from fermentation experiments were processed for glucose and organic acid measurement by HPLC with an Aminex HPX-87H column (Bio-Rad, Hercules CA, USA) at 45°C and 5 mM Sulfuric acid as mobile phase ...
-
bioRxiv - Cell Biology 2024Quote: ... Protein bands of interest were detected using a chemiluminescent reagent (luminol, p-coumaric acid, and hydrogen peroxide) and a ChemiDoc system (Bio-Rad, Hercules, CA, USA). Densitometric analysis was performed using ImageJ software v1.53 (National Institute of Health ...