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Citations for Bio-Rad :
1751 - 1800 of 2175 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The reacted proteins were detected by incubating the membranes in the chemiluminescence solution (luminol, coumaric acid, hydrogen peroxide, Tris pH 6.8) and imaged by the ChemiDoc Gel Imaging System (BioRad, USA).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... which binds non-selectively to nucleic acids and was imaged by the ChemiDoc Imaging System from Bio-Rad (California, USA). The bands were subsequently quantified using ImageJ software ...
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bioRxiv - Cell Biology 2020Quote: ... The product yield for each condition was estimated by resolving 10% of the reaction on a 1% EtBr-agarose gel and quantifying the band intensity of the resulting amplicon with ImageLab software (Bio-Rad). The 1.6-kb amplicon was gel-extracted using the QiaQuick Gel Extraction Kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... TMB substrate was added to stop the reaction before performing the reading at 450 nm in the ELISA plate reader (iMark Microplate Reader, Bio-Rad).
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.1 ng total cellular DNA and 0.25 μM sense and antisense primers were used in a 10 μl reaction with iTaq Universal SYBR Green Supermix (Bio-Rad). The reaction volumes were heated at 95 °C for 3 min ...
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bioRxiv - Genomics 2020Quote: ... and 5μl of each sample was added to each RT-qPCR reaction that were amplified using iTaq Universal SYBR Green Supermix from Bio-Rad and read on a StepOnePlus Real-Time PCR System from Applied Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: ... GEM-RT was performed in a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad; P/N 1851197): 53°C for 45 min ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was mixed with 5× RT Reaction Buffer and 10× RT Enzyme Mix and transferred to a thermal cycler (Bio-Rad). Cycling conditions were as follows ...
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bioRxiv - Genomics 2020Quote: ... q-PCR reaction was performed on Bio-Rad CFX RealTime PCR system using iTaq Universal SYBR Green Supermix (Bio-Rad, 1725125)
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bioRxiv - Immunology 2021Quote: ... 1/10 dilutions were used in triplicates with 0.2 μM primer and 10 μL LightCycler 480 SYBR Green I Master in a 20 μL reaction and qPCR executed in a 98- well block on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Absolute transcript levels for TDP-43 ...
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bioRxiv - Physiology 2020Quote: ... A total of 1000 ng of total RNA was used to prepare cDNA in a 20 µL reaction with Script Reverse Transcription Supermix (Bio-Rad). qPCR was performed on technical duplicates with cDNA diluted 30x and SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was performed using a CFX384 Touch Real-Time PCR Detection System (Bio-Rad). Quantification of mRNA expression was calculated by the DCT method with 18S ribosomal RNA as the reference gene.
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bioRxiv - Genetics 2021Quote: ... The cDNA was used as template for the droplet digital PCR (ddPCR) analysis as follows: ddPCR reaction mixture (12.5μL ddPCR Supermix for Probes (No dUTP) (Bio-Rad, Hercules, CA), 1 μL specific primers of WT and mutated alleles (Supplemental Table 3) ...
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bioRxiv - Genetics 2020Quote: Polymerase chain reactions (PCR) were carried out on an MJ Research DNA Engine Tetrad PTC-225 (Bio-Rad Laboratories, Watford, UK). Forward and reverse primers were designed according to previously published work (Cothran et al. ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative PCR (qPCR) reactions were performed in a final volume of 20 µl containing SsoFast™ EvaGreen® Supermix (Bio-Rad) using CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Partitioning of the reaction mixture into up to 20,000 droplets was carried out using a QX200 dPCR droplet generator (Bio-Rad, Laboratories) and PCR was performed on the Mastercycler Pro (Eppendorf ...
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bioRxiv - Cell Biology 2021Quote: ... qRT-PCR reactions were performed using iTaq Universal SYBR Green in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). The ΔCt method (2–ΔCt ...
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bioRxiv - Neuroscience 2021Quote: The qPCR reactions were performed using SsoAdvanced Universal SYBR Green Supermix on a CFX96 Touch Real-Time PCR Detection System (Bio-rad). Primer (Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Droplets were next transferred to a 96-well plate before to start the PCR reaction in thermal cycler (C1000 Touch Thermal Cycler, Bio-Rad) under the following conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... and indicated primers (2 μL) were added in the PCR reactions (ddPCR Supermix for Probes (No dUTP) mix from Bio-Rad) in a total of 25 μL ...
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bioRxiv - Cell Biology 2021Quote: 500 picogram of eluted DNA was used as a template for qPCR analysis in a 20µL reaction with the following primers on a BioRad CFX96 Touch Real-Time PCR Detection System (BioRad, cat. #1855196) using Power SYBR Green PCR master mix (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... 16S rDNA PCR reactions were used the MyiQ single-color real-time PCR detection system (Bio-Rad Laboratories, Hercules, CA, USA) and iTaqTM Universal SYBR green supermix (Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real-time polymerase chain reaction was carried out in a final volume of 20 μl with iTaq Universal SYBR Green Supermix (Bio-Rad) containing 2 μl of five times diluted cDNA and monitored by CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... Gene expression analysis were performed by quantitative RT-qPCR in a Viia7PCR device with 5 μl reaction mix containing SyberGreen Supermix (Bio-Rad). Tomato actin was used as a reference gene and fold change in expression were calculated based on 2^(-ddCt ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR was performed in a 10 μL reaction using iQ™ SYBR® Green Supermix (Bio-Rad, Hercules, CA, USA). Applied Biosystems 7300 Real Time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... reaction prepared using a SsoAdvanced Universal SYBR Green Supermix and cycled on a CFX96 Real-Time PCR Detection System (Bio-Rad), following the supplier’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 µg of each RNA sample was used in a 10-µL reverse transcription reaction set up using the iScript Select cDNA synthesis kit (Bio-Rad), together with gene-specific reverse primers (Table S2 ...
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bioRxiv - Plant Biology 2021Quote: ... ∼1 ug of RNA from resulting extraction was used as template in standard cDNA synthesis reaction (BioRad iScript cDNA Synthesis Kit). 200 ng of resulting cDNA was used in qRT-PCR reactions to quantify POL expression levels (PowerUp SYBR Green 5x Master Mix-Thermo) ...
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bioRxiv - Genetics 2020Quote: cDNA synthesis was performed with one μg of total RNA in 20 μl reactions using the iScript Reverse Transcription Supermix (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2021Quote: ... 0.4 μM or 3.2 μM of SAM-3H3 were used and the reaction was cleaned with either a PCR purification kit or Bio-Spin P30 columns (Bio-rad). The reactions were incubated for 2 hrs at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) of the RNA was performed using a CFX96 Real-Time PCR Detection System (Bio-Rad) using the KAPA SYBR FAST One-Step qRT-PCR kit (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... messenger RNA expression was analyzed by quantitative real-time polymerase chain reaction on a BioRad CFX machine using pre-validated primers with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). GAPDH gene expression was used as the reference gene and the specificity of the assay was confirmed by melting curve analysis.
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bioRxiv - Cancer Biology 2020Quote: ... Amplification reactions were performed in duplicate using 96 well-plates using a CFX96 Touch Real-time PCR Detection System (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... The cDNA was then employed for quantitative polymerase reaction (qPCR) using a SsoAdvanced™ SYBR® Green Supermix (Bio-Rad, USA) with denaturation at 95 °C for 30 s ...
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bioRxiv - Immunology 2022Quote: ... was utilized to measure the 16S concentration in each sample with the following reaction components: 1X QX200 EvaGreen Supermix (Bio-Rad), 500 nM forward primer ...
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bioRxiv - Cell Biology 2022Quote: ... 20 ng of cDNA and 0.4 μM of each primer set was mixed in a 10 μl RT-PCR reaction that was ran on the CFX96 System (Bio-Rad). The primers that were used span exons ...
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bioRxiv - Systems Biology 2022Quote: ... filtered supernatant from HGT experiments was added to a qPCR reaction mix containing 2X SsoAdvanced™ Universal Probes Supermix (Bio-Rad) and PrimeTime qPCR Assays (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... A volume of cDNA corresponding to 5 ng of the input RNA was subjected to quantitative PCR (qPCR) in a 10 μl reaction using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) and 0.4 μM of each forward and reverse primer ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR reactions were performed using THUNDERBIRD SYBR qPCR Mix (TOYOBO #QPS-201, Japan) and CFX Connect Real-Time System (Bio-Rad) with the following conditions ...
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Methacrylic acid-based biomaterials promote peripheral innervation in the subcutaneous space of micebioRxiv - Bioengineering 2022Quote: ... according to the manufacturer’s instructions and assayed for mRNA of interest using BioRad Ssofast Evagreen Reaction Mix (BioRad Laboratories, Mississauga, ON). Primers (Table 1 ...
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bioRxiv - Bioengineering 2022Quote: ... Quenched modification reactions were purified using the manufacturer’s procedure for desalting on BioRad Econo-Pac 10DG Desalting Columns (Biorad, Hercules, CA) to remove excess mTz modifier ...
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bioRxiv - Neuroscience 2022Quote: ... and 1/20 of the synthesized cDNA was used for each droplet digital PCR (ddPCR) reaction with the ddPCR supermix for probes (Biorad 1863023) using the cycling condition recommended by the manufacture and with the annealing temperature at 66.5°C ...
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bioRxiv - Immunology 2022Quote: ... Real-time polymerase chain reaction (PCR) was performed on a CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA, USA) with SYBR Green PCR mix (Vazyme Biotech) ...
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bioRxiv - Cancer Biology 2022Quote: ... results obtained with Bio-Rad’s iTaq Universal SYBR Green Supermix..The threshold cycle (Ct) for individual reactions were identified using iCycler IQ sequence analysis software (Bio-Rad). β-actin was used for normalization of the data ...
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bioRxiv - Immunology 2020Quote: Total RNA (up to 1 µg per reaction) from BMDM was reverse transcribed with iScript cDNA synthesis kit (Bio-Rad, USA). Quantitative PCR was performed using 1 µl of cDNA ...
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bioRxiv - Molecular Biology 2021Quote: ... The qPCR reaction volume was 20 μL consisting of 1X SsoAdvanced Universal SYBR Green Supermix (Bio-Rad Laboratories Inc., Hercules, CA), 0.5 μM of primers and 2 μL of DNA released from samplers ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were performed in a 96-well transparent plate on a Bio-Rad CFX96 Real-Time PCR machine (Bio-Rad) in 10 µl mixtures each containing 5 µl of Takyon™ ROX SYBR® MasterMix dTTP Blue (Eurogentec ...
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bioRxiv - Molecular Biology 2020Quote: ... All qPCR reactions were performed using a Bio-Rad 1000 Series Thermal Cycling CFX96 Optical Reaction module and iQ SYBR Green Supermix (Bio-Rad). For each primer pair in a given experimental condition ...
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bioRxiv - Microbiology 2021Quote: ... Amplification was done in a standard reaction mixture (Taq DNA Polymerase, Vivantis, Malaysia) following manufacturer’s instruction in a DNA thermal cycler (BioRad T100TM Singapore). DGGE analysis was performed following electrophoresis technique ...
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bioRxiv - Cell Biology 2021Quote: ... Equal concentrations of cDNA were used in the qPCR reactions with iQ or iTaq SYBR Green Supermix (Biorad, #170-8880; #1725120). Reactions were run in triplicate using the BioRad CFX96 Real-time C1000 Touch Thermal Cycler ...