Labshake search
Citations for Bio-Rad :
1901 - 1950 of 2175 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Three technical replicates were assayed per biological sample and analyzed on a C1000 thermocycler with a 384-well qPCR reaction module (Bio-Rad) using primers/probes detailed in Supplementary Table S5 ...
-
bioRxiv - Immunology 2024Quote: ... 20 uL of the reaction mixture was used for droplet generation done by the QX200 Droplet Generator (Bio-Rad Catalog # 1864003). The plate was sealed by a PX1 PCR Plate Sealer (Bio-Rad Catalog #1814000) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 1 ng template library DNA[21] in a total reaction volume of 20 µL using T100 Thermal Cycler (BIO-RAD). The PCR amplification cycle was as follows ...
-
bioRxiv - Cancer Biology 2024Quote: ... Reactions were carried out in duplicates or triplicates with a CFX Connect Real-Time PCR Detection System (Bio-Rad, Munich, Germany). Relative expression was calculated by the ΔΔCt method and normalized against either ribosomal protein lateral stalk subunit P0 (RPLP0 ...
-
bioRxiv - Cancer Biology 2024Quote: ... was added to each sample and heated at 95 °C for 1 hour in a thermal cycler (S1000 with 384-well reaction module, Bio-Rad) set to a constant lid temperature of 110 °C ...
-
bioRxiv - Bioengineering 2024Quote: The outcome of the reaction was analysed using native PAGE (4-20% Mini- PROTEAN TGX Precast Protein Gels, Bio-Rad, 4561096). 1 µL of the retic lysate reaction was diluted with 2 µL 1×PBS and mixed with 2× sample buffer containing 62.5 mM Tris ...
-
bioRxiv - Biochemistry 2024Quote: ... iSAT reactions started after addition of plasmid encoding rrnB under T7 promoter at 4 nM and the reaction was immediately incubated on pre-warmed CFX Connect Real-time System (Bio-Rad) at 37 °C for 90 mins in 96 well plates (Applied Biosystems) ...
-
bioRxiv - Microbiology 2024Quote: ... RNA extracts were tested by real-time RT-PCR with the Menzel and Winter primers (Menzel & Winter, 2021, EPPO, 2022) using iTaq universal probes one-step reaction mix (Bio-Rad) and 1 µl of RNA extract ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... cDNA was synthesized from a reaction mixture containing the RNA template and the supermix with oligonucleotides (iScript reverse transcription Supermix for qRT-PCR, BioRad, USA) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... using a 5 μL PCR reaction volume with SYBR Green supermix (SsoAdvanced™ Universal SYBR® Green Supermix, Bio-Rad, USA). All samples were run in duplicate simultaneously with template free controls ...
-
bioRxiv - Microbiology 2024Quote: ... 1 µg of each RNA sample was used in a 20-µl reverse transcription reaction set up using the iScript Select cDNA synthesis kit (Bio-Rad), together with gene-specific reverse primers (Table S6 ...
-
bioRxiv - Neuroscience 2024Quote: ... The reactions were carried out according to the manufacturer’s protocol using a CFX96 Real-Time System (Bio-Rad, Hercules, CA, USA). PCR amplification and melt curve analysis were performed as detailed in our previous report [48] ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Relative mRNA of genes of interest was quantified by real-time qPCR reactions performed in a CFX384 Touch Real-Time PCR Detection System (thermal cycler, Bio-Rad). The iQ SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Neuroscience 2024Quote: Real-time quantitative PCR reactions were conducted in a 96-well plate using a CFX96 Touch Real-Time PCR System (Bio-Rad). Each reaction was performed in a 10 μL volume containing 1× Luna Universal One-Step Reaction Mix plus 1× Luna WarmStart® RT Enzyme Mix (Cat ...
-
bioRxiv - Biochemistry 2024Quote: ... The reaction was then passed through a P6 Micro Bio-Spin chromatography column (Bio-Rad, 1,000 x g, 4 min, rt), annealed in 1 x annealing buffer and prepared at 100 nM oligonucleotide concentration.
-
bioRxiv - Genetics 2024Quote: ... The cDNA was purified using the MiniElute Reaction Cleanup kit and run on a precast 10% Urea PAGE gel (BioRad #4566033) for 45 minutes at 200V ...
-
bioRxiv - Biochemistry 2024Quote: ... After 1-hour incubation at room temperature the reactions were terminated by addition of 4x Laemmli sample buffer (without β-mercaptoethanol) (BioRad), subjected to SDS-PAGE ...
-
bioRxiv - Bioengineering 2024Quote: ... PCR reactions were set up as followed: 11 μl 2x ddPCR™ supermix for probes (no dUTP) (Bio-Rad, Hercules, CA), 0.2 μl forward primer (100 μM) ...
-
bioRxiv - Biochemistry 2024Quote: ... Multiplex 24 μL ddPCR reactions were prepared by mixing 12 μL of ddPCR supermix (no dUTP; Bio-Rad, catalog no. 1863024), forward and reverse primers for target and reference genes (IDT ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Gene expression was detected using cDNA as a template in a SYBR Green real-time PCR reaction on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad; primer sequences are provided in Table S1) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Then quantitative real-time PCR was performed in triplicate by using AceQ Universal SYBR qPCR Master Mix (Vazyme) for PCR reactions on an iCycler Real-Time Detection System (BioRad, USA).
-
bioRxiv - Cancer Biology 2024Quote: ... The real-time PCR reaction was performed in three technical replicates using the CFX Connect Real-Time PCR Detection System (Bio-Rad), LightCycler® 480 SYBR Green I Master (Roche) ...
-
bioRxiv - Biochemistry 2024Quote: ... a reaction mixture (10 μL total volume) was prepared by mixing 5 μL of iQ SYBR Green mix (Bio-Rad, 1708882), 0.3 μL of 10 μM forward primer ...
-
bioRxiv - Cell Biology 2024Quote: ... and 0.025 u/µL SeqAmp DNA polymerase in the 2.5 µL final reaction) using a Mantis liquid handler and then performing PCR on a BioRad C1000 384-well thermal cycler (Bio-Rad) with the following program ...
-
bioRxiv - Cell Biology 2024Quote: ... Each 22 µL ddPCR reaction consisted of 11 µl 2x ddPCR SuperMix for probes (no dUTP) (Bio-Rad, Hercules, California, USA), 909 nM of each primer ...
-
bioRxiv - Genetics 2024Quote: ... Two technical duplicates were performed for each biological replicate and reactions were run in a CFX Connect Real-Time PCR Detection System (Bio-Rad). 16S rRNA and β-actin mRNAs were used as reference genes to normalize the results relative to the expression of bacterial and human genes ...
-
bioRxiv - Genetics 2024Quote: ... Three technical replicates were performed for each biological replicate and reactions were run in a CFX Connect Real-Time PCR Detection System (Bio-Rad). Calculation of the mtDNA content relative to the genomic DNA was performed using the following equations ...
-
bioRxiv - Evolutionary Biology 2024Quote: We used Promega GoTaq polymerase in 25µl reactions for PCR amplifications with Cari-COI-1F and Cari-COI-1R and iProof High Fidelity DNA Polymerase (Bio-Rad) in 40µl reactions for PCR amplifications with COI-1f and COI-1r using 1 µl of the DNA template for both ...
-
bioRxiv - Cell Biology 2020Quote: ... The membranes were treated with ECL chemiluminescence detection reagent (GE healthcare, product # RPN2106V1 and RPN2106V20 and imaged using the Bio-Rad Chemidoc system (Bio-Rad Laboratories). Densitometry was done using ImageLab software (Bio-Rad Laboratories ...
-
bioRxiv - Biophysics 2020Quote: ... We visualized the transcription products by electrophoresis in a denaturant gel (23% polyacrylamide, 7 M urea) run at 800V for 2 h (Bio-rad, Hercules, CA, USA).
-
bioRxiv - Microbiology 2021Quote: ... 50 μl of cells were mixed with PCR products and transferred to a precooled 2 mm gap electroporation cuvette (Bio-Rad Laboratories, Hercules, CA). A BTX Gemini X2 instrument was used for electroporation with the following settings ...
-
bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped using glacial acetic acid and the N-glycans desalted sequentially using strong cation exchange (AG 50W X8, Bio-Rad), C18 and porous graphitised carbon (PGC ...
-
Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Incorporation of radioactive amino acids was determined by liquid scintillation counting and normalized to the cellular protein content (quantified with DC Protein Assay (Bio-Rad)).
-
bioRxiv - Microbiology 2023Quote: ... 12 % SDS-PA gels were fixed with fixation solution (40 % ethanol, 10 % acetic acid) o/n and stained in Flamingo fluorescent protein dye (Bio-Rad) for up to 6 h and imaged with the ChemiDoc (Bio-Rad) ...
-
bioRxiv - Cell Biology 2023Quote: ... Gels were then stained with Coomassie Blue solution, followed by de-staining (Methanol, Acetic Acid solution) and visualised by using Gel Doc™ XR+ system (BioRad).
-
bioRxiv - Cancer Biology 2023Quote: ... The reaction was stopped by 10% acetic acid and the gel was washed with water for visualization by ChemiDoc Imaging system (Bio-Rad).
-
bioRxiv - Cancer Biology 2023Quote: ... and then the gel was washed in destain buffer (8% acetic acid) until the background was washed off for visualization by ChemiDoc Imaging system (Bio-Rad).
-
bioRxiv - Molecular Biology 2022Quote: ... using 10 mM CAPS buffer (3-[cyclohexylamino]-1-propanesulfonic acid [pH 11]) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) according to protocol provided by the manufacturer ...
-
bioRxiv - Neuroscience 2023Quote: ... All lysates were normalized using bicinchoninic acid (BCA; Pierce BCA Protein Assay Kit) and combined with 4x Laemmli buffer (Bio-Rad). We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... Antibiotic disk content used in the assays were cefotaxime 30 μg and amoxicillin + clavulanic acid 30 μg (20 μg + 10 μg; all from Bio-Rad).
-
bioRxiv - Microbiology 2022Quote: ... Reaction was developed using a freshly prepared TMB substrate solution and stopped with 1M Sulfuric acid before determining the OD450 using a microtiter plate reader (iMark mic, Bio-Rad).
-
bioRxiv - Microbiology 2022Quote: ... methanol in 1X TBE buffer with 10% (v/v) acetic acid) and RNA was visualised using the Silver Stain Plus Kit (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: Intracellular and soluble-secreted proteins were Deoxycholate/Trichloroacetic acid– precipitated and separated by SDS-PAGE on gradient 4-20% PROTEAN TGX Stain-Free Gels (Bio-rad) and proteins were transferred to polyvinylidene difluoride membranes within Trans-Blot Turbo Transfer Packs (Bio-rad ...
-
bioRxiv - Cell Biology 2023Quote: ... for protein extracted from cultured cells or brain sections and micro bicinchoninic acid (microBCA) for protein extracted from EVs according to the manufacturer’s instructions (Bio-Rad Laboratories).
-
bioRxiv - Biophysics 2022Quote: ... Gels were fixed for 10min (50% methanol, 10% acetic acid) and stained with Coomassie Blue for imaging on a gel doc (Bio-Rad). Band intensities were quantified using Image Lab (Bio-Rad ...
-
bioRxiv - Genetics 2023Quote: ... China) using TBE (65 mM Tris-HCl, 27 mM boric acid, 1 mM EDTA, pH 9; Bio-Rad, Hercules, CA, USA) as the buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... After gel polymerization was performed pre-electrophoresis of empty gel in 5% acetic acid (running buffer) for 1-1.5h/150 V in reverse polarity of electrophoretic BioRad system (Bio-rad, USA). The running buffer was discarded ...
-
bioRxiv - Cancer Biology 2024Quote: ... submerged in blocking buffer (40% ethanol and 10% acetic acid in distilled water) with shaking for 15 min and stained with coomassie blue dye (Bio-Rad) by gentle shaking overnight ...
-
bioRxiv - Plant Biology 2024Quote: ... The biotin-labelled DNA probes were detected using the Chemiluminescent Nucleic Acid Detection Module Kit (ThermoScientificTM; USA) and pictured under BioRad Gel DocTM XR (BioRad; USA).
-
bioRxiv - Neuroscience 2024Quote: ... and then rinsed with a solution of 40% ethanol/10% acetic acid before being air dried overnight and imaged on Chemidoc (Bio-Rad).