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Citations for Bio-Rad :
1701 - 1750 of 2175 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... each ddPCR reaction mixture was loaded into the sample well of an eight-channel disposable droplet generator cartridge (Bio-Rad). A volume of 60 μl of droplet generation oil (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: ... followed by reverse transcription and sample indexing using a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (BioRad). After breaking the GEMs ...
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bioRxiv - Genetics 2023Quote: ... followed by linear PCR as described in the protocol using a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (BioRad). After breaking the GEMs ...
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bioRxiv - Cancer Biology 2023Quote: ... were thawed on ice for 20 min prior to addition of 1 μL of Gibson reaction and electroporation (1800 V) in a MicroPulser Electroporator (Biorad). 975 μL of pre-warmed recovery medium were added and the bacteria were incubated 37 °C at 250 rpm for 1 hour ...
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bioRxiv - Plant Biology 2023Quote: ... Each RT-qPCR reaction consisted of 1x SsoAdvancedTM Universal Inhibitor-Tolerant SYBR® Green Supermix (Bio-Rad, Hercules, CA, USA), 2.5 μM of each gene-specific primer ...
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bioRxiv - Cell Biology 2023Quote: ... One hundred nanograms of cDNA were amplified in duplicates in each 40-cycle reaction using a CFX 384 Real Time PCR System (BioRad) with annealing temperature set at 60°C ...
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bioRxiv - Physiology 2023Quote: ... 500 ng RNA was reverse transcribed using HiScript III 1st Strand cDNA Synthesis Kit followed by qPCR reactions using SYBR Green master mix and analyzed by Biorad CFX Touch Real-Time PCR Detection System (SIA-PCR008) ...
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bioRxiv - Genomics 2023Quote: ... Each ddPCR reaction was set up by adding the following: 10 uL of 2X ddPCR Supermix (no dUTP; BioRad #1863023), 2 uL of 10 uM ITR_Forward Primer ...
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bioRxiv - Molecular Biology 2023Quote: ... quantitative real-time PCR was carried out at 20µl reaction volume and performed in CFX96 Touch Real-Time PCR Detection System (Bio-Rad). The amplification protocol started with denaturation step at 95°C for 30s ...
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bioRxiv - Genetics 2023Quote: ... qRT-PCR reactions were assembled using SsoFast™ EvaGreen® Supermix with Low ROX (Bio-Rad Laboratories, Inc., Hercules, CA) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The ddPCR reaction mixture consisted of 12.5 µl of a 2× ddPCR Supermix for Probes no dUTP (1863024; Bio-Rad), 1.25 µl of each 20× primer-probe mix (Table 2) ...
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bioRxiv - Pathology 2024Quote: ... Total reaction volume was 10 µl and contained 5 µl of iTaq Universal SYBR green one-step kit (Bio-Rad), 300 nM of forward and reverse primers (Table S5 ...
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bioRxiv - Cell Biology 2024Quote: ... and all reactions were performed in duplets in a PCR cycler (CFX 96-qPCR cycler / CFX 384-qPCR, Bio-Rad). The RT- negative control for cDNA synthesis ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction mixture was prepared using a custom ddPCR assay (#10031276 for FAM and #10031279 for HEX; Bio-Rad Laboratories) and ddPCR Supermix for Probes (no dUTP ...
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bioRxiv - Developmental Biology 2024Quote: ... For each condition 3 biological replicates with two technical replicate RT-qPCR reactions were run on a CFX96 Connect Real-Time System (BioRad). The number of il1b ...
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bioRxiv - Immunology 2024Quote: ... Quantitative polymerase chain reaction (qPCR) analysis was performed with SsoAdvanced™ Universal SYBR® Green Supermix (#1725271; Bio-Rad Laboratories) and the following primers:
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bioRxiv - Molecular Biology 2024Quote: ... were used in triplicate 20 µL reactions of quantitative PCR using the iTaq Universal SYBR Green Supermix (Bio-Rad #1725121) in a CFX Connect Real-Time PCR Detection System (Bio-Rad #1855201 ...
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bioRxiv - Cell Biology 2024Quote: ... USA) was used and reactions were carried out in a CFX Connect Real-Time PCR Detection System (Bio-Rad, USA). Reaction procedures were set to an initial heating step of 95 °C for 3 min as well as 40 cycles of denaturing at 95 °C for 10 s followed by combined annealing-extension at 60 °C for 40 s ...
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bioRxiv - Immunology 2024Quote: ... and transferred to a thermal cycler (C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module, 1851197, Bio-Rad). Reactions conditions were ...
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bioRxiv - Immunology 2024Quote: ... cDNA was diluted 2-fold and 1 μL was used for each PCR reaction with the Biorad qRT PCR mix using a CFX Maestro (Biorad) machine ...
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bioRxiv - Microbiology 2024Quote: ... The PCR reaction mixture (25 μL total) consisted of 12.5 μL of 2× SYBR Green PCR Master Mix (Bio-Rad), 300 nmol each of forward and reverse primers ...
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bioRxiv - Microbiology 2024Quote: ... About 88.88 ng/µL of total RNA was added to the cDNA synthesis reactions using the iScript Select Kit (Bio-Rad).
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bioRxiv - Microbiology 2024Quote: ... Reactions were run with SsoAdvancedTM Universal SYBR® Green Supermix on CFX96 Touch Real-Time PCR Detection System (Bio-Rad) with CFX Manager (v3.1 ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was incubated (15 min, RT) and the absorption was measured using a platereader (570 nm, Biorad Benchmark Plus). Afterwards ...
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bioRxiv - Plant Biology 2024Quote: ... 1 µg of RNA was used in a 20-µL reverse transcription reaction according to the manufacturer’s instructions (iScript; Bio-Rad) followed by a 5-fold dilution with ultrapure water ...
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bioRxiv - Physiology 2024Quote: ... Subsequently RT-qPCR reactions were performed using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad, Veenendaal, the Netherlands) and the following parameters ...
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bioRxiv - Cell Biology 2024Quote: ... reactions were performed in duplicate with primers used at 10μM in combination with the iQ SYBR green Supermix (Bio-Rad) in a Bio-Rad thermocycler ...
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bioRxiv - Biochemistry 2024Quote: DNA at selected regions of the genome were quantitated in isolated and desalted DNA fractions by quantitative polymerase chain reaction (qPCR) on the iCycler platform using the iTaq SYBR green supermix (BioRad). Primer pairs amplifying replication origins and corresponding adjacent background sites were synthesised by Sigma-Aldrich and used in PCR reactions at 0.5 µM for each primer ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR reactions were carried out in a CFX96 detection system (Thermal-Cycler C1000, Bio-Rad Laboratories, Hercules, CA, USA). The list of primers used is given in Table S7.
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bioRxiv - Cancer Biology 2024Quote: ... real-time quantitative polymerase chain reaction (RT-qPCR) was performed (CFX Connect and iTaq Universal SYBR Green SMX, Bio-Rad). All PCR reactions were performed with primers (Supplemental Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The qRT-PCR reactions were performed using the CFX96 Real-Time PCR System [C-1000 Touch Thermal Cycler] (BIO-RAD) and relative gene expressions were calculated using the ΔΔCT method normalized to S18 housekeeping gene ...
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bioRxiv - Genomics 2020Quote: ... and the products from the second round of amplification were resolved on an agarose gel and stained with ethidium bromide (Bio-Rad, Hercules, CA).
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bioRxiv - Developmental Biology 2020Quote: ... followed by Super Signal@ West Femto chemiluminescent substrate (Pierce, Rockford, IL, USA) and the products were recovered by Laser 4000 chemiluminescent system (Bio-Rad, CA, USA). Finally ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products (700 ng) were introduced into electrocompetent cells of JW0452 carrying pKD46 by electroporation using a Gene Pulser XcellTM (BioRad, Hercules, CA, USA). The occurrence of recombination at the expected site was confirmed by PCR using primers CAT-584 and BW25113_2815723f (Table 2) ...
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bioRxiv - Neuroscience 2024Quote: ... SYBR green detection of PCR products was conducted in real time using a MyiQ single-color detection system (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2024Quote: ... The assembled products were mixed with electrocompetent cells and transformed into electrocompetent cells in a 2-mm Gene Pulser cuvette (Bio-Rad, 165-2086) using an electroporation instrument (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... The images of the Western blot products were collected by a gel imaging system (BIO-RAD GelDoc 2000, Bio-Rad Laboratories, Inc. USA) and analyzed by Image Pro Plus 6.0 (MEDIA CYBERNETICS ...
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bioRxiv - Immunology 2023Quote: ... The amplified products were analyzed on a 2% agarose gel stained with SYBR safe and imaged using the Gel-documentation system (Bio-Rad, Hercules, CA).
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bioRxiv - Microbiology 2024Quote: ... the fluorescence derived from the incorporation of SYBR® Green Dye into the double-stranded PCR products was measured at the end of each cycle using the SsoAdvanced Universal SYBR® Green Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Microbiology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was stopped with 1N sulfuric acid and absorbance was measured at 450 nm in the iMarkTM Microplate Absorbance Reader (BioRad).
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bioRxiv - Molecular Biology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... and destained with 30% methanol,10% glacial acetic acid in double distilled water and subjected to film by ChemiDoc systerm (BioRad).
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bioRxiv - Physiology 2020Quote: ... Samples and standards were incubated with bicinchonic acid for 30 minutes prior to measuring absorbance as 540 nm using an iMark microplate absorbance reader (BioRad).
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bioRxiv - Microbiology 2022Quote: ... A Bio-Rad Aminex HPX-87H HPLC column for organic acid analysis (300 × 7.8 mm; Bio-Rad Hercules, CA, USA) was equilibrated with 5 mM H2SO4 (Titrisol ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... The gel was then destained for 15 minutes in 7.5% (v/v) acetic acid before being imaged on a Chemidoc imaging system (Bio-Rad). For western blots ...
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bioRxiv - Biochemistry 2022Quote: ... Gels were Coomassie stained and destained with 10% acetic acid prior to imaging on a ChemiDoc MP imaging system (BioRad).
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed on a 4-15% polyacrylamide NuPage Bis-Tris gel (invitrogen) in 1x morpholineethanesulfonic acid (MES) SDS running buffer (invitrogen) or on a 4-20% polyacrylamide Criterion gels (BioRad) in 1x Tris/glycine/SDS buffer (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... protein concentration was measured using a bicinchoninic acid (BCA) protein assay (Pierce) or Bradford assay (Bio-Rad, Cat. No. 5000006). Proteins were prepared in 1× Laemmli buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction was resolved by electrophoresis on a 6% non-denaturing polyacrylamide gel in TBE (100 mM Tris, 90 mM boric acid, 1 mM EDTA) buffer and visualised by ChemiDoc (BioRad) using SYBR Gold stain or imaging in the 6FAM ...