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Citations for Bio-Rad :
1851 - 1900 of 2175 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The 10 µL cDNA reaction was diluted to 200 µL and 3 µL was used with iTaq Universal SYBR Green Supermix (Bio-Rad) and 670 nM of primer pairs (Table S4 ...
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bioRxiv - Plant Biology 2022Quote: ... The dicing reactions were performed at 37 °C for 30 min and stopped by adding 1 volume TBE-Urea sample buffer (Bio-Rad), heating at 95 °C for 10 min and quickly chilling on ice for 2 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The RT reactions were amplified with primers for target RNA and ACTIN (Supplemental Table S2) using iTaq Universal SYBR Green (Biorad, 1725120), and quantified on (Biorad CRX Connect Real-Time PCR Detection System ...
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bioRxiv - Cancer Biology 2024Quote: ... and 500 ng RNA was reverse transcribed into cDNA in a 10 μl reaction using the iScript cDNA synthesis kit (Bio-Rad). cDNA was then diluted 1:10 and 4 μl was used for quantitative RT-PCR using the LuminoCt SYBR Green master mix on a Roche LightCycler 480 machine ...
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bioRxiv - Biochemistry 2024Quote: ... and 5 to 20 mM imidazole (wash-buffer) until the eluate was protein-free as judged by the lack of reaction with Bradford dye reagent (Bio-Rad). The calpain-3-titin/I81-I83 complex was eluted with 300 mM imidazole in wash-buffer ...
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bioRxiv - Biophysics 2024Quote: ... The DNA strands at 1 mM concentration were incubated with 10 mM of BG-GLA-NHS and reactions were cleaned up using a Bio-Spin P-30 column (Bio-rad).
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bioRxiv - Cancer Biology 2024Quote: ... Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) assays were conducted on the CFX96 real-time detection system (Bio-Rad, USA) with the SYBR Green PCR Mix (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... cDNA was amplified in a 10 μL reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad, Hercules, CA) and 5 μM each of gene-specific forward and reverse primers ...
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bioRxiv - Genomics 2024Quote: ... 10 ng of cDNA was used per PCR reaction with Perfecta SYBR Green Fastmix (Quanta BioSciences, 95072) using the CFX96 Real-Time PCR Detection System (Bio-Rad). All amplicon products were verified by melting curve analysis ...
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bioRxiv - Immunology 2024Quote: ... quantitative real-time polymerase chain reaction (qRT-PCR) using gene-specific primers and universal SYBR Green Supermix (#1725274, Bio-Rad Laboratories) was performed ...
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bioRxiv - Immunology 2024Quote: ... 20 µl of each reaction mix and 70 µl of oil were converted to droplets with the QX200 droplet generator (Bio-Rad). Droplet-partitioned samples were then transferred to a 96-well plate ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of synthesized cDNA was firstly diluted in a 1:5 ratio and used in a 20 µL reaction volume using the CFX96 Touch Real-Time PCR Detection System (BioRad, USA) with the following thermal cycling conditions ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR reactions were carried out using the SYBR Green PCR master mix and ROX Passive Reference Dye (Bio-Rad) with Micro-Amp Optical 384-well plates ...
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bioRxiv - Cell Biology 2024Quote: ... We produced droplets pipetting 20 µl of the PCR reaction mix into single well of a universal DG8TM cartridge for droplets generation (Bio-Rad). 70 µl of droplet generation oil were also added in each well next to the ones containing the samples ...
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bioRxiv - Plant Biology 2023Quote: ... Reverse transcription reaction was conducted using 0.5 µg of total RNA and 2 µl of iScript™ Reverse Transcription Supermix (Bio-Rad) in 10 µl reaction volume ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 µl of the diluted solution was used as template in a 15 µl reaction with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). qPCR was performed using CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... A volume of 40 µl of generated droplets per reaction was then transferred to a 96-well plate and foil-sealed (1814040; Bio-Rad) with a thermal plate sealer (PX1 ...
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bioRxiv - Microbiology 2023Quote: ... Each assembled ddPCR reaction mixture was then loaded into the sample well of an eight-channel disposable droplet generator cartridge (Bio-Rad). A volume of 70 μL of droplet generation oil (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: qPCR was run with cDNA previously generated from rescue experiments in a 10 µl reaction using iTaq Universal SYBR Green Master Mix (BioRad 1725121) with readout using the Applied Biosystems ViiA 7 real-time PCR system with standard cycling parameters (50°C for 1 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR (qPCR) was performed in a 10 µL reaction that contained 5 µL SsoAdvanced™ Universal SYBR Green Supermix (BioRad), 1 µL H2O ...
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bioRxiv - Microbiology 2023Quote: ... mouse cDNA was analyzed in a 10-μl reaction mix consisting of 1x iQ SYBR® Green Supermix (Bio-Rad, USA), 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Chemiluminescent reaction was performed with SuperSignal West Dura substrate (Thermo, 34076).Images were acquired with a ChemiDoc XRS+ system (Bio-rad) and quantified using Image Lab software (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: Immunoassays were performed with 25 μl of conditioned medium or tissue lysate each reaction using a Bio-Plex 200 System (Bio-Rad) with Milliplex Map Mouse Cytokine/Chemokine Panel I (Millipore ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from PMVECs and Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 µl of a 10X diluted cDNA sample was then used in the qPCR reaction with SsoAdvanced Universal SYBR Green Supermix (Bio-rad). Primers were designed using Primer3 software (v4.1.0) ...
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bioRxiv - Microbiology 2022Quote: ... The digital droplet PCR (ddPCR) reaction mixture consisted of 12.5 µL of a 2X ddPCR Supermix for Probes no dUTP (Bio-Rad: 1863024), 1.25 µL of each 20X primer-probe mix ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR amplifications were carried out at least in duplicate in a 25 µL reaction volume on a CFX96 instrument using the CFX manager software (Bio-Rad). Each PCR reaction mixture was composed of 12.5 µl PCR mix (Bio-Rad Sso advanced mix SYBR Green) ...
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bioRxiv - Bioengineering 2022Quote: ... approximately 5 ng of RNA converted to cDNA was used per 10μL well reaction in the Wound Healing PCR Array (Bio-Rad, 10034601). Real-time PCR reaction was prepared using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: ... and the Phusion Green Hot Start II High Fidelity PCR Master Mix (Thermo-Fisher-Scientific) according to the manufacturer’s instructions in a 25 μl reaction on a C1000 Thermo Cycler (Biorad, Hercules, USA). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... Amplification was performed through polymerase chain reaction (PCR) in a Bio-Rad C1000 TouchTM Thermal Cycler (BIO-RAD, Hercules, CA, USA). The V4 region of the 16S rDNA gene was amplified using dual-index (forward and reverse ...
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bioRxiv - Pathology 2022Quote: ... Whole bee specimens were extracted in trizol and then RNA was used to produce cDNA using single reaction reverse transcriptase according to manufacturer specifications (BioRad, IScript). Total viral cDNA was quantified using real time qPCR and a 10 fold dilution series of prepared standards exactly as described in Posada-Florez et al.12.
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bioRxiv - Bioengineering 2023Quote: ... 1 µL of the formed cDNA was used in 10 µL reactions using the SsoAdvanced universal SYBR green supermix (Bio-rad) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was then diluted 1:100 and 1.5 µL was use per 15 µL reaction with iTaq Universal SYBR Green Supermix (Bio-Rad # 1725122) and 250 nM primers (final ...
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bioRxiv - Physiology 2023Quote: ... was used to run the quantitative polymerase chain reaction (qPCR) using the CFX 380 Real-Time system C1000 TM Thermal Cycler (Bio-Rad), with the following parameters ...
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bioRxiv - Genomics 2023Quote: ... 2.0 μL of cDNA was used per PCR reaction with Perfecta SYBR Green Fastmix (Quanta BioSciences, 95072) using the CFX96 Real-Time PCR Detection System (Bio-Rad). All primers were designed to be highly specific using NCBI’s primer blast tool and amplicon products were verified by melt curve analysis ...
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bioRxiv - Cell Biology 2023Quote: 5 to 10 ng of cDNA were used to perform quantitative polymerase chain reaction using 1X SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). All RT-qPCR reactions were performed in a CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... The reverse transcription was conducted using 500 ng of RNA in a 20 µl volume reaction mixture of iScript cDNA Synthesis Kit (Bio-Rad). 2.5 µl of 1:10 diluted cDNA was added to 7.5 µl of Sensi-FAST SYBR No-ROX (Bioline ...
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bioRxiv - Physiology 2024Quote: ... Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was performed using a CFX96 Real-Time PCR Detection System (Bio-Rad) with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... qPCR reactions were performed in a 96-well transparent plate on a Bio-Rad CFX96 Real-Time PCR machine (Bio-Rad) in 10 µl mixtures each containing 5 µl of Takyon™ ROX SYBR® MasterMix dTTP Blue (Eurogentec ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of each diluted RT and NRT sample was added to a 22 µL reaction mixture containing 11 µL of QX200TM ddPCR TM Evagreen Supermix (Bio-Rad) and 1.1 µL of each 2 µM ddPCR primers (Table S6) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation at 95°C for 60 minutes in a thermal cycler (S1000 with 384-well reaction module, Bio-Rad). To this ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
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bioRxiv - Microbiology 2024Quote: 1 μg of total RNA extracted from the biofilm fraction of two days old cholesterol-attached biofilms was used for a reverse transcription reaction with iScript Supermix (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Gene expression was analyzed by qPCR in 20ng of cDNA in triplicate 10 µl reactions using SYBR Green Taq MasterMix (Bio-Rad), following the manufacturer’s instructions in a CFX-384 Real-Time PCR thermocycler (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted 1/5 and quantified by RT-qPCR in 10 μL final reactions using 2X iTaq Universal Sybr Green Supermix (#1725125, Bio-Rad) in CFX96 Touch Real-Time PCR machines (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... One microgram of total RNA was used for a reverse transcriptase reaction using iScript cDNA Synthesis Kit (Catalog #170-8891; Bio-Rad). RT-qPCR analysis was conducted using iQ SYBRGreen Supermix (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... was performed with 100 ng of cDNA in a 20-μL reaction mixture containing SsoAdvanced universal SYBR green supermix (#1725274; Bio-Rad) using phagocytosis PrimePCR plates (#10047255 ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed using 2 uL of diluted cDNA in a 20-µL reaction with SYBR Green dye polymerase mix (SsoAdvanced; Bio-Rad) in white-walled plates in a CFX96 thermocycler (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription reactions were performed using 4 µL of iScript at room temperature with 1 µg-1 pg of RNA template (Bio-Rad) in a final volume of 20 μL ...
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bioRxiv - Genetics 2024Quote: ... Reverse transcription and library preparation were performed on a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad). Amplified cDNA and final libraries were evaluated on an Agilent Tapestation using a High Sensitivity D1000 ScreenTape (Agilent Technologies) ...