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Citations for Bio-Rad :
1951 - 2000 of 2175 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The 6xHis-tagged G3BP1-GFP protein was purified by loading the supernatant into a nickel-nitrilotriacetic acid (Ni-NTA) column (BioRad, India) pre-equilibrated with buffer ...
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bioRxiv - Microbiology 2020Quote: ... 22 μL reactions contained 11 μL of 2x ddPCR™ Supermix for Probes (No dUTP) (Bio-Rad Laboratories, Inc., Cat. No. 1863023), 0.4 μM of all probes and primers ...
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bioRxiv - Molecular Biology 2021Quote: ... The absolute amount of target DNA copy per reaction was calculated based on Pearson’s correlation method using QuantaSoft™ ddPCR analysis software (Bio-Rad, USA). PCR reactions for each individual sample were performed in duplicate.
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bioRxiv - Neuroscience 2021Quote: ... Transcript levels were assessed via real-time quantitative polymerase chain reaction (qPCR) amplifications using an iQ SYBR Green Supermix (BioRad, Hercules, CA) with primers listed in Table 4-1 on a CFX96 system (BioRad) ...
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bioRxiv - Neuroscience 2021Quote: ... CLN5 transcripts were measured from the control and CLN5i neurons RNA by quantitative polymerase chain reaction[13] using an iTaq Universal Probes one-step kit (Bio-Rad, 1725141) on a Roche 96-well thermocycler ...
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bioRxiv - Immunology 2020Quote: ... was used as a template for quantitative real-time PCR (qPCR) and the reaction mixture was prepared using iTaq™ Universal SYBR® Green Supermix (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... All RTqPCR reactions were performed in triplicate using TaqMan probes™ in combination with CFX96 Touch™ Real-Time PCR Detection System (BioRad), paired with CFX Maestro™ Analysis Software.
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bioRxiv - Cancer Biology 2020Quote: ... Droplets were produced pipetting 20µl of the PCR reaction mix into single wells of a universal DG8™ cartridge® for droplets generation (Bio-Rad). 70µl of droplet generation oil® was also added in each well next to the ones containing the samples ...
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bioRxiv - Evolutionary Biology 2020Quote: ... First-strand cDNA was synthesized from 200 ng of total RNA in a 20-µL reaction volume using oligo-dT primers and iScript RT (Bio-Rad Laboratories).
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bioRxiv - Evolutionary Biology 2021Quote: ... gene were amplified by polymerase chain reaction (PCR). The Touchdown PCR (Don et al. 1991) was performed in a PTC-220 PCR thermal cycler system (Bio-Rad, USA). The 40 μl reaction for PCR contained 2.4 μl template DNA ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... 10μl of EMSA binding reactions were loaded on 5% nondenaturing polyacrylamide Mini-PROTEAN TBE gels (Catalog #4565015, Bio-Rad Laboratories, Hercules, CA) for electrophoretic analyses ...
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bioRxiv - Neuroscience 2020Quote: ... levels were quantified by real time polymerase chain reaction (RT-PCR) using SYBR® Green DNA intercalating dye and master mix (Bio-Rad) and the ABI 7900HT PCR machine ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions were performed with 2 × Taq Master Mix (Vazyme, Nanjing, China) on a Bio-Rad Thermal Cycler (Bio-Rad, Hercules, CA, USA) using the following procedure ...
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bioRxiv - Biochemistry 2021Quote: ... For analysis of isolated RNA semi-quantitative reverse transcription–polymerase chain reaction (RT) PCR was performed with the iScript™ cDNA Synthesis Kit (BIORAD, Germany) according to the manufacturer’s instructions using 500ng of isolated RNA ...
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bioRxiv - Biochemistry 2020Quote: ... Real time polymerase chain reaction (RT-PCR) was performed with the CFX96 or CFX384 Touch(tm) Real-Time detection system (Bio-Rad Laboratories), using a SensiMix(tm ...
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bioRxiv - Plant Biology 2020Quote: ... The reactions were carried out in a thermal cycler (Single Block Alpha Unit, DNA Engine®, Bio-Rad Laboratories, Berkeley, CA, USA) with an annealing temperature of 55 °C ...
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bioRxiv - Plant Biology 2020Quote: ... or 1 μl (for the rest) of a 1/2 dilution of the cDNA was added to a reaction containing 1X of SsoFast EvaGreen (Bio-Rad, USA), 0.5 μM Forward and Reverse primers (Table 2) ...
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bioRxiv - Plant Biology 2020Quote: ... we used 2 µl of a 1/5 dilution of the cDNA obtained as above in a reaction containing 5 µl of SsoFast EvaGreen (Bio-Rad, USA), 0.5 µl of Forward primer (10 µM ...
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng total RNA was reverse-transcribed to cDNA in a 20 μl reaction with 4 μl iScript reverse transcription supermix (Bio-Rad #1708841) at 25 °C for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... 4 ul of diluted cDNA (1:40 dilution rate) were used for each reaction with 5 ul SsoAdvanced Universal SYBR® Green Supermix (BIO-RAD) and 1 ul of primer mix (5uM) ...
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bioRxiv - Neuroscience 2022Quote: ... Real-Time quantitative Polymerase Chain Reaction (RT-qPCR) was performed using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) using 3 ng of cDNA per reaction and Sensifast SYBR No-ROX mix (Bioline Corporation ...
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bioRxiv - Immunology 2022Quote: ... GEM-RT incubation was performed in a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad; cat. no. 1851197): 53°C for 45 min ...
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bioRxiv - Microbiology 2022Quote: ... All the cDNA products were utilized for the quantitative real-time PCR reaction using the GoTaq qPCR Master Mix on a CFX96 real-time PCR Detection System (Bio-Rad, CA). Primer pairs were designed using Universal Probe library website and shown in Table S4 ...
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bioRxiv - Genetics 2022Quote: ... We generated droplets from 20 µL of reaction mix and 70 µL of Droplet Generation Oil using the QX200 Droplet Generator (Bio-Rad Laboratories). We ran polymerase chain reactions in a Bio-Rad C1000 Touch Thermal Cycler ...
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bioRxiv - Microbiology 2020Quote: ... and subsequently underwent thermocycling on the C1000 Touch™ Thermal Cycler with a 96–Deep Well Reaction Module (Bio-Rad Cat#1851197). Thermal cycling conditions with a 2°C ramp rate were ...
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bioRxiv - Developmental Biology 2021Quote: ... using the whole SingleShot™ Cell Lysis Kit (SingleShot™ Cell Lysis Kit, 100 x 50 µl reactions Cat# 1725080, Bio-Rad) as follows ...
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bioRxiv - Neuroscience 2021Quote: ... SYBR ROX green qPCR reactions were performed in triplicate and data were collected using a BioRad CFX Connect Real-Time PCR Detection System (BioRad, Hercules, CA). Reactions were denatured at 95°C for 10 min then subjected to 95° C for 15 sec and 60° C for 1 min for 40 cycles ...
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bioRxiv - Genomics 2020Quote: ... 5 µL of cDNA was pre-amplified in a 12-cycle PCR reaction using the Sso Advanced PreAmp Supermix (Bio-Rad, USA) in a 50 µL reaction ...
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bioRxiv - Biophysics 2021Quote: ... Table A in S1 Text) in a single reaction using a CFX Opus real-time qPCR machine (CFX96, Bio-Rad Laboratories, CA). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... The synthetic ssDNA oligo was converted to dsDNA by primer extension reaction at 60° C using iProof DNA polymerase (Bio-Rad, USA). The resulting duplex was cloned between the EcoRI and HindIII sites of the pUC57 vector ...
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bioRxiv - Neuroscience 2022Quote: ... All reactions were performed using primers listed in the supplementary table 3 and the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). Relative expression levels were determined according to the ΔΔCt method with the human housekeeping gene ABELSON as endogenous control for normalization as previously described (Beillard et al. ...
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bioRxiv - Neuroscience 2022Quote: ... and cDNA aliquots were analyzed in simultaneous real-time quantitative polymerase chain reactions using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Canada) and a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... and stopped at each of the above-mentioned time points by loading an 150 µl aliquot of the reaction mixture to an anion exchange resin column (acetate form, 100-200 mesh, Dowex AG1-X8 Resin, Bio-Rad, UAS). The resin column was previously equilibrated five times using 150 mM sodium acetate (pH=7.5) ...
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bioRxiv - Molecular Biology 2020Quote: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays were performed using a C1000 TouchTM Thermal Cycler (Bio-Rad, Hercules, CA, USA). The reaction system included 5 μL SYBR® Premix Ex TaqTM (Takara ...
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bioRxiv - Microbiology 2021Quote: ... Final extension was at 72°C for 7min and the reaction was stopped by holding tubes at 4°C until removed from the thermal cycler (MyCycler™ Thermal Cycler BIORAD, USA). The PCR products were electrophoresed in a 1.5% agarose gel stained with SYBR Safe DNA Gel stain (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Each PCR reaction (10 μL final volume) contained 5 μL SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA), 100 ng of cDNA ...
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bioRxiv - Genetics 2020Quote: ... the reaction droplets were transferred into a 96-well plate and sealed with foil PCR plate heat seal (Bio-rad #181-4040) and proceeded to thermal cycling with profiles of 95 °C (10 min ...
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bioRxiv - Genomics 2021Quote: ... Bead barcoding and sample indexing were performed in a C1000 Touch™ Thermal cycler with a 96-Deep Well Reaction Module (1851197, Bio-Rad): 37°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... Approximately 20ng of cDNA were used as template for qPCR reactions using SSOAdvanced Universal SYBR Green Supermix following manufacturer’s instructions (BioRad; catalog no.1725271). Following primers were used for detection of cellular genes by qPCR:
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bioRxiv - Immunology 2020Quote: ... Conjugate DNA was measured by qPCR amplification from 1 μL of lysate in a 10 μL reaction containing 5 μL of iTaq Universal SYBR Green Supermix (Bio-Rad, 1725125) and 5 pmol of each primer (Supplementary Table 1) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Reverse transcription reactions were performed to synthesize cDNA from 200 ng of total RNA using the iScript™ Select cDNA Synthesis Kit (Biorad, CA). Real-time quantitative PCR (qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: ... Fifty (50) μl polymerase chain reaction (PCR) mixtures were carried out in a T100™ Thermal Cycler (Bio-Rad Laboratories, Hercules, CA) using 100 ng of total DNA template along with AmpliTaq® DNA Polymerase reagents (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... The reaction rate and total protein concentration (determined by the method of Bradford with the BioRad Protein Assay reagent; BioRad, Hercules, CA) were used to determine CAT activity ...
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bioRxiv - Developmental Biology 2023Quote: ... SYBR green based real time qPCR reaction was prepared using So-Advanced Universal SYBR green Supermix reagents (Bio-Rad, catalog #172-5271) in a 96-well plate and analyzed using the Bio-Rad CFX Opus RT-qPCR equipment ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL of cDNA (10 ng of starting RNA) was amplified per reaction using the iTaq Universal SYBR Green Supermix (Bio-Rad, #1725124) and the Bio-Rad CFX96 qPCR system.
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bioRxiv - Genetics 2023Quote: ... Expression was measured with 20 ng of cDNA (calculated based on starting RNA quantity) in 10 μL reactions with SYBR Green (Bio-Rad, 1725124). Relative expression for knock-down was calculated using the Delta-Delta Ct method with Gapdh as the control reference ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time qPCR reactions were then run on a CFX96 Real-Time System C1000 Thermal Cycler (Bio-Rad, Marnes-la-Coquette, France). All samples were analyzed in triplicate and normalized for B2m and PGK1 expression ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories) and the TaqMan primers/probe mixtures (all from Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... 500 ng of total RNA was used in a 20 µL reaction volume with the Bio-Rad iScript cDNA synthesis kit (Bio-Rad, #1708890) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... All cDNA samples were diluted 1:5 and 1 μL was used in quantitative PCR (qPCR) reactions with iTaq Universal SYBR Green Supermix (Bio-Rad, #1725124), according to manufacturer’s instruction and using the Applied Biosystems QuantStudio 7 flex qPCR System ...