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Citations for Bio-Rad :
1651 - 1700 of 2175 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... The reaction for the multiplex real time PCRs contained 1× SYBR Green qPCR Master Mix (Bio-Rad, Hercules, CA, USA), 10 ng of each template ...
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bioRxiv - Microbiology 2021Quote: ... Each reaction was then loaded into a sample well of an eight-well disposable cartridge (DG8™; Bio-Rad Laboratories) along with 70 μl of droplet generation oil (Bio-Rad Laboratories) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The qPCR reactions were performed in triplicates on a CFX-96 thermocycler using SsoAdvanced Universal SYBR Green Supermix (BioRad, USA) using 5 ng of cDNA as a template in 10 µL reaction volume ...
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bioRxiv - Immunology 2020Quote: ... Droplet digital PCR: ddPCR reactions were performed on the QX200 Droplet Digital PCR system according to manufacturer’s instructions (Bio-Rad). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 μg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: For performing the digital droplet-PCR (ddPCR) we prepared the reaction mixture by combining a 2× ddPCR Mastermix (Bio-Rad), 20× primer ...
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bioRxiv - Immunology 2021Quote: ... each ddPCR reaction mixture was loaded into the sample well of an eight-channel disposable droplet generator cartridge (Bio-Rad). A volume of 60 μl of droplet generation oil (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... One hundred nanograms of cDNA were amplified in duplicates in each 40-cycle reaction using a CFX 384 Real Time PCR System (BioRad) with annealing temperature set at 60ºC ...
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bioRxiv - Neuroscience 2022Quote: ... was used for the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) by CFX96 Touch qPCR Detection System (Bio-Rad, 1855196). Data was analysed by CFX Manager software (Bio-Rad).
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bioRxiv - Developmental Biology 2020Quote: ... GEM-RT-PCR was performed in a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad; CT022510) to produce barcode cDNA using the following program ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs were diluted 1:10 and 1µl of diluted cDNA was used per qPCR reaction with iTaq Universal SYBR Green supermix (Bio-Rad) and the following pairs of primers ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription-quantitative Polymerase Chain Reaction (RT-qPCR) was performed using TaqMan probes with iTaq Universal Probe Supermix on a C1000 Thermal Cycler (BioRad). The following TaqMan (Applied Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... Detection of immunoreactive proteins was carried out using the enhanced chemiluminescence (ECL) reaction (SuperSignal ThermoScientific) and detected by the ChemiDoc Touch Imaging System (BioRad).
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bioRxiv - Biochemistry 2022Quote: ... Pelleted tau fibrils were then resuspended in 1/3-1/4 of the reaction volume and the concentration was determined by Coomassie band intensity measured against a standard and quantified in ImageLab (BioRad).
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bioRxiv - Genomics 2022Quote: qPCR was carried out in a 20 µL final volume reaction using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA), bespoke forward/reverse primers (0.3 μM/each ...
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bioRxiv - Genetics 2022Quote: ... followed by reverse transcription and sample indexing using a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (BioRad). After breaking the GEMs ...
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bioRxiv - Genetics 2022Quote: ... followed by linear PCR as described in the protocol using a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (BioRad). After breaking the GEMs ...
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bioRxiv - Microbiology 2022Quote: ... a 5X isothermal assembly reaction buffer (500 mM Tris-HCL pH 7.5, 50 mM MgCl2, 50 mM DTT (Bio-Rad), 31.25 mM PEG-8000 (Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... The real-time PCR was performed using a 20µl reaction volume on 96-well plates by using i-Taq Syber Green (Bio-Rad) according to the manufacturer’s instruction in the Bio-Rad CFX 96™ Real Time Detection System ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... Equal volumes of cDNA were used as template in a real-time quantitative PCR reaction using iQ SYBR Green Supermix (BioRad) on a CFX384 Touch Real-Time PCR System (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... Lysed samples were genotyped by performing a PCR reaction with Precision Melt Supermix for HRM analysis (Bio-Rad #172-5112) and stat1a- or stot1b-specific primers (Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... All steps were done manually using 8-strip PCR tubes and PCR reactions were carried out on a 96-plate thermal cycler (Biorad). Libraries were pooled and sequenced on a Nextseq550 sequencer (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction mixtures were held at 10°C until the PCR tubes were removed from the thermocycler (C1000 Touch, BioRad). After ...
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bioRxiv - Microbiology 2022Quote: ... competent cells were mixed with 500 ng of DNA in electroporation cuvettes (2 mm gap) and the reactions were electroporated (2.5 kV) using a MicroPulser electroporator (Bio-Rad). Electroporated cells were recovered in 1 mL LB (unless otherwise stated ...
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bioRxiv - Neuroscience 2022Quote: ... Pipetting steps were performed using the liquid-handling robots Dragonfly or Mosquito HV (SPT Labtech) using 384 well-plates and PCR reactions were carried out on a 384-plate Thermal Cycler (BioRad). Illumina sequencing of the resulting libraries was performed by Novogene (https://en.novogene.com/ ...
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bioRxiv - Neuroscience 2024Quote: ... 1000 ng of RNA was used for reverse transcription in a 6.5 µl reaction using the iTaq Universal SuperMix Two-step kit (Biorad, 1725130). 3 µl of the master mix for each probe and 2ul of the generated cDNA was pipetted into a 384-well plate ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time PCR reactions were carried out using SYBR green Master Mix on a CFX96 Real Time system (BIO-RAD). Primer sequences are provided in the following table ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 μg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... A total reaction mixture with a volume of 20 µl was amplified in a 96-well PCR plate (Bio-Rad). The primer sets used are listed in table supplement 1.
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bioRxiv - Cancer Biology 2024Quote: ... A total of 1µg of RNA in 20µl reaction volume was reverse transcribed using the iScript Reverse Transcription Supermix for RT-PCR (Bio-Rad). RT-qPCR was performed for TNFA ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription and library preparation were performed on C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad). Amplified cDNA and final libraries were evaluated on an Agilent BioAnalyzer using a High Sensitivity DNA Kit (Agilent Technologies) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The reaction was kept at 37°C and recorded using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). To prevent evaporation the plate was sealed with a transparent sticker ...
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bioRxiv - Immunology 2022Quote: ... followed by reverse transcription and sample indexing using a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (BioRad). After breaking the GEMs ...
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bioRxiv - Molecular Biology 2023Quote: The mRNA levels were assessed by real-time quantitative polymerase chain reaction (RT-qPCR) with Bio-Rad CFX96 (Bio-Rad), and a 20 μL of reaction ...
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bioRxiv - Microbiology 2022Quote: ... Several 10-fold dilutions of each purified DNA were prepared in nuclease-free H2O and added to a reaction mixture containing ddPCR EvaGreen Supermix (Biorad). Forward (5′-GAATACATTCACATTGACCAATCAGAA-3′ ...
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bioRxiv - Plant Biology 2023Quote: RT-qPCR reaction mixtures were set up according to the manufacturer’s instructions (Blue ŚGreen, Biozym) and reactions were carried out on a CFX96TM Touch thermocycler (Bio-Rad). As a control for contamination with genomic DNA ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed with the SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) by a Bio-Rad CFX96™ Real-Time PCR System according to the manufacturer’s manual ...
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bioRxiv - Immunology 2023Quote: ... The quantitative RT-PCR (RT-qPCR) amplification reactions were conducted using IQ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) and ran in the CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... following the users’ manual and the reaction was run in CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) under the setup with an initial denaturation at 95 C for 3 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR reactions were performed in technical triplicates per each input cDNA using iQ SYBR® Green Supermix (Bio-Rad). Primers for all targets were as previously described ...
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bioRxiv - Microbiology 2023Quote: ... Recovered DNA suspended in molecular grade water was used for Sybr Green reactions (1 µL) on a BioRad CFX384 (BioRad). ChIP-qPCR primers (50-150 bp ...
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bioRxiv - Developmental Biology 2023Quote: ... we ran real-time quantitative polymerase chain reactions (qPCR) on a BioRad CFX96 Touch Real-Time PCR System (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: 500-1000 ng of RNA were used for the retrotranscription reaction using iScript cDNA Synthesis Kit (BioRad, CA, USA, #1708890). Expression values were determined by q-PCR using Sybr Green (iTaq SYBR Green Supermix ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR duplicates were carried out using 2 µL of diluted cDNA (1 into 100) in a 20 µL-reaction using Sso Fast EvaGreen reagent (BioRad) following supplier’s instructions on a StepOne Plus™ Real-Time PCR system (Applied BioSystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... held at 4 °C) was performed in a C1000 Touch Thermal Cycler with 96-Deep Well Reaction Module (Bio-Rad). After RT reaction ...
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bioRxiv - Genetics 2023Quote: ... The 20 μl copy number reaction mix consisted of 10 μl of 2x ddPCR SuperMix for Probes (Bio-Rad Laboratories), 1 μl of the Copy Number target assay (labeled with FAM) ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR reactions were prepared from cDNA at 1:5 dilution with SYBR Green iTaq Universal SYBR Green Supermix (Biorad, 1725120) and qPCR primers at 10 µM in a total volume of 10 µL in a ≥ 40-cycle and melt curve reaction cycler (Biorad ...
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bioRxiv - Molecular Biology 2023Quote: ... and qPCR primers at 10 µM in a total volume of 10 µL in a ≥ 40-cycle and melt curve reaction cycler (Biorad, Base #CT009383 ...
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bioRxiv - Cell Biology 2023Quote: ... One μg of RNA was used to perform the reverse transcriptase reaction with iScript TM cDNA Synthesis Kit (Bio-Rad). Changes in mRNA levels of the target genes were determined by relative RT-qPCR with a CFX96TM Real‒Time PCR Detection System (Bio-Rad ...
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bioRxiv - Systems Biology 2023Quote: ... and heated at 95°C for 60 minutes in a thermal cycler (Bio-Rad S1000 with 384-well reaction module) at a constant lid temperature of 110°C ...