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Citations for Bio-Rad :
1601 - 1650 of 2175 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... followed by reverse transcription and sample indexing using a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (BioRad). After breaking the GEMs ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative real-time PCR reactions were performed using Roche LightCycler 480 system with a SsoFast EvaGreen Supermix (#1725201, Bio-Rad). Each sample was run in triplicate ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized using 600 ng input total RNA per 40 ¼l reaction with the iScript cDNA Synthesis Kit (BioRad), cycling for 5 min at 25°C ...
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bioRxiv - Plant Biology 2022Quote: ... The reaction was carried out in a thermal cycler CFX384™ Touch Real-Time PCR (Bio-Rad, Hercules, CA, USA). The results for maritime pine were normalized using a saposin-like aspartyl protease (unigene1135 ...
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bioRxiv - Neuroscience 2021Quote: ... Synthesis of cDNA was performed in duplicate from 500ng RNA in 10μL reactions using the iScript Advanced cDNA Synthesis kit (Bio-Rad). Reactions were diluted 1:5 in water ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCR reactions were run with Sso Advanced Universal SYBR Green Supermix on a CFX384 RT-qPCR system (BioRad, Hercules, CA).
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bioRxiv - Developmental Biology 2020Quote: ... Protein bands were visualised by triggering the HRP-catalysed chemiluminescent reaction catalysed through addition of the “Clarity” (TM) Western ECL Substrate (BioRad) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Polymerase Chain Reaction (PCR) was carried out in 0.2 mL volume PCR in a thermal cycler (T100 Thermal cycler, Biorad, USA). HF Phusion Master mix (M0531S ...
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bioRxiv - Microbiology 2020Quote: ... Annealing temperatures were optimized for each reaction using gradient PCR with ddPCR Supermix for Probes (Bio-Rad, Mississauga, ON, Canada) using 900 nM each primer and 250 nM of hydrolysis probe in a 20 μl reaction volume ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA was diluted to 10 ng/µland 2 µl was used in each RT-qPCR reaction with 10 µl iTAQ Universal SYBR green supermix (BioRad) and 400 nM primers ...
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bioRxiv - Genomics 2020Quote: ... Linear amplification and droplet-based indexing were performed as described in the 10x ATAC protocol on a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (BioRad). After linear PCR ...
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bioRxiv - Plant Biology 2020Quote: ... was used at a dilution of 1:2,000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:2,000) followed by a chemiluminescence reaction (Clarity Western ECL Substrate; Bio-rad). For the SS-TOM lines analysis ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... The 20 μL reaction mix contained 5 μL sample or standard DNA together with 10 μL 2x SsoAdvanced Universal Probes Supermix (Biorad), 1 μL of each primer (5 μM stock solution) ...
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bioRxiv - Bioengineering 2021Quote: ... was used to prepare the qPCR reaction that was run on a CFX384 Real Time Thermal Cycler (BioRad, Hercules, CA). Additional AAV was tested for eGFP transgene expression using an in vitro assay ...
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bioRxiv - Bioengineering 2020Quote: ... Fifty nanograms of cDNA were amplified in duplicates in each 40-cycle reaction using a CFX 384 Real Time PCR System (BioRad) with annealing temperature set at 60°C ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were incubated for 15 min at 30 °C and then applied to a Bio-Gel A-1.5m (Bio-Rad) resin packed in a 0.7×15cm Flex-Column (Kimble) ...
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bioRxiv - Cancer Biology 2020Quote: ... Reverse transcription and library preparation was done on C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad). Amplified cDNA and final libraries were evaluated on an Agilent BioAnalyzer using a High Sensitivity DNA Kit (Agilent Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using iTaq Universal SYBR Green qPCR Master Mix (Biorad, Hercules, CA), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions were performed in duplicate using the iCycler iQ Thermal Cycler with a 96 x 0.2 ml reaction module and iCycler iQ software (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: A 5X isothermal assembly reaction buffer (500 mM Tris-HCL pH 7.5, 50 mM MgCl2, 50 mM DTT (Bio-Rad), 31.25 mM PEG-8000 (Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... 15 μL of each reaction was immediately loaded onto an 8% TBE gel (Acrylamide/Bis solution 37.5:1, Bio-rad) supplemented with 2 mM MgCl2 ...
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bioRxiv - Microbiology 2021Quote: Isothermal assembly reaction buffer (5×) (500 mM Tris-HCl [pH 7.5], 50 mM MgCl2, 50 mM dithiothreitol [DTT] [Bio-Rad] ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 μl of ChIP-ed or input (diluted 30-fold to bring it to 1%) DNA was used in 20 μl total volume reaction using SYBR green master mix (BioRad) and 0.4 μM of each primer ...
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bioRxiv - Microbiology 2021Quote: ... Signals were detected by autoradiography using HRP reaction (WesternBright Quantum HRP Substrate for CCD, Diagomics) on a ChemiDoc Touch Imaging System (BioRad).
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bioRxiv - Microbiology 2021Quote: Digital PCRs were carried out as duplex reactions and analyzed with the QX100 Droplet Digital PCR System (Bio-Rad, California).9,11 For human samples ...
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bioRxiv - Molecular Biology 2020Quote: ... Each reaction contained 3 technical replicates and were carried out using a CFX384 TouchTM Real-Time PCR Detection System (BioRad Laboratories Ltd. ...
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bioRxiv - Cancer Biology 2020Quote: ... Target sequences in cDNA library were amplified in 10 μl qPCR reaction (5 μl SYBR Green supermix (Bio-Rad #1725121), 0.675 μl 2.5 μM primer mix and 0.45 μl diluted cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... Each reaction was stopped by addition of SDS-PAGE loading buffer and run on pre-maid SDS-PAGE gels (Biorad). The autophosphorylation of hIRE1 KR was confirmed by western blot using a pSer/24 phospho-specific antibody (Genentech ...
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bioRxiv - Biochemistry 2022Quote: ... The qPCR reactions were carried out in a 96-well plate in CFX96 Real-time PCR Detection System (Bio-Rad). The primers were listed in Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... PCR reaction and amplicon detection were performed by the iCycler real-time PCR system (CFX Connect RT-PCR System, Bio-Rad). Quantification of expression was normalized according to the relative levels of cDNA of the house-keeping genes RNA polymerase II (RNAPII ...
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bioRxiv - Bioengineering 2022Quote: 2ul of each CUT&RUN sample was run in 20ul reaction following Bio-Rad protocols targeting Adipoq (Bio-Rad, 10025636). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... the enzymes were buffer exchanged into their respective reaction buffers above using a Micro Bio-Spin 6 size exclusion spin column (Biorad) to remove glycerol and other ion suppressing constituents ...
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bioRxiv - Developmental Biology 2022Quote: ... 1ug RNA was in a 20ul reaction volume to generate cDNA using iScript™ cDNA Synthesis Kit (Bio-Rad, 1708891). Typically ...
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bioRxiv - Microbiology 2022Quote: ... and cDNA (1.0 μL) of cell or EV were prepared in 20 μL reaction and run on CFX96 qPCR machine (Bio-Rad). Gene expression level were first normalized to housekeeping gene GAPDH and then calculated with ΔΔCt method ...
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bioRxiv - Genetics 2022Quote: ... we made 21 µL of reaction mix for each sample according to the manufacturer’s directions (Bio-Rad Laboratories, Hercules, CA). Each reaction consisted of 2× ddPCR Supermix for Probes ...
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bioRxiv - Cell Biology 2022Quote: ... All quantitative PCR (qPCR) reactions were performed in triplicate on a CFX-384 Real-Time PCR detection system (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... was used for RT-qPCR using three technical replicates per reaction in a CFX96 Touch Real-Time PCR Detection System (Biorad). Primers are described in Table S4 ...
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bioRxiv - Neuroscience 2020Quote: ... with each reaction performed at a 20 μl sample volume in an iCycler iQ PCR 96-well Plate (Bio-Rad) sealed with Microseal optical sealing tape (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were performed with SYBR Green Master Mix (Thermo cat. 4309155) using three technical replicates per reaction in a CFX96 Touch Real-Time PCR Detection System (Biorad). TUBULIN1 was used as a normalizer for ChIP-qPCR experiments and PP2AA3 for RT-qPCR (Czechowski et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time quantitative PCR (qPCR) reactions were performed in duplicate using the CFX-96 Real Time PCR system (Bio-Rad). Quantitative PCR primers were designed using Primer3 software and synthesized by Yale School of Medicine Oligo Synthesis facility ...
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bioRxiv - Genetics 2022Quote: ... and a 2-μl aliquot was used in a 12-μl PCR reaction with SsoAdvanced Universal Probes Supermix (Bio-Rad) and specific TaqMan primer/probe assays for Gaa (Taqman assay #Mm00484581_m1 ...
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bioRxiv - Physiology 2022Quote: ... All qRT-PCR reactions were done in triplicate on a 96-well plate using a CFX Connect qRT-PCR machine (BioRad). Each well contained a 10 μL reaction ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... Quantitative PCR reactions were carried out in triplicate on specific genomic regions using SYBR Green Supermix (Bio-Rad, Hercules, CA). The resulting signals were normalized for primer efficiency by carrying out qPCR for each primer pair using Input DNA ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction for the multiplex real time PCRs contained 1× SYBR Green qPCR Master Mix (Bio-Rad, Hercules, CA, USA), 10 ng of each template ...
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bioRxiv - Biochemistry 2022Quote: ... The PCR reaction was carried out in 10 μl volume with 5 μl of 2X iTaq Universal Sybergreen Supermix (BioRad), 500 nM of forward and reverse primers (for promoter ...
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bioRxiv - Bioengineering 2020Quote: A reverse transcription polymerase chain reaction (RT-PCR) was carried out on the RNA using the mix iScript Reverse Transcription Supermix (Biorad) and the following thermocycler program ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were then PCR amplified: 50 μl reactions (25 μl SsoFast Evagreen supermix with low ROX (Bio-Rad, Hercules, CA), 0.6 μl 25 μM custom Nextera PCR primer 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription and library preparation were performed on C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad). Amplified cDNA and final libraries were evaluated on an Agilent BioAnalyzer using a High Sensitivity DNA Kit (Agilent Technologies) ...
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bioRxiv - Immunology 2020Quote: ... Purified libraries were diluted 1:100 and quantified again by qPCR (Two 25uL reactions, 2x iQ SYBR SUPERMix (Bio-Rad, with Read 1 (5’-TATGGTAATTGT GTGYCAGCMGCCGCGGTAA-3’) ...
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bioRxiv - Molecular Biology 2020Quote: ... Droplets were generated using 20 uL reaction mixture and 70 uL oil with the QX200 Droplet Generator (Bio-Rad Laboratories). Droplets were transferred to a 96-well PCR plate ...