Labshake search
Citations for Bio-Rad :
8551 - 8600 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... Protein samples were separated by gel electrophoresis using 13% Tris-Tricine polyacrylamide gels and either transferred to a 0.45-μm nitrocellulose membrane (Bio-Rad Laboratories, Hercules CA) or stained with Coomassie blue (Bio-Rad Laboratories ...
-
bioRxiv - Immunology 2024Quote: ... cell lysates were separated by SDS-polyacrylamide gel electrophoresis followed by Western Blot transfer to nitrocellulose membranes (0.2 µm, Bio-Rad, Hercules, CA, USA) using the Trans-Blot Turbo Transfer System (Bio-Rad ...
-
bioRxiv - Microbiology 2024Quote: ... incubated for 5 min at 95 °C and 15 μL of each sample were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a nitrocellulose membrane (Bio-Rad, 0.45 µm). For this ...
-
bioRxiv - Neuroscience 2024Quote: ... The transfer was carried out to 0.45 μm nitrocellulose membranes for 20 min per membrane at constant 25 mA in a semi-dry transfer chamber Trans-Blot® Turbo™ Transfer Solution from Bio-Rad (Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2024Quote: ... Ten micrograms of total proteins were separated on 4-15% pre-cast SDS-PAGE gels and then transferred to PVDF membranes using a semi-dry Turbo transfer system (Bio-Rad; Hercules, CA). Protein blots were blocked in 5% blotting-grade blocker milk for 1 hour at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... SDS-polyacrylamide gels and transferred onto nitrocellulose membranes (AmershamTM ProtranTM Premium 0.45 µm NC, Cytiva AmershamTM) using the Trans-Blot® Turbo™ System (Bio-Rad Laboratories).
-
bioRxiv - Molecular Biology 2024Quote: ... with equal protein loading and separated by SDS-PAGE gel electrophoresis prior to transfer to polyvinylidene difluoride membranes (Trans-Blot® Turbo™ Transfer System, Biorad, #1704274). Membranes were subjected to blocking and antibody incubation as previously described.37 For GABARAPL1 blots ...
-
bioRxiv - Neuroscience 2024Quote: 20ug from RIPA lysate was loaded onto PVDF membranes by gravity filtration using a Bio-dot microfiltration apparatus (Bio-Rad Laboratories, Hercules, CA) for amyloid peptides and oxidative damage markers ...
-
bioRxiv - Genetics 2024Quote: ... 20-40µg of total proteins were separated with SDS-PAGE in 4-15% polyacrylamide gel and then transferred to polyvinylidenedifluoride (PVDF) membranes (Bio-Rad, Hercules, CA). The membranes were blocked with 5% non-fat milk and immunoblotted with primary antibodies against CFTR (769 from the UNC distribution program ...
-
bioRxiv - Genomics 2024Quote: ... Equal amounts of protein were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to NC membranes (Bio-Rad, no. 1704158). 4× Laemmli Sample Buffer (Bio-Rad ...
-
bioRxiv - Biochemistry 2024Quote: ... We then transferred the proteins to a 0.2 µm nitrocellulose membrane (Amersham, Cat. No. GE10600001) using a Criterion blotter (Bio-Rad, Cat. No. 1704070) in Towbin transfer buffer at 100V for 20 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... After being dried at room temperature (RT) for 1 h and gDNA was crosslinked to the membrane using GS Gene Linker (Bio-Rad, Hercules, CA), then blocked with 5% nonfat milk for 1 h at RT ...
-
bioRxiv - Cell Biology 2024Quote: Protein extracts were resolved by SDS-PAGE, then transferred to a nitrocellulose membrane (Cytivia Amersham; Amersham, Buckinghamshire, UK) using a wet transfer system (BioRad; Hercules, CA, USA) at 100V for 60 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... Transfer to nitrocellulose membrane was carried out at 25 V and 1 A for 30 min in a Trans-Blot Turbo system (Bio-Rad, Hercules, USA). Membranes were blocked in 5% milk powder/PBS for 1 h ...
-
bioRxiv - Physiology 2020Quote: ... cDNA was synthesized using a reverse transcription kit (iScript cDNA synthesis kit; Bio-Rad, Hercules, CA) from 1 μg of total RNA ...
-
bioRxiv - Microbiology 2024Quote: ... and converted to cDNA using the iScript cDNA synthesis kit (iScript cDNA synthesis kit; Bio-Rad). Real-time quantitative PCR (qPCR ...
-
bioRxiv - Developmental Biology 2021Quote: ... An iScript cDNA synthesis kit (BioRad) was used on 500ng of RNA for cDNA synthesis ...
-
bioRxiv - Developmental Biology 2021Quote: ... An iScript cDNA synthesis kit (BioRad) was used on 500ng of RNA for cDNA synthesis ...
-
bioRxiv - Molecular Biology 2021Quote: ... iScript cDNA synthesis Kit (Biorad, 1708891) was used to transcribe the isolated RNA to first strand cDNA ...
-
bioRxiv - Molecular Biology 2020Quote: ... using the iScript kit (Bio-Rad). qPCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher ...
-
bioRxiv - Cell Biology 2021Quote: ... and a colorimetric detection kit (BioRad).
-
bioRxiv - Microbiology 2021Quote: ... AP conjugate substrate kit (Bio-Rad) was used to develop spots ...
-
bioRxiv - Cancer Biology 2022Quote: ... The SsoFast EvaGreen Supermix kit (BioRad) was used for qRT-PCR using recommended cycling conditions ...
-
bioRxiv - Neuroscience 2022Quote: ... The iScriptTM cDNA Synthesis Kit (BioRad) was used to transcribe 500ng of total head RNA according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... iScript cDNA synthesis kit (Bio-Rad) to reverse transcribe total RNA into cDNA ...
-
bioRxiv - Cell Biology 2020Quote: ... The iScript cDNA Synthesis Kit (BioRad) was used to generate cDNA from equal amounts of total RNA (1mg ...
-
bioRxiv - Cell Biology 2021Quote: ... or iScript cDNA synthesis kit (BioRad). cDNA was diluted five-fold and quantitative PCR (RT-qPCR ...
-
bioRxiv - Microbiology 2021Quote: ... synthesis kit (Bio-Rad, Hercules, CA). CoV-2 S and N transcripts and 18s rRNA were quantified by ddPCR with specific primers (CoV-2 RNA Leader ...
-
bioRxiv - Neuroscience 2022Quote: ... or iScript cDNA synthesis kit (Biorad) for mRNA detection ...
-
bioRxiv - Cell Biology 2024Quote: ... SYBR Green Master mix kit (BioRad) and Stratagene Mx3000p detection system were used to perform quantitative analysis of gene expression ...
-
bioRxiv - Microbiology 2023Quote: ... 37-Plex kit (171AL001M; Bio-Rad) Bio-Plex Human Chemokine Panel ...
-
bioRxiv - Biochemistry 2023Quote: ... and HRP substrate kit (Bio-Rad) were added in sequence ...
-
bioRxiv - Immunology 2022Quote: ... or iScript cDNA synthesis kit (BioRad) according to the manufacturer guidelines ...
-
bioRxiv - Biochemistry 2022Quote: ... using the ChemiDoc kit (BIO-RAD) and digitalized with the Chemi-Doc XRS System (BIO-RAD).
-
bioRxiv - Plant Biology 2023Quote: ... iScript cDNA synthesis kit (Bio-Rad) was used for cDNA synthesis ...
-
bioRxiv - Plant Biology 2024Quote: ... iScript cDNA synthesis kit (Bio-Rad) was employed to reverse-transcribe 2 µg of the DNase I-treated RNA ...
-
bioRxiv - Microbiology 2024Quote: ... or Clarity chemiluminescence kit (Bio-Rad) according the manufacturer’s protocol and imaged using the iBright 1500 Imager (Invitrogen).
-
bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared at 12 000 rpm for 10 min at 4°C and protein concentration adjusted with Bradford assay (BioRad). The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega ...
-
bioRxiv - Molecular Biology 2020Quote: ... Gene expression was detected using SybrGreen RT-qPCR expression assays in a 96-wells plate format as described by the manufacturer using MyIQ real-time PCR cycler (BioRad); cDNA templates were used at a 1:40 dilution ...
-
bioRxiv - Developmental Biology 2021Quote: ... The supernatant was collected for BCA assay and analyzed by SDS-PAGE using Mini-Protean® TGXTM 4– 15% (BioRad) and transferred to nitrocellulose membranes ...
-
bioRxiv - Cancer Biology 2022Quote: ... Samples were then sonicated and their protein content was quantified using the Bradford protein assay (Bio-Rad, Hercules, CA, USA). For each sample ...
-
bioRxiv - Molecular Biology 2021Quote: ... for 24 hours before being lysed in RIPA buffer containing protease inhibitors and protein concentration assessed using RcDc assay (BioRad). In some experiments cells were treated with 2μM of VX-445 (Selleckchem) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The supernatant from the cell lysate was collected by centrifuging the cell lysate at 10,000 g for 10 min at 4°C and further diluted in lysis buffer to 8.0 ug/ul using a modified Bradford protein quantification assay (Bio-Rad), flash-frozen in liquid N2 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... 80μg of proteins per sample was labelled and protein estimation was done using RC-DC protein assay from Bio-Rad.
-
bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in 1X PBS and mechanically lysed by sonication before protein standardized by DC assay (BIO-RAD) and mixed with Western blot sample loading buffer (2X loading buffer (LI-COR) ...
-
bioRxiv - Immunology 2021Quote: Multiplexed cytokine assays were performed according to the manufacturer instructions by using a Bio-Plex Pro Human cytokine screening panel (Biorad) containing 48 human cytokines ...
-
bioRxiv - Biochemistry 2020Quote: ... Tissue lysates for Western blot were prepared as previously described [11] and sample protein content was determined by Bradford assay (BioRad). Lysates were loaded in 10% polyacrylamide gels and transferred to Immobilon membranes (Millipore ...
-
bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared of insoluble debris by centrifugation prior to determination of protein concentration by BCA assay (BioRad, CA, USA). Lysates were electrophoresed through either 4-12% NuPAGE or 8% E-PAGE gels (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... The concentration was then measured by DC assay and 20 μg of protein was resolved on an SDS page gel (gradient 4%-20%, BioRad) and transferred to a nitrocellulose membrane ...
-
bioRxiv - Cancer Biology 2020Quote: Protein lysates (see “RAS activity assays”) were mixed with 4×Laemmli buffer (Cat.#161-0747; Bio-Rad Laboratories, Hercules, US) containing fresh 200 mM DL-Dithiothreitol (Cat.#3483-12-3 ...