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Citations for Bio-Rad :
8751 - 8800 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... here the proteins were separated on SDS-gels and transferred onto a nitrocellulose membrane by the semi-dry Transblot Turbo Transfer System (Bio-Rad, Hercules, CA, USA). Primary antibodies were obtained from Agrisera (Vännäs ...
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bioRxiv - Physiology 2023Quote: ... Fifty micrograms of protein were separated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using the Trans-Blot Turbo transfer pack (Bio-Rad Laboratories, Hercules, CA). Membranes were blocked for 1 h at room temperature in Tris buffer saline 1% Tween-20 (TBS-T) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were separated using 10% SDS page and blotted on a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories, Inc., Austria). The membrane was probed with the anti pY-4G10 (mouse ...
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bioRxiv - Cell Biology 2023Quote: ... Separated proteins were transferred from the gel onto the membrane by semi-dry blotting using the Trans-Blot Turbo™ Transfer System (Bio-Rad, Munich, Germany) with a discontinuous buffer system consisting of one cathode buffer and two anode buffers ...
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bioRxiv - Cell Biology 2022Quote: ... 30 μl were loaded and resolved on 6% sodium dodecyl sulfate (SDS) gels and transferred onto nitrocellulose membranes with Mini-PROTEAN III and Mini Trans-Blot cells (Bio-Rad, Hercules, CA, USA), respectively ...
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bioRxiv - Genetics 2022Quote: ... proteins were transferred to a pre-activated PVDF membrane using the Trans-Blot®Turbo™ Transfer System and visualized using ECL (Bio-Rad Chemidoc). Antibodies used in this study are ...
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bioRxiv - Neuroscience 2022Quote: ... Solid phase specificity was confirmed with dot blots of serial antigen dilutions (2 μL) applied to nitrocellulose membrane (Bio-Rad 0.45 µm; Fig. 1b). Membranes were allowed to air dry (1 h ...
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bioRxiv - Neuroscience 2023Quote: ... A 5 μL aliquot of the aSyn protein not incubated or incubated for different periods under aggregating conditions was transferred to a nitrocellulose membrane (Bio-rad®; 0.45 μm). The sample was then blocked with OdysseyTM blocking buffer for 60 min at 22° C ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of proteins (20 μg) were separated by SDS-PAGE and then transferred onto nitrocellulose membranes (ref 1704271, Bio-Rad, Hercules, California, USA). Membranes were incubated in blocking buffer ...
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bioRxiv - Biophysics 2023Quote: ... then transferred onto PVDF membrane (Hybond-LFP, Amersham BioSciences, Little Chalfont, Buckinghamshire, United Kingdom) by using a semi-dry blotter (Trans-Blot Cell, Bio-Rad, Hercules, CA, USA). To differentiate the truncated product from T2 ...
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bioRxiv - Bioengineering 2023Quote: ... samples were run on a SDS-PAGE and transferred onto a nitrocellulose membrane using a semi-dry transfer protocol (Bio-Rad laboratories, Hercules, California). Membranes were washed and incubated with primary antibody (p-IRF3 ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were then separated by electrophoresis on a 10% SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Cat. No. 1620177). The membrane was blocked with 5% bovine serum albumin (B.S.A. ...
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bioRxiv - Microbiology 2024Quote: ... resolved on the 10% SDS PAGE and transferred to nitrocellulose membrane (Trans-Blot Turbo Midi Nitrocellulose Transfer Pack, Bio-Rad, 0.2 µm pore size) with the use of a Trans-Blot Turbo Transfer Starter System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: 20 µg of proteins were separated on a 10% polyacrylamide gel and transferred on nitrocellulose membranes (Amersham™ Protran®; #106000004) using Trans-Blot® Turbo™ (BioRad). Blocking was performed through a 1h incubation in 5% low fat milk in TBST ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked for 1 hr at room temperature in gentle shaking with blocking buffer (0.1% Casein [BioRad 1610782] in Tris Buffered Saline [TBS]). Membranes were incubated overnight at 4C with gentle shaking with the primary antibody in blocking buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... in TG-SDS running buffer (Bio-Lab ltd., Jerusalem, Israel) and blotted onto PVDF membranes (Trans-Blot Turbo Transfer Pack; Bio-Rad Hercules, CA, USA). The membrane was blocked using 5% (w/v ...
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Gain-of-function study reveals the pleiotropic roles of serine protease HtrA in Borrelia burgdorferibioRxiv - Microbiology 2024Quote: ... Approximately 10-20 μg of bacterial whole cell lysates was loaded into each lane of 12% SDS-PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories, Hercules, CA). The immunoblots were probed with antibodies against B ...
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred to nitrocellulose membranes at 90 V for 90-100 minutes by a Mini Trans- Blot cell (Bio-Rad Laboratories, Feldkirchen, Germany). Membranes were blocked with TBST-5% dry milk at RT for 1 hour then incubated overnight with primary antibodies ...
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bioRxiv - Plant Biology 2024Quote: RNA samples resolved in denaturing polyacrylamide gels were transferred to positively charged nylon membranes (Cytiva, Hybond-N+, catalog no. 45-000-850) using a semi-dry Trans-Blot Transfer System (Bio-Rad, catalog No. 1703940) in 0.5X TBE at constant 20 V for 45 min ...
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bioRxiv - Molecular Biology 2024Quote: ... using a Laemmli buffer system and then semidry transferred to nitrocellulose membranes by a Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA). The membranes were blocked with either 5% bovine serum albumin in PBS or 5% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... The collected protein samples were electrophoresed on 10% SDS-polyacrylamide gel (SDS-PAGE) and transferred to the polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Cat. No. 1620177). After blocking with 5% bovine serum albumin (BSA ...
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bioRxiv - Physiology 2024Quote: ... 10-20 µg tissue lysates were separated by 8% gel in SDS-PAGE and transferred to nitrocellulose membranes (Trans-Blot, Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% nonfat dried milk or 3% BSA (for phosphorylated proteins ...
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bioRxiv - Developmental Biology 2024Quote: ... under reducing conditions using 2-ME and transferred onto polyvinylidene difluoride (PVDF) membrane using the Trans Blot Turbo system (Bio-Rad, Hercules, CA, USA). After blocking with 10% skim milk (Becton Dickinson ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were loaded onto 4-12% Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels for 60-90 minutes in SDS-PAGE running buffer and transferred to Polyvinylidene difluoride (PVDF) membranes (Bio-Rad, Hercules, CA, USA). Membranes were stained with Ponceau S to verify the transfer of proteins ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to an Amersham Protran Premium 0.2 NC nitrocellulose Western blotting membrane (Cytiva, Marlborough, MA, USA) using a Trans-Blot® TurboTM Transfer System (Bio-Rad Laboratories, Hercules, USA) and a gradient transfer buffer (0.3 M Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 30-40 μg of proteins from the lysates were fractionated on SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad Laboratories, CA, USA). Membranes were probed with rabbit anti-HIF2α (ab243861 ...
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bioRxiv - Cell Biology 2024Quote: ... EZ-Western Lumi Pico or Femto (DOGEN, Seoul, Korea) was used to treat the membrane and was visualized using a ChemiDoc XRS+ system (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Immunology 2023Quote: ... The second kit used was the Bio-Plex Pro Serology Reagent Kit (Bio-Rad, Cat No. 12014777), with human IgA positive and negative controls (Bio-Rad ...
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bioRxiv - Synthetic Biology 2021Quote: ... The culture supernatant was obtained by centrifugation and used with the Bio-Plex Pro Human Cytokine 8-plex Assay (Bio-rad) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor samples were homogenized in RIPA buffer (Thermo, #89901) followed by protein concentration measurement using DC Protein Assay (Bio-Rad, #5000112). Tumor lysates were immunoblotted as above ...
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bioRxiv - Cell Biology 2020Quote: ... Concentration of total cellular protein was measured according to the DC™ protein assay according to the manufacturer’s instruction (BIO-RAD).
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bioRxiv - Microbiology 2020Quote: ... and the total protein concentration of the samples was determined by Bradford assay70 with Protein Assay Dye Reagent Concentrate (BIO-RAD). Samples were normalized by total protein content and run in a 10% SDS-PAGE gel for protein separation ...
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bioRxiv - Cell Biology 2020Quote: ... EVs were characterized based on particle size and number using nanoparticle tracking analysis (NS300, Nanosight) and protein concentration (DC protein assay, Bio-Rad).
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bioRxiv - Genetics 2021Quote: ... the ratio of droplets positive for these sequences versus droplets positive to a reference gene (RPP30 PrimePCR™ Probe Assay, BioRad) was used as a readout ...
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bioRxiv - Developmental Biology 2021Quote: ... Aliquots of larval homogenate were removed prior heat-treatment to measure soluble protein using a Bradford assay (Bio-Rad 500-0006) with BSA standard curves.
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bioRxiv - Biochemistry 2020Quote: ... The GlcA-T activity was assessed by subtracting the background observed in the negative control sample without donor sugar and normalized against the amount of protein measured using the DC Protein Assay (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Expression of Ace2 and the reference gene Gapdh were measured by Reverse Transcriptase droplet digital PCR (Prime ddPCR assays, QX200, Bio-Rad). Gene expression was quantified as the transcript ratio of Ace2 to Gapdh.
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bioRxiv - Molecular Biology 2020Quote: ... Extracts were then incubated on ice for 10 min before protein concentration was calculated by Bradford assay (Bio-Rad, 500-0006). Extracts were then mixed with Laemmli buffer and boiled at 95°C for 5 minutes before loading on SDS-PAGE gels.
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... supplemented with 1 mM DTT for reduction of disulfide bonds and protein concentration was determined by Quick-start Bradford assay (Bio-Rad). Proteins were alkylated with 5.5 mM chloracetamide (45 min ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were cleared by centrifugation at 16000xg for 5 min at 4°C and the total protein concentration in the lysates was measured by Bradford protein assay (Bio-Rad). For western blot analysis 25 µg of each lysate was boiled for 5 min at 98°C (for the detection of LC3B and ATG9A samples were heated at 60°C for 10 min ...
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bioRxiv - Immunology 2021Quote: ... Samples were centrifuged and the protein content of the supernatant was measured using the Protein Assay Dye Reagent (Biorad, #500-0006). Immunoblotting was performed using from 10 to 50 μg of protein lysate ...
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bioRxiv - Neuroscience 2021Quote: ... The cAMP levels were normalized to the total protein concentration in the supernatant determined by Bradford Assay using BSA standard curve (Bio-Rad). The final cAMP concentration is expressed as pmol cAMP per mg of total protein ...
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bioRxiv - Neuroscience 2021Quote: ... quantified by micro-BCA assay (Pierce) in duplicate extracts) on 4-20% gradient Bis-Tris polyacrylamide stain-free pre-cast gels (Bio-Rad) in denaturing conditions ...
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bioRxiv - Neuroscience 2021Quote: ... and sonicated (twice; 70 % power for 30 sec, Sonopuls, Bandelin) prior to protein concentration determination with the DC Protein Assay (Bio-Rad) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... Mutations in ESR1 (hotspots D538 and Y537) and PIK3CA (hotspots E453 and H1047) were detected by either ddPCR assay using the QX200 ddPCR System (Bio-Rad) or through the Guardant360™ high sensitivity next-generation sequencing platform (Guardant Health ...
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bioRxiv - Cancer Biology 2020Quote: ... and growth factors in culture supernatants of control or R1881 treated LNCaP cells was determined using the Bioplex Pro Human Cytokine 17-plex assay system (Bio-Rad) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Purified proteins were then dialyzed against Lysis Buffer to remove GSH and quantified using a Bradford assay per the manufacturer instructions (BioRad, 5000006) prior to being stored at −80oC ...
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bioRxiv - Biochemistry 2021Quote: ... and 5 mM β-mercaptoethanol) until the washes indicate a protein concentration of less than 0.1 mg/mL by Bradford assay (Bio-rad). The protein was eluted with elution buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... cAMP content in TCA extracts was determined by radioimmunoassay (RIA) (83) and normalized to protein [(determined using a dyebinding protein assay (Bio-Rad)] ...
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bioRxiv - Microbiology 2021Quote: ... ddPCR was performed according to manufacturer’s instructions for the 2019-nCoV CDC ddPCR Triplex Probe Assay (Bio-Rad, Hercules, California, USA). PCR reaction mixtures were as follows ...