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Citations for Bio-Rad :
8801 - 8850 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 2 μl of brain homogenate from each sample was analyzed for protein concentration using BioRad protein assay reagent (BioRad, Hercules, CA). 1 μl of 0.2 M perchloric acid per sample was added to the remaining homogenate and was centrifuged at 10,000 rpm for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Triton-insoluble material was then pelleted at 15890 x g for 10 min and protein concentration measured with the DC assay (Bio-rad). For immunoblot analysis ...
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bioRxiv - Microbiology 2022Quote: ... and growth factor protein expression was quantified using the Bio-Plex Pro Rat Cytokine 23-plex assay (Bio-Rad Laboratories, Inc). Analytes included in the assay were ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred into a new tube and and the protein concentration was determined using a Bradford assay (Biorad #5000006) as described by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... 5’-ATG GGT TCG CAT GGT CCT AAT GAC ACA C-3’) (Cousineau et al., 2022) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 µL reaction contained 500 ng of total RNA ...
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bioRxiv - Physiology 2022Quote: ... Supernatants were transferred to a new tube and an aliquot were used for protein quantification using the Bradford assay (Bio-Rad). A total of 10-20 μg of protein lysates were separated on 8% gels by SDS-PAGE and transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2022Quote: ... The qPCR assay and data analysis were performed using a CFX96 Touch Real-Time PCR Detection System (Biorad, Hercules, CA, USA). The amplification and quantification conditions used were an initial denaturation step of 3 min at 95°C ...
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bioRxiv - Microbiology 2022Quote: ... with target specific primers and the assay ran on a CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). A 139-bp fragment was amplified for FBN11228 (F-CCAGCATCGGTACAACGGAA and R-AAGCTCGTGTTTTCGTGCTG) ...
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bioRxiv - Bioengineering 2022Quote: ... All the eluted fractions were tested using a Bradford reagent assay (diluted 1:5 with ddH2O) (190 μL Bradford, 10 μL sample) (Bio-rad). Positive fractions were desalted using 100 kDa amicons (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration of the EV was determined using the Quick Start™ Bradford protein assay according to manufacturer’s guidelines (BioRad, UK). In short ...
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bioRxiv - Neuroscience 2020Quote: ... 7.5 μl of SsoAdvanced Universal SYBR Green Supermix and 1 μl of the following DNA templates (Prime PCR Assay, Bio-Rad): mt-Co2 (mitochondrial ...
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bioRxiv - Microbiology 2021Quote: ... Images of whole plate phage assay were captured using a ChemiDoc XRS+ imaging system using the epi-light filter (BioRad, USA).
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bioRxiv - Plant Biology 2021Quote: ... The qPCR assay was performed by using UltraSYBR Mixture (KWBIO, #CW2602) on the CFX ConnectTM Real-Time PCR Detection System (Bio-Rad). ACTIN was used as a normalization control and the relative expression of each test gene was calculated by the 2-ΔΔCT method (Livak & Schmittgen ...
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bioRxiv - Biochemistry 2020Quote: ... steroid concentrations or their ratios were normalized to the total protein content of the incubations as determined in the supernatant after cell lysis using the DC Protein Assay (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was transferred to a new tube and protein concentration was determined using Protein Assay Dye Reagent Concentrate (Bio-Rad) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Fifty microliters of homogenate were used to measure cytokine and chemokine protein abundance using a Bio-Plex Pro mouse cytokine 23-plex assay (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: ... Concentration of the VLPs was estimated by Bradford assay and densitometry using SDS PAGE analysis of serially diluted VLP preparations on Mini-PROTEAN Precast Gels (Bio-Rad), stained with GelCode Blue Stain (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: Half of the produced supernatant (see above) was dosed by the Bradford method (Protein Assay Dye Reagent Concentrate, Biorad #500-0006) and digested with 1% trypsin (weight/weight ...
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bioRxiv - Microbiology 2020Quote: ... Samples were diluted 1:20 in 1X PBS and protein concentration was quantified by the DC protein assay with BSA as the standard (BioRad®). Lysates were normalized to 5 μg/μL in 2X loading buffer (Lysis buffer supplemented with 5 mM beta-mercaptoethanol and Coomassie blue) ...
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bioRxiv - Molecular Biology 2020Quote: ... Glycerol was added to the supernatants (final concentration: 10%) and protein concentrations were adjusted using Bio-Rad protein assay (Bio-Rad). The protein samples were diluted in 6x loading buffer (9.8% glycerol ...
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bioRxiv - Microbiology 2021Quote: ... using a TaqMan-based assay for lipL32 [180] in 25 μl reactions performed with SsoAdvanced Universal Probes Super Mix (Bio-Rad). Copy numbers for lipL32 were determined using an internal standard curve for the lipL32 amplicon cloned into pCR2.1 TOPO (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... Cytokines in the supernatants were then measured by using Bio-Plex Pro Mouse Cytokine 23-plex Assay (Biorad Laboratories, Hercules, CA) following the manufacturer’s standard protocol.
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bioRxiv - Microbiology 2021Quote: ... HIV-1 copy number was determined by a total HIV-1 DNA assay on droplet digital PCR (Bio-Rad, QX200 system), as described previously (Rutsaert et al. ...
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bioRxiv - Immunology 2020Quote: ... The concentrations of mouse and human IL-6 and TNF-α were determined by a multiplex Bio-Plex assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The amount of protein in the extracts was assessed by the Bradford method using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad). Protein electrophoresis in 10% or 12% PAA gels with SDS in 1x Laemmli buffer was carried out ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was used to quantify the total protein concentration in the sample using the DC protein assay (Bio-Rad) a BSA standard curve [34] ...
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bioRxiv - Bioengineering 2020Quote: ... The concentration of each fraction of proteins was determined by SDS-PAGE and quantitatively by Bradford protein assay (BIO-RAD, USA).
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bioRxiv - Cell Biology 2021Quote: ... Protein content was measured by Bradford assay and 30 mg of protein per sample were loaded into 4-20 % precast SDS-PAGE gels (Bio-Rad) under denaturing conditions ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Incorporation of radioactive amino acids was determined by liquid scintillation counting and normalized to the cellular protein content (quantified with DC Protein Assay (Bio-Rad)).
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bioRxiv - Immunology 2021Quote: ... the beads were re-suspended in assay buffer and the plates were read on a Bio-Plex 3D Suspension Array System (Bio-Rad). The following antigens were coupled with carboxylated beads (Luminex Corp) ...
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bioRxiv - Immunology 2022Quote: ... was centrifuged at 500 x g for 10 min Protein levels in the supernatant were measured by Bradford assay (Bio-Rad), and cytokine levels were measured using a custom multiplex ELISA (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in Native NiNTA lysis buffer (50mM NaH2PO4, 300mM NaCl, pH 8, 10mM imidazole) and protein concentration was measured via Bradford assay (Bio-Rad). 500-800μg of protein were incubated with NiNTA beads at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was estimated colorimetrically using a Bio-Rad protein assay and 25 μg of protein with equal volume of 2X Laemmli sample buffer (Bio-Rad) was denatured at 70°C for 10 min ...
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bioRxiv - Immunology 2021Quote: Lysates were centrifuged for 15 minutes at 4°C to recover supernatant and quantified for protein concentration using Protein Assay Dye Reagent Concentrate (Bio-Rad). 40ug of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... the primer pairs (Table 2; exception: S. umbla) were used to create EvaGreen-based droplet dPCR assays using the AutoDG (Bio-Rad) for droplet generation ...
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bioRxiv - Immunology 2021Quote: Protein analysis was performed using Bioplex assays (27-Plex, CXCL-1-, CXCL-2-, CXCL-5-, CCL22-single plex) (Bio-Rad Laboratories) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: Samples from m7GTP pull-down assays and lysates were boiled at 70°C for 10 min in Laemmli sample buffer (Bio-Rad) and resolved in 10% or 4-15% gradient SDS-PAGE gels ...
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bioRxiv - Immunology 2022Quote: ... the beads were re-suspended in assay buffer and the plates read on a Bio-Plex 3D Suspension Array System (Bio-Rad). Antigens and controls included BSA ...
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bioRxiv - Developmental Biology 2022Quote: ... The total content of glycogen was normalized to content of soluble protein measured using the Bradford assay (Bio-Rad 500–0006) with BSA standard curves.
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bioRxiv - Biophysics 2022Quote: ... Resulting membranes were resuspended in 100 μL solubilization buffer (1xPBS, 1% DDM, EDTA-free protease inhibitors) and total protein concentration was quantified using DC protein assay (Bio-Rad). For each condition ...
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bioRxiv - Cell Biology 2022Quote: ... After centrifugation at 14000 rpm for 20 min at 4°C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). Fifty micrograms of total extract were then loaded in SDS-polyacrylamide gels and transferred onto nitrocellulose membranes for western blot analysis ...
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bioRxiv - Cell Biology 2022Quote: ... as well as the level of cytokines in mouse serum, were assessed by using customized plates (Luminex assay, R&D) and analyzed on a Bio-Plex 200 instrument (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... and primers to the editing locus producing amplicons of <500bp (1× assay, 900-nM primers and 250-nM probe) using ddPCR supermix for probes (no deoxyuridine triphosphate [dUTP]) (Bio-Rad). Reference reactions were simultaneously performed using a 5’ HEX labeled probe/ primer combination targeting the houskeeping gene B2M ...
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bioRxiv - Neuroscience 2022Quote: ... culture supernatants were collected and cytokine levels in the samples were measured using a Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... conditioned culture medium was collected after 24 h and assessed by Luminex assay (Bio-techne) and a Bioplex-200 machine (Bio-Rad). Bioplex manager 6.1 software was used to determine analyte concentrations.
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bioRxiv - Neuroscience 2022Quote: The entire volume of each flow through obtained from the pull-down assay was separated on Mini-PROTEAN TGX gels (Bio-Rad) and proteins transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Immunology 2022Quote: ... Cytokines concentrations in BALF were assessed using a Bio-plex magnetic bead assay (Luminex, Biotechne, France) and analyzed using a Bio-plex 200 workstation (Biorad, France).
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bioRxiv - Physiology 2024Quote: ... Pierce™ Protease& Phosphatase Inhibitor Mini Tablets) and final protein concentration was determined with BCA protein Assay (Biorad Laboratories, Feldkirchen, Germany). Heart tissue samples of the infarct areas were excised (2 weeks or 8 weeks post-surgery ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 30 min at 20,000 x g at 4 °C and protein concentrations of the supernatants were measured with standard Bradford assay (Bio-Rad Laboratories). 25 or 50 µg of proteins were diluted in Laemmli buffer 3× (188 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... we used the multiplex bead array system Bio-Plex Pro Mouse Chemokine Assay Panel 31-Plex (#12009159, Bio-Rad Laboratories GmbH), according to the manufacturer’s instructions ...