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Citations for Bio-Rad :
8401 - 8450 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was then centrifuged through a 0.45-micron filter at 4,000 x g and the resulting cell extract was measured using the DC-Lowry assay (BioRad). One milligram of total cellular protein was then incubated with 30 μl bed volume of anti-V5 beads (Sigma) ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentrations were determined using the Bio-Rad Assay according to the manufacturer’s instructions (Bio-Rad Laboratories, Munich, Germany) based on the method of (48).
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bioRxiv - Pathology 2021Quote: ... The concentration of proteins was determined using a Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, #5000006). A total of 15 μg of protein from each sample were mixed with NuPAGE™ LDS Sample Buffer (Thermofisher ...
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bioRxiv - Cell Biology 2021Quote: ... The remaining mitochondrial pellet is suspended in MSHE+BSA and the concentration measured using a Bradford assay (Bio-rad).Isolated mitochondria are diluted 1:10 in ice cold mitochondrial assay solution (MAS ...
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bioRxiv - Cell Biology 2021Quote: ... 15 min) and the protein amount was quantified with the use of Bio-Rad Protein Assay (Bio-Rad, 5000006). For western blotting the following primary antibodies were used ...
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bioRxiv - Microbiology 2021Quote: ... All qRT-PCR assays were performed on the CFX96 Touch™ Real- Time PCR Detection System (BioRad, Hercules, USA).
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bioRxiv - Neuroscience 2021Quote: ... Radiolabel incorporation was measured with a scintillation counter and samples were subjected to a protein concentration assay (Bio-Rad). Data was analyzed as counts per minute per microgram of protein ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration of lysates was measured using the BIO-RAD DC protein assay according to the manufacturer’s instructions (Biorad). For precipitation experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was then transferred into a new tube and protein concentration was determined by BCA assay (Bio-Rad). Immunoblotting was performed using CDCP1(D1W9N ...
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bioRxiv - Genetics 2021Quote: ... Total protein level in the samples was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (#5000006, Bio-Rad).
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bioRxiv - Immunology 2020Quote: ... The total amount of proteins in lysates was quantified by the DC™ Protein Assay (500-0116JA; Bio-Rad) and 2104 EnVision Multilabel Plate Readers (PerkinElmer ...
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bioRxiv - Bioengineering 2020Quote: Protein concentration from each secretome was estimated using the Bio-Rad protein assay reagent (Bio-Rad, Hercules, CA, USA) and bovine serum albumin as standard ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were cleared of debris by centrifugation at 15,000 x g for 15 min at 4°C and then normalized for protein content using Bradford assay (BioRad). Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were sonicated on ice for 30 seconds and quantified using a DC™ Protein Assay (Bio-Rad, 5000113) before loading 10 μg of protein to 6-10% polyacrylamide gels ...
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bioRxiv - Cancer Biology 2022Quote: ... and protein levels were quantified using BCA Assay (Pierce) and supplemented with 4x Laemmli Buffer with 2-mercaptoethanol (Biorad). Samples were boiled and sonicated ...
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bioRxiv - Plant Biology 2022Quote: ... 4 °C) and the supernatant was taken up and the protein concentration was determined using the Bradford assay (Biorad).
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentrations of culture medium and cell lysate were determined by the Bradford assay (Bio-Rad, Hercules, CA). Culture medium or cell lysate samples were resuspended in SDS sample buffer containing β-mercaptoethanol and incubated at 95°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: All the assays were performed using the QX200 Droplet Digital PCR system (Bio-Rad Laboratories Inc., Pleasanton, CA, USA) according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... was used for qPCR assay in 20 μL of reaction mixture on a real-time PCR system (Bio-Rad) with the primer pairs listed in Table S2 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were passed through a 26G syringe and protein was quantified by DC Protein Assay (Bio-Rad, cat #5000112). Lysates were diluted to equal volumes and 30 ug protein was loaded per well using the Novex WedgeWell Tris-Glycine Welcome Pack (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were quantified by Pierce Reducing Agent Compatible BCA Protein Assay and run on 4-20% TGX gels (Biorad). Membranes were probed with anti-FLAG M2 antibody (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration of the samples was determined by the BioRad Protein Assay method (BioRad Laboratories, Hercules, CA, USA). For our gels ...
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bioRxiv - Biochemistry 2023Quote: ... The protein concentration of each lysate was analyzed using Bio-Rad protein assay reagent according to the manufacturer’s instructions (Biorad). Luciferase units obtained were normalized to the protein content of each well ...
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bioRxiv - Biophysics 2024Quote: ... Total protein concentration in purified microsomes and RyR1 fractions was measured with Quick Start Bradford Protein Assay (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... both proteins were supplemented with 5 mM MgCl2 and protein concentration was determined using a colorimetric assay (Bio-Rad). Proteins were then mixed at a 2.5:1 mass ratio of KtrC to N-terminal Strep-tagged KtrA (11 mg of KtrC and 4.5 mg of N-terminal Strep-tagged KtrA in 10 mL total volume of 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was normalized to 1 mg/mL using 0.1% Azo buffer by the Bradford assay (Bio-Rad). Samples were reduced with 30 mM DTT for 1 h and subsequently alkylated with 30 mM iodoacetamide for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... and screened for successful KI using mutation specific custom designed KASP genotyping assays (LGC) and KASP V4.0 2x Master mix (LGC) on a CFX384 Touch Real-Time PCR Detection System (BioRad). Alternatively ...
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bioRxiv - Cell Biology 2023Quote: ... 2mM DTT) until no protein was eluted as monitored by the Bio-Rad protein assay (Bio-Rad, Catalog #5000006). Proteins were eluted with 10 ml elution buffer (50mM Tris-HCl (PH=8),150mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... The burden in blood and tissues was determined by qPCR with the Sso Fast EvaGreen Supermix assay (Bio-Rad) using the flaB2 gene ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf lysates were cleared by centrifugation at 10,000×g for 5 min and total protein normalized via Bradford Assay (Biorad). Extracts were prepared for SDS-PAGE with a 5x loading buffer containing 10% (w/v ...
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bioRxiv - Biochemistry 2023Quote: Thermal shift assays were carried out in 96 well plates using the CFX96 RT-PCR detection system (Bio-Rad). Thermal stability experiments were performed in the reaction mixture containing LIG1 (5 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein concentrations of sample supernatants were measured with the Bio-Rad protein assay dye reagent (Bio-Rad, 5000006). Equivalent amounts of protein samples were mixed with Laemmli buffer (100 mM Tris-HCl pH 6.8 ...
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bioRxiv - Immunology 2023Quote: ... Lavage fluid and plasma cytokine quantities were measured by Bioplex Pro mouse cytokine 23-plex assay (Bio-Rad #M60009RDPD) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The total protein concentration was determined using Bradford protein assay according to the instructions from the manufacturer (Bio-Rad). POSTN protein expression from the ELISA assay was normalized with the total protein levels ...
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bioRxiv - Plant Biology 2023Quote: ... Ten µl of the supernatant were used to determine protein concentrations by the dye-binding Bradford assay (Bio-Rad). Twenty-five µg of total proteins in a final volume of 100 µl were pipetted into a white 96 well plate (Greiner Bio-One ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were centrifuged (15000 rpm at 4°C) and determined using protein assay dye reagent (Bio-Rad, 500006). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... clarified by centrifugation at 13.2K rpm and protein concentration was determined by BCA assay (Bio-Rad, Hercules, California, USA). For western blot analysis ...
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bioRxiv - Developmental Biology 2023Quote: ... and aliquots were 1:50 in water prior to Bradford protein assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006). Samples were then normalized to 1 mg/mL protein in 0.1% Azo prior to reduction with 30 mM DTT at 37C for 1 h and alkylation with 30 mM chloroacetamide for 45 min ...
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bioRxiv - Cell Biology 2023Quote: ... and dsacas9 transcripts were quantified using SYBR green PCR assays with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, 1725270). Mouse GAPDH mRNA was used as the house keeping control for quantification of Sacas9 ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR (RT-qPCR) assays were performed on a CFX96 real-time PCR system (Bio-Rad, Hercules, CA, USA) using SsoAdvanced Universal SYBR Green Supermix (12 μL final mix per reaction ...
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bioRxiv - Neuroscience 2023Quote: ... Protein content of supernatant was quantified via Bradford assay according to the manufacturer’s instructions (catalogue # 500-0006, Bio-Rad). Absorbance was measured at 595nm using a Pherastar FS (BMG LABTECH).
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bioRxiv - Neuroscience 2023Quote: ... were centrifuged at 20,000 x g for 25 min at 4⁰C and supernatants were assayed for total protein (Bradford assay, BioRad). Aliquots of WCL (30 µg total protein ...
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bioRxiv - Developmental Biology 2023Quote: ... The protein concentration das measured of each eluate sample in a 1:20 dilution by Bradford (BioRad protein assay). Per sample ...
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bioRxiv - Molecular Biology 2024Quote: ... Ribosome pellet was resuspended in same lysis buffer and protein concentration was determined via Bradford Assay (Bio-Rad, #5000205) prior to western blot.
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bioRxiv - Genomics 2024Quote: ... (Cat #10031243) was used in addition to a custom FAM Copy Number Assay targeting GFP (Bio-Rad, Cat #10042958). After assembling the ddPCR reactions ...
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bioRxiv - Cell Biology 2024Quote: ... 1 tablet EDTA-free protease inhibitors) and protein content was determined using a Bradford protein assay (Bio-Rad, #5000001). 50-100 µg protein was run on an SDS-PAGE and blotted using a wet-transfer system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative PCR (RT-qPCR) assays were performed on a CFX96 real-time PCR system (Bio-Rad, Hercules, CA, USA) using SsoAdvanced Universal SYBR Green Supermix (12 μL final mix per reaction ...
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bioRxiv - Neuroscience 2024Quote: The total amount of protein present in the samples was quantified using the Bio-Rad Protein assay (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration of the TX100-soluble fraction was measured with Dc Protein Assay (Biorad cat. no. 500-0116), added Laemmli buffer ...
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bioRxiv - Microbiology 2024Quote: ... and cytokines release was assessed using Bio-Plex Pro Human Cytokine Th1/Th2 Assay (Bio-Rad Laboratory, Hercules, USA) on a FLEXMAP 3D® analyzer (Luminex ...