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Citations for Bio-Rad :
8351 - 8400 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: Proteins were separated by sodium dodecyl sulphate (SDS)-polyacrylamide-gel electrophoresis (PAGE) and blotted onto polyvinylidene fluoride (PVDF) membranes (1704272; Bio-Rad, USA) using a Trans-Blot Turbo (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Equal amounts of cellular proteins were resolved on 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE) and subsequently transferred to nitrocellulose membranes (Bio-Rad Laboratories). Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 40 µg of protein from each sample was resolved on a 12% polyacrylamide gel via SDS-PAGE and subsequently transferred to a PVDF membrane (Bio-Rad, 1620177) using a wet transfer system ...
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bioRxiv - Physiology 2024Quote: ... Denatured and reduced protein was subjected to SDS-PAGE on 10-14% gels followed by transfer onto 0.2 μm low-fluorescence polyvinylidene difluoride membrane (Bio-Rad, Mississauga, Canada) and blocked with Li-COR Intercept Blocking Buffer for 1 hour (LI-COR ...
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bioRxiv - Cancer Biology 2024Quote: ... After SDS SDS-PAGE and transferring to a PVDF membrane using the Transblot® SD Semi-Dry Electrophoretic Transfer Cell (Product #1703940, Bio-Rad), PVDF membranes were blocked in 5% skim milk for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... The gels were rinsed in ddH2O before the proteins were transferred to 0.45 µM low auto-fluorescence PVDF membranes (Bio-Rad, UK, #1620261).
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bioRxiv - Biochemistry 2024Quote: We loaded 750 ng of Aβ42 peptide onto a 0.2 µm nitrocellulose membrane of the Bio-Dot Apparatus (Bio-Rad Cat. No. 1706545). Then the membrane washed twice with TBS containing 2% formaldehyde and then incubated with the same solution for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed four times with PBST for 5 minutes and exposed to ECL substrates (Clarity™ Western ECL Substrate, Bio-Rad and SuperSignal™ West Femto ...
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bioRxiv - Cell Biology 2024Quote: ... Gel was transferred at 350 Amperes for 120 minutes onto pre-activated polyvinylidene difluoride (PVDF) or Nitrocellulose membranes in a Mini-Protean® 3 Cell (BioRad Laboratories). Correct protein transfer was checked by red Ponceau S Solution (Panreac) ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples were denatured in Laemmli buffer at 96°C for 5 min and then equivalent amounts were loaded into SDS-PAGE gels for separation and transferred to nitrocellulose membranes (Cytiva, Marlborough, MA) using semi-dry transfer (BioRad, Hercules, CA) using standard techniques ...
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bioRxiv - Cell Biology 2024Quote: ... and then transferred onto a polyvinylidene fluoride (PVDF) membrane at constant voltage of 25V for 3 min using Trans-Blot TurboTransfer Packs (Cat.: 1704156, Lot.: 64340580, Bio-Rad, USA) and Trans-Blot Turbo system (Cat. ...
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bioRxiv - Microbiology 2022Quote: ... the cDNA was synthesized using the cDNA synthesis kit (iScript cDNA Synthesis Kit, Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Protein concentrations were determined using unlabeled wild-type proteins (β or PCNA) as standards in a Bradford assay (BioRad). Fluorophorophore concentrations were determined from absorbance measurements and molar absorptivities as described previously (9,10).
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bioRxiv - Cell Biology 2020Quote: All Rab34 used in steady-state assays was treated with AG 1-X8 anion exchange resin (Bio-Rad, 1401441) immediately before use to remove bound GDP ...
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bioRxiv - Immunology 2021Quote: ... and protein was quantified from the supernatant of each sample using the DC protein assay (Bio-Rad Laboratories, Inc.). NuPAGETM LDS sample buffer (ThermoFisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... Editing efficiency within each sib was assessed using digital droplet PCR (ddPCR) and Genome Edit Detection assays (Bio-Rad). Two sibs with highest editing efficiencies were selected ...
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bioRxiv - Microbiology 2022Quote: ... NS5B-FAM probe: 5’-ATGGGTTCGCATGGTCCTAATGACACAC-3’) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 μL reaction contained 500 ng of total RNA ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μl of lysate was used for protein determination by the DC Protein Assay (Bio-Rad Laboratories, Hercules, CA).
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bioRxiv - Systems Biology 2020Quote: Whole cell fractions were isolated using RIPA buffer and protein concentrations were determined using a Bradford Assay (Bio-Rad). 20µg whole cell lysate were separated on 8-12% SDS-PAGE gels (depending on the molecular weight of the target proteins) ...
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bioRxiv - Biochemistry 2021Quote: The thermal shift assay was performed on a CFX Connect™ Real-Time System (Bio-Rad, California, United States). FIH-1 wild-type and L340R were dialyzed against Na-phosphate buffer composed of 35 mM Na-phosphate (pH 7.5) ...
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bioRxiv - Genetics 2021Quote: ... protease inhibitor cocktail) and protein concentrations were determined by Bradford protein assay (Bio-Rad Laboratories, Inc. Hercules, CA, USA) and absorbance was measured at 595 nm on the Tecan Infinite® 2000 PRO plate reader (Tecan ...
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bioRxiv - Neuroscience 2020Quote: ... All assays included technical and biological triplicates were analyzed using the Sso Advanced Universal SYBR Green Supermix (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... or 20 ng of cDNA for mRNA assay was used per real-time PCR with SYBR Green (Bio-Rad) to detect DNA ...
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bioRxiv - Cell Biology 2021Quote: ... lysates were cleared by centrifugation at 20,000 x g for 30 min and protein amount was determined by standard Bradford assay (Biorad). For each reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were clarified by centrifugation and the protein concentration was determined using DC protein assay system (Bio-Rad Laboratories). Equal amount of protein was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... The concentration of the purified protein extracts was measured using the Bradford method (Protein Assay Dye Reagent, Bio-Rad). Purified His-tagged proteins were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris Protein Gels ...
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bioRxiv - Plant Biology 2020Quote: The total protein in each extract was quantified using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA), and 24 μg run in a 10% SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... The protein concentration of each homogenate was measured using a Bradford assay according to the manufacturer’s instructions (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein concentrations in the cell lysates (mg/mL) were assayed using the Bradford protein assay (Bio-Rad, Hercules, CA) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the lysates were cleared by centrifugation at 16,000 × g for 15 min and protein concentrations were estimated using QuickStart Bradford Protein assay (BioRad). After combining the same amount of proteins from each label ...
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bioRxiv - Genetics 2021Quote: ... Total protein level in the samples was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (#5000006, Bio-Rad).
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bioRxiv - Genetics 2021Quote: ... Total protein level in the samples was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (#5000006, Bio-Rad). One unit of Acetyl-CoA Carboxylase activity is defined as the enzyme generates 1 nmol of PO43-per hour.
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bioRxiv - Biophysics 2020Quote: ... the motif was dialyzed overnight in a Slide-A-Lyzer dialysis cassette (MWCO 10,000) and the concentration was measured using a Bradford assay (Biorad).
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bioRxiv - Systems Biology 2022Quote: ... The supernantant was then added to a new Eppendorf and protein concentration was assessed using the Bradford assay (Biorad).
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bioRxiv - Systems Biology 2022Quote: ... The post nuclear lysates were collected and protein concentration was measured with the Bio-Rad Protein Assay (Bio-Rad). An equal amount of protein was mixed with 4X NuPage LDS sample buffer (Thermofisher ...
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bioRxiv - Microbiology 2022Quote: ... The concentration of LL-37 and GCN4-M87/LL-37 complex was measured using the Bradford assay (Bio-Rad) with BSA as a standard.
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bioRxiv - Synthetic Biology 2022Quote: Protein concentrations of the cell lysates were determined using a Bio-Rad Protein Assay Dye Reagent (Bio-Rad, 5000006). Volumes of lysates containing equal amounts of total protein were boiled at 100°C for 5 minutes after addition of 1/4 volume of 4x SDS-PAGE Loading Solution (250 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... Total protein concentrations were determined using the Bradford method38 (Bio-Rad Protein Assay, Bio-Rad Lab, Mississauga, ON, Canada) and immunoblotting was performed as described previously39 ...
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bioRxiv - Microbiology 2022Quote: ... and screened for successful KI using mutation specific custom designed KASP genotyping assays (LGC) and KASP V4.0 2x Master mix (LGC) on a CFX384 Touch Real-Time PCR Detection System (BioRad). Homozygous KI was confirmed by Sanger sequencing after amplification using primer SAM_Seq_Gen58570_FW (5’-CAT GAA GGC TCT TCC TGC GTA A ...
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bioRxiv - Biochemistry 2022Quote: ... Bio-Rad PrimePCR Assay gene-specific primers were used to test RSL24D1 mRNA levels (Bio-Rad 10025636; RSL24D1, qHsaCID0021318) and the following primers were used for the 7SL RNA forward ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein concentration for both cytosolic fraction and mitochondrial fraction was then determined by DC Protein Assay (Bio-Rad), and 175 μL of 100–500 μg/ml protein solution was generated with assay buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were harvested and cytokine secretion assessed using BioPlex assay (Luminex MAGPIX Instrument, Bio-Rad, Marne-la-Coquette, France) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: The concentrations of protein in the supernatant were determined by using the Bio-Rad protein assay reagent (Bio-Rad). Samples ...
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bioRxiv - Microbiology 2021Quote: ... Reaction mixtures (20 μl) for all assays contained 1X SsoAdvanced Universal SYBR Green Supermix (Bio-Rad Inc., Hercules, CA), 600 nM (each ...
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bioRxiv - Microbiology 2021Quote: ... the remaining interphase was dried completely before resuspending in Tris-buffered saline (50mM Tris-Cl, 150mM NaCl, pH 7.6) for protein quantification using a Bradford Assay (BioRad). Aqueous phase was evaporated under nitrogen flow and used for LC-MS ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine concentrations in serum were measured using a mouse 23-cytokine Bio-Plex Pro Assay according to the manufacturer’s instructions (BioRad). Concentrations for human cytokines were determined using the ProcartaPlex Multiplex Immunoassay (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... coli HU protein was monitored using 20% SDS-PAGE and the concentration was determined using the Lorry assay (Biorad). The purified E ...
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bioRxiv - Synthetic Biology 2022Quote: Temperatures used in the assays were implemented by a user-defined gradient protocol on a thermocycler (Bio-Rad, C1000). TIED-lipases (spore OD600 = 0.5 ...
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bioRxiv - Immunology 2022Quote: ... Protein concentration in cell lysates was determined by using a standard Coomassie Bradford protein assay (Bio-Rad, Hercules, CA). For SDS-PAGE cell lysates were mixed with an equal volume of 2X SDS sample buffer (50 mMTris ...
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bioRxiv - Plant Biology 2020Quote: ... This activity was then standardized to the quantity of total proteins present in the sample (Bradford assay, Bio-Rad). For inhibitor assays ...