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Citations for Bio-Rad :
8451 - 8500 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Total protein concentration was estimated using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate, Bio-Rad Laboratories, #5000006).
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bioRxiv - Biochemistry 2024Quote: ... Protein content of whole cell lysates was determined using the DC protein Assay (Bio-Rad, Cat # 5000113 and 5000114) with absorbance at 750 nm measured on a CLARIOstar plate reader ...
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bioRxiv - Molecular Biology 2024Quote: ... Ribosome pellet was resuspended in same lysis buffer and protein concentration was determined via Bradford Assay (Bio-Rad, #5000205) prior to western blot.
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration of the TX100-soluble fraction was measured with Dc Protein Assay (Biorad cat. no. 500-0116), added Laemmli buffer ...
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bioRxiv - Physiology 2024Quote: ... The total protein concentration of the homogenates was measured with Bio-Rad Protein Assay Dye (#5000006, Bio-Rad Laboratories). The samples were stored frozen at −80°C overnight followed by digestion ...
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bioRxiv - Molecular Biology 2024Quote: Fluorescence-based detection assays were performed using a CFX96 touch real-time PCR system (Bio-Rad, Hercules, CA, USA). The assays began with the preparation of a reaction mixture consisting of 250 nM Cas12a ...
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bioRxiv - Microbiology 2024Quote: ... and cytokines release was assessed using Bio-Plex Pro Human Cytokine Th1/Th2 Assay (Bio-Rad Laboratory, Hercules, USA) on a FLEXMAP 3D® analyzer (Luminex ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations in yeast lysates were determined using the Bio-Rad Protein Assay reagent (Bio-Rad GmbH, Munich, Germany) against a BSA standard curve ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were collected and proteins were quantified using Quick StartTM Bradford Protein Assay (Bio-Rad Laboratories, USA, Cat # 5000201). Equal amounts of protein extracts (30 µg ...
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bioRxiv - Cell Biology 2024Quote: ... RT-qPCR was carried out in technical triplicate using Taqman assays and SsoAdvanced Universal Probes Supermix (Bio-Rad; 1725284), following manufacturer protocols and using 1 uL diluted cDNA input per 10 uL reaction ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted proteins were separated by NuPAGE 4-12% Bis-Tris gel in MES SDS running buffer and transferred to a PVDF membrane (Bio-Rad, 162-0263). Tubulin beta chain protein was detected with an anti-TUBB antibody (1:500 in 5% fat-free milk solution in TBST ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 µg DNA from each sample was transferred to a nitrocellulose membrane using the Bio-Dot SF microfiltration apparatus (Bio-Rad, #170-6542). The membrane was blocked in 5% non-fat milk in TBS with 0.1% Tween-20 ...
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bioRxiv - Cell Biology 2020Quote: ... 20 mg of total protein was separated by SDS-PAGE on polyacrylamide gels (10%) and transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories, Inc.). The membrane was probed with antibodies against Flag (#F7425 ...
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bioRxiv - Cell Biology 2020Quote: ... Twenty micrograms of protein were loaded on 4-15% gradient TGX Stain-Free™ Protein Gels and transferred to Immun-Blot® LF PVDF membrane (Bio-Rad). The membranes were blocked with 1X TBS with 0.05% Tween 20 and 5% skim milk powder for 2 hours at 25°C ...
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bioRxiv - Pathology 2021Quote: ... Hippocampal samples with equal amounts of protein (40 μg) were separated by electrophoresis in 10% sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE) and transferred onto PVDF membrane (Bio-Rad Labs Inc.). Nonspecific binding sites on the membrane were blocked by incubation with 5% milk in Tris-buffered saline solution containing 0.1% Tween 20 at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated with SDS-PAGE and then later transferred to PVDF membranes by electrophoresis using a Bio-Rad Trans-Blot device (Bio-Rad, Richmond, CA). Blots were blocked for 1 h at room temperature or overnight at 4 °C with 5 % skim milk in Tris-buffer saline with Tween-20 (TBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... protein samples were resolved via SDS-PAGE and then transferred onto nitrocellulose membranes by the Trans-Blot® Turbo™ Transfer System (Bio-Rad). Membranes were blocked in 5% non-fat dry milk and hybridized with antibodies in TBST buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μg proteins were loaded and separated by SDS-PAGE gels (10%) electrophoretically and transferred to polyvinylidene difluoride membrane (Bio-Rad Incorporation, 10026933). Blocking was performed by adding 5% BSA for 60 min at room temperature on the shaker ...
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bioRxiv - Molecular Biology 2021Quote: ... The boiled proteins were separated on 4% to 15% SDS-PAGE gradient gels and transferred to LF PVDF membrane (Bio-Rad, Cat #1620260). The membranes were blocked in 5% skim milk in TBST (20 mM-Tris ...
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bioRxiv - Immunology 2021Quote: ... Samples from the pellets were resuspended in the loading buffer and were subjected to gel electrophoresis and then was blotted onto PVDF membranes (Bio-Rad, Hercules, CA). Membranes were blocked and incubated with specific primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 2 hours at room temperature with HRP-conjugated rabbit secondary antibody (Bio-Rad, USA, 170-6515; RRID: AB_11125142) for NALCN detection and HRP-conjugated mouse secondary antibody (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were transferred from the gel onto a 0.2 µm nitrocellulose membrane using the wet transfer method (Bio-Rad Laboratories, Hercules, CA). Overnight transfer was performed for 16 h using a constant voltage of 30V ...
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bioRxiv - Biochemistry 2020Quote: Cell or tissue protein lysates were separated by SDS-PAGE on pre-cast 8-16% polyacrylamide gels and transferred onto nitrocellulose membranes using a Trans-Blot® Semi-Dry transfer cell (all purchased from Bio-Rad Laboratories), blocked in non-fat milk and incubated overnight at 4°C with primary antibody for HILPDA (1:750 ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were separated by SDS-PAGE and blotted onto a PVDF membrane as recommended by the manufacturer (Transblot, Bio-Rad Laboratories, USA). The membrane was blocked overnight at 4 °C in a solution of 5 % (w/v ...
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bioRxiv - Plant Biology 2020Quote: Proteins were transferred from an SDS-polyacrylamide gel to a polyvinylidene difluoride (PVDF) membrane by electroblotting using a Trans-Blot SD transfer cell (Bio-Rad, www.bio-rad.com) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Protein samples were separated on 12% gels and then transferred to nitrocellulose membranes (Hybond-C; Amersham Life Sciences, UK) using a semi-dry transfer apparatus (Bio-Rad Laboratories, USA). The membranes were blocked with 5% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... transferred to 0.2 μm pore-size PVDF membranes, and blocked for 1 hour in blocking buffer (5% milk, 0.1% Tween-20 in 1x TBS (Bio-Rad, Hercules, CA, USA)) ...
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bioRxiv - Cell Biology 2021Quote: ... Similar amount of total cell lysate were fractionated through a 10% SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad Lab, California, USA). The proteins transferred to the membrane were visualized by Ponceau solution stain 0.3% for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were run on 5-20% SDS- polyacrylamide gels and transferred or spotted onto nitrocellulose membrane with 2µg/µl concentration using Bio-dot® Microfiltration Apparatus (Bio-Rad, Feldkirchen, Germany). Total protein amounts per lane were stained with No-stain™ Protein Labelling Reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were then transferred to a polyvinylidene difluoride (PVDF) membrane applying 20 mA for 20 minutes in a semi-dry transfer cell (Bio-Rad Laboratories, USA). The membrane was blocked in 0.5% powdered milk in TBS-T buffer at 4°C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... and an equivalent amount of protein lysate (12-15 µg) was electrophoresed on a 4-12% NuPAGE gradient gel and transferred onto low florescent polyvinylidene difluoride membranes (Bio-Rad, Cat: 1704274). Immunoblotting was performed with primary antibodies followed by secondary antibodies with the indicated dilutions ...
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bioRxiv - Microbiology 2022Quote: The protein samples denatured in 1x SDS sample buffer were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) in a 12% gel before blotted to polyvinylidene difluoride (PVDF) membrane by the wet transblot system (Bio-Rad, California, USA). The blocked membrane was incubated with the primary antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... SDS-PAGE and electrically transferred to a PVDF membrane (Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Packs, Bio-Rad, Hercules, CA, USA) using the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... Twenty μg of proteins were separated on 10% SDS-polyacrylamide gels and transferred onto polyvinylidene difluoride (PVDF) membranes in a Trans-Blot apparatus (Trans-Blot Turbo™, Bio-Rad, USA). Membranes were then saturated with a buffer containing 5% milk ...
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bioRxiv - Biochemistry 2021Quote: ... PVDF membranes were briefly immersed in methanol and then equilibrated in western transfer buffer (20% of Bio-Rad 5x transfer buffer, 20% ethanol) for 3-5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to nitrocellulose membrane by semi-dry transfer using the Trans-Blot Turbo Transfer System (BioRad, 1.5 A for 7 min). Membranes were blocked in 5% milk in 1x PBS with 0.1% Tween 20 (PBST ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated on 12% SDS-PAGE gels and transferred to polyvinylidene difluoride membranes (Cytiva Amersham™ Hybond™, USA) with Trans-Blot Turbo Transfer System (BioRad, USA). The membranes were blocked in 5% fat-free milk in phosphate-buffered saline containing 0.2% of Tween-20 (PBST ...
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bioRxiv - Bioengineering 2021Quote: Protein (50 μg) was separated by SDS□ polyacrylamide gel electrophoresis and transferred onto PVDF (polyvinylidene fluoride) membrane (Bio-Rad Lab Inc., Hercules, CA) by electro blotting ...
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bioRxiv - Physiology 2022Quote: ... ran on a 4-20% Criterion TGX Stain-Free Precast Gel and transferred to 0.2 µm PVDF membrane (Bio-Rad Laboratories, Inc, UK). Primary antibodies against FLAG (1:10,000 ...
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bioRxiv - Immunology 2022Quote: ... Protein samples were resolved by SDS polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA), followed by blocking for 1 h with 5% nonfat milk in Tris-buffered saline-Tween 20 buffer and probing with antibodies against Strep Tag (SAB2702215 ...
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bioRxiv - Cell Biology 2022Quote: ... and 60 μg purified 6xHis-CNAα copurified with regulatory subunit CNB were resolved by SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad, 162-0112). The membrane was stained with Ponceau S solution (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein (5–20 μg) was resolved onto 8–12% SDS-PAGE and blotted onto 0.2 μm pore size PVDF membranes (1620177, Bio-Rad, Hercules, CA, USA). Primary antibodies were incubated overnight at 4°C after blocking membranes for 1h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PPIases and PPIase fragments were vacuum aspirated onto a nitrocellulose membrane as serial dilution starting with 1 µg/ml using the dot blot system (Bio-Rad, Feldkirchen, Germany). Recombinant C3 toxin from C ...
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bioRxiv - Plant Biology 2020Quote: ... were centrifuged at 5000 x g for 15 min at 4°C and resolved by SDS-PAGE prior to transfer to a PVDF membrane (BioRad, Hercules, CA, USA). The membrane was then incubated with SnTox3 primary antibodies ...
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bioRxiv - Pathology 2020Quote: ... LC3-II and p-mTOR were separated by electrophoresis and then transferred onto the nitrocellulose membranes (Bio-Rad; Trans-Blot Turbo Transfer System). After several washes with TBST buffer ...
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bioRxiv - Microbiology 2020Quote: Equal amounts of protein from cell lysates were separated by SDS PAGE and transferred onto a nitrocellulose membrane by a semi-dry transfer method (Trans Blot® SD Semi-Dry Transfer cell, Bio-Rad, USA). Membranes were blocked with 5 % milk solution before incubation with primary antibodies at 1:1000 dilution unless otherwise stated ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were washed 3 x 10min in TBS + Tween 20 before proteins were detected by Immuno-Star luminol-peroxide (Bio-Rad, #170-5070). Bio-Rad Chemi-doc was used to visualize and quantify proteins with densitometry.
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bioRxiv - Biochemistry 2021Quote: ... Total proteins in the gel or on the membrane were visualized using Coomassie (InstantBlue, Sigma-Aldrich, scanned with the Odyssey or BioRad Chemidoc imagers) or Ponceau S staining ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated briefly at 90°C and then transferred to a Trans-Blot Turbo mini nitrocellulose membrane using Trans-Blot Turbo Transfer system (Bio-Rad, Hercules, CA). The membrane was incubated overnight in antibody buffer (50mM tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... total and surface labeled proteins were separated using a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (Bio-Rad, Feldkirchen, Germany). The membrane was blocked for 1 h in PBS-T supplemented with 5% skim milk and afterward incubated with 1:600 primary c-Myc-tag Polyclonal antibody (sc-789 ...