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Citations for Bio-Rad :
8701 - 8750 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... 4-degree Celsius) and the protein concentration in the supernatants was quantified using the DC protein assay (BIO-RAD, 5000116). Concentration was normalized before Western blot analysis ...
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bioRxiv - Biochemistry 2024Quote: ... The concentrate was quantified with a Bradford assay and the purity was assessed by SDS-PAGE using Image Lab v6.1 Software (Bio-Rad Laboratories Inc. ...
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bioRxiv - Bioengineering 2024Quote: Protein concentration levels of lysates were analyzed using a BCA protein assay and quantified using a colorimetric 96-well microplate reader (Biorad). A total of 4 μg of protein were extracted from each lysate and reduced with 1:1 dilution of 2-mercaptoethanol (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... protein concentration of cells lysed with mRIPA buffer containing protease inhibitor was measured using DC™ Protein Assay (Bio-Rad). Protein (20μg ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein quantifications were determined with a Bradford assay then 30μg of protein was separated on a 8-16% or 12% pre-cast gel (Bio-Rad) using primary antibodies diluted 1:500-1:1000 and secondary antibodies (1:2500 ...
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bioRxiv - Immunology 2023Quote: ... The second kit used was the Bio-Plex Pro Serology Reagent Kit (Bio-Rad, Cat No. 12014777), with human IgA positive and negative controls (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were run for ≈ 90 minutes at 150 V and transferred into nitrocellulose or PVDF membranes using the Trans-Blot® Turbo(tm) Transfer System (Bio-Rad, Deeside, UK) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 µg of WCE were separated on mini protein TGX stain-free gel and electroblotted using the PVDF transfer membrane (Bio-Rad, Hercules, CA, USA). The ChemiDoc™ MP imaging system (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... the cell lysates were separated by 15% SDS-polyacrylamide gel electrophoresis and electro-blotted onto a Immun-Blot PVDF Membrane using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 2% skimmed milk and probed with antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... resolved on the 12% SDS PAGE and transferred to nitrocellulose membrane (Trans-Blot Turbo Midi Nitrocellulose Transfer Pack, Bio-Rad, 0.2 μm pore size) using Trans-Blot Turbo Transfer Starter System (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates from each sample were separated by SDS-PAGE and transferred to an Immuno-Blot PVDF Membrane (162-0177, Bio-Rad Lab., CA, USA), then blocked with 1% skim milk in TBST (tris-buffered saline with 0.1% Tween-20 ...
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bioRxiv - Microbiology 2022Quote: ... was used to detect the heme signal on the transblotted PVDF membrane and imaging was performed with ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA). All the heme stain blots were washed with 50 mL stripping buffer (60 mM Tris pH= 7 containing 2% SDS and 7 µL/mL β-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... separated by 8% SDS-PAGE gels at 100V for 1 hour and then transferred to a 0.45 μm pore size nitrocellulose membrane (Bio-Rad, catalog number: 162-0115) at 0.2 A for 1.5 hours in an ice bath ...
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bioRxiv - Cancer Biology 2022Quote: Equal amounts of cell lysate were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Blocking was performed for 60 min with 5% nonfat dry milk in Tris-buffered saline and Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... For western blot analysis samples were run on 12% SDS-PAGE under reducing conditions and transferred to PVDF membrane (Bio-Rad, Cat. No. 1620177). SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Neuroscience 2021Quote: ... These samples were run on a 4–20% SDS-PAGE and electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad Laboratories, Hercules, CA, USA) from the gel by a semidry blotting apparatus (Bio-Rad Laboratories) ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on 10%–12% SDS polyacrylamide gels by electrophoresis and transferred onto nitrocellulose membranes using a Trans-Blot SD Semi-Dry transfer cell (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% skim milk for 1 h ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: Proteins were transferred from gels to PVDF membranes using a Trans-Blot® Turbo™ Transfer System and associated accessories (Bio-Rad Laboratories, Inc.). Membranes were probed with anti-Strep-II tag and anti-CB2 antibodies according to manufacturer instructions.
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bioRxiv - Biochemistry 2020Quote: ... resolved on the 8% SDS PAGE and transferred to nitrocellulose membrane (Trans-Blot Turbo Midi Nitrocellulose Transfer Pack, Bio-Rad, 0.2 µm pore size) with the use of a Trans-Blot Turbo Transfer Starter System (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of cell lysate or concentrated conditioned medium were resolved by sodium dodecyl sulfate– polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). The membrane was blocked for 60min with 5% non-fat dry milk in Tris-buffered saline and Tween 20 (1:1000) ...
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bioRxiv - Immunology 2020Quote: ... of protein were incubated in 1X Laemmli buffer with 0.1 M dithiothreitol at 65°C for 15 min and electrophoresed on 4-15% gradient TGX gels and transferred to PVDF membranes (Bio-Rad Laboratories, Mississauga, Canada). The membranes were blocked with Tris-buffered saline with 0.05% Tween 20 (TBST) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were transferred from polyacrylamide gel to polyvinylidine difluoride (PVDF) membranes as described by (Towbin et al., 1979) using a Trans-blot® electrophoretic transfer cell (Bio-Rad, city, country). The PVDF membrane with transferred proteins was blocked overnight at 4°C in blocking solution (5% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nuclear and total protein samples were separated on sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE) electrophoresis and subsequently transferred to polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA). After blocking using 5% of bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride membrane by using a semi-dry transfer unit (both from Bio-Rad, Hercules, CA, USA). After being blocked in Tris-buffered saline containing 5% non-fat milk and 0.1% Tween-20 ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were transferred on a PVDF membrane according to the manufacturer’s protocol (Trans-Blot® Turbo™ Rapid Transfer System, BIORAD, Les-Ulis, France) using a small protein transfer program ...
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bioRxiv - Immunology 2021Quote: ... supernatants or virions were separated on 10% SDS polyacrylamide gel under reducing conditions and transferred to a nitrocellulose membrane with a Trans-Blot Turbo System (Bio-Rad, Hercules, CA, USA). Filters were saturated for 1 h with 5% nonfat dry milk in TBST (TBS with 0.1% Tween 20 ...
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bioRxiv - Cell Biology 2022Quote: ... 30 μl were loaded and resolved on 6% sodium dodecyl sulfate (SDS) gels and transferred onto nitrocellulose membranes with Mini-PROTEAN III and Mini Trans-Blot cells (Bio-Rad, Hercules, CA, USA), respectively ...
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bioRxiv - Genetics 2022Quote: ... proteins were transferred to a pre-activated PVDF membrane using the Trans-Blot®Turbo™ Transfer System and visualized using ECL (Bio-Rad Chemidoc). Antibodies used in this study are ...
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bioRxiv - Neuroscience 2022Quote: ... Solid phase specificity was confirmed with dot blots of serial antigen dilutions (2 μL) applied to nitrocellulose membrane (Bio-Rad 0.45 µm; Fig. 1b). Membranes were allowed to air dry (1 h ...
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bioRxiv - Neuroscience 2023Quote: ... A 5 μL aliquot of the aSyn protein not incubated or incubated for different periods under aggregating conditions was transferred to a nitrocellulose membrane (Bio-rad®; 0.45 μm). The sample was then blocked with OdysseyTM blocking buffer for 60 min at 22° C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An equal amount of total protein was resolved by a 10% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Membranes were blocked and incubated with either anti Phospho-(Ser/Thr ...
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bioRxiv - Molecular Biology 2022Quote: 20µg of protein extracts or 10% Co-IP samples were resolved on 10-12% SDS–PAGE and proteins were transferred to PVDF membranes using a semi dry Western blot system (Bio-Rad Trans-Blot SD). Membranes were blocked in 5% non-fat milk in PBS-T (1x PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein were resolved by sodium dodecyl sulfate-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories, Hercules, CA, USA). Blots were probed with the indicated primary antibodies listed in Extended Data Table 4 ...
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bioRxiv - Neuroscience 2022Quote: ... PVDF membranes were briefly immersed in methanol and then equilibrated in western transfer buffer (20% ethanol, 20% of Bio-Rad 5x transfer buffer, #10026938) for 3-5 minutes ...
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bioRxiv - Genomics 2022Quote: ... XCell II Blot module was used for wet electrophoretic transfer of proteins from SDS-PAGE gel to nitrocellulose membrane (Biorad, Cat # 1620145, 0.45 μm) in Tris-Glycine Electroblotting buffer (National Diagnostic ...
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bioRxiv - Pathology 2024Quote: ... The SDS–PAGE of all dialyzer membrane extracts was performed according to the method described by Laemmli[19] using a Bio-Rad system (Bio-Rad Laboratories, CA, USA). 20 μg samples were separated by SDS-PAGE on 14% acrylamide gel and then stained with Coomassie blue ...
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bioRxiv - Molecular Biology 2024Quote: ... here the proteins were separated on SDS-gels and transferred onto a nitrocellulose membrane by the semi-dry Transblot Turbo Transfer System (Bio-Rad, Hercules, CA, USA). Primary antibodies were obtained from Agrisera (Vännäs ...
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bioRxiv - Cell Biology 2023Quote: ... Separated proteins were transferred from the gel onto the membrane by semi-dry blotting using the Trans-Blot Turbo™ Transfer System (Bio-Rad, Munich, Germany) with a discontinuous buffer system consisting of one cathode buffer and two anode buffers ...
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bioRxiv - Molecular Biology 2024Quote: ... Gels were run at 160 V for 1 hour before being transferred to 0.2 µM nitrocellulose membranes (Bio-Rad Trans-Blot Turbo Mini, 1704158) via a BioRad™ Trans-Blot® Turbo© Transfer system (7-minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... and transferred to PVDF transfer membrane (IPVH00010 Immobilon®-P; MilliporeSigmaTM, Burlington, USA) overnight at 10 mA in a cold room (Mini trans-blot cell; Bio-Rad, Hercules, USA). The membrane was blocked for non-specific sites and incubated with anti-HvHsfA6a in 1:2000 dilutions overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were transferred to a pre-activated PVDF membrane using the Trans-Blot®Turbo™ Transfer System and visualized using ECL (Bio-Rad Chemidoc). The following are the antibodies used in this study ...
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bioRxiv - Immunology 2024Quote: ... Cell lysates were separated on an 8-12% SDS-polyacrylamide gel and blotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA), The membrane was incubated overnight at 4°C with primary Abs ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of proteins (20 μg) were separated by SDS-PAGE and then transferred onto nitrocellulose membranes (ref 1704271, Bio-Rad, Hercules, California, USA). Membranes were incubated in blocking buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The resolved proteins were transferred on to a 0.22 µ nitrocellulose membrane (#10600004, Cytiva, US) for 60 min at 10 V using the Trans-blot semi-dry transfer apparatus (Bio-Rad Laboratories Inc., US). The blots were blocked with 4 % skimmed milk (#2325 ...
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bioRxiv - Microbiology 2024Quote: ... Equal protein amounts of the different samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose or polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories, Feldkirchen, Germany) by semidry blotting ...
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bioRxiv - Microbiology 2024Quote: ... The free dsDNA and shifted protein-DNA complexes in the gels were then transferred to a nylon membrane and cross-linked using GS Gene Linker UV Chamber (Bio-Rad Laboratories, Hercules, CA). The nylon membrane was incubated with 20 μg/mL Proteinase K (Ambion ...
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bioRxiv - Immunology 2024Quote: ... The samples on the gel were then transferred to a methanol-activated PVDF membrane using a semi-dry transfer system (BioRad Trans-Blot Turbo Transfer). The membrane was blocked with 5% non-fat dry milk or 5% BSA in 1 x Tris-Buffered Saline with 0.1% Tween-20 (TBS-T ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were separated using 10% SDS page and blotted on a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories, Inc., Austria). The membrane was probed with the anti pY-4G10 (mouse ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed 3x in PBST (10 min) and incubated for 1 hr with horseradish peroxidase conjugated anti-rabbit (BioRad 170-6515, lot #: 64033820) secondary antibodies (1:10000 ...
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bioRxiv - Molecular Biology 2023Quote: ... After gel migration proteins were transferred to Amersham nitrocellulose Protran Western blotting membranes (GE10600001) using semi-dry transfer (SEMI DRY BLOT APPARATUS, Bio-Rad, cat. no.1703940). Blots were blocked in TBS-T and 5 % milk and probed with relevant antibodies overnight ...