Labshake search
Citations for Bio-Rad :
8301 - 8350 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein samples (15 μg/lane) were subjected to analysis by 10% SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620264) using either semi-dry or wet electroblotting (for proteins with a molecular weight of 100 kDa and above) ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 5% BSA for 60min at RT and incubated with the primary antibody (anti-NP, Bio-Rad, 1/5000) for 45-60min shaking ...
-
bioRxiv - Molecular Biology 2023Quote: ... the samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were transferred to a polyvinylidene fluoride (PVDF) membrane (Bio-Rad, USA) using a semidry electrophoretic apparatus ...
-
bioRxiv - Plant Biology 2023Quote: ... Proteins were then blotted onto a PVDF membrane (pore size 0.2 µm) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad) and the “Mixed MW” protocol (7 min blotting time) ...
-
bioRxiv - Cell Biology 2022Quote: ... 1.5 µg of gDNA were dissolved in 250 µL TE buffer and loaded onto the membrane with the help of a Bio-Dot apparatus (Bio-Rad, 1706545) and a vacuum-pump ...
-
bioRxiv - Cancer Biology 2022Quote: ... prestained protein ladder were resolved on 10% acrylamide gels by SDS-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose membrane (BioRad Cat. # 1620112) at 100 volts for 90 min.
-
bioRxiv - Cancer Biology 2022Quote: ... His6MBP-F47R 4C/4S E6 and His6MBP-4C/4S E6 were spotted on a 0.45 µm nitrocellulose membrane (BioRad, Cat. # 162-0116) and allowed to dry at room temperature for 1 hour ...
-
bioRxiv - Bioengineering 2022Quote: ... while the other gel was transferred to a nitrocellulose membrane using the Trans-Blot Turbo Transfer system following manufacturer guidelines (Bio-Rad Laboratories). The nitrocellulose membrane was removed and placed in 1X Casein blocker for one hour on rotator followed by incubation with a 1:1000 dilution of his HRP-conjugated antibodies (Proteintech ...
-
bioRxiv - Cell Biology 2022Quote: Protein extracts were separated under denaturing conditions by SDS–PAGE and transferred to nitrocellulose membranes using a Trans-Blot Turbo nitrocellulose transfer pack (Bio-Rad, 1704159). Membranes were blocked for 45 min at room temperature using 1x StartingBlock buffer (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were blotted onto 0.45 µm nitrocellulose membrane (Amersham) overnight at a constant current of 200 mA using a Trans-Blot® Cell (Bio-Rad, USA).
-
bioRxiv - Microbiology 2022Quote: ... Proteins were run on a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the transfer to a nitrocellulose membrane (Amersham™ Protran®) was performed in a wet system (Transfer chamber mini-Protean, BioRad). Membranes were blocked with 5% low fat powdered milk (Roth ...
-
bioRxiv - Microbiology 2022Quote: ... the proteins were separated on a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, USA). The blots were blocked in 5% BSA in Tris-buffered saline with Tween 20 (TBST ...
-
bioRxiv - Molecular Biology 2022Quote: ... Either 50 μg or 25 μg (puromycin blots only) of total protein was separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620177) for blotting ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... the whole cell membrane pellets were resuspended in buffer and protein concentration was estimated by dye binding (Coomassie Brilliant Blue R-250; Bio-Rad, USA). The binding reaction was carried out in a final volume of 0.1 ml of Na+-containing buffer (20 mM Tris-HCl ...
-
bioRxiv - Plant Biology 2023Quote: Equal amounts (10 µg) of nuclear proteins from different samples were separated using SDS– PAGE and electrotransferred to a nitrocellulose membrane (BioRad, 162-0115) for 1.5 h at constant 400 mA at 4 °C ...
-
bioRxiv - Bioengineering 2022Quote: ... Fifteen micrograms total protein extracts were separated by 4–20 % stain-Free SDS-PAGE gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories). Membranes were blocked using Tris-buffered saline (TBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed as previously mentioned and the antigen-antibody complex was revealed by Clarity Enhanced Chemiluminescence Blotting Substrate (Bio-Rad, #1705060) using the iBright™ FL100 Imaging System (ThermoFisher) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 1 μg/μL of total cellular protein from each sample was used to run a PAGE and transferred onto a PVDF membrane (Bio-Rad, 1704157). The membranes were soaked in a blocking solution of 5% BSA (AMRESCO ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... purified protein fractions were separated with 10%–20% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry blotter (ATTO ...
-
bioRxiv - Systems Biology 2023Quote: ... was applied for 3 min before the membranes were imaged using the ChemiDocXRS+imaging system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Quantification of proteins was conducted using Image J software (Version 1.52a ...
-
bioRxiv - Bioengineering 2023Quote: ... All samples with equal amounts of protein were loaded on gels and separated by reducing SDS-PAGE and transferred to PVDF membranes (Bio-Rad, US). Membranes were blocked with 5% milk powder and incubated with rabbit anti-human Sox2 (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... The horse-radish peroxidase conjugated to the secondary antibody was detected by incubating the membrane with 2 mL chemiluminescence substrate (Bio-Rad, 1705062) for five minutes and then imaged using a ChemiDoc XRS+ imager (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2023Quote: ... After the membranes were washed 3x during 5 minutes in TBST and the chemiluminescence was detected using Clarity™ Western ECL Substrate (Bio-Rad) and ChemiDoc MP Imaging System (Bio-Rad) ...
-
bioRxiv - Evolutionary Biology 2023Quote: Protein was separated by electrophoresis on 12% SDSCPAGE and transferred to a PVDF (polyvinylidene difluoride) membrane at 100 mA for 2 h using a Mini-PROTEAN Tetra system (Bio-Rad, USA). For detection of PpSerpin1 isoform proteins or PrPAP1 ...
-
bioRxiv - Physiology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes using a semi-dry transfer cell (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% non-fat dry milk in TBS-Tween for 1 h at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... using a Laemmli buffer system and then semidry transferred to nitrocellulose membranes by a Trans-Blot Turbo Transfer System (Bio-Rad, USA). The membranes were blocked with 5% bovine serum albumin in PBS at room temperature for 1 h and incubated overnight at 4 °C with specific anti-phospho-eNOS (Ser1177) ...
-
bioRxiv - Cell Biology 2024Quote: ... 20 μg of protein/well was separated on 12% SDS-PAGE and transferred onto PVDF membranes under semi-dry conditions using the Trans-Blot Turbo Transfer System transfer unit (Bio-Rad, #1704150). Ponceau S staining (Sigma-Aldrich ...
-
bioRxiv - Immunology 2024Quote: ... Equal amounts of proteins were separated via SDS‒PAGE and subsequently transferred onto PVDF membranes using a Trans-blot turbo transfer system (all from Bio-Rad, #1704156). The primary Abs used were as follows ...
-
bioRxiv - Genetics 2024Quote: ... membranes were washed three times with PBST and incubated with the appropriate StarBright Blue Fluorescent secondary antibodies (Bio-Rad 12005866 or 12004161) for 1 hour at room temperature ...
-
bioRxiv - Developmental Biology 2024Quote: ... 50 μg of protein from each sample were separated using SDS-PAGE with 7.5% gels and transferred onto Immun-Blot PVDF membranes (Bio-Rad, 162-0177). Membranes were washed in Tris-buffered saline (TBS ...
-
bioRxiv - Immunology 2024Quote: ... A total of 80 µg of protein homogenate were separated by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad, Hercules, CA). Membranes were blocked with fat-free milk powder (5% w/v ...
-
bioRxiv - Genetics 2024Quote: ... and the RNA lanes were transferred to a positively charged nitrocellulose membrane using a Trans-Blot® TurboTM Transfer System (Bio-Rad) and crosslinked using 120 mJ/cm2 UV radiation ...
-
bioRxiv - Plant Biology 2024Quote: ... the immunoprecipitated and input proteins were separated on SDS–PAGE gels and transferred onto PVDF membranes via a Trans-Blot Turbo Transfer System (Bio-Rad, USA). After blocking the membranes with a solution of 5% skim milk prepared in PBST with 0.1% Tween 20 ...
-
bioRxiv - Cell Biology 2024Quote: ... Protein was resolved using SDS-PAGE and transferred to the nitrocellulose membrane using a transfer system (Trans-Blot Turbo; Bio-Rad Laboratories). The membrane was blocked using 5% skimmed milk (American Bioanalytical ...
-
bioRxiv - Bioengineering 2024Quote: ... The transfer to polyvinylidene difluoride (PVDF) membranes was performed with a Trans-Blot® TurboTM system (at 25 V for 10 min, Bio-Rad). Blocking was conducted for 2 h using Tris-buffered saline (20 mM Tris ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto Trans-Blot® Turbo™ Mini PVDF Transfer membranes with the Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked with 5% skimmed milk in PBS with added Tween20 0.05% (Merck ...
-
bioRxiv - Developmental Biology 2024Quote: ... of total protein in Laemmli Buffer were subjected to electrophoresis on precast polyacrylamide gels and transferred to PVDF membranes (Bio-Rad Laboratories). List of primary antibodies is provided in Supplementary Table 2 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Membranes were incubated with the chemiluminescent substrate and visualized using the ChemiDoc®Gel Documentation System (Bio-Rad Laboratories, Hercules, CA, USA). 21
-
bioRxiv - Microbiology 2024Quote: ... proteins extracted from either murine lung tissues or mammalian respiratory epithelial cells were separated on a 10% SDS-PAGE gel and then transferred onto a PVDF membrane (Bio-Rad, USA). The PVDF membrane was blocked with TBST with 5% BSA for 3 h at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein samples were loaded on 4–15% Criterion™ Tris-HCl Protein Gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Bio-Rad). Nonspecific binding sites were blocked for 1 h at room temperature in 5% nonfat milk in PBS-T ...
-
bioRxiv - Cell Biology 2024Quote: ... was loaded on 10-12% TGX Stain-free SDS-PAGE gel (Bio Rad, 1610183 and 1610185) and transferred to PVDF membrane (Bio-Rad, 1620177). Primary antibodies used in this study were as follows ...
-
bioRxiv - Immunology 2024Quote: ... and the gel was blotted onto a nitrocellulose membrane (Cytiva 10600002) with BioRad Trans-Blot Semi-Dry Transfer Cell (BIO-RAD 1703940) and Tris-Glycine Transfer Buffer (Thermo Scientific 28363 ...
-
bioRxiv - Immunology 2024Quote: ... Equal quantities of proteins were subjected to Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and transferred to Polyvinylidene Fluoride (PVDF) membranes (BIO-RAD, #1620177). Then the membranes were blocked with 5% nonfat milk and incubated with primary antibodies (diluted serum of patients or HERV-K102 Env-TM autoantibodies) ...
-
bioRxiv - Neuroscience 2024Quote: ... The proteins were then transferred from the gel to a nitrocellulose membrane using the Trans-Blot Turbo™ Transfer System (Bio-Rad) at 25V ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... Proteins from total and bound fractions were separated by 7.5 % SDS-PAGE and transferred to polyvinylidene difluoride membranes (Bio-Rad, Hercules, CA). To reduce the background signal ...
-
bioRxiv - Cancer Biology 2024Quote: ... Thirty micrograms of each protein sample were separated by SDS-PAGE and transferred onto a nitrocellulose membrane using the Transblot Turbo Transfer System (Bio-Rad, 1704150EDU). Non-specific binding sites on the membranes were blocked with 5% BSA in TBS-Tween 0.1% for 60 min ...
-
bioRxiv - Microbiology 2024Quote: ... Membranes were blocked in 6% milk in PBS and subsequently stained with mouse anti-V5 (clone SV5-Pk1, BioRad, 1:10 000) and goat anti-mouse (1:100 000 ...
-
bioRxiv - Neuroscience 2024Quote: ... The membranes were washed 5X in 1XTBS-T (0.05%) for 15 min and developed using Clarity western ECL substrate (Bio-Rad 170-5060). Scanning was performed with Bio-Rad fluorescent documentation system ...
-
bioRxiv - Microbiology 2024Quote: ... Membranes were then blocked for 1 hour in 5% non-fat milk in PBS containing 0.1% Tween 20 (Bio-Rad, Hercules, CA) at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated overnight at 4°C in blocking buffer containing 0.05 μg/ml anti-Mono-ADP-Ribose (Bio-Rad, AbD43647 #TZA020), which had been conjugated to BiCatcher2:HRP (Bio-Rad ...