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Citations for Bio-Rad :
8251 - 8300 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... the membranes were rinsed three times in TBS-T for 5 min and the proteins were visualized by enhanced chemiluminescense (BioRad, Clarity 1705061) in a BioRad Universal III Hood with Image Lab software v.6.0.1 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then run on 4%–12% Bis Tris gels and transferred onto nitrocellulose or PVDF membranes using an iBlot or semidry (Life Technologies and Biorad, respectively).
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bioRxiv - Cell Biology 2021Quote: ... The cell lysates were separated by electrophoresis on pre-cast 4-15% polyacrylamide gels and transferred onto nitrocellulose membranes using a Trans-Blot® Semi-Dry transfer cell (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... 20 μl of eluted protein samples were separated on SDS gel (12%) and transferred to PVDF membrane (Immun-Blot® PVDF, Bio-Rad) through semi-wet blotting protocol ...
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bioRxiv - Plant Biology 2021Quote: ... RNAs were then transferred to Hybond-XL nylon membranes (Amersham Biosciences, Little Chalfont, United Kingdom) via a semi-dry transfer cassette (Bio-Rad Laboratories) and were immobilized by a UV-crosslinker (UVP ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins (30 µg from each sample) were separated by SDS-PAGE and immunoblotted and transferred to polyvinyl difluoride (PVDF) membranes (BioRad, 162-0177) according to standard protocols ...
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bioRxiv - Biophysics 2020Quote: ... Separated proteins in gel were electroblotted onto a polyvinylidene difluoride membrane using Trans-Blot Turbo transfer system according to the manufacturer’s instruction (Bio-Rad, Hercules, California). Primary antibodies specific for D1 (PSII) ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were separated by SDS-PAGE under reducing conditions and transferred to polyvinylidene fluoride (PVDF) membrane using the Trans Blot Turbo system (BioRad, Munich, Germany). After blocking with 10% skim milk (232100 ...
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bioRxiv - Bioengineering 2021Quote: ... We developed western blot membranes with enhanced chemiluminescence (ECL) solution and images were captured with a luminescent image analyzer ChemiDoc (Bio-Rad, USA) or Amersham Imagequant 800 (Cytiva ...
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bioRxiv - Bioengineering 2021Quote: ... We developed western blot membranes with enhanced chemiluminescence (ECL) solution and images were captured with a luminescent image analyzer ChemiDoc (Bio-Rad, USA).
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bioRxiv - Microbiology 2021Quote: ... and then proteins in the gel were transferred to PVDF membrane using Trans-Blot Turbo Mini PVDF Transfer Packs (Bio-Rad, USA) and Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was slot blotted onto a Hybond N+ membrane with the help of a dot-blot apparatus (Bio-Rad, 170-6545) and subsequently hybridized with a biotin-labeled (TTAGGG)3 probe synthesized by Sigma ...
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bioRxiv - Genetics 2020Quote: ... Whole-cell extracts were resolved on SDS-PAGE gels and transferred to prewetted PVDF blotting membranes in a Trans-Blot Turbo apparatus (Bio-Rad, USA). The blots were blocked in Tris-buffered saline containing 0.3% Tween 20 and 5% skim milk followed by incubation with HRP-conjugated N-A5-Ab at room temperature for 3 h ...
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bioRxiv - Cancer Biology 2020Quote: ... 50μg of proteins were electrophoresed in 8% or 12% polyacrylamide gel and then transferred to PVDF membranes (Bio-Rad Laboratory, CA, USA) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... 1.5 µg of gDNA were dissolved in 250 µL TE buffer and loaded onto the membrane with the help of a Bio-Dot apparatus (Bio-Rad, 1706545) and a vacuum-pump ...
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bioRxiv - Cancer Biology 2022Quote: ... prestained protein ladder were resolved on 10% acrylamide gels by SDS-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose membrane (BioRad Cat. # 1620112) at 100 volts for 90 min.
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bioRxiv - Cancer Biology 2022Quote: ... His6MBP-F47R 4C/4S E6 and His6MBP-4C/4S E6 were spotted on a 0.45 µm nitrocellulose membrane (BioRad, Cat. # 162-0116) and allowed to dry at room temperature for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... while the other gel was transferred to a nitrocellulose membrane using the Trans-Blot Turbo Transfer system following manufacturer guidelines (Bio-Rad Laboratories). The nitrocellulose membrane was removed and placed in 1X Casein blocker for one hour on rotator followed by incubation with a 1:1000 dilution of his HRP-conjugated antibodies (Proteintech ...
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bioRxiv - Cell Biology 2022Quote: Protein extracts were separated under denaturing conditions by SDS–PAGE and transferred to nitrocellulose membranes using a Trans-Blot Turbo nitrocellulose transfer pack (Bio-Rad, 1704159). Membranes were blocked for 45 min at room temperature using 1x StartingBlock buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were blotted onto 0.45 µm nitrocellulose membrane (Amersham) overnight at a constant current of 200 mA using a Trans-Blot® Cell (Bio-Rad, USA).
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bioRxiv - Microbiology 2022Quote: ... Proteins were run on a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the transfer to a nitrocellulose membrane (Amersham™ Protran®) was performed in a wet system (Transfer chamber mini-Protean, BioRad). Membranes were blocked with 5% low fat powdered milk (Roth ...
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bioRxiv - Microbiology 2022Quote: ... the proteins were separated on a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, USA). The blots were blocked in 5% BSA in Tris-buffered saline with Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... Either 50 μg or 25 μg (puromycin blots only) of total protein was separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620177) for blotting ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the whole cell membrane pellets were resuspended in buffer and protein concentration was estimated by dye binding (Coomassie Brilliant Blue R-250; Bio-Rad, USA). The binding reaction was carried out in a final volume of 0.1 ml of Na+-containing buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: Equal amounts (10 µg) of nuclear proteins from different samples were separated using SDS– PAGE and electrotransferred to a nitrocellulose membrane (BioRad, 162-0115) for 1.5 h at constant 400 mA at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins (30 μg/lane) were separated on an SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad Laboratories). The membrane was immunoblotted for CSF1R (#MAB3291 ...
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bioRxiv - Molecular Biology 2024Quote: ... in MOPS buffer at 100 V for 20 min and subsequently at 150V for 190 min on ice along with PageRuler™ Plus Prestained Protein Ladder (Thermo Scientific. Proteins were transferred to a 0.2 µm nitrocellulose membrane by Trans-Blot Turbo™ Transfer system (Bio-Rad). Membranes were washed with TBS (50mM Tris pH 7.6 ...
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bioRxiv - Genetics 2024Quote: ... The pulled down proteins were separated by Sodium dodecyl sulfate Poly-Acrylamide Gel Electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Bio-Rad Laboratories). Membranes were incubated with anti-ACTB (Proteintech ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein(s) in the gel were transferred to a PVDF membrane using a Mini-PROTEAN Tetra Cell (Bio-Rad, United States). After blocking the membrane with 3% BSA at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... 10% Acrylamide:Bis Acrylamide) for 1 hour and transferred to a nitrocellulose membrane (0.45µm) by semidry transfer (BioRad Trans-Blot SD transfer cell) at a constant current for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of proteins (30µg) were separated by 7.5% SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad, Marnes-la-Coquette, France). After blocking membranes in Tris Buffer Solution with Tween-20 0.1 M (TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed prior to incubation with a goat anti-mouse StarBright™ Blue 700 secondary (Catalogue #: 12004158; Bio-Rad, Hercules, CA). Gag expression levels were normalized relative to GAPDH levels ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were transferred to a PVDF transfer membrane (0.45 μm, ThermoScientific #88518) by using a Trans-Blot®Turbo™ transfer system (Bio-Rad) at a constant current of 2.5A for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by size by running 20 μg of the protein extract in 8% SDS-PAGE and transferred to Immuno-Blot PVDF Membrane (Bio-Rad, 1620174) using the Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: 40 μg of protein lysate was resolved on NuPAGE Novex 4-12 % Bis-Tris gels and transferred onto nitrocellulose membranes (Bio-Rad system). Membranes were blocked with 5 % BSA in TBS-T (10 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysates were separated on SDS/PAGE and proteins on the gels were transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 5% skim milk diluted in TBST for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein lysates were resolved by SDS/polyacrylamide gel electrophoresis (PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (Bio-Rad, Hercules, CA, USA). After blocking ...
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bioRxiv - Immunology 2024Quote: ... Lysates were separated by NuPAGE 4- 12 % Bis-Tris Gels (Thermo Fischer Scientific) and transferred to nitrocellulose membranes (Trans-Blot Turbo Transfer Systems (Bio-Rad Laboratories). Membranes were blocked with Odyssey Blocking Buffer (Li-Cor ...
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bioRxiv - Cell Biology 2024Quote: ... Transfer of the proteins from gel to the Immun-Blot® Low Fluorescence PVDF membrane with low fluorescent transfer buffer (BIORAD; #1704275) was performed in Trans-Blot Turbo Transfer System (BIORAD ...
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bioRxiv - Neuroscience 2023Quote: Proteins were separated on SDS-PAGE under denaturing conditions and transferred to nitrocellulose membrane using a MINI PROTEAN II™ system (Bio-Rad). Membranes were blocked with 5% milk powder/TBS-T and incubated with the primary antibodies overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: The histones containing residue-specific acetylations or the nucleosomes used for the acetyltransferase activity assay were electrophoresed in a 10–20% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) (DRC, NXV-396HP20) and transferred onto a nitrocellulose membrane (BIO-RAD, 1620112) at 20 V for 10 min by the semi-dry method ...
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bioRxiv - Cell Biology 2023Quote: ... Protein from whole cell lysates and conditioned media were analyzed by running samples on SDS/polyacrylamide gel electrophoresis (4-15% Stain-free SDS-PAGE minigels) and transferred to PVDF membrane (Immun-Blot PVDF, Bio-Rad 1620177). Membranes were blocked in 3% BSA in Tris-buffered saline containing 1% Tween-20 (pH7.4) ...
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bioRxiv - Molecular Biology 2023Quote: ... and adjusted samples were subjected to SDS-polyacrylamide gel electrophoresis followed by an overnight transfer onto PVDF membranes (Bio-Rad Laboratories, 1620177). Membranes were immunoblotted with antibodies overnight at 4°C as follows ...
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bioRxiv - Pathology 2023Quote: ... and adjusted samples were subjected to SDS- polyacrylamide gel electrophoresis followed by an overnight transfer onto PVDF membranes (Bio-Rad Laboratories, 1620177). Membranes were immunoblotted with antibodies overnight at 4℃ as follows ...
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was visualised by chemiluminescence on Biorad ChemiDoc MP Imager after incubation with goat anti rabbit HRP conjugate (Biorad; 170-6515) or goat anti-mouse-HRP conjugate (Biorad ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were then separated on 10-12% SDS-PAGE gels and transferred to a polyvinylidene difluoride (PVDF) membrane using the Trans Blot Turbo™ transfer system (Bio-Rad). Membranes were blocked and primary antibodies were diluted in 2% BSA in Tris-buffered saline (TBS) ...
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bioRxiv - Biochemistry 2023Quote: ... Detection was performed with Amersham ECL® PrimeWestern blotting chemiluminescent detection reagent (Cityva) and the membranes were scanned with a ChemiDoc XRS+ imaging system (Bio-Rad) using the ImageLab 6.1 software (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... Detection was performed with Amersham ECL® PrimeWestern blotting chemiluminescent detection reagent (Cityva) and the membrane was scanned with a ChemiDoc XRS+ imaging system (Bio-Rad) using the ImageLab 6.1 software (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... and transferred to polyvinylidene difluoride membranes using a semi-dry Bio-Rad transfer machine (Trans-Blot™ Turbo TM Transfer System) with 1X Transfer Buffer (Bio-Rad) at 25V for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... Five µL of each supernatant were run on a 5% to 20% gradient polyacrylamide gel (Wako) and then transferred onto an Immun-Blot® polyvinylidene fluoride membrane (Bio-Rad). Membranes were blocked with 5% skim milk in PBS containing 0.1% Tween-20 (PBST ...