Labshake search
Citations for Bio-Rad :
8201 - 8250 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed 3 times for 5 min in TBST before detection using Clarity Western ECL Substrate (Bio-rad, 170-5061) and imaging on a ChemiDoc MP (Bio-rad ...
-
bioRxiv - Genomics 2020Quote: ... equal amounts of protein were separated by SDS-PAGE on a 10% acrylamide gel and transferred to PVDF membranes using a Trans-Blot® Turbo machine (Bio-rad) according to manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2021Quote: ... 20 μg of total cell proteins were run in a 12% SDS-polyacrylamide gel and blotted to the PVDF membrane (Bio-Rad, USA). Membranes were incubated overnight at 4 °C with mature BDNF primary antibody (ThermoFisher OSB00017W ...
-
bioRxiv - Molecular Biology 2020Quote: ... for 5 minutes and then transferred onto a protran BA-85 nitrocellulose membrane using a semi-dry transfer apparatus (Biorad 170-3940) for 75min at 400mA (not exceeding 15V) ...
-
bioRxiv - Molecular Biology 2021Quote: 5 μg of cheRNA or total RNA for each condition were denatured at 95°C for 5 min and transferred to a Hybond-XL membrane (Amersham) in a Bio-Dot® Microfiltration Apparatus (Bio-Rad) following manufacturer’s intructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... proteins were transferred to Amersham Protran 0.45 NC nitrocellulose membranes (Cytiva, MA, USA) using a Trans-Blot Turbo Transfer System (BioRad Hercules, CA, USA), blocked in blocking buffer (TBST ...
-
bioRxiv - Cancer Biology 2020Quote: ... The proteins were resolved by SDS-PAGE 12% self-cast gel and transferred onto polyvinylidene difluoride (PVDF) membrane using the Mini Trans-Blot® Electrophoretic Transfer Cell (Bio-Rad) in transfer buffer (25 mM Tris ...
-
Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... Proteins were separated on SDS-containing 8 to 12 % polyacrylamide gels and transferred to PVDF membranes (Bio-Rad Laboratories, Inc., Hercules, USA). After staining with primary and secondary antibodies ...
-
bioRxiv - Biochemistry 2020Quote: ... The proteins were then transferred on a Nitrocellulose membrane for 12 minutes using the semi-dry turbo transfer western blot apparatus (Bio-Rad, USA). Post-transfer ...
-
bioRxiv - Cancer Biology 2021Quote: ... membranes were washed 3x with TBS-T and incubated with either Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, #1721011) or Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Bio-Rad ...
-
bioRxiv - Cancer Biology 2020Quote: ... Each PVDF-membrane piece (per antibody) was separately imaged via the auto-exposure function of the ChemiDoc-MP imaging system (Bio-Rad Laboratories). Supplementary-Fig.S4B legend describes the WB-signal quantification in detail ...
-
bioRxiv - Physiology 2021Quote: ... 10 μg of total protein for each sample was separated by SDS-PAGE and transferred to Immun-Blot PVDF membrane (Bio-Rad Laboratories). Subsequently ...
-
bioRxiv - Biochemistry 2020Quote: ... were separated with 15% SDS-PAGE (polyacrylamide gel electrophoresis) and transferred to a nitrocellulose membrane (Chemie Braunschweig, Cat# MSNC02030301) with a semidry system (BioRad, Cat# 1704150) at a constant amperage of 0.5 A and 25 V for 45 min ...
-
bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant protein extracts were subjected to SDS-PAGE and proteins were transferred to a nitrocellulose membrane according to manufacturer’s instructions (Bio-RAD, Hercules, CA). Protein blots were first probed with GL2-specific antibody ...
-
bioRxiv - Cell Biology 2021Quote: ... 3–8% Tris-acetate gels (Life Technologies. Proteins were transferred to nitrocellulose membrane by semi-dry transfer (Trans-Blot Turbo transfer system; Bio-Rad) or ...
-
bioRxiv - Cell Biology 2021Quote: ... Gels were blotted on 0.45 µm nitrocellulose membrane (Amersham) overnight at a constant current of 200 mA using a Trans-Blot® Cell (Bio-Rad, USA). Membranes were decorated using anti-mCherry-1C51 (Abcam) ...
-
bioRxiv - Plant Biology 2021Quote: ... Transfer to a nitrocellulose membrane was performed by semi-dry blotting with standard Bjerrum Schafer-Nielsen buffer (Bio-Rad Laboratories GmbH, Feldkirchen). Membranes were blocked using TBS-T containing 1 % (w/v ...
-
bioRxiv - Plant Biology 2021Quote: ... The membrane was first probed with the indicated primary antibodies and then incubated with goat anti-rabbit (Bio-Rad, cat. no. 1706515) or goat anti-mouse (Bio-Rad ...
-
Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated samples were separated by SDS-PAGE and transferred onto a polyvinylidene difluoride membrane using Trans-Blot turbo Transfer system (Bio-Rad, Munich), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... The proteins in the gel were transferred to a nitrocellulose membrane by Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories) at 25V ...
-
bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed three times in TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Bio-Rad 1,706,515, 1:10,000) secondary antibodies for 2 h at room temperature ...
-
bioRxiv - Plant Biology 2021Quote: Protein bands were run in a 15% polyacrylamide gel and transferred to a PVDF membrane using a mini trans-blot cell (Bio-Rad, USA). The membrane was stained with Coomassie blue R-250 and the bands were cut with a sterilized scalpel ...
-
bioRxiv - Cell Biology 2021Quote: ... A dot blot vacuum manifold (Schleicher and Schuell Dot-Blot System) was used to pull 50 μl of each sample through onto a 0.45 μm nitrocellulose membrane (Biorad, Cat#162-0115). The membrane was washed 1× with water ...
-
bioRxiv - Cell Biology 2021Quote: Purified proteins or protein extracts were separated under denaturing conditions by SDS–PAGE and transferred to nitrocellulose membrane using Trans-Blot Turbo nitrocellulose transfer pack (Bio-Rad, 1704159). Membranes were blocked for 45 min at room temperature using 1x StartingBlock buffer (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... Equal amounts of cellular proteins were resolved on 10% or 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE) and subsequently transferred to nitrocellulose membranes (Bio-Rad Laboratories). Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA ...
-
bioRxiv - Microbiology 2022Quote: ... or in-house prepared 15% polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad Laboratories). The membranes were incubated in blocking buffer [5% (w/v ...
-
bioRxiv - Biochemistry 2022Quote: Proteins were transferred onto 0.22 μm nitrocellulose membranes with the semi-dry transfer protocol in TurboBlot®-buffer (Bio-Rad Laboratories, Inc.). The membrane was blocked with Tris-buffered saline ...
-
bioRxiv - Cancer Biology 2022Quote: ... Samples were run on denaturing sodium dodecylsulfate-polyacrylamide gel electrophoresis gels (12%) before transferring onto Immuno-Blot™ polyvinylidene fluoride membranes (Bio-Rad). The reference protein β-actin was used as a loading control.
-
bioRxiv - Biochemistry 2022Quote: ... the membrane is washed three times with PBST and incubated with an ECL Western blot reagent (1,705,060, Bio-Rad, Hercules, CA, USA) for 5 min in the dark ...
-
bioRxiv - Neuroscience 2022Quote: Aliquots of protein samples were separated on 12% polyacrylamide gels in Tris-glycine-SDS buffer (SDS-PAGE) and electronically transferred to nitrocellulose membranes (GE, 10600001, USA) using a semi-dry blotting system (Bio-Rad, USA). Membranes were blocked at room temperature (RT ...
-
bioRxiv - Neuroscience 2022Quote: ... 1:4 & 1:8) and loaded in triplicates onto a nitrocellulose membrane assembled in a 96-well dot-blot apparatus (Biorad, Cat#1703938). The membrane was then stained with Ponceau (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2022Quote: ... and biotinylated hFLO media were loaded into 16% SDS-PAGE gel and transferred onto 0.2 μm nitrocellulose membranes (1620150, Bio-Rad, CA, USA) through electro blotting ...
-
bioRxiv - Microbiology 2020Quote: ... using AnykD or 12% polyacrylamide gels and transferred to PVDF membranes using the Transblot Turbo apparatus (Bio-Rad, Hercules, CA, United States). PVDF membranes were blocked for 1-hour in TBST with 5% milk ...
-
bioRxiv - Plant Biology 2021Quote: ... Afterwards protein bands were transferred to a nitrocellulose membrane using a transfer apparatus according to the manufacturer’s protocols (Bio-Rad, Hercules, CA). After incubation with 5% nonfat milk in TBST (50mM Tris-Cl ...
-
bioRxiv - Neuroscience 2021Quote: ... 40μg proteins were loaded and separated by SDS-PAGE gels (10%) electrophoretically and transferred to polyvinylidene difluoride membrane (Bio-Rad Incorporation, 10026933). Membrane was blocked with 5% BSA for 60 min at room temperature on shaker ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were then run on 4%–12% Bis Tris gels and transferred onto nitrocellulose or PVDF membranes using an iBlot or semidry (Life Technologies and Biorad, respectively).
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred onto low-fluorescence polyvinylidene fluoride membranes using a semidry transfer method according to the manufacturer’s instructions (Bio-Rad, cat # 1704274). Membranes were blocked in 5% nonfat dry milk before probing with the specific antibodies listed in Supplemental Table 1 ...
-
bioRxiv - Neuroscience 2022Quote: ... After two hours of running, the gels were then transferred onto a nitrocellulose membrane (Amersham, Switzerland) with a semi-dry system (Bio-Rad, Switzerland), and immunoblotting was performed as previously described31,34.
-
bioRxiv - Bioengineering 2022Quote: ... that consists of 1 mL peroxide and 1mL luminol enhancer were spread onto membrane and left for 5 minutes before observation using ChemiDoc imager (Bio-rad Laboratories).
-
bioRxiv - Developmental Biology 2020Quote: ... and separated protein was transferred from gels to polyvinylidene fluoride membranes (PVDF) using a Trans-Blot Turbo Transfer system (Bio-Rad Laboratories). The membrane was blocked with 5% Bovine Serum Albumin (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2021Quote: ... Following 4x 10 min washes with PBS-T the membrane was incubated for 1 min with Clarity Western ECL Substrate (170-5060, Bio-Rad Laboratories) and imaged with Amersham™ Imager 600 (GE ...
-
bioRxiv - Cancer Biology 2021Quote: Protein (20 µg) was separated electrophoretically on SDS polyacrylamide gels and then transferred to a nitrocellulose membrane (BIO-RAD Laboratories, Mississauga, ON). After blocking ...
-
bioRxiv - Cancer Biology 2020Quote: ... The protein samples were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE) and transferred to a nitrocellulose membrane (Bio-Rad, Canada). The expression levels of ABCB1 ...
-
bioRxiv - Microbiology 2020Quote: ... Forty μg of total proteins were separated by electrophoresis on 10% polyacrylamide gels and transferred to PVDF membranes (Bio-Rad, CA, USA). The membranes were blocked with 5% BSA in Tris-buffered saline for 1 h at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... Replicate gels were stained with Coomassie Blue R 250 or transblotted onto Immobilon polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories, Hercules, CA), reacted with affinity-purified YidC1 or YidC2 C-terminal-specific polyclonal rabbit antibodies (1:1000 ...
-
bioRxiv - Microbiology 2020Quote: ... The final hydrophobin preparation was concentrated by ultrafiltration (YM1 membrane; Amicon) and the solvent changed to water with gel filtration (Biogel P6-DG; Bio-Rad, USA). An aliquot was subjected to SDS-PAGE (15% gel) ...
-
bioRxiv - Microbiology 2021Quote: ... The separated protein was transferred to a nitrocellulose membrane in Towbin buffer 1× using the Mini Trans-Blot system (Biorad, United States) at 120mA for 120 minutes in ice cold conditions ...
-
bioRxiv - Immunology 2021Quote: ... Secondary antibodies were added according to manufacturer’s protocol and membranes were imaged using chemiluminescence reagent Clarity Western ECL Substrate (BioRad laboratories, Hercules, CA) and a BioRad ChemiDoc XRS system ...
-
bioRxiv - Bioengineering 2020Quote: ... The membranes were developed using ECL western blotting substrate (Pierce Biotechnology, Rockford, IL) and scanned by ChemiDoc XRS+ system (BioRad, Hercules, CA). Western blots were quantified using Image Lab 6.0.1 software (Bio-Rad Laboratories ...
-
bioRxiv - Genetics 2021Quote: ... A total of 20 μg protein was separated with 12% (vol/vol) sodium dodecyl sulphate (SDS)-polyacrylamide gel and electrophoretically transferred onto a polyvinylidene difluoride membrane (Bio-Rad, L1620177). The membrane was soaked with 5% (w/v ...