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Citations for Bio-Rad :
8001 - 8050 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Protein samples were electrophoresed on home-made 4-16% Tris-glycine SDS-polyacrylamide gels and transferred to nitrocellulose membranes (0.45 µm, Bio-Rad, 1620115). Membranes were blocked in 5% non-fat milk ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 µg of total protein was resolved on 10% SDS-PAGE gels followed by immunoblotting on PVDF membranes (Bio-rad). Membranes were developed by chemiluminescence (SuperSignal West Pico ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were heated at 95°C for 5 minutes and cooled to room temperature before being separated on an 8% tris-glycine SDS-PAGE gel and transferred to a nitrocellulose membrane using a semi-dry transfer machine (Bio-Rad). Membranes were blocked in 5% skim milk dissolved in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Immunology 2023Quote: ... of each sample were resolved by SDS-PAGE and transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked with PBS ...
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bioRxiv - Microbiology 2023Quote: Total protein extracts (5×106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVDF Membranes (Bio-Rad). Immunodetection was performed using the primary antibodies ...
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Modifications in the T arm of tRNA globally determine tRNA maturation, function and cellular fitnessbioRxiv - Molecular Biology 2023Quote: ... RNA was transferred to a nylon membrane (Amersham Hybond-XL) at 23 V for 7 minutes using a Trans-Blot Turbo Transfer System (Bio-Rad). Following transfer ...
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bioRxiv - Cancer Biology 2023Quote: ... and the proteins were transferred to nitrocellulose membranes using a Turbo-Blot semidry transfer apparatus from Bio-Rad (Hercules, CA, USA). Immunoblotting was performed by incubating the membranes with 5% milk or bovine serum albumin for 60 minutes ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were resolved on 7-15% gradient polyacrylamide (SDS-PAGE) gels and transferred to nitrocellulose membranes via Trans Blot Turbo (Bio-Rad). Membranes were then blocked with 5% milk in Tris-buffered saline and 0.05% Tween 20 (TBST ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins (10–30 μg) were run on a 10% SDS–polyacrylamide gels and then transferred to a nitrocellulose membrane (Biorad®). The membranes were blocked in 5% lowfat milk-PBS-tween solution and then incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... PVDF membranes were washed three times with tween 20-containing tris buffered saline before incubation with HRP-conjugated secondary antibodies (Bio-Rad). Images were detected using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with TBS-T and chemiluminescence was assessed with the BioRad ChemiDoc MP Imaging System (BioRad, 12003154).
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bioRxiv - Cell Biology 2023Quote: ... and transferred to a nitrocellulose membrane (Maine Manufacturing, LLC) using a Trans-Blot SD Semi-Dry Transfer Cell apparatus (Bio-Rad). Primary antibodies were a rabbit monoclonal anti-HA (Cell Signaling ...
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bioRxiv - Cell Biology 2023Quote: ... 40-80 μg protein was separated on SDS-PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (#162–0177; Bio-Rad). Membranes were blocked in phosphate buffered saline (PBS)-Tween with 5% non-fat dry milk ...
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bioRxiv - Cell Biology 2023Quote: ... 30 μg of total protein per sample was subjected to reducing and denaturing SDS-PAGE prior to Western transfer onto nitrocellulose membrane (Bio-Rad). After blocking with 5% skim milk in PBS containing 0.1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein lysates (20 µg) were separated in 10% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). The membranes were incubated with specific primary antibodies (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... the membranes were developed with chemistry high-sig ECL Western Blotting substrate(Tanon) and analyzed with the chemiDoc XR (BIO-RAD).
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bioRxiv - Neuroscience 2023Quote: ... An equal amount of sample was loaded and separated on a 12% bis-tris gel and transferred to PVDF membranes (1620177 Bio-Rad) using a Bio-Rad cassette at 70 volts for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... proteins (20 μg per lane) were separated by SDS–polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Bio-Rad). Membranes were blocked in 4% milk in TBST [0.01 M tris (pH7.4) ...
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bioRxiv - Cell Biology 2023Quote: All the protein samples were separated by sodium dodecyl sulfate polyacrylamide (SDS)-PAGE and were transferred to nitrocellulose membrane (Bio-Rad laboratories ...
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bioRxiv - Microbiology 2023Quote: Cell and virion lysates were boiled and analyzed by Western blotting using a nitrocellulose membrane and wet transfer (350 mA, 75 min, Bio-Rad). Western blots were developed with 1:2500 goat anti-gp120 polyclonal Ab (Invitrogen) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed 3 times with TBST for 5 min each and then the membrane was incubated with Clarity Western ECL Substrate (Bio-Rad) for 2 min before exposure to film and development in a darkroom.
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then incubated with respective primary and secondary antibodies before image acquisition using the ChemiDoc MP imaging system (Bio-Rad). Densitometry analysis was conducted using ImageJ and protein expression levels were normalized to α-tubulin loading control.
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bioRxiv - Neuroscience 2024Quote: ... 1:5000 dilution in 10% milk) the membranes were imaged as described above but using Clarity Max ECL Western Blotting Substrate (BioRad, 1705062).
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bioRxiv - Neuroscience 2024Quote: ... Each gel was transferred to a methanol-activated Immobilon-FL PVDF membrane (MilliporeSigma) which was then UV-activated and imaged for total protein content on a Chemidoc MP (Bio-Rad). Membranes were blocked at room temperature for 1 hr in a 1:1 mixture of PBS and fluorescent blocking buffer (Rockland Immunochemicals ...
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bioRxiv - Cancer Biology 2024Quote: ... ten micrograms of proteins were separated by SDS-PAGE and transferred onto 0.2 μm PVDF membranes (Bio-Rad Laboratories, Inc., 1704156) using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2024Quote: Cell lysates were resolved on 7.5 or 4–15% Mini-PROTEAN and Criterion TGX SDS- PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes using a Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were revealed using the ChemiDoc Imaging System Bio-Rad and the signals quantified with the Image Lab software (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... The membrane was then washed with TBS-T and chemiluminescence was assessed with the BioRad ChemiDoc MP Imaging System (BioRad, 12003154).
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bioRxiv - Microbiology 2024Quote: ... and 1.5 µl H2O2 and applied to the membrane before the ECL signal was detected with a ChemiDoc XRS imaging system (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... Samples were then transferred to a PVDF membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad, Cat # 1704150). Membranes were blocked using 5% non-fat milk powder diluted in TBST (Tris-buffered Saline ...
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bioRxiv - Immunology 2024Quote: ... Proteins lysates were denatured with DTT and boiled prior to separation by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad). Primary antibody incubations (Supplementary Table 2 ...
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bioRxiv - Microbiology 2024Quote: Total RNA was loaded onto 8 to 12% PAGE–7 M urea gels and transferred to Hybond-N+ (GE) membranes using a Trans-Blot Turbo Transfer system (Bio-Rad) in 1X TBE ...
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bioRxiv - Molecular Biology 2024Quote: ... 500ng of RNA has been diluted in 250 μl of TE low EDTA buffer and applied to pre-equilibrated positively charged nylon membrane (Amersham Hybond-N+ # RPN203B) on Slot blot apparatus (Biorad # 1706542) by gentle vacuum ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by SDS-PAGE on 16% acrylamide gels immobilized by transfer to a PVDF membrane (Cat. #1620177, Bio-Rad) using a Trans-Blot Turbo Transfer System (Trans-Blot ®TurboTM ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were split and electrotransferred using 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels and the PVDF membrane Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed 3 times for 10 min with TBS-T and developed with an enhanced chemiluminescence (ECL Bio-Rad 1705061) substrate ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred on a PVDF membrane in a Mini-Protean Tetra System apparatus or a Trans-Blot Turbo Transfer System (Bio-Rad). Abundant epitopes were blocked with a buffer containing 5% milk or ...
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bioRxiv - Physiology 2024Quote: ... Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane using a wet transfer cell (Bio-Rad) at 100 mAmps (4°C overnight) ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Membranes were then washed three times with and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred from the gel onto a nitrocellulose membrane using a semi-dry method (BioRad Trans-Blot Turbo). The membrane was blocked with 5% non-fat dry milk in 1X TBST for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: The protein extracts were separated by 10% SDS-PAGE and the proteins were transferred on the low fluorescence PVDF membrane (Bio-rad). The molecular weight of the proteins was assigned by Precision Plus Protein Dual Color Standards (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were mixed with 3x laemmli buffer and resolved by SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad # 1620177) for immune blotting ...
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bioRxiv - Microbiology 2024Quote: ... The gels were subsequently transferred for 10 min onto a polyvinylidene difluoride membrane (PVDF) using the Trans-Blot turbo transfer system (Bio-rad). The membranes were blocked using blocking buffer prepared using 5% milk (Alkali Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated on 10 % polyacrylamide gels and blotted onto a nitrocellulose membrane at 100 V for 1 h using the Mini Trans-Blot system (Bio-Rad). Membranes were blocked with 5 % milk powder in PTw (1x PBS ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred to PVDF difluoride membranes in Trans-Blot Turbo transfer buffer in a Trans-Blot Turbo transfer system (Bio-Rad) as per the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were then transferred to nitrocellulose membranes using Trans-Blot® Turbo (TM Transfer System, Biorad, catalog no.170-01918). Membranes were blocked using 1x TBST (0.2 mM Tris Base pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were resolved in 1x Laemelli reducing buffer by SDS-PAGE electrophoresis and transferred to a Nitrocellulose blotting membrane (Bio-Rad). Subsequently ...
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bioRxiv - Plant Biology 2024Quote: Proteins were resolved on a 10% sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel and blotted onto polyvinylidene fluoride (PVDF) membrane using the Trans-blot turbo transfer system (Bio-Rad). PVDF membranes were incubated with anti-HA (1:5,000 ...