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Citations for Bio-Rad :
7851 - 7900 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: The purified baculoviruses were electrophoresed on 10% sodium dodecyl sulfate-polyacrylamide gel (BIOTOOLS) and transferred to a low-fluorescence polyvinylidene fluoride (PVDF) membrane (Bio-Rad) through Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... and 110 V for additional 2.5-3 h) and transferred to a nitrocellulose membrane overnight (~16 h) at 15 V using the Bio-Rad Transblot (Bio-Rad). Membranes were blocked in 5% fat-free milk in Tris buffered saline with Tween-20 (TBST ...
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bioRxiv - Cell Biology 2022Quote: ... was applied directly to the membrane for band detection and imaged using Bio-Rad ChemiDoc XRS+ System (Bio-Rad Laboratories, RRID:SCR_008426). Image analysis and densitometry of protein bands were performed using Image Lab software (v6.0.1 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were heated in reducing Laemmli sample buffer at 95°C and resolved in SDS-PAGE protein gel and transferred to PVDF membrane (Bio-Rad). Membranes were blocked using 5% BSA (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed in the same conditions than previously and the detection was performed with Clarity Western ECL Substrate (Bio-Rad). The following antibodies were used for western blot ...
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bioRxiv - Cell Biology 2022Quote: ... separated into an 8%-12% SDS-PAGE gels and transferred to a nitrocellulose Hybond-C membranes using a Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked with 5% milk in PBS-0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Lysed samples were separated on a SDS (sodium dodecyl sulfate) polyacrylamide gel and blotted onto a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein was resolved on 10% SDS–polyacrylamide gel electrophoresis gels and transferred onto PVDF membranes (Bio-Rad, catalog no. 1704156) by Trans-Blot Turbo (Bio-rad) ...
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bioRxiv - Genomics 2022Quote: ... and 20 ug of each sample were loaded on a 6% or 10% SDS-PAGE gel and transferred to a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad). Western Blot was blocked using 5% ELK and incubated overnight with primary antibody mouse monoclonal anti-UHRF1 (Santa Cruz ...
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bioRxiv - Plant Biology 2022Quote: ... 0.01% [v/v] β-mercaptoethanol) was separated by 10% SDS-PAGE gel and blotted onto Immun-Blot PVDF membrane (Bio-Rad). D1 ...
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bioRxiv - Microbiology 2022Quote: ... then transferred to a PVDF membrane using Turbo-Blot transfer apparatus and Trans-Blot TurboTM Midi PVDF Transfer Packs (Bio-Rad). The membrane was blocked with PBS + 5% milk for 1 hour at room temperature then incubated with a PBS + 1% milk solution containing a 1:2000 polyclonal anti-DnaA antibody (Eurogentec ...
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bioRxiv - Neuroscience 2022Quote: ... Samples (20 μg total protein in 200 μL) were spotted onto a nitrocellulose membrane using a vacuum-assisted dot blot apparatus (Bio-Dot Apparatus 1706545, Bio-Rad). Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200 ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed for 5 min 3x with TBS-T and incubated with secondary goat anti-rabbit-HRP antibody (Bio-Rad) at a 1:10000 ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked by incubating in 15 mL of blocking solution (5% Blotting Grade Blocker Non-Fat Dry Milk (Bio-Rad) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... Equal amount in µg from each the supernatant was loaded on a 10% (w/v) SDS-PAGE gel and transferred to a PVDF membrane (Bio-Rad). Puromycin incorporation was measured using an anti-puromycin antibody (Merk Millipore).
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bioRxiv - Microbiology 2022Quote: LTA extracts (10 μl) were run on 15 % polyacrylamide gels and transferred to PVDF membranes using a Trans-Blot Turbo transfer system (Bio-Rad). After blocking with 5 % milk and 1 % BSA in TBST ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by electrophoresis at a constant 130 V/gel for 90 min and transferred to a nitrocellulose membrane on the Trans-Blot Turbo Transfer System (Bio-Rad) at 25 V for 7 min ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with beta-mercaptoethanol were boiled for 5 min and separated on 12% SDS-PAGE gels prior to transfer to methanol-activated PVDF membranes using the Trans-Blot Turbo system (Bio-Rad). Membranes were blocked in Tris-buffered saline with Tween-20 (TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... DNA/protein complexes were transferred to a Hybond-N+ positively charged nylon membrane (Amersham Pharmacia Biotech) using an electroblot transfer system (Bio-Rad) and cross-linked with short-wave UV light in a GS gene linker UV chamber (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... were separated on 12% (w/v) SDS-PAGE gels and transferred to an Immun-Blot low fluorescence-PVDF membrane (Bio-rad). The membrane was first blocked with Odyssey Blocking Buffer (TBS ...
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bioRxiv - Systems Biology 2022Quote: ... semidry electrotransfer of proteins to a PVDF membrane was carried out in a Trans-Blot Turbo Transfer System (Bio-Rad, USA). The membrane was incubated in blocking buffer (PBST ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred onto Immun-Blot PVDF membrane (BioRab, 1620177) and incubated overnight in blocking solution (5% powdered milk, NaF in TBST [TBS (BioRad, 1706435) 1% Tween] ...
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bioRxiv - Neuroscience 2022Quote: ... 200 µg of protein extracts were loaded and separated on a 10% SDS-PAGE gels and transferred onto PVDF membranes (Bio-Rad). Membranes were blocked with a 3% BSA in TBS-T (Tris-Buffered Saline with Tween-20 containing in mM ...
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bioRxiv - Molecular Biology 2023Quote: Kidney and liver tissue were homogenized in RIPA and were analysed by SDS-Page and Immuno-blotting on PVDF membranes as by the manufacturer protocol (Trans-Blot Turbo Transfer System, Bio-Rad). Incubation with primary antibodies (Supp ...
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bioRxiv - Neuroscience 2023Quote: ... The pellets were resuspended with 2 % SDS and loaded onto a cellulose acetate membrane assembled in a slot blot apparatus (Bio-Rad). The membrane was washed with 0.2% SDS and retained SDS-insoluble mHTT aggregates were detected with mouse anti-polyQ antibody (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed and then incubated with the secondary antibody (Goat Anti-Rabbit IgG – HRP conjugate, Bio-Rad cat# 1706515) for one hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed 5 X 5 min in TBST and signals were detected using the Clarity Western ECL Substrate (Biorad #1705060). At least 2 separate gels were immunoblotted with cortical extracts from independent litters and used for quantitation.
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bioRxiv - Microbiology 2023Quote: ... separated on a 10% SDS-PAGE and transferred to a PVDF membrane using the Trans-Blot® Turbo™ system (BioRad). His-tagged proteins were visualised using a primary anti-6xhis antibody (ArtNr ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to a nitrocellulose membrane using the High Molecular Weight protocol on the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked in 5% milk in 0.05% Tween 20 in PBS for 1h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Protein (30 μg) was resolved on a 12% SDS-PAGE and transferred to a 0.45 μm nitrocellulose membrane (BioRad Cat# 1620115). The membranes were probed with anti-MAG ...
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bioRxiv - Physiology 2022Quote: ... after which the separated protein was transferred to a polyvinylidene fluoride (PVDF) membrane in a Trans-Blot Turbo System (Bio-Rad) at 25 V (1.3 A ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 60 μg of each protein sample was loaded on the polyacrylamide gel and then transferred to polyvinylidenedifluoride (PVDF) membrane (TransBlot Turbo, Bio-Rad) with semi-dry transfer (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... Gels were blotted on nitrocellulose membranes and transfer was carried out by using the “high molecular weight” program of the Trans-Blot Turbo System (Bio-Rad). The membranes were blocked in a blocking solution (5% skim milk ...
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bioRxiv - Bioengineering 2022Quote: ... membranes were washed again in PBS-Tween before being placed in 0.1 mL working solution (equal parts stable peroxide solution and Luminol/Enhancer solution) per cm2 membrane for 5 minutes before being placed for imaging in ChemiDoc Gel Imaging System (Bio-Rad). Gels were quantified using image J by taking the ratio of the integrated intensity (area x intensity ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting lysate was resolved on SDS-PAGE gel and protein was blotted to nitrocellulose membrane (Li-COR #926-31902) with mini Trans-Blot Cell (Bio-Rad). Membranes were blocked for an hour in Odyssey Blocking Buffer (TBS ...
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated with anti-mouse or –rabbit secondary antibodies tagged with horseradish peroxidase (1/10 000, Bio-Rad Laboratories) for one hour at room temperature and visualized by chemiluminescence (Amersham ECL prime western blotting detection reagent ...
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bioRxiv - Molecular Biology 2022Quote: ... Immunoreactivity was detected using West-Q Pico Dura ECL Solution (Catalog number W3653, GenDEPOT) and membranes were imaged on a ChemiDoc XRS+ Imaging system (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by electrophoresis at a constant 130 V/gel for 90 min and transferred to a nitrocellulose membrane on the Trans-Blot Turbo Transfer System (Bio-Rad) at 25 V for 7 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 15-30 μg of protein lysate was loaded and separated on 12% Tris-glycine SDS-PAGE gels and transferred to nitrocellulose membranes (catalog number 1620167, Bio-Rad) using Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Bioengineering 2022Quote: ... Equal amounts of protein extracts were resolved by 10%-12% SDS-PAGE and electrotransferred onto a polyvinylidene membrane (Bio-Rad, USA). After blocking in Tris-buffered saline plus 5% (w/v ...
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bioRxiv - Bioengineering 2022Quote: ... SDS page was performed on the samples and the proteins were transferred to nitrocellulose membranes using wet transfer (Bio-Rad Laboratories). The membranes were blocked with PBS with 0.1% Tween-20 (PBST ...
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bioRxiv - Cell Biology 2022Quote: ... The gel was transferred to a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad Inc., Mississauga, ON, Canada) at 1.3 A and 2.5 V for 7 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Microgram of total proteins were separated by SDS-PAGE and transferred on to PVDF membranes using a semi-dry electroblotting apparatus (TransBlot Turbo, BioRad, Germany). Blotted membranes were then blocked with 5% (w/v ...
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bioRxiv - Biophysics 2024Quote: ... total proteins and biotinylated proteins were separated by 12% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). The Signal Enhancer HIKARI (Nacalai Tesque ...
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bioRxiv - Synthetic Biology 2024Quote: The samples (1 µL) were subjected to SDS-PAGE and transferred to a nitro cellulose membrane (Bio-Rad Laboratories, CA, USA). The membrane was blocked using 3% skim milk in PBST (137 mM NaCl ...
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bioRxiv - Physiology 2024Quote: ... was used to wash the membranes before incubating with goat anti-rabbit horseradish peroxidase conjugated secondary antibody at 1:5000 dilution (1706515, Bio-Rad) for 90 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 µg proteins were analyzed through a 4-15% gradient SDS-PAGE before being transferred to the PVDF membrane with the TurboBlot system (Bio-Rad). The membrane was blocked with 5% (w/v ...
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bioRxiv - Plant Biology 2024Quote: Proteins from SDS-PAGE gels were transferred onto a PVDF (polyvinylidene difluoride) membrane (pre-activated in methanol for 1 min) using Trans-Blot Turbo transfer system (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Equal amounts of protein lysates (20 µg) were separated in 10% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). The membranes were incubated with specific primary antibodies (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... or immunoprecipitated samples were resolved by SDS-PAGE (6% to 10%) and transferred onto methanol-treated immun-blot PVDF membrane (Bio-Rad). The membranes were incubated overnight at 4°C with corresponding primary antibodies (antibody information ...