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Citations for Bio-Rad :
7701 - 7750 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... secondary antibodies were incubated with the PVDF membrane for 1 hour at room temperature (Goat anti-rabbit antibody, BioRad, 1:3000). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... gels were transferred to 0.45 μm polyvinylidene difluoride membranes (cat. no. IPFL00010, MilliporeSigma) using the Criterion blotter and Towbin buffer (cat. no. 1704070 and 1610771, Bio-Rad) at 10 V overnight in a cold room ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes (Amersham Protran) for 30 min at 15V with a Trans-Blot SD semidry transfer cell (Bio-Rad) or in transfer buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... loaded on SDS-polyacrylamide gels (8 to 10 %) and transferred to nitrocellulose membranes using Trans-blot turbo transfer system (Bio-Rad). Membranes were saturated for 1 h with TBST buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: Following electrophoretic separation, oligonucleotide-protein complexes were transferred by electro-blotting (330 mA, 1 h) onto specific positively charged nylon membranes (Bio-Rad) and detected using an anti-digoxigenin antibody conjugated with alkaline phosphatase.
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto a pre-activated PVDF membrane using the Trans-Blot Turbo transfer system (BIO-RAD). Membranes were blocked with 5% non-fat milk in tris-buffered saline with Tween-20 (TBST ...
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred to PVDF membranes (Trans-blot Turbo Transfer Pack) using the Trans-blot Turbo Transfer System (both from Bio-Rad) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Recombinant human GFAP protein were run on 12% SDS-PAGE and transferred onto membrane via Trans-Blot Turbo Transfer System (BIO-RAD). Membrane was incubated in blocking buffer (PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... were separated by SDS PAGE in 4-12% Bolt Bis-Tris Plus gels (ThermoFischer Scientific) and transferred to 0.2 µm Immun-Blot® PVDF membrane (BIO-RAD). After blocking with 5% (w/v ...
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bioRxiv - Bioengineering 2020Quote: ... of the of the cell lysate were loaded and separated by SDS–PAGE before transfer to a nitrocellulose membrane (BioRad, #1620177). Membranes were incubated with primary mouse anti-EGFR antibody (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were resolved on polyacrylamide gels by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred electrophoretically to a nitrocellulose membrane (Bio-Rad) at 100V for 100 min ...
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bioRxiv - Microbiology 2020Quote: ... The gel was blotted on a PVDF membrane using a semi-dry transfer unit (Trans-Blot Semi-Dry Electrophotetic transfer cell, Bio-Rad) at 25V for 45 minutes ...
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bioRxiv - Immunology 2021Quote: ... Lysates were collected and equal protein amounts were separated by SDS-PAGE and blotted onto a nitrocellulose membrane by wet-blotting (Bio-Rad). Membranes were blocked with TBS-T containing 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated with LDS buffer and reducing agent before running on an SDS-PAGE gel and transferred onto a PVDF membrane (Bio-Rad). The membranes were blocked and then probed using target-specific antibodies ...
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bioRxiv - Microbiology 2020Quote: ... cell lysates were resolved on 7.5% Mini-PROTEAN TGX SDS-PAGE gels and then transferred to polyvinylidene difluoride (PVDF) membranes using a Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% (w/v ...
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bioRxiv - Systems Biology 2021Quote: ... each supernatant sample was run in duplicate and volumetric band intensities were fitted onto a standard curve generated by a dilution series of HPC4-tagged EPO on the same membrane using the Image Lab software (Bio-Rad).
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bioRxiv - Plant Biology 2020Quote: ... of total protein for each sample were separated by SDS-PAGE (10% acrylamide gel) and transferred to a PVDF membrane (Bio-Rad). The membrane was blocked in TBST buffer containing 5% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... The membranes were then incubated with secondary antibodies (1:10000, goat anti-rabbit or goat anti-mouse IgG HRP conjugate (Bio-Rad) for 1 hour and detection was performed using the Immobilon-luminol reagent assay (EMP Millipore).
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were subjected to SDS-PAGE and the proteins in the gel were transferred to a PBDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad). After probing the membrane with the primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated using SDS-polyacrylamide gel electrophoresis (SDS-PAGE-4-12%) and transblotted onto nitrocellulose membranes (Bio-rad, California, USA). The membranes were blocked with 5% dry milk (overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Only 300µL of the 1mL final volume was directly applied into the system in a nitrocellulose membrane (Bio-Rad, CA, USA).
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bioRxiv - Neuroscience 2021Quote: ... All samples were boiled at 65°C for 10 minutes and then equal amounts of each sample were separated using SDS-PAGE and subsequently blotted on nitrocellulose membranes using the Trans-Blot Turbo System (Bio-Rad). Membranes were washed in Tris-buffered saline-Tween (TBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... 40 μg of total protein for each sample was resolved by SDS-PAGE prior to transfer to nitrocellulose membranes (Bio-Rad). Membranes were blocked in Tris-buffered saline with+ 0.1% (v/v ...
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bioRxiv - Biochemistry 2021Quote: The cell lysate was separated by 12.5% (w/v) SDS-PAGE electrophoresis (ATTO Cooperation, Japan) and transferred onto a PVDF membrane (Bio-rad, USA). The membrane blots were blocked in 1x EzBlockChemi (ATTO Cooperation ...
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bioRxiv - Biochemistry 2020Quote: Cell lysates were heated at 95°C for 5 min in Laemmli Sample Buffer and then subjected to sodium-dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE; (52)) followed by electrophoretic transfer to 0.45 μm nitrocellulose membranes (Bio-Rad; 1620115). Membranes were blocked in PBS-0.05% Tween20 (PBST ...
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bioRxiv - Neuroscience 2021Quote: ... 40 min) and were subsequently transferred to nitrocellulose membranes for semi-dry immunoblotting (25 V, 1,3 A, 7 min, Bio-Rad®). Membranes were dried 5 min at 37°C and then incubated with 5% non-fat dried milk in Tris-buffered salineTween-20® (TBST containing 28 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: Samples from the synaptosomal fractionation were resolved by SDS-PAGE and transferred to a 0.2 μm nitrocellulose membrane (Bio-Rad Laboratories). After blocking non-specific binding with 5% nonfat milk in 1X TBST (Tris-Buffered Saline ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed again 3 times for 20 minutes and then developed using the BioRad imaging system (BioRad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 min and proteins were resolved on 10% SDS polyacrylamide gels and blotted onto nitrocellulose membranes (Bio-Rad). Anti-rabbit Phospho-p44/42 MAPK antibody (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were separated on a 12% Bis-Tris polyacrylamide gel and blotted onto a PVDF membrane by using the iblot apparatus (Bio-rad). Membranes were blocked with 5% top-block in PBS-T followed by incubation with POM1 mouse IgG1 antibody (200 ng ml−1) ...
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bioRxiv - Neuroscience 2020Quote: ... were separated in a 10% acrylamide gel by SDS-PAGE and transferred to a nitrocellulose membrane according to the manufacturers protocols (Bio-Rad). Following a 60min incubation in 5% non-fat milk in TBS-T the membrane was incubated with primary and secondary antibodies (Supplementary Tables 3 ...
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bioRxiv - Cell Biology 2021Quote: ... according to their molecular weight and transferred to a nitrocellulose Hybond-C membrane using an iBlot Gel Transfer Device (Bio-Rad) or using a wet-transfer system (if protein molecular weight >120kDa) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 to 30μg of total protein were subjected to 8% or 10% SDS-PAGE gels and transferred electrophoretically onto PVDF membrane by a semi-dry blotting system (Bio-Rad). The membrane was blocked in 5% fat-free milk/Tris-buffered saline for 1 hour and incubated with primary antibodies at 4°C overnight followed by horseradish peroxidase conjugated secondary antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto a polyvinylidene diflouride (PVDF) membrane using a Trans-Blot turbo transfer system (Bio-Rad). The membrane was blocked with 5% non-fat dried milk powder in Tris-buffered saline and 1% Tween 20 and probed with appropriate antisera ...
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bioRxiv - Systems Biology 2020Quote: ... following the incubation of the membranes with the appropriate HRP-conjugated secondary antibodies and the Clarity Western ECL Substrate (Bio-Rad). The antibodies used in this study are ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μg per lane of total protein samples were separated on 4-12% Bolt Bis-Tris Plus Gels and blotted onto polyvinylidene difluoride membrane (Bio-Rad) using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... ECL was performed and the membrane was exposed and quantified in a Bio-Rad ChemiDocXRS Imaging System (Bio-Rad, Hercules, CA).
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bioRxiv - Zoology 2021Quote: ... These standardized data were then normalized to total protein from the same membranes using a SYPRO Ruby Blot Stain (Bio-Rad). The averages of phosphorylated MAPK/total MAPK/total protein in the hearts from treatment fish ...
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bioRxiv - Genetics 2021Quote: ... The 13 μl of protein was run on a 10% SDS-PAGE gel and transferred to a PDVF membrane by semidry transfer (Bio-Rad) at 13V for 90 min ...
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bioRxiv - Microbiology 2021Quote: ... A 30µg protein extracts were subjected to denaturing 10% SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membrane preactivated with absolute methanol using Trans-Blot Turbo Transfer System (Bio-Rad). After blocking with 5% powdered skim milk in 1% phosphate buffer solution plus 0.1% Tween-20 (PBST) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated by 12% SDS-PAGE and stained with Coomassie or transferred to nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked in 10% skimmed milk in TBS-Tween 0.05% (TBS-T) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to a PDVF membrane for 1 hour at 100V and blocked for 1 hour (5% blotting-grade blocker (Bio-Rad) in PBS containing 0.05% Tween 20) ...
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bioRxiv - Microbiology 2020Quote: ... resolved on Novex 4–20% tris–glycine gels and transferred to nitrocellulose membranes using the Trans-Blot Turbo Transfer system (Bio-Rad). As a loading control ...
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bioRxiv - Microbiology 2020Quote: ... Protein lysates from approximately 2.5 x106 parasites were subjected to electrophoresis on 7.5% SDS-PAGE gels and transferred onto 0.45 µm nitrocellulose membranes (Bio-Rad, cat. # 1620115). Buffer containing a high concentration of both Tris and glycine (50 mM Tris ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated via SDS-PAGE and transferred to a PVDF membrane using Trans-Blot Turbo System (Bio-rad). The following antibodies were used for detection ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were subjected to enhanced chemiluminescence (ECL)-based detection and cytokine signals were visualized using ChemiDoc touch imaging system (BioRad, California).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with PBS 1X/0.1% Tween 20 and incubated with Clarity™ Western Enhanced chemiluminescence (ECL) Substrate (BIO-RAD), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: Extracellular medium and cell lysates were spotted on nitrocellulose membranes (0.45 μm) using Bio-Dot Microfiltration Apparatus (Bio-Rad, California, USA) (manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated on 12% or 15 % SDS-PAGE gels and transferred onto PVDF membranes using a Trans-Blot Turbo transfer system (Bio-Rad). Blocking was performed with 3% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed 3 times for 5 min and incubated with the secondary antibodies (1:10000, IgG-HRP, Bio-Rad) for 45 min at RT ...