Labshake search
Citations for Bio-Rad :
7901 - 7950 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... the proteins were blotted onto methanol-activated polyvinylidene difluoride (PVDF) membranes (0.2 µm) with a Trans-Blot SD Semi-Dry Transfer Cell system (Bio-Rad Laboratories). After blotting ...
-
bioRxiv - Neuroscience 2024Quote: ... Membranes transferred from TGX Stain-Free™ gels were imaged and quantified for posterior normalization with a ChemiDoc XRS+ (Bio-Rad) using the Image Lab software (Bio-Rad) ...
-
bioRxiv - Microbiology 2024Quote: Total RNA was loaded onto 8 to 12% PAGE–7 M urea gels and transferred to Hybond-N+ (GE) membranes using a Trans-Blot Turbo Transfer system (Bio-Rad) in 1X TBE ...
-
bioRxiv - Neuroscience 2024Quote: ... The samples were separated on a 15% Tris-Glycine gel with a 4% stacker using a Tris/Glycine/SDS buffer and transferred overnight onto an Immobilon-FL Transfer Membrane using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked for one hour (0.5% Non-Fat Dry Milk in PBS ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein samples were loaded for electrophoresis in 5% or 15% polyacrylamide gels containing sodium dodecyl sulfate (SDS‒PAGE) and transferred onto nitrocellulose membranes using the Trans-Blot Turbo system (Bio-Rad). The membranes were incubated with the primary antibodies listed in Supplemental Table S1 at 4 °C overnight after blocking with Blocking One reagent (Nacalai Tesque) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.5% of total input and 15% of total IP fraction were resolved on 10% SDS-PAGE gels and wet transferred onto a 0.45 µm nitrocellulose membrane for 2 h at 80 V and 4°C (Bio-Rad, Germany). The membrane was blocked for 1 h in 5% BSA in PBST (PBS + 0.2% Tween20) ...
-
bioRxiv - Microbiology 2024Quote: ... the membrane was developed using the ECL chemiluminescence detection system (Beyotime P0018FS) and visualized with the ChemiDoc XRS+ system (Bio-Rad). Three independent experiments were conducted for reproducibility.
-
bioRxiv - Microbiology 2023Quote: ... run on a 12% SDS‒PAGE gel and transferred onto a PVDF membrane using a Trans-Blot Turbo electrophoretic transfer cell (Bio-Rad) at 25 V for 30 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... and subsequently transferred to polyvinylidene difluoride (PVDF) membrane using (Trans-Blot® Turbo™ Mini PVDF Transfer Pack, Bio-Rad # 1704156). The membranes were often cut into strips and the individual strips were hybridized with indicated primary antibodies overnight at 4°C followed by incubation with respective horse radish peroxidase conjugates ...
-
bioRxiv - Biophysics 2024Quote: ... the membrane was incubated with an HPR-conjugated secondary antibody and visualized with enhanced chemiluminescence using ChemiDoc Imaging System (Bio-Rad). Signal intensities were measured in ImageLab Software ...
-
bioRxiv - Plant Biology 2023Quote: ... The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF) membrane (Bio-Rad). All the antibodies were obtained from commercial suppliers (anti-GFP and anti-MYC antibodies from Roche ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein transfer to the nitrocellulose membrane was carried out by rapid transfer: the transfer sandwich containing the gel and membrane was placed into a Trans-Blot Turbo Transfer System (Biorad, USA), which was then run at 25 V for 9 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... An equal quantity of protein for the respective samples were resolved by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad). The membrane was blocked with 5% nonfat dry milk ...
-
bioRxiv - Cancer Biology 2023Quote: ... Nitrocellulose membranes were blocked for 1h at RT in 5% Blotting Grade Blocker Non-Fat Dry Milk (Biorad, Hercules, CA, USA) and TBST ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 50 µg of the membrane protein eluate fraction quantified by BSA were diluted to 32 μl in lysis buffer containing loading Laemmli buffer (Bio-Rad) supplemented with 10% 2-mercaptoethanol ...
-
bioRxiv - Developmental Biology 2023Quote: ... Beads were collected after washing thrice with 1× RIPA buffer and separated on a 12% denaturing SDS polyacrylamide gel followed by transfer onto Immuno-blot PVDF membranes (Bio-Rad). Mouse anti-arm antibody at 1:1500 dilution or rabbit anti-FLAG antibody at 1:1000 dilution and goat anti-rabbit IgG–AP conjugate at 1:2000 or goat anti-mouse IgG-AP conjugate at 1:200 dilution (Molecular Probes ...
-
bioRxiv - Biophysics 2024Quote: ... the membrane was incubated with an HPR-conjugated secondary antibody and visualized with enhanced chemiluminescence using ChemiDoc Imaging System (Bio-Rad).
-
bioRxiv - Developmental Biology 2024Quote: ... miRNAs were separated in 15% denaturing urea polyacrylamide gel in 1X TBE and then were transferred to a Zeta-Probe blotting membrane (Bio-Rad) using a semi-dry Trans-Blot SD (Bio-Rad ...
-
bioRxiv - Cell Biology 2024Quote: The proteins were separated on polyacrylamide gels and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad). The membranes were blocked with 5% NFDM in TBST (50 mM Tris/HCl pH 8 ...
-
bioRxiv - Cancer Biology 2024Quote: ... was used for developing the signal and membranes were imaged and analyzed using the ChemiDoc Imaging System with ImageLab software (Bio-Rad). Cell lysates from the p53 mutant Eµ-Myc lymphoma cell line (EMRK1172 ...
-
bioRxiv - Biochemistry 2024Quote: ... The proteins in the gel were transferred to a polyvinylidene difluoride membrane (Immobilon-P) using a semidry transfer apparatus (Amersham Life Sciences or Trans Blot Turbo Transfer System from Bio-Rad). Membranes were blocked using PBS containing 2% BSA and 0.05% Tween-20 ...
-
bioRxiv - Cell Biology 2023Quote: ... proteins incubated with Mal-B were electrotransferred to PVDF membranes using a Trans-blot SD semi-dry transfer cell (Bio-Rad). Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v ...
-
bioRxiv - Genetics 2024Quote: ... Forty micrograms of whole-cell lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Bio-Rad). The membranes were blocked with 5% nonfat dry milk in Tris-buffered saline with 0.05% Tween 20 (TBST ...
-
bioRxiv - Immunology 2024Quote: ... the proteins were transferred to a polyvinylidene difluoride membrane (110V, 1h, 4°C) using a Mini Trans-Blot cell apparatus (Bio-Rad). Non-specific binding sites were blocked using PBS/Tween 20 (0.05% Tween 20 ...
-
bioRxiv - Immunology 2024Quote: ... It was then transferred to a PVDF membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad). Membranes were blocked with Tris-buffered saline with Tween20 (TBST ...
-
bioRxiv - Biochemistry 2023Quote: ... Approximately 20-30 μg of protein was resolved on 8% polyacrylamide gels and transferred onto nitrocellulose membranes using semi-dry Trans-Blot TurboTM (Bio-Rad). Following antibody incubation (anti-GFP ...
-
bioRxiv - Cell Biology 2023Quote: ... were added with 2X Laemmli buffer and were resolved on SDS-PAGE followed by their transfer on methanol charged Polyvinylidene fluoride (PVDF) membrane using semidry transfer system (Biorad, USA). Membrane was further treated using 5% skimmed milk in PBST for 1 hour and then probed with primary antibody and incubated overnight at 4[ ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were separated by SDS-PAGE electrophoresis in 12% or 14% acrylamide gels, and transferred on nitrocellulose membranes (Amersham, 10600021) by semi-dry electrophoretic transfer (Bio-Rad). Accordingly with primary antibody datasheets ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins transferred from SDS-polyacrylamide gels to nitrocellulose membranes were visualized using a ChemiDoc analysis system (Bio-Rad, Hercules, CA, USA). Several exposures were analyzed to determine the linear range of the chemiluminescence signals ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated on 10% or 4-20% CRITERION TGX gels under reducing conditions and transferred to Immun-Blot PVDF membranes (Bio-Rad). The membranes were blocked with 5% BSA or 5% nonfat milk in TBS-T (0.1% Tween20 ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were run on SDS-PAGE gel and immunoblotted onto a nitrocellulose membrane using Trans-Blot Turbo transfer system (Bio-Rad). Anti-LC3B antibody (anti-rabbit ...
-
bioRxiv - Cancer Biology 2024Quote: ... The samples were then transferred to a PVDF membrane at 400mA for 2 hours at 4°C in 1x Tris/Glycine buffer (1610771, Bio-Rad). Membranes were then blocked in casein blocking buffer (PI37528 ...
-
bioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were separated by SDS-PAGE electrophoresis followed by transference in polyvinylidene fluoride membrane in a Trans-Blot Turbo Transfer System (Bio-Rad). Blotting buffer contained 25 mM Tris ...
-
bioRxiv - Neuroscience 2023Quote: ... samples were transferred to 0.2 μm (for LC3) or 0.45 μm (for everything else) PVDF membranes (BioRad, 162-0175 and 1704275), which were blocked in 5% BSA in PBS at RT for 1 hr ...
-
bioRxiv - Immunology 2023Quote: ... equal amounts of protein (10–40 μg) were loaded into 10-20% SDS–PAGE gradient gels and transferred onto 0.22 μM PVDF membranes (Bio-Rad, 1620177). After air drying to return to a hydrophobic state ...
-
bioRxiv - Plant Biology 2023Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Virus pellets and clarified cell lysates were then analyzed by Western blotting using a nitrocellulose membrane and wet transfer (350 A, 75 min, Bio-Rad). Western blots were developed with 1:2,000 goat anti-gp120 polyclonal antibody (Invitrogen) ...
-
bioRxiv - Cell Biology 2023Quote: ... 30 µg protein extract was loaded onto a 15% Bis-Tris polyacrylamide gel for electrophoresis then transfer to a PVDF membrane using a Mini Trans Blot Cell (Bio-Rad). Membranes were blocked for 1 h at 23°C in 4% milk/PBST (0.1% Tween-20 in PBS) ...
-
bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred from the unstained gel to polyvinylidene fluoride membranes using the Trans-Blot turbo transfer system (Bio-Rad Laboratories). An iBind™ Flex system (Invitrogen/ThermoFischer ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were resolved on 4–20% FastPAGE precast gels (Tsingke Biotechnology) and transferred to nitrocellulose membranes in a Trans-Blot Turbo machine (Bio-Rad). Membranes were blocked with 5% nonfat milk dissolved in Tris-buffered saline (TBS ...
-
bioRxiv - Cell Biology 2023Quote: ... Approximately equal loads of extracted protein were subjected to SDS-PAGE and transferred to a 0.2 µm nitrocellulose membrane (Bio-Rad 1620112). Transferred proteins were then stained with Ponceau S Solution (Sigma-Aldrich P3504) ...
-
bioRxiv - Neuroscience 2023Quote: ... 1 μg of either α-syn PFFs or monomers (biotinylated or untagged) were applied in spots on a nitrocellulose membrane (Cat#1620145, BioRad) for 1h at room temperature (RT) ...
-
bioRxiv - Developmental Biology 2022Quote: Protein extracts were separated on a 12% SDS gel with a 5% stacking gel and transferred to PVDF membranes using the Trans-Blot Turbo System (Bio-Rad). Membranes were blocked using 5% milk for 1 h at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were then transferred to a nitrocellulose membrane (GE Water and Process Technologies) using a wet transfer protocol and a Mini Trans-Blot Cell (Bio-Rad) transfer apparatus at 4C ...
-
bioRxiv - Cell Biology 2023Quote: ... Denatured lysates were analyzed by SDS-PAGE and gels were transferred to PVDF membranes using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked in 5% non-fat dry milk in TBS containing 0.1% Tween-20 (TBST ...
-
bioRxiv - Microbiology 2023Quote: ... 15 μl of lysate were run in 16% SDS-PAGE followed by transfer on the PVDF membrane using TransBlot Turbo device (Bio-Rad). Membrane was washed in mQ and incubated at +4°C overnight in 10 mL of PBS with 5% non-fat dry milk ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS‒PAGE in 12% acrylamide gels and then transferred onto PVDF membranes using a Trans-Blot Turbo Transfer System (Bio-Rad) and PVDF transfer packs (Bio-Rad) ...
-
bioRxiv - Microbiology 2023Quote: ... The gel was run at 125 V for 3 to 4 hours and the RNA was then transferred to a Zeta-Probe GT membrane (Bio-Rad) by capillary action with 20 x SSC buffer (3 M NaCl ...
-
bioRxiv - Neuroscience 2023Quote: ... 15 µg of protein from each sample were subjected to SDS-polyacrylamide gel electrophoresis (PAGE) (12%) and transferred to polyvinylidene difluoride (PVDF) membranes (Bio-Rad) for 90 min ...