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Citations for Bio-Rad :
7951 - 8000 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Five micrograms of total protein were resolved by SDS-PAGE on a 10% gel under reducing conditions and transferred to PVDF membranes (Bio-Rad). The blots were blocked in TBS containing 5% non-fat milk for 30 mins and incubated with SARS-CoV-2 nucleocapsid protein antibody (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... 2% formaldehyde gel and separated at 125-150V at 4°C for 1-2 h and then transferred to a Zeta-Probe GT membrane (Bio-Rad) via capillary action overnight (Streit et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately equal loads of extracted protein was then resolved on tris-glycine 10% SDS-PAGE gels and transferred using wet transfer to 0.2 µm nitrocellulose membrane (Bio-Rad 1620112). Transferred proteins were then stained with Ponceau S Solution (Sigma-Aldrich P3504 ...
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bioRxiv - Molecular Biology 2023Quote: ... boiled in sample buffer and loaded onto a 10% TGX Stain-free FastCast gel and transferred onto a nitrocellulose membrane and enhanced chemiluminescence using Clarity Western ECL substrate (Bio-Rad) was used to visualize the protein.
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bioRxiv - Cell Biology 2023Quote: ... separated by SDS-PAGE using the appropriate Bio-Rad apparatus and transferred to PVDF membranes using the Trans-Blot Turbo transfer system (Bio-Rad). The blots were blocked with 5% BSA in TBST and blotted according to manufacturer’s recommendations for the indicated primary and secondary antibodies (Table S2 ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was wrapped in saran wrap and exposed to a gel documentation system equipped with a CCD camera (BioRad, USA) to capture the signal.
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bioRxiv - Microbiology 2023Quote: ... of serial twofold dilutions of the MV samples were applied to nitrocellulose membranes using a 96-well Bio-dot apparatus (Bio-Rad). Immunodetection of MntC or LTA was performed as described previously (26).
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bioRxiv - Cancer Biology 2023Quote: ... After blocking with 5% (w/v) BSA or milk the membrane was incubated with the corresponding primary and secondary antibodies (Bio-Rad). The specific bands were analyzed using Thyphoon or ChemiDoc Imaging Systems (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: Proteins from lysed cells or immunoprecipitates were denatured and loaded on sodium dodecyl sulfate polyacrylamide gels and then transferred to polyvinylidene difluoride membranes (Bio-Rad). After blocking with 5% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... then 20 μg of protein was resolved on a 4-12% Tris gel and transferred to a polyvinylidene difluoride (PVDF) membrane using the Trans-Blot Turbo system (Bio-Rad) according to standard protocols ...
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bioRxiv - Microbiology 2023Quote: ... 20 µg of total RNA in loading buffer containing 50% formamide and 20% formaldehyde was separated on a formaldehyde agarose gel and subsequently transferred to a Zeta probe nylon membrane (Bio-Rad) by capillarity blotting ...
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bioRxiv - Immunology 2023Quote: ... the eluted samples were dot-blotted and UV cross-linked to a nitrocellulose membrane that was immunoblotted with anti-BrdU (BioRad #AHP2405), anti-8OHdG (Thermo Fisher #BS-1278R ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and wet-transferred overnight at 150 mA to Hybond nitrocellulose membranes (Bio-Rad) as described (125 ...
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bioRxiv - Genetics 2023Quote: ... The gels were run until proper band separation was achieved and the protein was transferred onto a 0.2 µm nitrocellulose membrane using the wet-transfer method (Bio-Rad Laboratories). The membranes were blocked with Odyssey Blocking Buffer (LiCOR Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Total protein was transferred from a 10% SDS-PAGE gel onto a nitrocellulose membrane using the Trans-Blot turbo transfer system (Bio-Rad). Total Flag-sakA was detected using the Flag M2 antibody 1:2000 (Sigma ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were blocked with 4% bovine serum albumin (BSA) (Gemini Bio, 700-109P) dissolved in Tris-buffered saline (Bio-Rad #1706435) supplemented with 0.025% Triton X-100 (TBST) ...
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bioRxiv - Pathology 2023Quote: ... total proteins were extracted and examined as previously described.59 The blotting was performed on the PVDF membrane (Bio-Rad, USA) and imaged with the Western Blotting Detection Kit (Advansta ...
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bioRxiv - Cell Biology 2023Quote: ... Denatured proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and blotted onto a PVDF membrane in a semidry Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked for 1 hour at room temperature with 5% BSA in PBS containing 0.18% Tween-20 (PBS-Tw) ...
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bioRxiv - Cancer Biology 2023Quote: ... Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis and transfers of proteins to .45uM nitrocellulose membranes were performed using the Protean 2 or 3 systems (Bio-Rad) and protocols provided by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred onto PVDF membranes applying the Trans-Blot Turbo Transfer system (Bio-Rad Laboratories). Membranes were blocked with 5% BSA or 5% NFDM in 1X TBS-T buffer and incubated with specific primary and secondary antibodies ...
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bioRxiv - Genomics 2023Quote: ... from cell preparations were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred to a PVDF membrane (Bio-Rad) using a Trans-Blot Turbo™ transfer system (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... 8 µg protein per condition were separated by SDS-PAGE and blotted onto a 0.2 µm nitrocellulose membrane (Bio-Rad, #1704158) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... The protein extract (40μg) was separated on 8 or 10% polyacrylamide mini gel and transferred to a PVDF membrane (Bio-Rad, USA). Blotted membranes were incubated overnight at 4°C in SuperBlock(PBS ...
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bioRxiv - Microbiology 2023Quote: Samples were separated by SDS-PAGE as described previously22 and transferred to nitrocellulose membranes using the TransBlot Turbo System (Bio-Rad) according to manufacturer instructions (25 V ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein lysates were run on 10% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and transferred to a nitrocellulose membrane (Bio-Rad) using a wet tank blotting system ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then spun for 1 min at 20k x g and run on a 10% SDS gel before transferring to nitrocellulose membranes (Bio-Rad) and blocking as described above ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 μg of total protein was resolved on 10% SDS-PAGE gels followed by immunoblotting on PVDF membranes (Bio-rad). Membranes were developed by chemiluminescence (SuperSignal West Pico ...
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bioRxiv - Neuroscience 2022Quote: ... and then subjected to SDS polyacrylamide gel electrophoresis (PAGE) with 8% polyacrylamide and transferred to polyvinylidene difluoride (PVDF) membrane (BioRad Laboratories). The membranes were blocked for 1 h in Tris buffered saline with Tween 20 (TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... and afterward transferred to polyvinylidene difluoride (PVDF) membrane using (Trans-Blot® Turbo™ Mini PVDF Transfer Pack, Bio-Rad # 1704156). After blocking the membranes with 5% blotto (G-Biosciences Blot-Quikblocker # 786-011 ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were transferred onto PVDF membranes (activated in methanol for 1 minute) using the Trans-Blot® TurboTM Transfer System (BioRad). Transferred membranes were washed in tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a Trans-Blot Turbo Mini 0.2 µm Nitrocellulose membrane in a Trans-Blot Turbo Transfer System (Bio-rad: 1704150). Membranes were blocked in Odyssey® Blocking Buffer overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were subjected to 10–12% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... equal amounts of protein lysates resolved by SDS-PAGE (Cat. #4561033; Bio- Rad Laboratories, Hercules, CA, USA) and transferred onto nitrocellulose membranes (Cat. #170-4158; Bio-Rad). Membranes were then blocked with blocking buffer (Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5% Triton X-10 in Sterile MilliQ water) and resolved on 10% SDS-PAGE gels followed by western blotting on Immun-Blot PVDF membranes (Bio-rad). Antibodies used were diluted in 5% milk (Labscientific CN ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were resolved by SDS-PAGE followed by transfer onto nitrocellulose membranes using the Trans-Blot Turbo Western blotting system (Bio-Rad). Blots were probed with FLAG (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... and serial dilutions of F-actin or G-actin were blotted on nitrocellulose membrane using Bio-Dot microfiltration apparatus (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Lysates were separated by sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) and transferred onto nitrocellulose membranes (Bio-Rad Laboratories, US) using a semidry technique (semidry blotter ...
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bioRxiv - Systems Biology 2023Quote: ... Whole-cell lysates were separated on an SDS-PAGE gradient gel (4–15%) and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad). p21 and Cdk2 were detected in 15 μg of whole-cell lysates ...
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bioRxiv - Microbiology 2023Quote: ... equivalent total protein quantities from membrane samples were resolved by SDS-PAGE using Any kDTM polyacrylamide gels (Bio-Rad, Hercules, CA). Proteins transferred to a nitrocellulose membrane were probed with a monoclonal anti-His6 antibody (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: Cell lysates were collected and separated by SDS-PAGE and through electrophoresis were transferred onto a PVDF (Polyvinylidene difluoride) membrane (BIO-RAD). The membranes were blocked with 5% skim milk in TBS with 0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified RNA was separate on a 1.2% agarose-formaldehyde denaturing gel and was then transferred to a nylon membrane (Hybond-N+, Cytvia) in 10X SSC buffer using a model 785 vacuum blotter (Bio-Rad). RNA was cross-linked to the membrane using an ultraviolet (UV ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole-cell lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene fluoride membrane (Bio-Rad). The blots were incubated with anti-CCTα (D18B6 ...
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bioRxiv - Molecular Biology 2023Quote: ... Gels were then transferred onto 0.2 µm nitrocellulose membranes using Trans-Blot Turbo Transfer System gel (Bio-Rad, Hercules, CA, USA). Membranes were incubated overnight at 4°C in Odyssey blocking buffer containing 0.1% Tween-20 at 4°C and 1/1000 dilution of anti-c-myc mouse monoclonal antibody (Catalogue number 11 667 149 007 ...
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bioRxiv - Biochemistry 2023Quote: Proteins were resolved by sodium dodecyl sulfate poly(acrylamide) gel electrophoresis (SDS/PAGE) and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline containing Tween 20 (TBST ...
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bioRxiv - Cell Biology 2023Quote: ... then supernatants of samples were collected and loaded onto gels and transferred onto polyvinylidene fluoride membrane (Trans-Blot Semi-Dry Transfer Cell; Bio-Rad). Membranes were blocked in 2%BSA/TBS + 0.05% Tween-20 and incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Extracts were run on 10% SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus following the standard protocols (Bio-Rad). After incubation with 5% nonfat milk in TBST (10 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: Whole cell lysates and eluates from immunoprecipitations were run on 10% SDS-polyacrylamide gels and transferred to supported nitrocellulose membrane (Bio-Rad) by standard methods ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were then transferred to a 0.22 µm PVDF membrane by blotting at 24 V for 35 min using a Trans-Blot TurboTM system (BioRad, Feldkirchen, Germany). The membrane containing the proteins was blocked in 5% BSA in TBST at RT for 1 h to avoid unspecific binding of the antibodies ...