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Citations for Bio-Rad :
7751 - 7800 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Western blotting was performed according to the manufacturer’s instructions with minor modifications (SDS-PAGE run at 110 V, transfer onto PVDF membrane for 1 h at 110 V (BIO-RAD)) ...
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bioRxiv - Neuroscience 2022Quote: ... 20 μg of total protein were resolved in 4-20% SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo transfer system (Bio-rad). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... 80 µg of whole cell lysate was electrophoresed in a 12 % NuPAGE gel and blotted to PVDF membrane overnight at 24 V using a wet transfer blotting module (Bio-Rad). The blotted membrane was blocked in 5 % skimmed milk ...
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bioRxiv - Plant Biology 2022Quote: ... RNA electrophoresis was performed at 200 V for 1 h and then RNA was transferred to a nylon membrane using MiniProtean 3 system (BioRad, USA). Transfer conditions were 100 V for 1 h at 4 °C in TBE buffer 1 × ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were transferred to nitrocellulose membranes in a Turbo blotter (Trans-Blot Turbo Blotting System, 170-4155, Bio-Rad Laboratories). The total protein amount was visualized using the stain-free image of the blot (Figure 1B) ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 10 µg protein was then separated by SDS/PAGE and transferred to Immuno-Blot PVDF membranes (Bio-Rad). Membranes were incubated in 5% non-fat milk for 1 hour at room temperature to block nonspecific binding ...
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bioRxiv - Microbiology 2022Quote: ... and transferred on a nitrocellulose membrane using a semi-dry Trans-Blot Turbo Transfer System and Trans-Blot Turbo Transfer Buffer (Biorad, USA). Membranes were blocked for 1 hour with 5% milk in TBST (Tris-buffered saline and Tween 20 ...
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bioRxiv - Genetics 2022Quote: ... The 13 µl of protein was run on a 10% SDS-PAGE gel and transferred to a PDVF membrane by semidry transfer (Bio-Rad) at 13V for 2 hours 15 min ...
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bioRxiv - Genetics 2022Quote: ... The entire sample was loaded on 1.5 mm 8% SDS-PAGE gel and ran at 85 volts for 2 hours and transferred to PDVF membrane via semidry transfer (Bio-Rad) at 19 V for 2 hours and 30 minutes ...
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bioRxiv - Genetics 2022Quote: ... The samples were boiled at 65°C for 10 minutes and then loaded on 10% SDS-PAGE gel and ran at 90 volts for 2 hours and transferred to a PDVF membrane via semidry transfer (Bio-Rad) at 13 V for 3 hours ...
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bioRxiv - Neuroscience 2022Quote: ... and transferred to polyvinylidene difluoride membranes using a semi-dry Biorad transfer machine (Trans-Blot® Turbo TM Transfer System) with the 1X Transfer Buffer (BioRad) at 25 V for 20 min ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before detection using Clarity Western ECL Substrate (Bio-Rad 170-5061) and imaging on a ChemiDoc MP (Bio-Rad 17001402).
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bioRxiv - Biochemistry 2022Quote: ... 0.2 μm nitrocellulose membranes were prewet for 10 minutes in TBS then secured into the Bio-Dot Microfiltration apparatus (Bio-Rad). Wells were rehydrated once with TBS ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were separated on ExpressPlus™ PAGE Ready gels 4 - 20% (A2S, M42015) and transferred on the cellulose membrane (300 mA, 90 min) using a BioRad blotting device (BioRad, USA). After overnight blocking in 5% milk in TBST ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins from each cell line were separated through SDS-PAGE (10% resolving gel) and electroblotted onto a polyvinylidene fluoride (PVDF) membrane using the semi-dry blotting system (Bio-rad). Next ...
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bioRxiv - Neuroscience 2021Quote: ... Equivalent protein samples (cell lysates and DRG fractionized membrane and cytosol) were size separated using 10% or 4-20% SDS-PAGE gels (Bio-Rad), transferred to Immun-Blot PVDF membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: Total protein extracts (5 × 106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVFD Membranes (Bio-Rad) [55] ...
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bioRxiv - Microbiology 2020Quote: ... Cellular protein lysates were resolved on Criterion or 4%–20% Bio-Rad precast SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). Membranes were saturated and proteins were blotted with antibodies in 5% non-fat dry milk ...
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bioRxiv - Neuroscience 2020Quote: ... Gels were run using MES [2-(N-morpholino)ethane sulfonic acid] running buffer at 150-160 V (110 V for the first 15 min) and then were electro-transferred to nitrocellulose membrane (Bio-Rad), submerged in transfer buffer (20% methanol ...
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A Phytochrome B-PIF4-MYC2 Module Tunes Secondary Cell Wall Thickening in Response to Shaded LightingbioRxiv - Plant Biology 2021Quote: Proteins extracted from seedling samples were separated with 10% (w/v) SDS-PAGE gels and blotted onto polyvinylidene fluoride membrane (Bio-rad). Protein blots were then analyzed using either anti-GFP/Myc (1:2000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Each sample (30 µg) was run on a 14% SDS-PAGE gel and transferred to a nitrocellulose membrane (Bio-Rad, CA). HTLV-1 Gag expression in cells and VLPs in the cell culture supernatant was detected using a mouse monoclonal antibody HTLV-1 p24 (6G9 ...
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bioRxiv - Immunology 2020Quote: ... Lysates (20–30 μL) were run on 8–10% polyacrylamide gels and transferred to PVDF membranes (Trans-Blot Turbo Transfer System, Bio-Rad). Membranes were blocked with 5% BSA in TBS (TBS ...
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bioRxiv - Cell Biology 2021Quote: Extracellular medium and cell lysates were spotted on nitrocellulose membranes (0.45 μm) using Bio-Dot Microfiltration Apparatus (Bio-Rad, California, USA) (manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Proteins (10 μg or 20 μg) were separated on 12% SDS PAGE gels and were then transferred to activated PVDF membrane (Bio-Rad). Following 2 h of blocking in 5% skimmed milk ...
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bioRxiv - Biophysics 2021Quote: ... and eletro-transfer at 100V for 2h were performed to separate protein extracts and transfer them to PVDF membranes (Bio-Rad). Non-specific sites were blocked with 5% BSA in TBS 0.05% Tween 20 ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... then separated via SDS-PAGE and transferred onto a PVDF membrane (18 min, 15 V) by Trans-Blot® SD semi-dry Western blot (BioRad). The membrane was exposed as described before overnight to a PhosphoImager screen ...
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bioRxiv - Microbiology 2021Quote: ... and transferred on a nitrocellulose membrane using a semi-dry Trans-Blot Turbo Transfer System and Trans-Blot Turbo Transfer Buffer (Biorad, USA). Membranes were blocked for 1 hour with 5% milk in TBST (Tris-Buffered Saline and Tween 20 ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies and later analyzed using a ChemiDoc™ Imaging System (Bio-Rad). Data is presented as ratio of phosphorylated/total protein normalized internally to each uninfected condition.
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane using preassembled Trans-Blot Turbo Transfer Packs and a Trans-Blot Turbo Transfer System (Bio-Rad). The voltage was set to 15 V and samples were run for 7 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 20 μg of protein was separated using a 3–8% Criterion™ XT tris-acetate or a 4–15% Criterion™ XT tris-glycine protein gels and then transferred to PVDF membrane with a Trans-Blot Turbo Transfert system (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... gels were transferred to a polyvinylidene difluoride (PVDF) membrane using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories, Hercules, CA). Membranes were then blocked in TBS-T (50 m mol/L Tris ...
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bioRxiv - Immunology 2022Quote: ... Proteins in culture supernatants and cell lysates were separated by SDS-PAGE and transferred onto PVDF membranes by using the Trans-Blot Turbo system (Bio-rad). Membranes were immunoblotted with anti-caspase-1 ...
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bioRxiv - Genetics 2022Quote: ... then transferred to .45uM nitrocellulose membranes using the 30 minute mixed molecular weight protocol on the Trans-Blot Turbo System from Bio-rad. Membranes were blocked with Intercept (PBS ...
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bioRxiv - Immunology 2022Quote: ... equal amounts of total protein in Laemmli Buffer were subjected to electrophoresis on precast polyacrylamide gels and transferred to PVDF membranes (Bio-Rad). Membranes were blocked and probed overnight with corresponding primary antibodies (MERTK ...
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bioRxiv - Cell Biology 2022Quote: ... and run at room temperature with 100 V (constant) before being transferred to polyvinylidene fluoride or nitrocellulose membranes using the Trans-Blot Turbo system (Bio-Rad) for 10 minutes at 2.5 A ...
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bioRxiv - Cell Biology 2022Quote: ... the proteins were transferred to nitrocellulose membranes using Trans-Blot Turbo Transfer Packs and a Trans-Blot Turbo apparatus/predefined mixed-MW program (Bio-Rad). Blots were blocked with 5% nonfat dry milk (HA blot ...
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bioRxiv - Cell Biology 2022Quote: ... and the fractioned proteins were subsequently transferred electrophoretically onto polyvinylidene fluoride membranes via the Trans-Blot Turbo Transfer System (Bio-Rad). These membranes were blocked in 6% nonfat milk for 1.5 h at room temperature and then probed with antibodies against survivin (cat ...
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bioRxiv - Plant Biology 2020Quote: ... The membrane was incubated with secondary HRP-conjugated antibodies and signals were detected using a chemiluminescent method (ChemiDoc XRS, Bio-Rad).
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bioRxiv - Physiology 2020Quote: ... Transfer of the proteins to the PVDF membranes was performed using the Trans-Blot TurboTM Transfer System (BioRad, Hercules, CA, USA). Membrane was blocked for 1h at RT (5% milk in TBS-T ...
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bioRxiv - Immunology 2020Quote: ... The gels were run for 1 h at 180 V and were then transferred onto PVDF or nitrocellulose membranes using the Trans-Blot TURBO semidry transfer system from Bio-Rad.
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bioRxiv - Synthetic Biology 2020Quote: Samples with equal amount of RNA were applied onto a positively charged membrane using the Bio-Dot Microfiltration Apparatus (Bio-Rad). The membrane was placed on the gasket and wetted with 2x SSC ...
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bioRxiv - Microbiology 2020Quote: ... Samples were resolved by Bolt Bis-Tris Plus 4-12% gels in 1x MES SAS and transferred to PVDF membranes by a Trans-Blot Semi-Dry electrophoretic transfer cell (Bio-Rad) at 20 Volt for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 1:1000)The membranes were then incubated with secondary antibodies (1:10000, goat anti-rabbit or goat anti-mouse IgG HRP conjugate (Bio-Rad) for 1hr and the detection was performed using the immobilon-luminol reagent assay (EMP Millipore).
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bioRxiv - Microbiology 2020Quote: ... The proteins were transferred to a Polyvinylidene difluoride (PVDF) membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad). Detection of GFP was carried out with a rabbit polyclonal anti-GFP antiserum (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... and transferred to PVDF membrane by the standard protocol (25 V, 30 min) of Trans-Blot Turbo Transfer System (Bio-Rad). After blocking with 5% BSA-PBST (137 mM Sodium Chloride ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were subjected to SDS-polyacrylamide gel electrophoresis and proteins were blotted onto nitrocellulose membranes using the Mini Trans-Blot Cell system (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: Total proteins from HepG2 cells were prepared and run on 10% SDS-PAGE and transferred to a PVDF membrane using a transfer apparatus following the standard protocols (Bio-Rad). After incubation with 5% nonfat milk in TBST (10 mM Tris ...
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bioRxiv - Synthetic Biology 2021Quote: ... Total proteins were separated in SDS-PAGE and transferred to a PVDF membrane using Transblot turbo (Bio-Rad Laboratories, CA, USA). After blocking the membrane with 0.3% skim milk in TBS-T ...
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bioRxiv - Immunology 2020Quote: ... 75 μg of total proteins were separated by 15 % SDS–polyacrylamide gel electrophoresis and afterwards transferred onto polyvinylidene difluoride membranes using a transblot transfer chamber (X-Cell SureLock™, Invitrogen Novex Mini-cell, or Mini-PROTEAN Tetra Cell, Biorad). Western blots were probed with anti-MART-1 antibodies (1:1000) ...
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bioRxiv - Microbiology 2020Quote: ... proteins were blotted onto nitrocellulose membranes (Whatman, Maidstone, United Kingdom) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) under conditions suggested by the manufacturer ...