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Citations for Bio-Rad :
7651 - 7700 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a colorimetric assay based on the Bradford dye-binding method (Bio-Rad Protein Assay Dye Reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration in the cell lysate was quantified using the Bradford protein assay (#500-0006, Bio-Rad). 30 μg of each sample was boiled with 4x Laemmli Sample Buffer containing 10% 2-mercaptoethanol (#1610747 ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were transferred to new tubes and total protein was quantified using a Bradford Assay (Bio-Rad). 20 µg protein per sample was reduced with 3 mM TCEP (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The assays were carried out using CFX96 Touch Real-Time PCR System (Bio-Rad, Hercules, California, USA). After an initial denaturation at 95°C for 1 min ...
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bioRxiv - Biochemistry 2024Quote: ... Then the total protein concentration of each soluble fraction was measured by Lowry DC Protein Assay (BioRad). From each soluble fraction ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined using Quick Start™ Bradford Protein Assay (Cat.# 5000201, Bio-Rad Hercules, CA).
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bioRxiv - Microbiology 2024Quote: Cytokines from serum and lung homogenate were quantified using Bio-Plex mouse cytokine 23-plex assay (BioRad) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were collected and analyzed for protein concentration using the Bio-Rad Protein Assay Dye (Bio-Rad). 20-50 mg of protein was denatured at 95°C for 5 min in 5x SDS sample buffer (250 mM Tris-HCl pH 6.8 ...
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bioRxiv - Plant Biology 2024Quote: ... The Supernatant was isolated and sample protein concentrations were calculated by Bradford assay (Bio-Rad Laboratories, 2010) using the Quick Start Bradford 1x Dye Reagent (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... Real-time PCR assays were performed using a CFX Connect Real-Time PCR Detection System (Bio-Rad). Target gene mRNA expression was quantified by real-time PCR using iTaq Universal SYBR Green Supermix (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentrations were determined using the Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, Hercules, CA, USA). The purity was controlled by electrophoresing 3 µg of protein with SDS-sample buffer on a polyacrylamide gel (mPAGE™ 4-20% Bis-Tris Precast Gel ...
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bioRxiv - Immunology 2024Quote: ... Supernatant cytokines were assayed using the Bio-Plex Human Cytokine 17-plex assay system (Biorad, Hercules CA) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... protein concentration of supernatants was determined using the Bio-Rad protein assay (Bio-Rad, Hercules, CA, USA). Supernatants were run with NuPage 4–12% Bis-Tris gels (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the clarified yeast extract was ∼ 80 mg/mL by Bradford assay (BioRad, USA). The extract was stored at - 80 °C until reconstitution experiments were performed.
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bioRxiv - Microbiology 2024Quote: ... The reverse transcription quantitative PCR (RT-qPCR) assays were performed using the CFX96 Real-Time System (BioRad). The primers used in this study are listed in Supplementary Table S2 ...
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bioRxiv - Synthetic Biology 2024Quote: Protein concentrations of lysates for SDS-PAGE were determined using the DC Protein Assay (Bio-Rad, 5000116) against a bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... the supernatant containing soluble proteins was removed and the protein concentration determined via Bradford assay (Bio-rad). Protein concentration was normalised between samples and run on 4-12% bis-tris SDS-PAGE gels (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: Plasma samples was used to perform the Bio-Plex Pro Mouse Diabetes 8-plex assay (Biorad #171F7001M) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentrations were determined using the Bradford method with Bio-Rad protein assay reagent (Bio-Rad, USA) and bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... Reagents for SDS-polyacrylamide gel electrophoresis and Bradford protein assays were from Bio-Rad (Hercules, CA, USA), fetal bovine serum and enhanced chemiluminescence (ECL ...
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bioRxiv - Cancer Biology 2024Quote: ... Concentration of protein collected in the supernatant was determined using DC protein assay (5000113-5; Bio-Rad). Standard procedures were used for western blotting ...
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bioRxiv - Physiology 2024Quote: Real-time polymerase chain reaction (RT-PCR) assay was performed using a MiniOpticon system (Bio-Rad Laboratories) with SYBR Green ER two-step qRT-PCR kit (No ...
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bioRxiv - Cancer Biology 2024Quote: ... The real-time PCR assay was performed in a CFXConnect Real-time PCR detection system (Bio-Rad). One microliter of diluted cDNA was used per reaction ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatants were then collected and protein concentration was determined using the DC Protein Assay Reagent (Biorad). Equal amounts of proteins were resolved on 4-20% Criterion TGX Precast Midi protein gradient gels (Biorad) ...
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bioRxiv - Cancer Biology 2024Quote: ... The real-time PCR assay was conducted in a CFXConnect Real-time PCR detection system (Bio-Rad). One microliter of cDNA was used per reaction ...
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bioRxiv - Biochemistry 2024Quote: ... The total protein content was quantified by Lowry method (DCTM protein assay, Bio-Rad Laboratories, Hercules, USA).
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration of each sample was determined using a colorimetric assay (Bio-Rad, Hercules, CA, USA) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Western blots were detected using chemiluminescence detection kit (Pierce) or chemiluminescence kit (BioRad).
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bioRxiv - Cell Biology 2023Quote: ... kits and cDNA was generated using iScript cDNA Synthesis kits (Bio-Rad #1708890) as per the manufacturer’s specifications ...
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bioRxiv - Microbiology 2023Quote: ... a reverse transcription kit (iScript select cDNA synthesis kit, Bio-Rad, Hercules, CA) was used to create cDNA from the total RNA with gene-specific antisense primers (Table S1) ...
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bioRxiv - Cancer Biology 2021Quote: The prepared whole cell lysates were used to conduct gel electrophoresis followed by transfer to a polyvinylidene fluoride membrane (Bio-Rad). The membrane was blocked in 5% milk solution in Tween-PBS (PBS with 0.1% Tween 20 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with StarBright Blue 700 Goat Anti-Mouse IgG secondary antibody (1:2500, Bio-Rad, Cat# 12004159, RRID: AB_2884948), StarBright Blue 520 Goat Anti-Rabbit IgG secondary antibody (1:2500 ...
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bioRxiv - Microbiology 2020Quote: ... Excess antibody was washed away with 3 x TBST rinses before the membrane was incubated with anti-mouse secondary antibody (Bio-rad) conjugated to horseradish peroxidase at a 1:15000 dilution in TBST at 25°C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: The DNA was denatured using 0.5 M NaOH and 1.5 M NaCl and equal amounts were loaded onto a Hybond N + nitrocellulose membrane (GE Biosciences) using the Bio-Dot apparatus (Bio-Rad). Membranes were washed once with denaturing buffer and wash buffer (3× SSC) ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then subjected to SDS-PAGE at a constant voltage of 100 V for 1 hour at room temperature and transferred to nitrocellulose membrane (BIO-RAD) at a constant voltage of 30 V at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Protein samples were resolved by SDS-PAGE on 10% or 15% Tris-Glycine buffered polyacrylamide gels and transferred to PVDF membranes using mini trans-blot module (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Transfer to a nitrocellulose membrane (Amersham Bioscience, Arlington Heights, IL, USA) was performed using a semi-dry apparatus (Trans-Blot SD, Bio-rad) and analysed by immunoblotting with enhanced chemiluminescence reagents (Amersham Bioscience ...
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bioRxiv - Cell Biology 2020Quote: Whole cell extracts were run on 10% SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus following the standard protocols (Bio-Rad). After incubation with 5% nonfat milk in TBST (10 mM Tris ...
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bioRxiv - Genetics 2021Quote: ... was used to dry transfer the material onto a PVDF membrane (Trans-Blot Turbo Mini 0.2 ul PVDF Transfer Packs, Bio-Rad, #1704156). After blocking with 5% skim milk in TBS-T ...
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bioRxiv - Genetics 2021Quote: ... separated using SDS-PAGE (4 to 12 % gradient gel at 150 V for 2.5-3 h) and transferred to a nitrocellulose membrane overnight (∼16 h) at 20 V using the Bio-Rad Transblot (Bio-Rad). Membranes were blocked in 5 % fat-free milk in 20 mmol/l Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... Equal amounts (30 μg) from each protein sample were separated by SDS-PAGE and transferred to PVDF membranes (PVDF; Biorad, USA). The membranes were blocked with 10% (W/V ...
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bioRxiv - Microbiology 2021Quote: ... Normalized volumes of samples from the different growth time points were applied on SDS-PAGE gels (10% acrylamide) and transferred to a nitrocellulose membrane using Mini Trans-Blot cells (Bio-Rad Laboratories Ltd. ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins on the gels were transferred onto PVDF membrane using BioRad Mini Trans-Blot system (BioRad, Mini Trans-Blot® Cell). The membranes were washed with pure water and blocked with 5% skimmed milk in 0.1% Tween-20/PBS (PBST ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were washed three times with TBST for 5 minutes and incubated with Goat Anti-Rabbit IgG (Bio-Rad, 170-6515) for 1 hour at RT ...
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bioRxiv - Developmental Biology 2020Quote: Equal amounts of protein were resolved on either a 15% or a 4-20% Tris-glycine gradient SDS-PAGE gel and transferred to a polyvinylidene difluoride Immobilon-FL membrane (PVDF; Bio-rad). Membranes were blocked for 30 min at room temperature with 5% BSA in TBST (50 mM Tris ...
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bioRxiv - Developmental Biology 2020Quote: ... and 50 µg of denatured proteins were run on a 12% acrylamide gel and transferred to a 0.2 µm nitrocellulose membrane (BioRad, Hercules, CA). We blocked membranes in 5% Milk in TBST (Tris Buffer Solution + 0.1% Tween ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins (20 μg) were separated using an Any kD gel (SDS-PAGE) and transferred to a nitrocellulose membrane (BioRad, Temse, Belgium). Aspecific binding sites were blocked for 30 min using TBS + 1% casein (BioRad ...
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to nitrocellulose membrane (Whatman Protran BA85, cat. No. 10401196) (25 V, 1 A, 3 0min using Trans Blot Turbo transfer system (Bio-Rad). Membrane was blocked with 5% milk and probed with anti-mAID (1:5,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... For western blotting, separated proteins were transferred onto a nitrocellulose membrane (Whatman, Dassel, Germany) using a Trans-Blot Turbo transfer system (Bio-Rad). The membrane was blocked and then incubated for 1 h with an His-tag-specific mouse mAb antibody (Proteintech ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were then separated on a 10% SDS-PAGE gel and transferred onto a nitrocellulose membrane (Bio-Rad, 162-0112). The membranes were incubated in a blocking solution (5% milk in TBS-T ...