Labshake search
Citations for New England Biolabs :
4601 - 4650 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2019Quote: The TDP-43 library was then prepared for deep sequencing by PCR amplification in two steps using Q5 High-Fidelity DNA Polymerase (NEB). In step 1 ...
-
bioRxiv - Genetics 2019Quote: ... Genomic regions of interest (spanning 2 kb upstream of the start codon ATG and the full coding sequence excluding stop codon) were amplified by PCR with Phusion DNA polymerase (NEB). Promoter::CDS amplicons were cloned via KpnI/BamHI restriction sites into a pGreen-IIS backbone (Basta resistance ...
-
bioRxiv - Plant Biology 2019Quote: ... All standard PCR reactions were performed with a 57°C annealing temperature using Taq polymerase with Standard Taq Buffer (New England Biolabs).
-
bioRxiv - Microbiology 2019Quote: ... a 328 bp internal fragment of phbC was PCR amplified from the K56-2 genome using primers 1196 and 1197 and Q5 polymerase (NEB). The plasmid ...
-
bioRxiv - Microbiology 2019Quote: ... a 322 bp internal fragment of fliF was PCR amplified from the K56-2 genome using primers 1156 and 1157 and Q5 polymerase (NEB). The fragment and pGPΩ-Tp were double digested with KpnI and EcoRI (NEB ...
-
bioRxiv - Microbiology 2019Quote: ... The full-length gene (with terminal NdeI and HindIII cut sites) was synthesized by overlap-extension PCR using Q5 polymerase with high GC buffer (NEB) and primers 979 and 987 (Supplemental Table 2) ...
-
bioRxiv - Plant Biology 2019Quote: ... RT-PCR reaction volumes were set up to 12.5 μL containing Luna Universal qPCR Master Mix (New England Biolabs Inc.), 2 μl of a 1:10 dilution of cDNA reaction ...
-
bioRxiv - Genomics 2019Quote: ... solution was diluted 1:20 and 5 μL used in a standard 50 μL PCR reaction using Q5 enzyme (NEB). PCR products were Sanger sequenced and analyzed using SnapGene to identify SNP corrected clones (7/192) ...
-
bioRxiv - Biochemistry 2019Quote: ... Quantification of the ratio of MS2-tagged rRNA to WT rRNA was done via semi-quantitative PCR with Q5 DNA polymerase (NEB). Primers MS2_quant_F/R bind outside the MS2 tagged region of 23S cDNA ...
-
bioRxiv - Molecular Biology 2020Quote: ... treated with a USER enzyme and then amplified via PCR using universal primers Next Multiplex Oligos for Illumina (New England Biolabs) and Kapa HiFi Polymerase (KAPA Biosystems) ...
-
bioRxiv - Biochemistry 2019Quote: ... fused to the 3’ end of the nucleotide sequence encoding full length Ypt7 was amplified by PCR from pET-19 Ypt7-tm (a kind gift from C Ungermann) with the Phusion high-fidelity DNA polymerase (NEB). The DNA fragment was cloned into BamHI and SalI digested pMBP-parallel1 vector (Sheffield et al. ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1ul of cDNA was used for the first PCR (20ul volume) performed with Taq DNA polymerase (New England BioLabs, M0273) and primers listed in Table S4 ...
-
bioRxiv - Synthetic Biology 2019Quote: ... All the PCR reactions were performed according to the Phusion® High-Fidelity DNA Polymerase (New England Biolabs, MA, USA) protocol using 0.05 Units of Phusion and 100 µM dNTPs in a total volume of 25 µl.
-
bioRxiv - Molecular Biology 2019Quote: ... a ~200 bp amplicon was generated from 1×106 template molecules by PCR in 22 cycles using Phusion polymerase (NEB), purified with the QIAquick gel extraction kit ...
-
bioRxiv - Molecular Biology 2019Quote: A 1 kb long DNA fragment with biotin molecules attached to the 5′ termini (dibiotin-DNA) was generated by PCR amplification using Q5 High-Fidelity Polymerase (NEB), with pAM075 as a template and the 5′-biotinylated primer pair oAM091/oAM092 ...
-
bioRxiv - Genomics 2020Quote: We used the prepared DNA of each clone and the wild type gene for amplification by PCR with Q5 polymerase (NEB) using primers which included the minION barcodes adapters ...
-
bioRxiv - Biochemistry 2020Quote: ... The region harboring the randomized hexamer and flanking constant tags was amplified from 1 µg ssDNA for 5 cycles using the Q5 Hot Start High-Fidelity PCR Master Mix (New England Biolabs) and the following primers ...
-
bioRxiv - Biochemistry 2020Quote: ... the nonstop GFP1-10 sequence was produced by PCR using primers #1 and 2 and Q5 High-Fidelity DNA Polymerase (NEB) with pETGFP 1-10 vector as a template (Cabantous et al. ...
-
bioRxiv - Genetics 2020Quote: ... Oligo pairs were mixed at an equimolar ratio in PCR tubes containing T4 Ligation buffer and T4 polynucleotide kinase (NEB) for 5’ phosphorylation of oligos ...
-
bioRxiv - Genomics 2021Quote: ... and on the other side a primer that targets a region introduced through the library preparation called “Read 1” (18 cycles using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs)) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and re-amplified the size-selected libraries using a NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs, Canada). Finally ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Riboprobe templates were synthesized from cDNA via standard PCR using opsin specific primers (Table S1) and Q5 High Fidelity DNA polymerase (New England Biolabs). Primers were designed to bind to the coding sequence of target opsins (SWS2B ...
-
bioRxiv - Cancer Biology 2021Quote: ... vector was generated by replacing the Ef1a promoter with a CMV promoter cassette in pLV-Ef1a-Cre-U6-sgRNA(MS2) using PCR and HiFi assembly (NEB). The pLV-CMV-CreERT2-P2A-[puroR/zeoR/hygR]-U6-sgRNA(MS2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... vector was generated by replacing the BleoR cassette with Cre recombinase from the pBS513 EF1alpha-Cre vector in the plenti sgRNA(MS2)_zeo backbone using PCR and HiFi assembly (NEB). One BsmBI site in the Cre cassette was destroyed through targeted mutagenesis ...
-
bioRxiv - Cell Biology 2021Quote: ... and DNA fragments were amplified by PCR with NEXTFlex primers (Primer 1 - 5’-AATGATACGGCGACCACCGAGATCTACAC; Primer 2 - 5’-CAAGCAGAAGACGGCATACGAGAT) and Phusion High-Fidelity DNA Polymerase (NEB) and further purified with AMPure XP beads to eliminate unligated primers and adapters ...
-
bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB ...
-
bioRxiv - Genomics 2019Quote: ... The first 60 μL PCR reaction (also run as three parallel 20 μL reactions) included 6 μL of 10X ThermoPol Taq buffer (NEB), 0.96 μL of 5 μM forward primer (0.08 μM final) ...
-
bioRxiv - Cell Biology 2021Quote: ... which were then digested by SacI/MscI and ligated with SacI/MscI-digested second PCR fragment by T4 DNA ligase (M0202S; NEB). Linear cc2 constructs were obtained by SacI/KpnI digestion of the resulting plasmids and transformed into desired strain for cc2 insertion.
-
bioRxiv - Plant Biology 2020Quote: ... the genomic regions containing pre-miR775a and the GALT9 coding region were PCR amplified using the Pfusion DNA polymerase (New England Biolabs) and primers listed in Supplemental Table 2 ...
-
bioRxiv - Microbiology 2021Quote: PCR reactions to amplify the trailer sequences from the circularized cDNA using Q5 High-Fidelity DNA Polymerase (New England Biolabs) using 60 ng of circularized template were then set up with the following parameters ...
-
bioRxiv - Microbiology 2021Quote: ... The resulting PCR fragment and the NdeI-NheI-cut pMT571 were assembled together using a 2x Gibson master mix (NEB). Gibson assembly was possible owing to a 23 bp sequence shared between the two DNA fragments ...
-
bioRxiv - Molecular Biology 2020Quote: ... Subcloned cells were screened for correct targeting by PCR amplification and restriction enzyme digestion (MCM10 exon 3, Hpy199III (NEB R0622); CDC45 exon 3 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... parts were domesticated removing BpiI and BsaI sites by PCR-based mutagenesis and cloned into corresponding vectors by GG cloning using either BsaI (NEB) or BpiI (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... The Purified PCR product was treated with Cre recombinase as described in the instructions (M0298, New England Biolabs, Ipswich, MA). The CaMK1 floxed PCR product and control plasmid (pLOX ...
-
bioRxiv - Cancer Biology 2020Quote: ... PCR was carried out with primers targeting UPF1 exons 9 and 12 using Q5® High-Fidelity DNA Polymerase (NEB) (Table S5) ...
-
bioRxiv - Molecular Biology 2021Quote: ... genomic DNA isolated from Xanthomonas albilineans CFBP7063 was PCR amplified using Q5 Hot Start High-Fidelity 2X Master Mix (NEB) and cloned into pET28a using Gibson Assembly ...
-
bioRxiv - Microbiology 2020Quote: ... ∼1.2 kb upstream and downstream of the tcdR gene were PCR amplified with Phusion High-Fidelity DNA polymerase (NEB M0530S). All primers used for construction of the mutagenesis vector and for PCR screening of transconjugants can be found in Supplemental Table 1 ...
-
bioRxiv - Biochemistry 2020Quote: ... Cas9 variant plasmids were constructed via ATW PCR and a KLD reaction and/or via DNA assembly using New England Biolabs (NEB) HiFi DNA Assembly (NEB E5520S) ...
-
bioRxiv - Biochemistry 2020Quote: DNA fragments containing sgRNA sequences were amplified from isolated genomic DNA by two rounds of PCR using NEBNext Ultra II Q5 master mix (NEB). For the first round of PCR ...
-
bioRxiv - Biochemistry 2020Quote: DNA templates for in vitro transcription were amplified by PCR using custom DNA primers (IDT) and Phusion Hot Start polymerase (New England BioLabs). 2.5 mL transcription reactions were assembled using a 1000 μL PCR reaction ...
-
bioRxiv - Biochemistry 2020Quote: ... Modification of the coding sequence of the multibasic S1/S2 cleavage site PRRAR to PGSAS or to a single R was carried out by PCR mutagenesis using Q5 polymerase (New England Biolabs). Introduction of cysteine crosslinks and modification of residues 986 and 987 were carried out using Q5 polymerase PCR with primers containing desired substitutions ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 μl of cDNA was amplified by PCR with primers that amplify about 300-600 bp surrounding the sites of interest (outside the length of the antisense domain) using OneTaq PCR Mix (NEB). The numbers of cycles were tested to ensure that they fell within the linear phase of amplification ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 µL linearized expression plasmid was assembled with 3 µL each of PCR amplified product using 4 µL of NEBuilder HiFi DNA Assembly MasterMix (#E2621L, New England Biolabs) in a 96-well plate format ...
-
bioRxiv - Bioengineering 2021Quote: ... Synthesized library inserts 1-16 were mixed in equimolar ratios and extended to the XbaI recognition site on the pET backbone using Q5 PCR (NEB) with the library insert as a template and XbaI extension and common reverse as primers (Supplementary Table 5) ...
-
bioRxiv - Microbiology 2021Quote: ... All primers (Supplementary Table 2) were purchased from Eurofins Genomics and all PCR reactions were performed using Q5 High-Fidelity (New England Biolabs). HIV-1NL4-3 (pHIV-1WT ...
-
bioRxiv - Biochemistry 2021Quote: ... 1000 base pairs upstream and downstream from the clpP2 gene were amplified by PCR using the A–B and C–D primer pairs from table 3 (Phusion polymerase, GC buffer, New England Biolabs). The PCR products were purified with E.Z.N.A ...
-
bioRxiv - Biochemistry 2020Quote: ... Human wild-type TDP-43 was amplified in two separate PCR reactions excluding the NLS and reassembled using Gibson cloning (NEB) into a Doxycycline-inducible expression vector containing an N-terminal mClover3 tag ...
-
bioRxiv - Biochemistry 2020Quote: ... The entire mDHFR ORF was PCR amplified from a mDHFR plasmid with a C-terminal AviTag fused to the mDHFR fragment using Gibson Assembly (NEB). The backbone contained a T7 promoter ...
-
bioRxiv - Biophysics 2021Quote: ... Hybridization reactions were split into two and libraries re-amplified using Post LM-PCR oligos (Nimblegen) and Q5 High-Fidelity DNA polymerase (NEB) directly from the beads ...
-
bioRxiv - Neuroscience 2021Quote: ... genomic PCR products containing the target sites of selected gRNAs were incubated with SpCas9 protein (New England Biolabs, Ipswich, MA) following the manufacturer’s protocol and analyzed on 2% agarose gel stained with ethidium bromide ...