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Citations for New England Biolabs :
4551 - 4600 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products of ITS and EST104 were digested with DdeI and RsaI (New England Biolabs Japan, Inc., Tokyo, Japan), respectively ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and GFPmut2) and segregating promoter variants were PCR amplified using Phusion High-Fidelity DNA polymerase with HF buffer (New England Biolabs). For promoter PCR amplification ...
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bioRxiv - Developmental Biology 2020Quote: ... and a 481-bp pph-5 3’UTR and assembled the PCR products with amplified pUC57 backbone via HiFi assembly (New England Biolabs) to generate the vector pph-5p::pph-5(cDNA)::pph-5 3’UTR (TU#2257) ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... The human MRLC2 gene from pCS2+-MRLC2-GFP 47 was used for creation of hsp70:MRLC-mKate2 construct which was then used as template for construction of MRLC2-mKate2 middle entry clone (pME) by PCR using Phusion polymerase (New England Biolabs) and primers with ATT recombination site (shown in lower case) ...
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bioRxiv - Biophysics 2022Quote: ... Oct4 was cloned into the pOPIN expression vector using the SLIC method and Phusion Flash High-Fidelity PCR Master Mix (Finnzymes/New England Biolabs). SLIC reactions were then transformed into One Shot™ OmniMAC™ 2 T1® Chemically Competent E ...
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bioRxiv - Neuroscience 2021Quote: ... and PCR amplified for 13 cycles with Illumina Nextera adapter primers using the NEBNext High Fidelity 2X Master Mix (NEB) with the following PCR program ...
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bioRxiv - Molecular Biology 2021Quote: ... CMTr1 was amplified from this cDNA with primers pUAST CG6379HA F2 (CGAACCTTCGGACGATGAGAACTCGGAGCCCACGCCCAAGAAG) and pUAST CG6379 F3 (GCAGAATTCGAGATCTAAAGAGCCTGCTAAAGCAAAAAAGAAGTCACCATGGA CGAACCTTCGGACGATGAGAACTCG) with return primer R5 Spe in a nested PCR with Q5 polymerase (NEB) and cloned with EcoRI and SpeI into a modified pUAST vector containing an attB site for phiC31 mediated integration ...
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bioRxiv - Molecular Biology 2021Quote: ... Mpe1P215G and Mpe1W257A/Y260A variants were similarly generated with PCR products carrying the respective mutations and Gibson assembly into a BamHI (NEB) digested pACEBAC_TEV_SII vector ...
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bioRxiv - Molecular Biology 2020Quote: Nested PCR was performed with appropriate primers (Supplementary Table S3) using OneTaq Hot Start DNA Polymerase (New England Biolabs, M0481) and OneTaq Standard Reaction Buffer (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... Gluc200 and Gluc200A44 templates were generated using PCR amplification of GLuc of the first 200 nt at the 5’end of pCMV-GLuc 2 Control Plasmid (NEB: https://www.neb.com/tools-and-resources/interactive-tools/dna-sequences-and-maps-tool) ...
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bioRxiv - Molecular Biology 2021Quote: ... resistance as well as a replication origin p15A (oriE) was PCR amplified by using Q5 High-Fidelity DNA Polymerase (New England Biolabs) with primers pACshtl-for (5’-TTCACGCGTAGCACCAGGCG ...
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bioRxiv - Molecular Biology 2021Quote: A CD22 cDNA fragment encoding the first two Ig-like domains fused to an EK-hIgG-Fc fragment was amplified by PCR and cloned into the mammalian expression vector pACP-tag(m)-2 (New England Biolabs).
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bioRxiv - Cell Biology 2022Quote: The DNA sequence coding for full length of p21 and Cyclin B1 was amplified from mouse cDNA by PCR using Phusion high-fidelity DNA Polymerase (New England BioLabs) and cloned in frame into Ch plasmid with AsiSI and NotI restriction endonucleases (New England BioLabs) ...
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bioRxiv - Genomics 2019Quote: The sequence of bovine APOB ERV was generated by PCR amplifying the full length ERV with LongAmp Taq DNA Polymerase (New England Biolabs) from a Holstein suffering from cholesterol deficiency ...
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Reprogramming enriches for somatic cell clones with small scale mutations in cancer-associated genesbioRxiv - Genomics 2020Quote: ... PCR products of 1400-1600bp were generated by running 20 PCR cycles with a Q5 Hot Start High Fidelity DNA Polymerase (NEB GmbH ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl of the cDNA was used as template for the RT-PCR reaction with OneTaq DNA Polymerase (New England Biolabs). PCRs were run at the following conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... were used in combination with mutation primers (Table 1) to generate overlapped PCR fragments (Phusion High-Fidelity DNA Polymerase, NEB), with disrupted RNA structure at each selected PAR-CL sites ...
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bioRxiv - Cell Biology 2019Quote: ... the eGFP-FLAG-HA-MLKS2 sequence was PCR amplified with att flanking primers (Table S2) using high fidelity Q5 polymerase (NEB) and cloned into pDONR221 vector by BP cloning (Cat ...
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bioRxiv - Microbiology 2019Quote: ... The PCR product encoding for sadBA and the PCR product from vector p4392 were assembled by Gibson assembly according to manufactureŕs protocol (NEB Monarch). For overexpression of the sii operon ...
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bioRxiv - Genetics 2020Quote: ... Each of them was made by assembling a synthetic gBlock DNA fragment with a PCR-amplified Kanamycin resistant backbone using NEBuilder DNA Assembly (New England Biolabs). The region to be PCR-amplified in each vector contains a gRNA scaffold followed by either a tRNA or the U6:3 promoter ...
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bioRxiv - Developmental Biology 2019Quote: ... and Tol2-elavl3:mCherry-CAAX was assembled from the digested plasmid and PCR products using Gibson Assembly Master Mix (NEB). The insert was sequenced to confirm ligation fidelity.
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bioRxiv - Developmental Biology 2019Quote: ... and Tol2-elavl3:GAVPO was assembled from the resulting vector and GAVPO PCR product using Gibson Assembly Master Mix (NEB). The entire plasmid was sequenced to confirm ligation fidelity.
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bioRxiv - Microbiology 2019Quote: ... All restriction enzymes were obtained from New England Biolabs and all PCR was performed with primers from Integrated DNA Technologies and Q5 polymerase (New England Biolabs). All cloning steps were performed in π-carrying hosts (either DH5αpir (41 ...
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bioRxiv - Molecular Biology 2019Quote: ... Oligonucleotides Gb-155Sph and Gb-AD-155 (Table S2) were used to generate a PCR fragment encoding HSH155 for Gibson assembly (NEB) into the pACT plasmid ...
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bioRxiv - Microbiology 2019Quote: ... the vector backbone and gene fragments were amplified by PCR using Q5 DNA polymerase (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... The gene fragment covering the predicted middle domain (residues 807–1097) was amplified by PCR using Phusion High-Fidelity DNA Polymerase (New England Biolabs), using pcDNA3-Xpress-G5 as template (Francisco-Velilla et al. ...
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bioRxiv - Cell Biology 2019Quote: ... The 3xHA epitope was PCR-amplified from a pFA6a-3xHA-his3MX6 (Longtine et al. 1998) plasmid using Q5 DNA polymerase (New England BioLabs) and assembled into pFA6a-GFP-hisMX6 (Longtine et al ...
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bioRxiv - Developmental Biology 2019Quote: ... Repair templates were generated by inserting 500 bp homology arm PCR products into destination vectors containing egfp and a self-excising selection cassette using Gibson assembly (New England Biolabs) or SapTrap assembly (Dickinson et al ...
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bioRxiv - Biochemistry 2019Quote: ... and a long primer (C1-EGFP-NES long forward: ttaatgtacaagggtgcatcctctgcaagtggcaacagcaatgaattagccttgaaattagcaggtcttgatatcaacaagtaagcggccgcttaa) covering the whole peptide using Polymerase chain reaction (PCR; Phusion Polymerase, New England Biolabs (NEB)) ...
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Calibrated feedback illumination for precise conventional fluorescence and PALM imaging applicationsbioRxiv - Biophysics 2019Quote: ... Wild-type ADE2 was amplified from genomic DNA, RB201 (W303 MATa, trp1, leu2, ura3, his3, can1R, ADE2) with Phusion PCR (NEB) using the forward primer (ATGGATTCTAGAACAGTTGGTATATTGGGAGGGGGACAA ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR amplification of the target locus (forward primer: 5’-GGTTCTCAGTGCACGCATTT-3’; reverse primer: 5’-ACAACGATTTTCCTGGCATCT-3’) with Q5 polymerase (NEB), and Sanger sequencing of PCR products by the Keck Biotechnology Resource Laboratory at Yale ...
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bioRxiv - Microbiology 2019Quote: ... 1 kb fragments of the 5’ and 3’ flanks of the gene of interest (goi) ORF were generated by PCR using Phusion High Fidelity DNA polymerase (New England Biolabs) or Q5 High-Fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 1 µL was used as the template in a PCR amplification containing 0.5 µL of Q5 High-Fidelity DNA Polymerase (NEB, M0491S), 1x Q5 polymerase reaction buffer (NEB ...
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bioRxiv - Synthetic Biology 2019Quote: ... Unique forward primers flanked with a SpeI restriction site and encoding the new target sequence and a common reverse primer flanked with ApaI was used to PCR amplify (Phusion, New England Biolabs) new DNA inserts ...
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bioRxiv - Bioengineering 2019Quote: ... the 720 bp Cerulean cassette between the AgeI/HpaI sites of pPPIA-Cer-Fib was replaced with a 560 bp SNAP tag fragment PCR amplified from plasmid pSNAPf (New England Biolabs) using primer pair Snap-XmaI-For/Snap-HpaI-Fib-Rev and double digested with XmaI/HpaI ...
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bioRxiv - Bioengineering 2019Quote: ... the GFP cassette between KpnI/HpaI restriction sites of plasmid GFP-Fibrillarin was replaced with a 730 bp Cerulean cassette PCR amplified from plasmid pCerulean-N1 (New England Biolabs) using primer pair ForCerFib/RevCerFib ...
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bioRxiv - Developmental Biology 2020Quote: DNA was extracted from embryonic membranes or tail tissue using the HotShot method for 15 minutes (Truett et al., 2000) and 2 µl DNA was used for PCR with Phusion polymerase (New England Biolabs). For Geminin and Cre PCRs ...
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bioRxiv - Genomics 2019Quote: ... Libraries were produced by PCR amplification (12-14 cycles) of tagmented DNA using the NEB Next High-Fidelity 2x PCR Master Mix (New England Biolabs). Library quality was assessed in an Agilent Bioanalyzer 2100 ...
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bioRxiv - Microbiology 2020Quote: ... in a new PCR tube 2.5 µL cDNA was combined with 12.5 µL Q5 High-Fidelity 2X Master Mix (NEB, Ipswich, USA), 8.8 µL nuclease free water (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: The PCR reaction was motivated in 30 μL reaction systems after mixing 15μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers labelled with specific barcodes ...
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bioRxiv - Developmental Biology 2019Quote: ... and the bar-1 locus was genotyped for successful co-conversion by PCR amplification with OneTaq polymerase (New England Biolabs) using primers that annealed to the genomic DNA surrounding the insertion point ...
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bioRxiv - Biochemistry 2019Quote: The required coding region was amplified from the H6-TtBac construct by PCR and was cloned into the plasmid pHis17 using Gibson Assembly (New England Biolabs), resulting in a C-termin tag ...
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bioRxiv - Biochemistry 2019Quote: ... Genes encoding for each nanobody were amplified using colony PCR and cloned into the pHEN6c expression vector (VIB) using Gibson Assembly (New England Biolabs). The resulting constructs using pHEN6c encoded for PelB(leader)-nanobody-SSHHHHHH proteins.
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bioRxiv - Biochemistry 2019Quote: The required coding region was amplified from the H6-TtBac construct by PCR and was cloned into the plasmid pHis17 using Gibson Assembly (New England Biolabs), The plasmid was used to transform C41(DE3 ...
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bioRxiv - Plant Biology 2019Quote: The promoter region and full-length CKX2I or coding DNA sequence (CDS) were amplified by PCR (Table S2) from genomic DNA or cDNA using Q5 High-Fidelity DNA Polymerase (NEB) and cloned either alone or under of the 35S promoter together with GFP and mScarlet-i into pPLV03 or pGEX5x3 using Gibson Assembly (NEB) ...
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bioRxiv - Cancer Biology 2019Quote: ... 200 ng of purified PCR product were denatured and re-annealed in NE Buffer 2 (New England BioLabs, Cat. # B7002S). T7 endonuclease 1 (New England BioLabs ...
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bioRxiv - Molecular Biology 2019Quote: ... Final libraries for each ChIP were produced using 150-200 ng of purified cDNA in a PCR reaction (High-Fidelity 2x master mix, New England BioLabs) for 8 cycles with 0.2 μM primers that carried a second 8bp barcode sequence ...
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bioRxiv - Microbiology 2019Quote: ... 500 bp upstream and 500 bp downstream of the gene to be deleted were amplified by overlapping PCR with Q5 high fidelity DNA polymerase (NEB) using primers listed in Supplementary Table 1 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the editing domain-coding segment of leuS gene was PCR amplified by using Phusion® High-Fidelity DNA Polymerase (NEB), primers 1 and 2 ...
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bioRxiv - Synthetic Biology 2019Quote: ... the original pSJ051 plasmid was mutated at the RBS upstream of triA (changed from AGGAGA to AGAAGA) by inverse PCR (Liu and Naismith, 2008) using Q5 DNA polymerase (NEB) using primers D101108989 ...