Labshake search
Citations for New England Biolabs :
4351 - 4400 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: 75ng of genomic DNA were used for PCR amplification in two 50µl reactions with 25µl 2xQ5 mix (NEB M0492), 2.5µM of each forward (FOS ...
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bioRxiv - Microbiology 2022Quote: ... and the three PCR products were joined using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs) according to manufacturer’s recommendations and transformed into NEB5α cells (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... a product flanking the sgRNA target sites was PCR-amplified and subjected to a T7 Endonuclease I assay (NEB) to detect indels ...
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bioRxiv - Microbiology 2022Quote: ... pCHYC-1 and a 500 bp ‘hfaE C-terminal PCR product were digested with HindIII-HF and KpnI (NEB) and ligated with T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... NbALFA-LgBiT and LgBiT-NbALFA were amplified by PCR and cloned in pcDNA3.1+ using NEB HiFi DNA Assembly (New England Biolabs).
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bioRxiv - Molecular Biology 2023Quote: ... βArrestin2-ALFA-GFP2 were amplified by PCR and cloned in pcDNA3.1+ using NEB HiFi DNA Assembly (New England Biolabs). Gβ3 and Gγ2-GFP2 were generously gifted by Dr ...
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bioRxiv - Molecular Biology 2023Quote: ... The sir2 ORF was amplified by PCR using Q5 High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA, USA) and cloned into p123 within the NcoI and NotI restriction sites of p123 ...
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bioRxiv - Microbiology 2023Quote: ... Phage genes were amplified from Butters genomic DNA by PCR using Q5 high-fidelity DNA polymerase (New England Biolabs). PCR products were digested with XbaI overnight (O/N) ...
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bioRxiv - Neuroscience 2023Quote: Tail tip genomic DNA was PCR amplified with ATRX_RC gen F and ATRX_RC gen R primers and digested with FspI (NEB R0135S). FspI cuts the wild-type allele (product sizes ...
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bioRxiv - Systems Biology 2022Quote: ... PCRs for screens with all other libraries were performed using the NEBNext Ultra II Q5 Master Mix (NEB #M0544L) with 21 to 24 cycles ...
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bioRxiv - Microbiology 2022Quote: ... and the PCR product was linked with pBOMBL::L2 linearized by EagI and KpnI using HiFi assembly system (NEB). The HiFi products were transformed into NEB10β competent cells (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... a DNA template was generated by PCR and then used for RNA synthesis (HiScribe T7 RNA polymerase, NEB #E2040S). DNA template was removed from the reaction with Turbo DNAse (Invitrogen #AM2238) ...
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bioRxiv - Microbiology 2022Quote: ... PCR was performed to amplify the PA3626 gene using the manufacturer’s protocols for Phusion Taq polymerase (New England Biolabs) and forward (5’-GCGCGGATCCATGAGCGTTCTCGGCGAA ...
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bioRxiv - Neuroscience 2022Quote: ... The last two PCR rounds were performed using NEBNext® Ultra™ II Q5® Master Mix (NEB #M0544). Samples were purified using AMPure XP beads (Beckman Coulter #A63880) ...
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bioRxiv - Molecular Biology 2022Quote: ... The total isolated gDNA was processed in batches of 5 µg per PCR reaction with Q5 polymerase (NEB M0491L). One PCR reaction contained 10 µl 5x reaction buffer ...
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bioRxiv - Microbiology 2022Quote: ... Deletion mutants of 2xStrep-ORF45 were generated using site-directed mutagenesis PCR with Q5 DNA Polymerase (New England Biolabs) with primers listed in Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... Conventional PCR assays were carried out with Q5 Hot Start High-Fidelity 2× Master Mix (New England Biolabs, Australia). For some samples ...
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bioRxiv - Synthetic Biology 2022Quote: ... long overlapping primers tRNA-fMet-C1G_temp F and RNA-fMet-C1G-A_temp R (Extended Data Table 1) were PCR amplified using Q5 DNA polymerase (NEB). Products were gel purified and amplified using short primers tRNA-fMet-C1G_amp F and tRNA-fMet-C1G-A_amp R (Extended Data Table 1) ...
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bioRxiv - Molecular Biology 2022Quote: Sequencing libraries were prepared from 52.5 μg gDNA using two-step PCR strategy with NEBNext Ultra II Q5 Polymerase (NEB). First the gRNA regions were enriched from the genome ...
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bioRxiv - Synthetic Biology 2024Quote: ... The inserts were generated using PCR or gBlock synthesis (IDT) and were ligated either by T4 ligase (NEB #M0202M) or In-Fusion (Takara #102518 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μL i7 unique index primer (10 μM) and 25 μL NEBNext High-Fidelity 2X PCR Master Mix (NEB) were added to 21 μl of purified CUT&TAG DNA ...
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bioRxiv - Systems Biology 2024Quote: ... the cDNA was diluted to 0.05 µg/ml and PCR-amplified using Phusion DNA polymerase (New England Biolabs M0530) with 0.5 mM dNTPs ...
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bioRxiv - Microbiology 2024Quote: ... the sequence encoding amino acid residues 1 to 35 of the N-terminal end of BVG96_RS17270 (89) was PCR amplified using Q5 polymerase (NEB) and cloned via NEBuilder HiFi DNA Assembly (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and menA were amplified by PCR from the IR715 genome using Q5 High Fidelity Master Mix (New England Biolabs). PCR products were purified using the QIAEX II Gel Extraction Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using unique dual index primer pairs (NEBNext Multiplex Oligos for Illumina from New England BioLabs, # E6440S) according the following parameters ...
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bioRxiv - Microbiology 2024Quote: ... Colony PCR was performed on Salmonella-positive colonies on blood agar using Taq DNA polymerase (New England Biolabs®) with an annealing temperature of 55°C and an extension time of 2.5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... The purified DNA was amplified as described before with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs). The PCR amplified product was purified and size selected twice with AMPure beads (Beckman).
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bioRxiv - Microbiology 2024Quote: ... PCR was performed with medium range primers 24 using Q5 High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Input genomic DNA was first amplified in a 10μL reaction for 30 cycles using NEBNext High-Fidelity 2×PCR Master Mix (NEB). Amplicons were purified using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... and genome-integrated sgRNA sequences were amplified by PCR using Q5 Mastermix (NEB Next UltraII, New Endland Biolabs M5044S). i5 and i7 multiplexing barcodes were introduced in a second round of PCR and final gel purified products were sequenced on Illumina NextSeq500 systems to determine sgRNA representation in T0 and T18 time points of each sample ...
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bioRxiv - Genomics 2024Quote: ... to the PCR-amplified pDR111 (using P3 and P4) using the HiFi DNA assembly protocol (New England Biolabs, USA). This resulted in the cloning of the mutL* polC* synthetic operon under the control of Phs into a B ...
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bioRxiv - Microbiology 2024Quote: ... the DNA in 11.5 µL in a PCR tube was end-repaired by addition of 1.75 µL NEB Ultra II End Repair Buffer (NEB) and 0.75 µL Enzyme Mix followed by thorough mixing via pipetting ...
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bioRxiv - Microbiology 2024Quote: Test PCRs on cDNA were performed in a 50 µL scale using 1.0 µL precipitated cDNA in the presence of 1 x High-Fidelity PCR MasterMix (NEB), 2 µM forward primer with native barcode and 2 µM reverse primer with native barcode ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR reactions were performed using Phusion® high-fidelity DNA polymerase according to the manufacturer’s protocol (New Englang BioLabs). Amplified flanking recombination regions were merged with the MX cassette through Circular Polymerase Exchange Cloning into the pUC19 plasmid [67] ...
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bioRxiv - Genetics 2024Quote: ... The PCR products were cloned into the pSpl3-Flu2-TK-del vector using Gibson Assembly Master Mix (NEB, USA).
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bioRxiv - Microbiology 2024Quote: Final PCRs on cDNAs were performed in a 100 µL scale using 2.0 µL precipitated cDNA in the presence of 1 x High-Fidelity PCR MasterMix (NEB), 2 µM forward primer with native barcode and 2 µM reverse primer with native barcode (e.g BC1 fwd and rev ...
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bioRxiv - Biochemistry 2024Quote: Sequencing libraries for each sorted bin were generated by two-step PCR using a Phusion® DNA Polymerase (NEB) kit ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR reaction was mixed by combining 25 μL of Q5 High-Fidelity DNA Polymerase (New England Biolabs #M0494S), 2 μL of the oligo pool solution ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were amplified using inner primers ACL-P1060 and ACL-P1061 in a 40μl PCR reaction using Q5 Hot Start 2x Master Mix (New England Biolabs) for 20-25 cycles at an annealing temperature of 64°C ...
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bioRxiv - Cancer Biology 2024Quote: ... the pooled oligo library was amplified using NEB Next High-Fidelity PCR Master Mix (New England Biolabs, cat#M0541S). The PCR product was digested with Esp3I (BsmBI ...
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bioRxiv - Cancer Biology 2024Quote: ... The adaptor-ligated DNA fragments were enriched by PCR using the Illumina Barcode primers and Phusion DNA polymerase (NEB). PCR products were size-selected using 3% NuSieve agarose gels (Lonza ...
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bioRxiv - Synthetic Biology 2023Quote: ... Unmethylated linear DNA was generated by PCR using NEB’s Q5 HotStart High-Fidelity 2x Master Mix (NEB CN# M0494S) from plasmid extracted from E ...
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bioRxiv - Neuroscience 2023Quote: SadCas9-2xKRAB-p2a segment was amplified by PCR from backbone using high-fidelity polymerase (Phusion, NEB, Cat. No. M0530S); and tdTomato was PCR amplified from a proprietary expression vector using high-fidelity polymerase ...
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bioRxiv - Cell Biology 2023Quote: ... The IMS-BirA construct and PCR products from ER-and PO-BirA products were cut with HindIII (NEB, R3104) and BamHI (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fragments for Gibson assembly were generated by PCR using Q5 Hot Start High-Fidelity polymerase (New England Biolabs, USA). PCR products were analyzed by agarose gel electrophoresis and bands of the expected size were excised ...
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bioRxiv - Microbiology 2023Quote: ... PCR analysis of total DNA extracted from oysters (N=60) used the high fidelity Q5 polymerase (New England Biolabs) in a total volume of 50 μL under the following conditions ...
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bioRxiv - Microbiology 2023Quote: ... The PCR fragments were inserted into the plasmid pG+host9 digested with PstI (New England Biolabs, Ipswich, Massachusetts, USA) by Gibson Assembly (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were separated by electrophoresis on 1.5% agarose gels stained with Green Gene Safe DNA Dye (Southern BioLabs). PCR products were purified using Zymo’s DNA Clean and Concentrator kit and sequenced with their respective primer sets to confirm correct insertion of the fluorescent tag.
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bioRxiv - Microbiology 2023Quote: ... Gibson assembly was performed using the generated PCR products and linearized pACYC184 according to the manufacturer’s protocol with HiFi DNA Assembly Master Mix (New England Biolabs). The pdxAoperon amplicon and pACYC184 was ligated after digestion using T4 DNA ligase according to the manufacturer’s protocol (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... Deletion variants of ATI2 were amplified from ATI2 (AT4G00355.1) CDS by overlap extension PCR using Phusion High Fidelity DNA polymerase (NEB) and subcloned into pCR™4/TOPO™ vectors (Invitrogen) ...