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Citations for New England Biolabs :
3701 - 3750 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Linearized pExTra01 plasmid was prepared for isothermal assembly reactions via PCR (NEB Q5 HotStart 2X Master Mix) of pExTra01 using divergent primers pExTra_F and pExTra_R ...
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bioRxiv - Microbiology 2023Quote: ... The whole genomic sequence of TgAPT1 (www.toxodb.org accession number TGME49_261070) was amplified by PCR with the Q5 High Fidelity polymerase (New England Biolabs) from Pru genomic DNA ...
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bioRxiv - Genetics 2023Quote: ... The PCR products containing the geneticin128-795-TtrpCand LB border were digested using AgeI (New England Biolabs) followed by a ligation reaction ...
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bioRxiv - Genomics 2023Quote: ... The purified DNA was amplified using a reaction mixture containing 2X NEB PCR Master Mix (NEB M0541L), a universal forward primer i5 ...
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bioRxiv - Cell Biology 2023Quote: ... The integrity of MS2×128-containing lines was further confirmed by PCR using Q5 (New England Biolabs) and Terra (Takara ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were performed using the high-fidelity polymerase Q5 (New England Biolabs - NEB M0492 or M0493) and purified using a DNA clean and concentrate kit (Zymo Research #D4014) ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR products were purified using Phusion High-Fidelity DNA polymerase (New England Biolabs, Frankfurt a.M., Germany) and subse-quently ligated into the vector system pCR-Blunt II-TOPO via the Zero Blunt™ TOPO™ PCR cloning kit (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: PCR reactions were performed using NEB EpiMark Hot Start Taq DNA Polymerase (New England Biolabs, catalog #M0490L). The following program was used for all bisulfite-converted genomic DNA PCRs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then amplified by performing 20 cycles of PCR using Q5® High-Fidelity 2X Master Mix (NEB) (final concentration between 30-100 ng.µl-1) ...
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bioRxiv - Cancer Biology 2023Quote: ... lentiviral fragments encompassing the pre-miRNA sequence were amplified by PCR using the High-Fidelity MasterMix (NEB) and 400 ng genomic DNA ...
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bioRxiv - Microbiology 2023Quote: ... was amplified in PCR using Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs) using primers flanked with SfiI restriction enzyme recognition sequence (underlined ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using OneTaq Quick-Load 2X Master Mix with Standard Buffer (New England Biolabs, USA). DNA assembly was done by using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... then was PCR amplified using Q5 Hot Start high fidelity DNA polymerase (New England Biolabs, Catalog #M0493S), and subcloned into a bacterial expression vector (Addgene plasmid #29656 ...
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bioRxiv - Cancer Biology 2022Quote: ... All PCR reactions were carried out using the Q5® High-Fidelity DNA Polymerase (New England Biolabs). pCL-ECO was used to generate retroviral virions ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: PCRs were performed with the Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs M0494S) and primers outlined in Table S1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The TERT promoter was then amplified with PCR using corresponding primers with the Q5 DNA polymerase (NEB) (Supplementary Table S1) ...
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bioRxiv - Biophysics 2023Quote: ... azide or 2xbiotin at the other end) were amplified by PCR from λ DNA template (NEB, N3011S). The primers were added to total 8 ml PCR mixture ...
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bioRxiv - Cell Biology 2022Quote: ... PCR reactions were carried out using Q5 High Fidelity Hot Start DNA Polymerase (New England Biolabs (NEB)) ...
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bioRxiv - Cell Biology 2022Quote: ... PCR reactions were carried out using Q5 High Fidelity Hot Start DNA Polymerase (New England Biolabs (NEB)) ...
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bioRxiv - Genetics 2023Quote: ... PCR reactions (15 μL) contained 7.5 μL of 2X OneTaq 2X Master Mix with Standard Buffer (NEB), 5.9 μL H2O ...
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bioRxiv - Genetics 2023Quote: Next-generation sequencing (NGS) amplicons were prepared by PCR amplification using Q5 High-Fidelity DNA Polymerase (NEB). 250 ng of template DNA was amplified in 15 cycles during the PCR1 step ...
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bioRxiv - Evolutionary Biology 2023Quote: ... All PCR reactions were performed using Q5 High-Fidelity master mix (New England Biolabs, Ipswich, Massachusetts, USA). For the first stage PCR ...
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bioRxiv - Genetics 2022Quote: ... genomic target sites were PCR-amplified (Q5® Hot Start High-Fidelity DNA Polymerase, New England Biolabs) using primers encoding Illumina adapter sequences (Supplementary Table 4) ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... Genes of interest were amplified by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs #M0491L). All genes were inserted into either pLV-Ef1a-V5-LIC-IRES-PURO (Addgene #120247 ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR products were digested with DpnI and assembled with Gibson assembly master mix (New England Biolabs) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... 10 µl of purified PCR product was used in a 20 µl HiFi assembly reaction (NEB, E2621S). HiFi assembly was performed at 50°C for 1 hour.
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bioRxiv - Immunology 2022Quote: ... A first round of PCR was performed using 1× Standard-Taq magnesium free reaction buffer pack (NEB) with 2 mM MgCl2 (NEB) ...
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bioRxiv - Genetics 2023Quote: ... and various PCR-amplified inserts were then added via Gibson Assembly (New England BioLabs Cat. No. E2611.) To generate pAw-Gal4DBD-3×127D01 ...
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bioRxiv - Genetics 2023Quote: ... and amplified for 5 cycles using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs; M0541L). After evaluation by real-time PCR ...
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bioRxiv - Biophysics 2023Quote: ... All DNA fragments were generated by PCR using Q5 Hot Start High-Fidelity DNA polymerase (NEB # M0494S). The primers for each fragment had gene-specific 3’- sequences and 15–20 additional bases at the 5’ end that overlap with the ends of the adjacent fragment ...
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bioRxiv - Microbiology 2023Quote: ... coli plasmid DNA were amplified by PCR with Phusion High-Fidelity DNA Polymerase (New England Biolabs, NEB) using primers as indicated in Table S1 ...
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bioRxiv - Molecular Biology 2023Quote: ... hassiacum strain DSM 44199 as a template for colony-PCR with Q5 High-Fidelity DNA polymerase (NEB) to generate a fragment cloned into initial vector pINIT (Cm25) ...
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bioRxiv - Molecular Biology 2023Quote: ... Primers were used in a polymerase chain reaction (PCR) with 2X Phusion Master Mix (New England Biolabs) and the appropriate parent plasmid ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were performed using the high-fidelity polymerase Q5 (New England Biolabs - NEB M0492 or M0493) and purified using a DNA clean and concentrate kit (Zymo Research #D4014) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was amplified in two stages of Polymerase Chain Reaction (PCR) using Q5 DNA polymerase (NEB). For the first PCR reaction ...
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bioRxiv - Genomics 2023Quote: ... PCR master mix (64 µL of H2O, 100 µL of Q5 high-fidelity DNA polymerase (NEB #M0544S), 8 µL of i5 primer ...
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bioRxiv - Plant Biology 2024Quote: ... and the genes were PCR-amplified using Q5® High-Fidelity 2X Master Mix (New England Biolabs).
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bioRxiv - Biochemistry 2024Quote: Point mutants were generated using inverse polymerase chain reaction (PCR) with Phusion DNA polymerase (New England Biolabs) or Herculase II (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... in a thermal cycler with a 2 × Q5 PCR master mix (New England Biolabs, Ipswich, MA, USA). The following program was used ...
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bioRxiv - Genomics 2023Quote: ... Real-time polymerase chain reaction (PCR) was performed using Luna® Universal qPCR Master Mix (NEB M3003L) and the Bio-Rad CFX96 Touch Real-Time PCR Detection System (Bio-Rad 1845097) ...
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bioRxiv - Biophysics 2023Quote: ... The PCR product was spin column purified and then digested with BssSI-v2 and PpuMI (NEB R0560) in order to form the 6,481 bp center segment with 4 or 3 bp overhangs on the ends ...
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bioRxiv - Bioengineering 2023Quote: ... The inverse PCR protocol was adapted from an established protocol (56) utilizing Sau3AI (New England Biolabs®) and HinP1I (New England Biolabs® ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids with correct fragment size were amplified by PCR using Q5® High-Fidelity DNA polymerase (NEB) and integration site flanks (50 base pair homologous region ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplified products were screened by restriction digest analysis using the CviAII restriction enzyme (New England Biolabs). Positive candidates were sequenced to identify and confirm indels (Figure S6).
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bioRxiv - Immunology 2023Quote: ... The PCR thermal conditions were conducted using Q5® High-Fidelity DNA Polymerase (New England Biolabs, USA) at two step-PCR 98 °C 30 sec ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic PCR was carried out using NEBNext® Ultra™ II Q5® Master Mix (NEB, M0544S) to amply Mir96 loci ...
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bioRxiv - Neuroscience 2023Quote: ... 72°C – 1 min]) with NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, #M0541S) and barcoded Nextera primers (1.25 μM each ...
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bioRxiv - Bioengineering 2024Quote: ... 0.5 % Tween-20 and the selected genes amplified by PCR using Q5® Hot Start polymerase (NEB) using forward primer ...
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bioRxiv - Molecular Biology 2023Quote: ... PCC 7002 gDNA by PCR using the Syn0852-FULL-F6/R4 primers and Phusion DNA polymerase (NEB). The pET21c-GPR-EGFP vector was PCR-linearized with pET-F/R and pre-digested with HindIII-HF and XhoI (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR reactions were performed with Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs) in 20 µl reactions and products were run on a 1% agarose gel alongside an appropriately sized ladder (either New England Biolabs 100 bp or 1kb DNA ladder ...