Labshake search
Citations for New England Biolabs :
3551 - 3600 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2019Quote: ... PCR amplicons were then used for library preparation using NEBNext DNA Library Modules for Illumina platform (NEB). The final libraries were quantified using KAPA library quantification kit for Illumina (KAPA Biosystems) ...
-
bioRxiv - Genomics 2019Quote: ... PCR was performed using Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA, #M0530L) following the manufacturer’s instructions.
-
bioRxiv - Bioengineering 2021Quote: ... GFP and taCA were cloned by polymerase chain reaction (PCR) using pMAL-c5X (New England Biolabs, USA), pGEX-4T-1 (GE Healthcare ...
-
bioRxiv - Bioengineering 2019Quote: ... whereas colony PCRs were performed using 2xOneTaq Quick-Load Master Mix with Standard Buffer (New England Biolabs). Genomic DNA was extracted from overnight cultures using Yeast DNA Extraction Kit (Thermo Scientific) ...
-
bioRxiv - Microbiology 2021Quote: PCR amplifications were performed using OneTaq Quick-Load 2X Master Mix (New England BioLabs, Ipswich, MA, USA), 200 nM of each primer ...
-
bioRxiv - Cell Biology 2020Quote: ... Multiplex quantitative PCR was then performed using the Luna Universal Probe qPCR Master Mix (ref M3004, NEB), a Roche LightCycler or Rotor Gene Q real-time PCR (Qiagen ...
-
bioRxiv - Immunology 2021Quote: ... Remaining variants of BRD9 were generated by overlap-extension PCR using Q5 Hot Start High-Fidelity (NEB). TurboID-V5 constructs were generated by first subcloning TurboID from an existing vector (Addgene plasmid #10716949 ...
-
bioRxiv - Bioengineering 2020Quote: ... Positive clones were confirmed for the genotype by colony PCR using Q5 Polymerase enzyme (NEB, Ipswitch, MA). The dCpf1/CRISPRi system was adapted for use in P ...
-
bioRxiv - Neuroscience 2021Quote: ... The open reading frame was PCR-amplified (Phusion high-fidelity DNA polymerase, New England Biolabs Cat #M0530) using primers Irk1-F and Irk1-R (Supplemental Table 7) ...
-
bioRxiv - Cancer Biology 2020Quote: ... The PCR product was then end-repaired to produce blunt ends using T4 DNA polymerase (NEB M0203L), DNA Polymerase I ...
-
bioRxiv - Cancer Biology 2020Quote: ... The purified PCR products were denatured and reannealed in NEBuffer 2 (New England BioLabs, Ipswich, MA, USA), using a thermocycler ...
-
bioRxiv - Cell Biology 2021Quote: ... All ORF6 mutants were generated by PCR amplification using Phusion high fidelity DNA polymerase (NEB, Ipswich, MA) and overlapping PCR to introduce the indicated mutations ...
-
bioRxiv - Cell Biology 2021Quote: ... The PCR product was restricted with 66.7 U/ml AluI (R0137, New England Biolabs, Ipswich, MA, USA) in CutSmart buffer (New England Biolabs ...
-
bioRxiv - Microbiology 2020Quote: ... PCR reactions were set up with 1 μL of genomic DNA and either Q5 DNA Polymerase (NEB) or LongAmp Taq (NEB ...
-
bioRxiv - Plant Biology 2021Quote: ... Gel-extracted PCR products were assembled in a cut-ligation reaction via BsaI-HFv2 (New England Biolabs) into vector pRRES208.482 (kindly provided by Alison Huttly ...
-
bioRxiv - Plant Biology 2021Quote: ... The cDNA was used as a template for quantitative PCR using Luna Universal qPCR Master Mix (NEB) and a Viia7 real-time PCR instrument (Life Technologies/ABI) ...
-
bioRxiv - Plant Biology 2021Quote: ... and (iii) using 18 cycles of PCR amplification with Phusion High Fidelity DNA Polymerase (New England Biolabs). Libraries were pooled and sequenced (paired-end 2×150bp ...
-
bioRxiv - Microbiology 2020Quote: ... PCR reactions were performed with the Q5 Hot Start High-Fidelity 2× Master Mix (New England Biolabs) with 100 ng of genomic DNA ...
-
bioRxiv - Microbiology 2021Quote: ... and 20 ng of mRFP PCR products were incubated with Gibson master mix (New England Biolabs, #E2611S) at 50°C for 1 hour ...
-
bioRxiv - Genomics 2021Quote: ... Carrier plasmid and the PCR product from the last step were digested with BsmBI (NEB, buffer 3.1) at 55°C for 1h and were purified for ligation ...
-
bioRxiv - Microbiology 2021Quote: ... Two microlitres of this reaction was then used as template for PCR using Taq DNA polymerase (NEB), DNA oligo E4 (CSO-4720) ...
-
bioRxiv - Microbiology 2020Quote: ... The digested hemA PCR product and pHSG396 were ligated together using T4 ligase (New England Biolabs, US), at a 3:1 insert to vector mass ratio ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR was performed using a Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs) and the cycling conditions were ...
-
bioRxiv - Cell Biology 2020Quote: ... Nested PCR was then carried out using OneTag DNA polymerase with GC buffer and Q5 polymerase (NEB) for genomic DNA and cDNA ...
-
bioRxiv - Molecular Biology 2022Quote: ... 12.5 µl of Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB, Catalog no. M0531S) 0.8 µM of each primer (final concentration ...
-
bioRxiv - Cancer Biology 2022Quote: ... sgRNA sequences were then PCR-amplified using Q5 Hot Start High-Fidelity 2X Master Mix (M0494, NEB), barcoded primer pairs (see Supplemental Table 2 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 200 mg of DNA per condition were PCR amplified using Q5 high fidelity DNA polymerase (NEB Inc.) for 16 cycles using primers (Forward ...
-
bioRxiv - Cell Biology 2022Quote: ... All purified DNA (~20 µl) was used for PCR amplification with NEBNext High Fidelity 2x MasterMix (NEB) and optimum cycle number was determined by qPCR ...
-
bioRxiv - Biochemistry 2022Quote: ... The amplified PCR product was digested in one step using NdeI and BamHI HF (New England Biolabs) and ligated into similarly treated pET-15b to create plasmid pET15b-EcGhrA ...
-
bioRxiv - Biophysics 2022Quote: ... The pooled PCR products were incubated at a 10:1 ratio with DpnI enzyme (New England Biolabs) at room temperature for 5 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... Beads were resuspended in 25 μl cDNA amplification mix (1X Phusion HF PCR Master Mix (NEB, #M0531L) and 0.5uM P5 and P7 short primer mix (see Supplementary Table 2) ...
-
bioRxiv - Immunology 2022Quote: ... PCR amplification was performed using the Q5® Hot Start High-Fidelity 2X Master Mix (NEB, M0494) following the protocol provided by the manufacturer ...
-
bioRxiv - Genomics 2022Quote: Selected Aß42 variants for individual testing were obtained by PCR linearisation (Q5 high-fidelity DNA polymerase, NEB) with mutagenic primers (primers MS_38-47 ...
-
bioRxiv - Microbiology 2022Quote: ... The PCR reaction to simultaneously amplify all eight gene segments was performed using Phusion polymerase (NEB #M0530L) as follows ...
-
bioRxiv - Microbiology 2022Quote: ... Subsequent on-bead PCR indexing-amplification of tagmented DNA was performed using 2x Phusion Master Mix (NEB) and custom-ordered indexing primers (IDT ...
-
bioRxiv - Cancer Biology 2022Quote: ... PCR fragments were gel purified and equal amounts of DNA were subject to DrdI (New England Biolabs) enzyme digest following manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... These fragments were PCR-amplified by Q5® Hot Start High-Fidelity DNA Polymerase (New England Biolabs), visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) ...
-
bioRxiv - Microbiology 2022Quote: 50 μl PCR reactions were performed using Q5 Hot start High-Fidelity DNA polymerase (New England Biolabs), PJF119EH-cusF (pAV54 ...
-
bioRxiv - Neuroscience 2020Quote: ... target site flanking sequences of approximately 1kb size were amplified by PCR (Q5 polymerase, New England Biolabs) from genomic DNA of nos-Cas9[attP2] fly line used for embryo injection ...
-
bioRxiv - Plant Biology 2021Quote: ... DNA fragments were PCR-amplified for 19 cycles using the NEB 2X Taq MasterMix (NEB, MA, USA) and 35 ng of starting DNA in an Eppendorf Mastercycler ep gradient S (Eppendorf ...
-
bioRxiv - Neuroscience 2020Quote: ... PCR(20 total cycles) was then carried out using the Q5 Ultra II Q5 Master Mix(NEB) to amplify final libraries using universal Illumina primers ...
-
bioRxiv - Microbiology 2020Quote: ... PCR enrichment of adaptor-ligated DNA was performed using NEBNext Multiplex Oligos for Illumina (New England Biolabs) to produce Illumina-ready libraries ...
-
bioRxiv - Microbiology 2021Quote: ... and the PCR fragment of the plasmid were assembled together using the Hifi DNA assembly mix (NEB). The construction was verified by sequencing.
-
bioRxiv - Bioengineering 2021Quote: A standard PCR process was performed using Q5 high-fidelity DNA polymerase from NEB (cat. no. M0491S). All PCR primers were purchased from IDT (Supplementary Table S1) ...
-
bioRxiv - Biophysics 2021Quote: ... Two of them correspond to two PCR-fabricated DNAs (C1 and C2) derived from lambda DNA (NEB) as template and including suitable restriction sites in the designed primers (Table S1) ...
-
bioRxiv - Microbiology 2022Quote: ... PCR to amplify constructs for cloning and sequencing were performed using Q5 High-Fidelity DNA polymerase (NEB), OneTaq DNA polymerase (NEB) ...
-
bioRxiv - Evolutionary Biology 2022Quote: PCR reactions were done with the Phusion high fidelity DNA polymerase (New England Biolabs Canada, Whitby Ontario) using standard protocols ...
-
bioRxiv - Genomics 2022Quote: ... All PCR reactions were performed using Q5® Hot Start High-Fidelity 2X Master Mix (NEB, M0494S). The in vitro transcription of VRQR-ABE7.10max mRNA reaction contains 50 ng/μL linearized VRQRABE-mRNA DNA template ...
-
bioRxiv - Synthetic Biology 2019Quote: ... were assembled by PCR using the Phusion® High-Fidelity DNA Polymerase (New England Biolabs, MA, USA) and the mCherry_KpnI_fw and mCherry_KpnI_rv primers (Supplementary Table 4) ...
-
bioRxiv - Neuroscience 2019Quote: ... HDAC6 cDNA was generated from pCMV6-XL4-HDAC6 by PCR using Phusion High Fidelity DNA polymerase (NEB) with primers containing restriction sites (SalI-HDAC6-forward ...