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Citations for New England Biolabs :
3401 - 3450 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 10 μl PCR products were incubated with 1.5 μl 10X NEBuffer (New England Biolabs, Ipswich, MA) and 1.5μl Nuclease-free water with the thermal cycler setting ...
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bioRxiv - Molecular Biology 2023Quote: Purified samples were combined with NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs M01541) for amplification ...
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bioRxiv - Plant Biology 2023Quote: ... Amplifications were performed using the High-Fidelity Phusion PCR Master Mix (NEB, Frankfurt am Main, Germany) or the GoTaq G2 polymerase (Promega ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 μl was used for PCR amplification using Q5 hot start high-fidelity polymerase (NEB #M0494S) and a unique combination of the dual-barcoded primers P5 and P7 Nextera XT Index kit (Illumina #15055293) ...
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bioRxiv - Molecular Biology 2022Quote: ... we used the 2X Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs), and 300 ng of the cDNA and 10 µM of each the RSV5U and RTRHC primers ...
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bioRxiv - Molecular Biology 2022Quote: ... A 50 μL PCR reaction for the gene block was carried out with Phusion polymerase (NEB) using primers DGR_gblock_fwd and DGR_gblock_rev for 35 cycles (denaturing ...
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bioRxiv - Molecular Biology 2022Quote: ... the adaptor-ligated cDNA was processed to library PCR amplification using Phusion Master Mix (NEB, M0531L) with IDT adaptor indexes ...
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bioRxiv - Microbiology 2022Quote: Putative replicon fragments were obtained through amplification PCR using high fidelity enzyme Q5 DNA polymerase (NEB) and primers including a KpnI or NcoI restriction site (Table 2) ...
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bioRxiv - Microbiology 2022Quote: ... PCRs were performed using specific primers (Table S2C) and Q5 High- Fidelity Polymerase (NEB, Hitchin, UK) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... This made DNA accessible for PCR amplification with barcoded primers for Illumina sequencing (NEB, cat# E7335L). PCR amplifications were carried out for 16 cycles [98 °C 30 s ...
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bioRxiv - Molecular Biology 2022Quote: ... Digested and purified PCR products were then phosphorylated and ligated simultaneously using T4 PNK Enzyme (NEB), T4 DNA Ligase (NEB) ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting PCR fragment was gel-purified and was blunt-end-ligated into the Eco53kI (NEB) site of broad host-range vector pBBR1MCS-5 (Kovach et al. ...
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bioRxiv - Bioengineering 2022Quote: ... and amplified in 50 µl PCR reactions using Taq DNA polymerase (NEB, Cat. No. / ID: #M0273S) and primers 1154A.S8 and 1154A.S29 (1121bp) ...
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bioRxiv - Biophysics 2022Quote: ... miRFP670nano replaced EGFP and EGFPA206K mutated back into EGFP using PCR and Gibson Assembly System (NEB). Cell transfection was done with PEI reagent (24765-1 ...
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bioRxiv - Cell Biology 2024Quote: ... Then the PCR amplicon of Cdk1 CpG was digested by KpnI and MfeI (New England Biolabs) to remove the overhangs ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were inserted into the respective plasmids using the NEBuilder HiFi DNA Assembly system (NEB). All constructs used in this study were confirmed by sequencing ...
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bioRxiv - Bioengineering 2024Quote: ... comprising 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA), 0.2 μM of forward and reverse primers ...
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bioRxiv - Bioengineering 2024Quote: Q5 DNA Polymerase was used for DNA amplification and colony PCR screening (New England Biolabs, Germany). Gibson Assembly and site-directed mutagenesis was performed using the NEBuilder HiFi DNA Assembly Cloning Kit (E5520S ...
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bioRxiv - Cancer Biology 2024Quote: ... ATAC-seq libraries were prepared using the NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541) as previously described76 ...
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bioRxiv - Cancer Biology 2024Quote: RNAs were transcribed from 1 µg of PCR-amplified templates using T7 RNA polymerase (NEB M0251S) for 2 h at 37 °C according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR was done using intron-specific outward pointing primers (Table S7) and Phusion DNA polymerase (NEB). Purified PCR products were incubated with Taq to add a 3’ A residue and cloned using a Topo cloning kit (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product and the vector were enzyme-digested using NdeI and XbaI (New England Biolabs) for 2 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR products (∼300 ng) were incubated with 2 μl 10X NEBuffer 4 (New England Biolabs), 1 μl BtsCI restriction enzyme ...
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bioRxiv - Molecular Biology 2024Quote: ... The linearized vector was then ligated to the digested PCR product using a quick ligase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Homo sapiens cDNAs were amplified by PCR using Q5 High Fidelity DNA Polymerase (New England Biolabs) and sub-cloned into a variety of vector backbones as indicated in the figure legends ...
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bioRxiv - Microbiology 2024Quote: ... Flanking regions of the target genes were amplified via PCR using Phusion polymerase (New England BioLabs). All primers used in the study are listed in Table S7.
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bioRxiv - Microbiology 2024Quote: ... the linear plasmid obtained by PCR was ligated using the KLD enzyme mix (NEB cat # M0554S) according to the manufacturer’s protocol before transformation ...
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bioRxiv - Immunology 2024Quote: ... DNA was then amplified using NEBNext High Fidelity 2X PCR Master Mix (New England Biolabs, #M0541S) for 5 cycles ...
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bioRxiv - Cell Biology 2023Quote: ... 2µg of resulting PCR products were then digested with Pst1-HF restriction enzyme (New England Biolabs) to cleave the unspliced XBP1 template ...
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bioRxiv - Cell Biology 2023Quote: ... Mutagenesis on Cas9 and cGAS was performed by conventional PCR followed by HiFi DNA Assembly (NEB) or In-Fusion cloning (Takara Bio) ...
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bioRxiv - Immunology 2023Quote: ... for mouse was PCR-amplified and ligated into this vector via Gibson assembly (NEB, Cat# E2621S). The ligated product was precipitated ...
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bioRxiv - Genomics 2023Quote: ... Homology arms were amplified from HaCat cell genomic DNA via PCR using Q5 DNA polymerase (NEB). The −loxP-PGK-Em7-NeoR-loxP- cassettes were amplified from PL452 (79) ...
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bioRxiv - Biochemistry 2022Quote: ... pDONR/Zeo plasmid was linearized via Polymerase Chain Reaction (PCR) with the Q5 DNA polymerase (NEB) and a pair of primers for each Cac1 mutant that anneal to the sequences flanking the section to be modified (Supplementary Table 2) ...
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bioRxiv - Cell Biology 2023Quote: ... HindIII/NotI-digested PCR reactant and backbone were ligated by T4 DNA ligase (New England Biolabs) and transformed to CloneCatcher™ (Genlantis ...
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bioRxiv - Immunology 2023Quote: Phage DNA was subjected to two rounds of PCR using Q5 High-Fidelity 2X Mastermix (NEB), as previously described (Garrett et al. ...
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bioRxiv - Immunology 2023Quote: ... and 100-200ng were PCR amplified using Q5 Hot Start High Fidelity 2x Master Mix (NEB) and 10µM forward/reverse primers flanking the region of interest ...
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bioRxiv - Genomics 2023Quote: ... The region containing the linker-mAID sequence was then amplified by PCR using Phusion polymerase (NEB) from the modified pMK287-mAID-Hygro plasmid and subcloned into the pL452 vector which harbors the Neomycin resistance cassette under the control of a PGK promoter ...
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bioRxiv - Bioengineering 2023Quote: ... In brief, DNA fragments were amplified by PCR (Q5 2x Master Mix, New England Biolabs (NEB)) ...
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bioRxiv - Cell Biology 2023Quote: ... The resulted PCR product co-transformed with pEGFP-C1-2µ-URA3 cut with BamHI (NEB, R3136) into yeast strain BY4743 (EUROSCARF Y20000 ...
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bioRxiv - Cancer Biology 2023Quote: ... TCRs were then amplified by PCR (20 cycles with the Phusion from New England Biolabs, NEB) with a single primer pair binding to the constant region and the adapter linked to the TRAV/TRBV primers added during the reverse transcription ...
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bioRxiv - Cancer Biology 2023Quote: ... TCRs were then amplified by PCR (20 cycles with the Phusion from New England Biolabs, NEB) with a single primer pair binding to the constant region and the adapter linked to the TRAV/TRBV primers added during the reverse transcription ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR was performed on genomic DNA by using Q5 High-Fidelity Taq Polymerase (New England Biolabs) followed by T7 endonuclease I assay (New England Biolabs ...
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bioRxiv - Developmental Biology 2023Quote: ... The PCR products were used as templates for transcription reactions using T3 RNA polymerase (Biolabs, M0378S) or T7 RNA polymerase (TOYOBO ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were then isolated via gel electrophoresis and assembled using HiFi assembly mix (NEB #E2621L). Plasmids were verified by DNA sequencing and stored at -20°C until use in NanoBRET assays.
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bioRxiv - Bioengineering 2023Quote: ... plasmid pJC1-trpE (7749 bp) was amplified by PCR using oligonucleotides with degenerated (NNK) codons (NEB Q5 Site-Directed-Mutagenesis Kit ...
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bioRxiv - Biochemistry 2023Quote: DNA templates for the transformation assays were prepared by PCR amplification from the pUC19 plasmid (NEB), after which the PCR fragments were Dpn1-treated ...
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bioRxiv - Biochemistry 2023Quote: The PCR product was purified and 1 µg was phosphorylated using 10 units T4 PNK (NEB) in 20 µl of T4 ligase buffer (NEB ...
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bioRxiv - Genetics 2023Quote: ... All PCR amplifications were done using Q5® High-Fidelity DNA Polymerase (New England Biolabs, UK) following manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR enrichment step was performed with NEBNext Multiplex Oligos for Illumina (Dual Index Primers, NEB #E7600), using 64 different combinations of indexed primers ...
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bioRxiv - Cancer Biology 2023Quote: ... Standard cloning was performed using PCR (35 μL ddH2O, 10 μL 5x HF Phusion buffer (NEB), 1 μL of 10 mM mixed dNTPs ...