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Citations for New England Biolabs :
1851 - 1900 of 4746 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: All Gibson assemblies were performed using NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) and were assembled at 50 °C for 60 min at appropriate molar ratios ...
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bioRxiv - Cancer Biology 2020Quote: Libraries were prepared using NEBNext® ChIP-Seq Library Prep Master Mix (E6240, NEB) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S ...
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bioRxiv - Plant Biology 2020Quote: ... The fragment was re-assembled using NEBuilder® HiFi DNA Assembly Master Mix (NEB). Constructs were transformed into BL21(DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... Libraries were then amplified with Q5 High-Fidelity Master Mix (M0492S, NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Systems Biology 2021Quote: 25 ul - Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs)
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bioRxiv - Microbiology 2021Quote: ... All PCRs of flanking regions were carried out using Phusion-HF Master Mix (NEB) according to the manufacturer’s protocol with an annealing temperature (Ta ...
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bioRxiv - Cell Biology 2021Quote: ... and fused with Gibson recombination cloning method (Gibson Assembly Master Mix, NEB, UK, #E2611) in pEGFP-N3 empty backbone ...
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bioRxiv - Microbiology 2021Quote: ... by Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, USA). This vector was named pDAR25-Kan ...
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bioRxiv - Plant Biology 2020Quote: ... using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs Inc., UK, pE2B-MpC1HDZmut). Later ...
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bioRxiv - Genomics 2021Quote: ... The library was assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) in 4 × 20 μL reactions at 50 °C for 1 hour using 100 ng of vector per 5-10 ng of PCR amplicon ...
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bioRxiv - Cell Biology 2021Quote: ... DNA constructs were either generated using the Gibson Assembly Master Mix (New England Biolabs), or the T4 DNA ligase (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... and cloning was performed using Gibson Assembly 2× Master Mix (New England BioLabs, NEB) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and cloning was performed using Gibson Assembly 2× Master Mix (New England BioLabs, NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The reactions were performed using the Multiplex 5X Master Mix (New England Biolabs, Inc.) in a final volume of 12.5 μl following the protocol described by the manufacturer ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled withGibson assembly protocol using NEBuilder HiFi DNA Assembly Master Mix(NEB, E2621S). Assembled DNA was transformed into competent cells prepared by the CCMB80 buffer (TekNova ...
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bioRxiv - Synthetic Biology 2022Quote: Constructs were assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB, catalogue no. E2621L). Reactions consisting of approximately equal amounts of the common fragment and the variable fragment were prepared using the FeliX liquid handler or electronic multi-channel pipette ...
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bioRxiv - Microbiology 2022Quote: ... Amplicons were detected with Luna® Universal qRT-PCR Master Mix (New England Biolabs) in a CFXConnect Real-Time PCR Instrument (Bio-Rad Laboratories) ...
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bioRxiv - Molecular Biology 2020Quote: All plasmids were cloned using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) and mutations were made using the Q5 Site-directed mutagenesis kit (New England Biolabs) ...
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bioRxiv - Bioengineering 2021Quote: ... Reactions were performed with Phusion High-Fidelity Master Mix and GC buffer(NEB, US) using 1 to 30 ng of plasmid DNA templates ...
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bioRxiv - Synthetic Biology 2022Quote: ... by isothermal assembly using the HiFi DNA Assembly Master Mix (New England Biolabs, USA). Following assembly ...
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bioRxiv - Cell Biology 2019Quote: ... Library was amplified using NEBNext High-Fidelity 2× PCR Master Mix (New England Biolabs). Library concentration was measured by DNA High Sensitivity Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... and cloned into an SEC backbone plasmid (NEBuilder HiFi DNA Assembly Master Mix, NEB). The modified Cas9/sgRNA plasmid ...
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bioRxiv - Synthetic Biology 2020Quote: ... 6 μl primers (10 μM) and 50 μl Q5 high fidelity master mix (NEB). The thermocycling parameters were ...
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bioRxiv - Microbiology 2019Quote: ... Libraries were constructed using the NEBNext Ultra DNA Sample Prep Master Mix Kit (NEB), with minor modifications and a custom automated protocol on a Biomek FX (Beckman) ...
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bioRxiv - Genomics 2021Quote: ... (Grevet et al., 2018) using NEBuilder HiFi DNA Assembly master mix (NEB, Cat #E2621X) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... which included 25 µL of NEBNext Ultra II Q5 master mix (NEB, Cat #M0544L), 1.25 µL of the 10 µM forward primer and 1.25 µL of the 10 µM reverse primer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or NEBNext Q5 HotStart HiFi PCR Master Mix (New England BioLabs, Ipswich, MA, USA) for eight to 14 cycles ...
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bioRxiv - Cell Biology 2020Quote: ... effectors were then inserted into the vector using the Gibson Assembly Master Mix (NEB).
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bioRxiv - Physiology 2020Quote: ... was amplified by PCR (Phusion High-Fidelity PCR Master Mix, NEB, Hitchin, Hertfordshire, UK) using specific oligonucleotide primers (5’-TCGCTACTGTTTCTCTCGCA-3’ and 5’-AAAGGCGTCAACAACTGCTT-3’) ...
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bioRxiv - Biophysics 2020Quote: ... The two DNAs were ligated using a Gibson Assembly Master Mix (New England Biolabs) and subcloned into the pWMD01 vector (33) ...
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bioRxiv - Cell Biology 2021Quote: ... all sgRNAs were amplified using Phusion Flash High-Fidelity PCR Master Mix (NEB, M0531L) with the primers ...
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bioRxiv - Cell Biology 2020Quote: ... The donor cassettes were assembled using the NEBuilder HiFi DNA Assembly Master Mix (NEB) and were designed to leave the 5’UTR and 3’UTR of the Copg1 gene intact ...
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bioRxiv - Microbiology 2021Quote: ... and inserted to the intermediate plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB). The L-sNTurboID fragment was ligated back to the pCEZ-L backbone using the same restriction sites Pac1 and Hpa1 by NEB T4 ligase.
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bioRxiv - Molecular Biology 2021Quote: ... or fragment assembly using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and NEBuilder HiFi DNA Assembly Master Mix (E2621L, New England BioLabs, Ipswich, MA, USA). All constructs were verified by Sanger sequencing and midiprepped for electroporation and/or transfection using PureLink Fast Low-Endotoxin Midi Kit (A36227 ...
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bioRxiv - Biophysics 2020Quote: ... The resulting PCR product was subsequently inserted using Gibson Assembly Master Mix (NEB, E2611L) into the lentiviral vector CD813A (System Biosciences ...
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bioRxiv - Genomics 2020Quote: ... and RT-PCR was performed with Phusion High Fidelity DNA polymerase master mix (NEB) each with the manufacturer recommended PCR thermal cycles ...
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bioRxiv - Genomics 2020Quote: ... Samples were dissolved in 10 μl of master mix for fragmentation (consisting of NEB next First Strand Synthesis ...
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bioRxiv - Neuroscience 2021Quote: ... RILPL1-miniSOG-HA construct was generated using Gibson Assembly Master Mix (New England Biolabs), and identity of construct verified by sequencing of the entire coding region ...
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bioRxiv - Microbiology 2021Quote: ... using NEBuilder High Fidelity (HiFi) DNA Assembly Master Mix (New England Biolabs, Ipswich, MA) via Gibson Assembly Protocol (68) ...
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bioRxiv - Genetics 2020Quote: ... amplified using primers with KpnI and BamHI RE sites with Phusion Master Mix (NEB), digested ...
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bioRxiv - Genomics 2021Quote: ... Tables S1 and S7) using NEBNext Ultra II Q5 Master Mix (New England Biolabs). Each reaction (100 µL total volume ...
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bioRxiv - Genomics 2020Quote: ... Libraries were constructed using the NEBNext Ultra DNA Sample Prep Master Mix Kit (NEB) with minor modifications and a custom automated protocol on a Biomek FX (Beckman)25 ...
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bioRxiv - Cell Biology 2021Quote: ... cloned into NdeI cut pET28a using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621S), single colonies picked and Sanger sequenced.
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bioRxiv - Cancer Biology 2021Quote: ... using the NEBuilder Hi Fi DNA Assembly Master Mix (New England Biolabs, Beverly, MA). For the production of lentiviral particles ...
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bioRxiv - Cell Biology 2022Quote: ... The cloning reactions were performed using the Gibson Assembly Master Mix (New England Biolabs). Site-directed mutagenesis was performed with the Quik-Change kit (Agilent) ...
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bioRxiv - Microbiology 2022Quote: ... both fragments being assembled with NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Catalytic mutants msrDE125Q ...
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bioRxiv - Genomics 2022Quote: ... The enriched DNA fragments were further amplified using NEBNext Q5 Master Mix (NEB, M0544) and NEBNext Library Quant Primer Mix (NEB ...
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bioRxiv - Genomics 2022Quote: ... then ligated to a custom hairpin adapter using NEB ligase master mix (NEB, M0367). The incomplete ligation product (fragment having only one or no adaptor ligated ...
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bioRxiv - Microbiology 2022Quote: ... Gibson reactions were carried out according to manufacturer’s recommendations (NEB, Gibson Assembly master mix). This was transformed into electrocompetent E ...