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Citations for New England Biolabs :
1901 - 1950 of 4746 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... and self-assembled using Gibson assembly (NEBuilder® HiFi DNA Assembly Master Mix, NEB).
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bioRxiv - Microbiology 2022Quote: ... and 25 μL of NEB Blunt/TA Ligase Master Mix (New England Biolabs, UK). The mixture was incubated at room temperature for 10 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB, 1 h 50° C) into backbones previously digested with corresponding restriction enzymes (NEB ...
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bioRxiv - Microbiology 2022Quote: ... Secondary amplification reactions were performed using NEBNext high-fidelity 2 PCR master mix (NEB) in 50 μl reactions (26 μl of 2X mix ...
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bioRxiv - Cell Biology 2022Quote: ... sgRNA inserts were PCR-amplified using NEBNext High Fidelity PCR Master Mix (NEB, M0541). The resulting PCR amplicons were purified using Ampure XP Beads and sequenced using the HiSeq 2500 system (Illumina ...
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bioRxiv - Microbiology 2022Quote: The second cDNA strands were generated with LongAmp Taq Master Mix (New England Biolabs). The ends of the double-stranded cDNAs were repaired with NEBNext End repair /dA-tailing Module (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR was performed using the Quick-LoadR©Taq2×Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using Phusion 5X Master mix (New England Biolabs GmbH, M0492S). For a 25 µl reaction ...
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bioRxiv - Biochemistry 2023Quote: ... Cloning was performed using either NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) or standard restriction cloning methods ...
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bioRxiv - Neuroscience 2023Quote: ... Gibson Assembly was performed using NEB HiFi Gibson Assembly Master mix (NEB Catalog# E2621L). Gibson Assembly reaction products were transformed into NEB Turbo high efficiency chemically competent cells (NEB Catalog# C2984I ...
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bioRxiv - Neuroscience 2023Quote: ... Gibson Assembly was performed using NEB HiFi Gibson Assembly Master mix (NEB Catalog# E2621L). The scFv Gibson Assembly reaction products were transformed into NEB Turbo high efficiency chemically competent cells (NEB Catalog# C2984I ...
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bioRxiv - Synthetic Biology 2023Quote: ... Reactions closely followed instructions from the NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621S). Fragments were amplified with PCR and designed to have 20-30 bp overlaps ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed utilizing the Phusion High-Fidelity PCR Master Mix (NEB, M0531L) to prevent any mutations ...
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bioRxiv - Microbiology 2023Quote: ... and ligated into pET17b vector using the Gibson Assembly Master Mix (New England Biolabs). The ligation products were transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... and ligated into the pET17b plasmid randomly using the Gibson Assembly Master Mix (NEB). The transformation was performed in the same manner as the above.
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bioRxiv - Immunology 2023Quote: ... tagmented DNA was amplified using NEBNext High-Fidelity PCR Master Mix (New England BioLabs) with the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... Assembly was done with NEBuilder HiFi DNA Assembly Master Mix (E2621L, NEW ENGLAND BioLabs). Correct insertion of the amplicon was confirmed by sequencing ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs catalog # M0531), and/or LongAmp® Hot Start Taq DNA Polymerase with Expand HF Buffer (Roche catalog # 05917131103 ...
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bioRxiv - Genomics 2022Quote: ... 16 µl RNA were mixed with 4 µl LunaScript master mix (NEB cat# E3010L), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... using NEBuilder HiFi DNA Assembly Master Mix (#M5520AA, New England Biolabs, Ipswich, MA, USA). For the construction of the SNV-Env expression plasmid (phCMV3-SNV-Env) ...
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bioRxiv - Genetics 2023Quote: ... 5% DMSO and 1× NEBNext High-Fidelity PCR master mix (New England BioLabs, M0541L) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and subjected to Gibson Assembly using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621L) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... PCR reactions were performed with Phusion High-Fidelity PCR Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... flanked by GreenGate-compatible ends using Gibson Assembly® Master Mix – Assembly (NEB, E2611). The resulting binary vectors pGGSun ...
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bioRxiv - Biochemistry 2023Quote: ... and for colony PCRs the OneTaq Quick-Load Master Mix Polymerase was used (NEB). PCR products were analyzed on 1 % TAE agarose gels ...
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bioRxiv - Bioengineering 2023Quote: ... All ligations were performed via HiFi master mix two-fragment assemblies (New England Biolabs). The assembled pMTL71301 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was performed using Phusion High-Fidelity PCR Master Mix (New English Biolabs, England). PCR products were quantified using 2% agarose gel electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µM of each primer was combined with OneTaq QuickLoad Master Mix (NEB #M0486) and run in a thermocycler as follows ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PCR reactions contained 1X Phusion High-Fidelity PCR Master Mix (New England BioLabs Inc.), 0.5 μM primers (forward ...
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bioRxiv - Biochemistry 2023Quote: ... The final plasmids were then constructed using Gibson Assembly Master Mix (NEB, Cat # E2611S), merging the amplified gene fragments with the NotI/XmaI digested parent vector.
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bioRxiv - Microbiology 2023Quote: ... PCR amplicons were obtained using the Q5 High-Fidelity Master Mix (New England, BioLabs), 0.4 μM each primer ...
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bioRxiv - Genomics 2023Quote: ... The homology vector was cloned by Gibson assembly (Gibson Assembly Master Mix kit, NEB) into a restriction-enzyme digested pCAG backbone plasmid ...
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bioRxiv - Developmental Biology 2024Quote: ... and introduced to pCS2+ vector using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621). The H3-tail sequence (Shindo & Amodeo ...
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bioRxiv - Immunology 2024Quote: ... sample amplification was performed using a library PCR using Q5 Master Mix (M0544L, NEB), 1 µL i7 Index primer (Sigma-Aldrich®) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were purified and cloned using Gibson assembly master mix (New England BioLabs) into LRG3.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... For PCRs we used Phusion High-Fiedlity PCR Master Mix with HF Buffer (NEB) and reactions were run as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and pNJ-26 vector were assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB), to generate the plasmid pSAG1:EGFP-DHFR-BAG1:mCherry ...
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bioRxiv - Molecular Biology 2023Quote: ... Linearized cDNA was PCR amplified using Phusion High-Fidelity PCR Master Mix (NEB, M0531S).
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bioRxiv - Bioengineering 2024Quote: ... 500 µL PCR reagents containing 1.67X Q5® High-Fidelity Master Mix (NEB, M0515), 0.625 mg/mL BSA ...
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bioRxiv - Genetics 2023Quote: ... Plasmid assemblies were achieved either with NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621) or T4 DNA Ligase (M0202) ...
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bioRxiv - Genomics 2024Quote: ... a SYBR green I based qRT-PCR kit (Luna Universal One-Step NEB, Ipswich, USA) was used in PCR mixtures (20 µl ...
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bioRxiv - Immunology 2021Quote: ... Add 30 μl PCR master mix (2.5 μl Illumina dual indexes primer 1, 2.5 μl Illumina dual index primer 2, 25 μl NEB Q5 HotStart Master Mix) to the 20 μl beads bound with samples ...
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bioRxiv - Genetics 2022Quote: ... The reaction mixture was composed of a PCR-ready mix (using NEBNext® Ultra™ II Q5® Master Mix, NEB, M0544L), a cell lysis product ...
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bioRxiv - Molecular Biology 2023Quote: ... Adapter ligation was performed at 20°C for 10 min, with Adapter Mix (AMX, ONT) and Blunt/TA Ligase Master Mix (New England BioLabs, cat# M0367). After a final 1X clean-up and washing of the beads with Adapter Binding Buffer (ABB ...
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bioRxiv - Genetics 2023Quote: ... PCR was then performed in a mix of 10 µM Nextera i7 and i5 primers and NEBNext Q5 High-Fidelity PCR Master Mix (New England Biolabs, MA, USA) according to the following protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... MgCl2 was added to a final concentration of 50mM and 12.2µl of each tagmented sample was PCR amplified for 15 cycles using 2x NEB Next HiFi PCR mix (NEB). Amplified libraries were purified using the Qiagen PCR purification MinElute kit.
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bioRxiv - Developmental Biology 2021Quote: ... MgCl2 was added to a concentration of 50mM and 16µl of each sample were PCR amplified using 2x NEB Next HiFi PCR mix (NEB) and 16 PCR cycles.
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bioRxiv - Genomics 2021Quote: ... We amplified libraries by adding 10 ul DNA to 25 ul NEBNext HiFi 2x PCR mix (New England Biolabs) and 2.5 ul of a 25 uM solution of each of the Ad1 and Ad2 primers ...
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bioRxiv - Microbiology 2020Quote: ... Gibson assembly protocol using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA) was followed to ligate the VP1456 or the VPA0356 fragment with the expression vector pBAD33 ...
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bioRxiv - Cell Biology 2020Quote: ... The final vector was then assembled using the Gibson assembly master mix (New England Biolabs) and 2 overlapping fragments containing the T2A sequence and mCherry generated by PCR using the following primers and templates (see ‘Primer and sgRNA sequences’):