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Citations for New England Biolabs :
151 - 200 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: The exonuclease T degradation assays were performed as described by the manufacturer (New England BioLabs). Reactions (20 μl ...
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bioRxiv - Developmental Biology 2024Quote: ... cutting efficiency was determined by T7-endonuclease assay (T7 Endonuclease I, New England Biolabs, M0302) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... amplifications were submitted to cleavage assays using the T7 Endonuclease 1 (T7E1) enzyme protocol (NEB). PCR products of confirmed G2 heterozygous (n = 48 ...
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bioRxiv - Biochemistry 2024Quote: ... in a black-bottomed 96-well plate and gene-specific LA qPCR assays were performed as described earlier18,19,20,27,47,48 using Long Amp Taq DNA Polymerase (New England BioLabs). Three transcribed (HPRT ...
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bioRxiv - Microbiology 2023Quote: ... Monarch kit (NEB) extracted and 0.1 pmol of each DNA fragment was CPER amplified in a 50 µL reaction containing 200 µM of dNTPs ...
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bioRxiv - Genomics 2020Quote: ... The membrane fraction was then incubated with one of the three deglycosylation enzymes: O-Glycosidase (New England Biolabs, #P0733), PNGase F (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were dialyzed with water on silica membranes (0.025 μm pores) for 1 hour before transformed into DH10β cells (New England Biolabs). Library sizes of at least 100,000 colony-forming units (CFU ...
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bioRxiv - Biophysics 2024Quote: ... Reactions were dialyzed with water on silica membranes (0.025-μm pores) for 1 h before transformed into DH10B cells (New England Biolabs). Library sizes of at least 100,000 colony-forming units (cfu ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 20 pmol membrane permeable SNAP dye (10 μM SNAP-Cell® 647-SiR, New England BioLabs Inc.) in reconstitution buffer supplemented with 1 mM DTT or with each dye separately ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrations measured using Qubit 1X dsDNA HS Assay) was treated with 5U of RNAse HI (NEB) in RNAse HI buffer for 30 min at 37°C and then nucleic acids were purified with GeneJET Gel Extraction and DNA cleanup micro kit (General cleanup protocol ...
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bioRxiv - Microbiology 2021Quote: ... The assay mixture was then analyzed using western blotting by anti-PanKcr (PTM-501, PTM Biolabs) and anti-H3 (ab1791 ...
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bioRxiv - Microbiology 2022Quote: PCR was performed using colonies from transfer assays using OneTaq (New England BioLabs Inc., Ipswich, MA). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... The guide RNA target regions were amplified from transfected cells and analyzed by T7endo1 assay (NEB) to detect genome editing activity at the target site ...
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bioRxiv - Biochemistry 2023Quote: DNA templates for the transformation assays were prepared by PCR amplification from the pUC19 plasmid (NEB), after which the PCR fragments were Dpn1-treated ...
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bioRxiv - Developmental Biology 2024Quote: ... All constructs generated in this study were obtained using the Gibson assembly assay (NEB, Cat # E2611S).
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bioRxiv - Genomics 2022Quote: ... w/o any promoter for promoter-assays were first digested using EcoRV (New England Biolabs, R3195S). Next ...
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bioRxiv - Bioengineering 2024Quote: Protein was expressed for in vitro assays in T7 Express cells (New England Biolabs, Catalog # C2566H) in 96-well deep-well plates using the pET22b(+ ...
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bioRxiv - Neuroscience 2021Quote: ... T7 HiScribe kit (NEB) was used to transcribe the sgRNA DNA template with the appended T7 promoter ...
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bioRxiv - Cell Biology 2024Quote: ... The LunaScript Kit (NEB) was used for complementary DNA (cDNA ...
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bioRxiv - Biochemistry 2024Quote: PURExpress ΔRF123 kits (NEB) were used for in vitro translation reactions ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue cultures that were cultivated on one filter membrane (3 cultures) were transferred as one sample into RNA Protection buffer (New England Biolabs) and RNA was isolated according to the manufacturer’s instructions (Monarch® Total RNA Miniprep Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated through SDS-PAGE and transferred to a PVDF membrane followed by incubation with anti-MBP monoclonal antibody (E8032S, NEB) and M2 Flag antibody (A8592 ...
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bioRxiv - Molecular Biology 2021Quote: ... The binding assay was performed by incubating these radiolabelled DNA fragments with RNA polymerase holoenzyme (NEB #MO551S) for 5min at 25 °C followed by loading in 4% polyacrylamide gel in 1X TBE buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The beads to which GFP-DSB-1 had been immobilized were subject to the phosphatase assay (NEB lambda phosphatase #P0753S ...
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bioRxiv - Microbiology 2022Quote: ... The SYBR Green assay was performed with the Luna Universal qPCR Master Mix (NEB, Ipswich, MA, USA) according to the manufacturer’s instructions with primers specific for HSV-1 gB (UL27 gene ...
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bioRxiv - Cell Biology 2022Quote: ... For CAK binding assays CDK2 was dephosphorylated by washing beads once in 1x λ-phosphatase buffer (NEB) supplied with 1 mM MnCl2 (NEB ...
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bioRxiv - Microbiology 2022Quote: ... For the noncapped assay the RNA in the reaction was treated with T4 PNK (NEB, Ipswich, MA) to produce a pool of 5’ monophosphate RNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... Real-time PCR was performed using SYBR green gene expression assays (New England Biolabs, catalog no. M3003S) on a QuantStudio 6 instrument (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: The dePARylation activity of PARG was measured in the PARP1 Histone H4 Activity Assay (#K611, Tulip BioLabs). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... and a subset of F0 embryos were dissociated and used in T7 endonuclease activity assays (NEB E3321) in order to validate successful CRISPR-Cas9 activity ...
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bioRxiv - Immunology 2024Quote: ... The qRT-PCR assays was performed using Luna Universal qPCR Master Mix (New England BioLabs, Frankfurt, Germany) on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories GmbH ...
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bioRxiv - Biochemistry 2024Quote: Cy3-labeled enhancer-promoter DNAs used for condensation assay were generated by PCR using Taq polymerase (NEB) with a Cy3-labeled primer (IDT ...
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bioRxiv - Genomics 2021Quote: ... using the Golden Gate assembly kit (NEB® Golden Gate Assembly Kit #E1601). Each guide sequence was cloned with its own U6 promoter and was followed by a sgRNA scaffold ...
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bioRxiv - Microbiology 2023Quote: ... A RNA cleanup kit (Monarch RNA Cleanup Kit T2050, New England Biolabs, France) was used to further clean the RNA samples ...
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bioRxiv - Synthetic Biology 2022Quote: ... plasmid (Monarch® PCR mini-prep kit) and gel extraction kits (Monarch® DNA gel extraction kit) were also obtained from NEB and used according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was incubated for 1 hour in the presence of an anti-SNAP-tag primary antibody (New England Biolabs #P9310S) diluted at 1:2000 in PBS + tween-20 0.1% ...
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bioRxiv - Biochemistry 2024Quote: ... whereas Benzonase-MBP was purified from outer membrane lysates obtained as with CXCL12 and isolated with use of amylose resin (NEB, E0821) as per manufacturer protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... on ice for 30 min and protein concentration measured by BCA assay (7780, New England Biolabs, Beverly, USA). Results were analyzed using the Compass software (ProteinSimple) ...
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bioRxiv - Microbiology 2021Quote: ... 20 µl of supernatant was mixed with 50 µl BioLux Gaussia Luciferase Assay substrate (New England Biolabs, Germany) and the luciferase activity was determined in a ClarioStar Plus plate reader (BMG Labtech ...
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bioRxiv - Molecular Biology 2022Quote: LAMP assays were optimized with Loxosceles similis DNA using Master Mix reagent (WarmStart® #M1800 – New England BioLabs). For this ...
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bioRxiv - Molecular Biology 2022Quote: ... end-point PCR assays were carried out with Hot Start Taq 2X Master Mix (New England Biolabs, USA) in a 20 μL reaction mixture containing 0.5 units of taq polymerase ...
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bioRxiv - Microbiology 2020Quote: All the templates required for the assay were labelled with [γ-32P] ATP using T4 DNA ligase (NEB) at room temperature for 1 h ...
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bioRxiv - Immunology 2020Quote: ... The nested PCR assays were run using the NEB enzyme Q5® Hot Start High-Fidelity (NEB, M0491) as follows ...
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bioRxiv - Biochemistry 2023Quote: ... RNA capping enzyme assays were performed in 10 μL of 1x RNA capping buffer (New England Biolabs, Inc.) containing 0.5 μM substrate RNA ...
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bioRxiv - Biochemistry 2024Quote: ... and RNA extraction for RT-qPCR assay using Luna® Universal One-Step RT-qPCR (New England Biolabs) and primers listed in table 4.
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bioRxiv - Genomics 2022Quote: ... and ribosomal RNAs were depleted using ribosomal RNA depletion kit (rRNA Depletion Kit, NEB). Ribosomal depleted RNA was used to make the RNAseq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid mini-prep kit and PCR DNA clean-up kits were provided by NEB.
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bioRxiv - Molecular Biology 2024Quote: ... purified using column DNA Clean-up kit (Monarch PCR & DNA Cleanup Kit, # T1030L, NEB) and mixed with Nhe1 and BamH1 double-digested and gel-purified target pCW57.1 vector in a molecular ratio of 1:2 ...
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bioRxiv - Developmental Biology 2021Quote: ... using HiScribe Sp6 kit (NEB) instead of T7.
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bioRxiv - Neuroscience 2021Quote: ... rRNA depletion kit (NEB #E6310), and Ampure XP beads (Beckman #A63881) ...