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Citations for New England Biolabs :
1 - 50 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Bacterial artificial chromosome (BAC) sequences from pBELOBAC11 (NEB) were amplified using forward (5’-TTAATTAAcgtcgaccaattctcatg ...
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bioRxiv - Evolutionary Biology 2020Quote: ... six parallel PCR reactions (Q5 DNA polymerase, NEB) were set up with 30 µl of sample ...
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bioRxiv - Cancer Biology 2024Quote: ... and library prep were performed in parallel using the Ultra II Directional RNA-Seq Kit (NEB E7760) and reads were sequenced 2x50bp on an Illumina NovaSeq S4.
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bioRxiv - Cell Biology 2023Quote: ... and libraries for massive parallel sequencing were constructed with NEBNext® UltraTM RNA Library Prep Kit for Illumina (NEB). Library quality was assessed with Agilent Bioanalyzer 2100 system ...
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bioRxiv - Microbiology 2021Quote: ... A parallel reaction was prepared following the manufacturer’s protocol (New England Biolabs) and using 2 mM SAM to obtain m7G*ppp-RNA ...
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bioRxiv - Developmental Biology 2023Quote: ... building parallel total-RNA and poly(A)+ Illumina strand-specific libraries using the Ultra II RNA-seq library kit (NEB #E7765) (see below) ...
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bioRxiv - Genetics 2021Quote: ... 2 × 2 µg of gDNA were digested in parallel with 50 units of DpnII (NEB #R0543L) and NlaIII (NEB #R0125L ...
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bioRxiv - Cell Biology 2023Quote: ... The Oxiselect Comet Assay Kit (Cell-Biolabs) was used as a reference for the experiment ...
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bioRxiv - Molecular Biology 2023Quote: ... Artificial mutations were introduced in the minigene by Reverse Site-Directed Mutagenesis with Phusion High-Fidelity DNA Polymerase (NEB), followed by 5’ phosphorylation with T4 Polynucleotide Kinase (NEB ...
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bioRxiv - Genomics 2021Quote: ... The PCR fragments were recombined to the vector in 50 parallel NEBuilder HiFi DNA assembly reactions (NEB) according to the manufacturer’s instructions using 150 ng of linearized vector and 50 ng of custom CpG-free adapter-ligated genomic DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Mutations and deletions were introduced by site-directed PCR mutagenesis (TRF1, TRF2, BLM) or during artificial DNA synthesis (Top3A) and cloned by the recombination-based HiFi assembly strategy (NEB). Amino acid positions in each protein correspond to the indicated reference sequences.
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bioRxiv - Immunology 2021Quote: Purified gDNA samples were diluted 10-fold and in parallel into Cutsmart-buffered BamHI-HF (New England Biolabs) restriction digestions in the presence of 0.1% Pluronic F-68 (50μL final volume ...
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bioRxiv - Microbiology 2020Quote: ... the BioLux® Gaussia Luciferase Assay Kit (NEB, discontinued) or the GAR-2B Gaussia Luciferase Assay (Targeting Systems ...
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bioRxiv - Genomics 2023Quote: ... each were incubated in parallel with 100 U or 0 U of M.CviPI (Xu et al. 1998) (New England Biolabs) in methylation buffer (320 µM S-adenosyl-L-methionine in CRB ...
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bioRxiv - Genomics 2023Quote: ... the purified amplicons were divided into parallel reactions (about 5ug each) and were digested with Lambda Exonuclease (0.5U/ul) in 1x buffer (NEB M0262L) at 37 °C for 2 hours and purified using Zymo ssDNA/RNA clean & concentrator kit following manufacturer’s instructions (Zymo D7011) ...
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bioRxiv - Cell Biology 2020Quote: ... or BioLux® Cypridina Luciferase Assay Kit (New England Biolabs) and Gaussia luciferase was assayed from 1:40 diluted cell culture supernatant using either the Stop & Glo reagent from the DualLuciferase® Reporter Assay System (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... or BioLux® Gaussia Luciferase Assay Kit (New England Biolabs) in a Lumat LB 9507 luminometer (Berthold Technologies).
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bioRxiv - Microbiology 2021Quote: ... or a Biolux Gaussia luciferase assay kit (New England BioLabs) and a GloMax microplate reader (Promega) ...
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bioRxiv - Genomics 2023Quote: ... the purified amplicons were divided into parallel reactions (about 5ug each) and were digested with DpnII (1U/ul) in 1x NEBuffer DpnII (NEB R0543L) at 37 °C for 3 hours and purified with QIAquick PCR purification kit ...
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bioRxiv - Microbiology 2024Quote: ... and Biolux Cypridina luciferase assay kit (New England Biolabs, USA; #E3309), respectively ...
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bioRxiv - Microbiology 2024Quote: In vitro transcription/translation assays were conducted using the PURExpress kit (NEB) according to the manufacturer’s protocol with a 2-hour incubation at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: Coupled transcription-translation assays were carried out with the PURExpress kit (New England BioLabs) as described in (Lukasiewicz and Contreras ...
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bioRxiv - Biochemistry 2023Quote: ... Assays were set-up using PURExpress in vitro protein synthesis kit (New England Biolabs) with a cloned full-length DHFR template ...
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bioRxiv - Molecular Biology 2022Quote: RNA was purified by silica-based SPE using Monarch RNA Cleanup Kits (for in vitro assays) or Total RNA Miniprep Kit for intracellular samples (NEB).
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bioRxiv - Immunology 2021Quote: We quantified plasma luciferase activity by a BioLux Gaussia Luciferase Assay Kit (NEB, Ipswich, MA) from 10 μL plasma acquired by retro-orbital bleeding ...
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bioRxiv - Molecular Biology 2023Quote: ... using the High Sensitivity DNA Assay and NEBNext Library Quant Kit for Illumina (NEB, E7630). Finally ...
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bioRxiv - Microbiology 2024Quote: ... The in vitro translation assay was performed using PURExpress In Vitro Protein Synthesis Kit (NEB). 24 μM of antibiotics were added individually to the translation mixture and incubated at 37 °C for 4 hours ...
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bioRxiv - Bioengineering 2024Quote: In vitro coupled transcription-translation assays were performed using the PURExpress Kit (New England Biolabs). Template plasmids utilized a uxuB-sfGFP fusion sequence ...
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bioRxiv - Synthetic Biology 2020Quote: ... GLuc activity in samples was measured using the Biolux Gaussia Luciferase Assay kit (NEB, Ipswich, MA). GLuc assay solution (50 μL ...
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bioRxiv - Cell Biology 2020Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was performed using the Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB) with the CDC-derived primers for N1 and N2 gene targets and the reaction was performed using the QuantStudio™ 5 System (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: The in vitro transcription assay was conducted using the HiScribe T7 High Yield RNA Synthesis Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... cell-free transcription-translation assays were carried out using the PURExpress® In Vitro Protein Synthesis Kit (NEB) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... while Gluc and Cluc expression levels were determined using Biolux Gaussia or Cypridina luciferase assay kits (New England BioLabs) and a microplate reader ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 pmol of TP_MAAAPQKCAAA* mRNA (see “Toeprinting assays” section) and components of the PURExpress ΔRibosomes Kit (New England Biolabs). The reaction was incubated at 37ºC for 20 min and then diluted in 50 mM Hepes KOH pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... and blotted to a membrane followed by hybridization with radioactive probe [62] prepared using the random primers labelingNEBlot® kit (New England Biolabs, USA). The band detection was carried out using Typhoon® phosphor imager (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... Indel frequencies at the SaCas9 target site were assessed via a T7E1 assay with the EnGen Mutation Detection Kit (NEB), using the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Final Illumina libraries were assessed for quality using an Agilent Bioanalyzer DNA High Sensitivity Assay and qPCR quantification was performed using NEBNext Library Quant kit for Illumina (New England Biolabs). Individual libraries were pooled equimolarly ...
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bioRxiv - Microbiology 2021Quote: ... was utilized using SARS-CoV-2 specific primers[43] or SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs), which became available in assays after September 15 ...
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bioRxiv - Molecular Biology 2022Quote: ... Concentrations were measured by Qubit RNA HS assay kit and 750 ng of Dnase-treated RNA was used for rRNA depletion by NEB Next rRNA depletion kit (Human/Mouse/Rat) ...
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bioRxiv - Plant Biology 2021Quote: ... All other mutations of the constructs used for in vitro and in vivo assays were introduced using a Q5 site-directed mutagenesis kit (NEB). All constructs were verified by DNA sequencing.
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bioRxiv - Biochemistry 2023Quote: A luminescence based translation inhibition assay was performed using an in-vitro translation PURExpress® Δ Ribosome Kit from (NEB) The constituents of the kit were incubated with 25 ng pMSR DNA template ...
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bioRxiv - Cancer Biology 2024Quote: ... indel assay was performed by T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321) to detect how efficiently a given sgRNA produces indels at targeted genomic DNA locus ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then washed and assayed for expression of Gaussian Luciferase in cell lysates using a BioLux Gaussia Luciferase Assay Kit (New England BioLabs) as per the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
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bioRxiv - Genetics 2022Quote: ... using a T7E1 assay (NEB), and compared to the activity of Tet2 sgRNA that has been shown to modify the mouse genome efficiently95 ...
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bioRxiv - Developmental Biology 2024Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
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bioRxiv - Microbiology 2021Quote: ... 3 µl of extracted RNA was used in a TaqMan one-step qRT/PCR assay (Luna® Universal One-Step RT-qPCR Kit, NEB). TaqMan analysis was carried out with primer/probe combination described in Table 1 and analysis performed on the QuantStudio™ 6 Flex System (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2020Quote: In vitro trans-translation assays were performed using the PURExpress In Vitro Protein Synthesis and Δ Ribosome kits (New England Biolabs). For trans-translation assays ...