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Citations for New England Biolabs :
51 - 100 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2020Quote: The 16S rRNA and gltA amplicons from PCR assays were cleaned with the ExoSAP-IT kit (E1050, New England Biolabs, US) and Sanger sequenced ...
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bioRxiv - Cell Biology 2020Quote: ... 200 ng/μL was used for subsequent reverse transcription assay with Luna® Universal One Step RT-qPCR kit (E3005, New England Biolabs) according manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The mutant and wild-type target RNA were subsequently amplified using either the NEB Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB E3019), Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB M3019 ...
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bioRxiv - Microbiology 2024Quote: ... Gaussia luciferase and Cypridina luciferase expression levels were measured with a Biolux Gaussia luciferase assay kit (New England Biolabs, USA; #E3300) and Biolux Cypridina luciferase assay kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... The qRT-PCR assay was performed using 500 ng of total RNA and the Luna Universal One-Step RT-qPCR Kit (NEB, E3005S) on a QuantStudio 3 system (Applied Biosystems) ...
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bioRxiv - Bioengineering 2024Quote: The colorimetric assay was performed according to the instructions in the NEB WarmStart Colorimetric LAMP 2X Master Mix Kit containing UDG (NEB M1804). This mix provides a distinctive red-to-yellow transition in the presence of a pH indicator dye to indicate a positive result ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to nitrocellulose membranes (Biolabs, 1620115). Membranes were then incubated with primary antibody diluted in 5% milk or 2.5% BSA in Triton X-100-TBS buffer (T-TBS ...
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bioRxiv - Genomics 2020Quote: ... a dsDNA HS qubit assay and also by a NEBNext library quantitation Assay (New England Biolabs) before sequencing on Illumina’s HiSeq2500v4
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bioRxiv - Developmental Biology 2021Quote: ... The T7E1 assay (Cat#M0302, NEB) was then applied as in (Sato et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Mutants selected for cloning were also analysed via T7 endonuclease I-based (T7E1) heteroduplex assay according to the manufacturers protocol (EnGen® Mutation Detection Kit, NEB #E3321), and the length of digested and undigested fragments were visually compared by gel electrophoresis (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... as well as wildtype (WT) N2aC were assayed for mis-matched DNA pairs via T7 endonuclease assay (EnGen Mutation Detection Kit; New England Biolabs, Ipswich, MA) with PCR primers targeted towards our genomic region of interest (Integrated DNA technologies ...
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bioRxiv - Synthetic Biology 2022Quote: ... 200 fmol of DNA (determined following quantification with the Qubit™ dsDNA HS Assay Kit) was processed using the NEBNext Companion Module for ONT Ligation Sequencing (NEB, #E7180). Sequencing adapters were added using the Ligation Sequencing Kit (ONT ...
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bioRxiv - Cancer Biology 2022Quote: ... We designed spCas9 guide RNAs to target codon 12 of the human KRAS gene and selected the one with highest targeting efficiency determined by T7E1 mismatch assay (EnGen Mutation Detection Kit, New England Biolabs, Ipswich, MA). Truncated guide RNA was produced by PCR assembly of a guide RNA template (56 ...
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bioRxiv - Immunology 2024Quote: ... fragmented DNA obtained from the CUT and RUN assay was processed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, Cat# E7645L), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... fragmented DNA obtained from the CUT and RUN assay was processed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, Cat# E7645L), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... MBP pulldown assays were performed similar to GST pull down assays using Amylose resin (New England Biolabs) and 1 nmol of MBP-Oskar 292-325 and 5 nmol of Smaug 37-281.
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Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: ... Lucia and Cypridina luciferase activity was quantified by bioluminescence from aliquots of the cell supernatant (BioLux Cypridina Luciferase assay kit, New England Biolabs; Quanti-Luc, InvivoGen). Reference plasmid-normalized luciferase activity was from the average of three independent transfections ...
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bioRxiv - Genetics 2022Quote: ... T7 Endonuclease I assay (T7EI; NEB M0302L) or restriction enzyme digestion using Mse I (NEB R0525L) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used the T7E1 assay (NEB #E3321) to determine potential mutations for every single fly following the protocol in (Zhu et al. ...
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bioRxiv - Cell Biology 2023Quote: ... a T7 endonuclease I cutting assay (NEB) was used to identify the extent of insertions/deletions for each guide ...
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bioRxiv - Genomics 2024Quote: ... for mouse assay and HaeIII (NEB, R0108L) for human assay] ...
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bioRxiv - Immunology 2024Quote: ... RNA was purified with silica membrane spin columns (NEB, T2050) and quantified by NanoDrop ...
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bioRxiv - Microbiology 2024Quote: ... and the PNGase F assay (New England Biolabs) was completed following the manufacturer’s instructions using 15 µg of protein ...
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bioRxiv - Molecular Biology 2023Quote: ... coli transposition assays were treated with AvrII (NEB) to reduce the proportion of contaminating pDonor ...
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bioRxiv - Developmental Biology 2024Quote: ... using the Gibson assembly assay (NEB Cat#E2611S). Plasmid was injected at the one cell stage ...
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bioRxiv - Genetics 2021Quote: ... followed by T7 Endonuclease I Assay (New England Biolabs). 30 PCR cycles were run in all samples ...
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bioRxiv - Neuroscience 2020Quote: ... we performed a T7 endonuclease I (T7EI) assay (NEB) on approximately 10 F1 embryos from a clutch to confirm whether any mutations had occurred in the germline of the injected parent ...
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bioRxiv - Neuroscience 2023Quote: ... used in T7 endonuclease I activity assays (NEB E3321) as previously described (Baker et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... restriction enzyme digest assay with BtsCI (New England Biolabs) was performed to identify WT ...
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bioRxiv - Cancer Biology 2023Quote: ... along with the T7 Endonuclease I (T7EI) assay (NEB).
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bioRxiv - Genetics 2023Quote: ... followed by T7 endonuclease I assay (New England Biolabs) assay to detect mutations ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by T7 endonuclease I assay (New England Biolabs) to detect indel mutations ...
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bioRxiv - Developmental Biology 2024Quote: ... A T7 endonuclease I assay (New England Biolabs M0302S) was performed to quantify indel frequency of gRNA pairs following PCR amplification (Phusion Hot Start Flex DNA Polymerase ...
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bioRxiv - Cancer Biology 2021Quote: ... The membrane was incubated with HRP-conjugated secondary antibodies (1:5000, NEB) for 45 mins at RT ...
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bioRxiv - Physiology 2022Quote: ... Wet transferred nitrocellulose membranes were incubated in anti-crotonyl-lysine (PTM Biolabs), anti-acetyl-lysine (PTM Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated with anti-mouse HRP antibody (1:5000, NEB 7076S) for 1h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and the membrane was suspended in a buffer with proteinase K (NEB). RNA isolation was performed with phenol/chloroform extraction and purified on spin columns (Zymo Research) ...
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bioRxiv - Genetics 2021Quote: ... T7 endonuclease 1 assay was performed following manufacturer’s protocol (NEB).
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bioRxiv - Developmental Biology 2022Quote: ... 5’-CTTTTGACATCCGCTTCTGC-3’ followed by a T7 endonuclease assay (NEB) to detect indels ...
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bioRxiv - Developmental Biology 2020Quote: ... Kinase assays were performed using recombinant murine c-Abl (NEB). The amounts of GST-fusion protein to add to the reaction were calibrated using western blots of the purification products with mouse anti-NICD (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... All assays were done in Escherichia coli (strain: NEB Turbo) following the same plasmid protection assay described previously ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were analysed by an MSD Elisa assay (Pacific Biolabs) using a CEP55 antibody (Novux ...
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bioRxiv - Developmental Biology 2020Quote: ... Mutations were detected through a T7 endonuclease I assay (NEB)(76) ...
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bioRxiv - Bioengineering 2024Quote: DNA cutting assays were performed following New England Biolabs (NEB) method.39,40 Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... The assays are composed of 1X Isothermal Buffer (NEB #B0537), 6 mM Magnesium Sulfate (MgSO4 – NEB #B1003) ...
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bioRxiv - Molecular Biology 2024Quote: ... was confirmed using a Lambda phosphatase assay (New England BioLabs).
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bioRxiv - Microbiology 2024Quote: Assays were performed using the PURExpress ΔRibosome (New England Biolabs) according to manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... Nitrocellulose membrane-transferred proteins were incubated with anti-PfGet3 or anti-MBP (NEB), probed with corresponding HRP-conjugated secondary antibodies and developed by chemiluminescence.
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bioRxiv - Microbiology 2021Quote: ... The membrane was probed with horseradish peroxidase (HRP)-conjugated anti-MBP antibody (NEB) and HRP-conjugated anti-FLAG antibody to detect MBP-TcpB and FBXO22 ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were probed with rabbit polyclonal anti-pansuccinyllysine antibody (PTM Biolabs, PTM-401) at a dilution of 1:1000 ...