Labshake search
Citations for New England Biolabs :
401 - 450 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Monarch® PCR & DNA Cleanup Kit (NEB), or Gene Jet gel extraction kit (Thermo Scientific) ...
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bioRxiv - Genomics 2024Quote: Strand cDNA Synthesis Kit (New England Biolabs) according to manufacturers recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... Monarch PCR purification kit (Biolabs, New England) used to purify the resulting amplified cDNA constructs ...
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bioRxiv - Cell Biology 2024Quote: ... the Q5 Site-Directed Mutagenesis Kit (NEB) was used to delete the miniTurbo sequence from the above pLVX-KRT5-miniTurbo-FLAG-IRES-Puro and pLVX-KRT6A-miniTurbo-FLAG-IRES-Puro constructs ...
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bioRxiv - Developmental Biology 2024Quote: ... The NEBuilder HiFi DNA Assembly Kit (NEB) was used for the construction of chimeric Pk1 and Pk2 mutants ...
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bioRxiv - Cell Biology 2024Quote: ... and Monarch RNA Clean Up Kit (NEB). Triplicated samples were pooled prior to sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... the Q5 Site-Directed Mutagenesis Kit (NEB). The Gibson assembly was performed using the NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... with NEBNext Bacterial rRNA depletion Kit (NEB) and 150 bp paired-end sequencing was performed on a NovaSeq 6000 (Illumina) ...
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bioRxiv - Immunology 2024Quote: ... and the NEBNext RNA Ultra kit (NEB). Libraries were evenly split across 2 lanes of an Illumina Hiseq flow cell and sequenced (150 bp paired-end reads ...
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bioRxiv - Genetics 2024Quote: ... followed by LUNA Universal qPCR kit (NEB). RT-qPCR was performed in a Bio-Rad CFX Opus 96 Real-Time PCR System ...
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bioRxiv - Genetics 2024Quote: ... followed by LUNA Universal qPCR kit (NEB). RT-qPCR was performed in a Bio-Rad CFX Opus 96 Real-Time PCR System ...
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bioRxiv - Microbiology 2024Quote: ... or a Monarch gDNA extraction kit (NEB). Bacterial genomic sequencing was performed by either MinION Mk1C nanopore sequencing or PacBio sequencing.
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bioRxiv - Synthetic Biology 2024Quote: ... or Monarch® Plasmid Miniprep Kit (NEB). When purifying plasmid DNA from C ...
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bioRxiv - Systems Biology 2024Quote: ... HiScribe® SP6 RNA Synthesis Kit (NEB) was used to in-vitro transcribe a library of mRNA reporters from linearized plasmids ...
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bioRxiv - Molecular Biology 2024Quote: ... using NEBuilder HiFi DNA assembly kit (NEB). pBAD-His6-Ocr was obtained by cloning 0.3 gene from T7 phage into pBAD-HisB43 vector using Gibson Assembly Master Mix (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... Q5 Site-Directed Mutagenesis Kit (E0554S, NEB), Recombinant protein G sepharose beads (101242 ...
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bioRxiv - Plant Biology 2024Quote: ... NEBNext Enzymatic Methyl-seq Kit (NEB, E7120L) and Multiplex Oligos for Enzymatic Methyl-seq (E7140S ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid using the HiFi Assembly kit (NEB) to produce an N-terminal six histidine-tagged SUMO- NSP13 fusion cassette (6XHis-SUMO-NSP13) ...
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bioRxiv - Bioengineering 2024Quote: The Monarch Total RNA Miniprep Kit (NEB) was used for RNA purification from cell lysates ...
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bioRxiv - Cell Biology 2024Quote: For digest with PNGase (kit, NEB P0704S), 10x glycoprotein denaturation buffer was diluted to 1x with water and then 80 µl of this added to the bead pellet ...
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bioRxiv - Evolutionary Biology 2021Quote: DNA libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit kit by New England Biolabs (NEB) and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
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bioRxiv - Genomics 2020Quote: NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext rRNA Depletion Kit (NEB, Ipswich, MA) was used to generate RNA-seq libraries from purified RNA following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Libraries were constructed using NEBNext Ultra RNA Library Prep for Illumina kits (New England Biolabs kits E7490 and E7530), as per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... a ChIP-seq library was made using the NEBNext Ultra II DNA Library Prep Kit for Illumina kit (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... libraries were quantified by qPCR using a library quantification kit (NEBNext® Library Quantification Kit, E7630, New England Biolabs). Illumina sequencing was performed on a HiSeq2500 instrument for 100 single end read cycles (Genome Sequencing Core ...
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bioRxiv - Physiology 2023Quote: ... including DNase 1 treatment (Monarch Total RNA Miniprep kit, #T2010, New England Biolabs, USA and QIAGEN RNeasyMini kit, #74104). Purity and quantity of RNA were assessed by determining the optical density (OD ...
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bioRxiv - Genomics 2023Quote: ... Final immunoprecipitated DNA samples from the kit were then purified using a Monarch PCR and DNA cleanup kit (5μg) (NEB), with a final elution volume of 50μL in TE buffer ...
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bioRxiv - Cell Biology 2023Quote: The novel entry modules created for this study were cloned by insertion of PCR-amplified sequences in pJet1.3 or pMiniT 2.0 following manufacturer’s instructions (CloneJET PCR Cloning Kit, Thermo Scientific; NEB® PCR Cloning Kit, NEB). BsaI recognition sites followed by module-specific overhangs were added to each primer used to amplify entry sequences ...
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bioRxiv - Cell Biology 2024Quote: ... All mGreenLantern-tagged constructs consist of ORFs inserted into the multiple cloning site of pcDNA3.1-mGreenLantern using a Gibson cloning kit (NEBuilder HiFi DNA Assembly Cloning Kit, New England Biolabs). Intersectin(SH35 ...
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bioRxiv - Microbiology 2021Quote: ... the DNA products from each ChIP assay were first end repaired with end repair enzyme mix (New England Biolabs, Inc., Cat#M6630), then ligated to NEBNext adaptor included in the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (Cat#E7645L ...
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bioRxiv - Microbiology 2022Quote: ... immitis and Wolbachia qPCR assays were carried out in 10 μL final volumes of Luna® Universal Probe qPCR Master Mix (New England Biolabs, Australia) containing 1 μL of template DNA isolated from whole dog blood and primers and probe at a final concentration of 400 nM and 100 nM ...
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bioRxiv - Microbiology 2024Quote: A number of plasmids encoding wild-type TpiA or its derived pentapeptide-inserted variants were selected from the opening library and separately used as template for PURExpress® In Vitro Protein Synthesis assays (New England BioLabs inc.) according to the supplier instructions ...
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bioRxiv - Microbiology 2024Quote: ... All qPCR assays were carried out in 20 μL reaction mixtures using 2X Luna Universal Probe Mastermix (New England Biolabs, MA, USA), 2.5 μL extracted genomic DNA (unless otherwise stated) ...
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bioRxiv - Developmental Biology 2021Quote: ... the RNA samples isolated by PureLink Mini kit were subsequently purified by Magnetic mRNA Isolation kit (New England Biolabs, USA). mRNA concentration was estimated by Qubit RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... amplicons were gel purified with the InnuPREP Gel Extraction Kit (Analytik Jena) or the Monarch DNA Gel Extraction Kit (NEB), and submitted for Sanger sequencing (Eurofins Genomics) ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted using the Qiagen RNeasy Micro kit and libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs). All libraries were sequenced on an Illumina HiSeq 2500 with 100 bp single-end reads.
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bioRxiv - Molecular Biology 2021Quote: ... Adaptor and index primers were added to the sheared DNA fragments using a next-generation sequencing library preparation kit (NEBNext Ultra DNA Library Prep Kit for Illumina; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted using illustra RNAspin Mini isolation kit (Cytiva) or Monarch Total RNA Miniprep Kit (New England Biolabs) subsequent to spheroblast construction using 10 U of Zymolyase (ZYMO Research ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples with RIN value higher than 8 were processed using the NEBNext poly(A) mRNA Magnetic Isolation kit and the NEBNext Ultra II RNA Library Prep kit for Illumina (New England Biolabs), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR/reaction cleanup and gel extraction were conducted with the Monarch PCR and DNA cleanup kit and Monarch DNA gel extraction kits (NEB). E ...
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bioRxiv - Microbiology 2021Quote: ... cholerae and phages was extracted using commercially available kits (Qiagen DNeasy Blood and Tissue or Monarch genomic DNA purification kit (New England BioLabs). For preparation of phage DNA ...
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bioRxiv - Microbiology 2022Quote: ... After enrichment of mRNA using the Universal Ribodepletion Kit cDNA preparation was performed with the Next Ultra Directional Library Preparation Kit for Illumina (NEB). The cDNA was sequenced on HiSeq 3000 (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... 60 ng of total RNA was used for library preparation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext rRNA Depletion Kit (New England Biolabs). The libraries were paired-end sequenced (2 × 75 bp ...
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All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cellsbioRxiv - Neuroscience 2021Quote: ... and RNA was consecutively isolated using a column based RNA isolation kit according to the manufacturer’s instructions (Monarch® Total RNA Miniprep Kit; #T2010S New England Biolabs). Strand specific cDNA library preparation from polyA enriched RNA (150 bp mean read length ...
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bioRxiv - Systems Biology 2021Quote: Bulk RNA-seq libraries were prepared from 1000 ng of RNA using the NEBNext Ultra RNA Library Prep kit for Illumina or the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enrichment of mRNA was done using the Universal Ribodepletion Kit followed by Next Ultra Directional Library Preparation Kit for Illumina (NEB). The cDNA was sequenced on HiSeq™ 2500 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Libraries were constructed from 1 µg of total RNA using Illumina’s TruSeq stranded mRNA Library Preparation Kit or from 500 ng total RNA using NEBNext Ultra II RNA -polyA+ (mRNA) Library Preparation Kit (New England Biolabs) following the manufacturer’s description.
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bioRxiv - Genetics 2021Quote: ... RNA samples were depleted of rRNA using the QIAseq FastSelect RNA Removal Kit then processed into libraries using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Samples were recovered by phenol/CHCl3/isoamyl alcohol extraction twice and purified as per kit instruction using Monarch PCR and DNA cleanup Kit (New England BioLabs). DNA was suspended in 200 ul pre-warm (55°C ...
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bioRxiv - Developmental Biology 2021Quote: ... or deletions in full-length Gpr161 were generated using Quikchange site-directed mutagenesis kit (Stratagene or Q5 Mutagenesis Kit (NEB).