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Citations for New England Biolabs :
101 - 150 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... cell viability was determined utilizing an XTT assay (New England Biolabs). Similar results were obtained utilizing calcium phosphate transfection methods ...
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bioRxiv - Microbiology 2020Quote: ... we used a colorimetric assay using p-Nitrophenyl phosphate (pNPP) (NEB) as a substrate ...
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bioRxiv - Synthetic Biology 2022Quote: ... To perform the qPCR assay 0.95 µL qPCR mastermix (M3003, NEB) containing 2 ng/µL template DNA was dispensed using a nanoliter dispenser (Cobra 4-channel dispenser ...
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bioRxiv - Molecular Biology 2023Quote: ... The optimized assay reactions comprised 1x CutSmart buffer (New England BioLabs) (20 mM Tris-acetate pH 7.9 ...
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bioRxiv - Cell Biology 2023Quote: ... cell viability was determined utilizing an XTT assay (New England Biolabs). 10 µL of unfractionated conditioned media or 5 µL of the 2K ...
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bioRxiv - Microbiology 2022Quote: ... and subsequent restriction enzyme assay of exon 5 (with BSaaI, NEB) and exon 7 (BSErI ...
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bioRxiv - Neuroscience 2023Quote: The assay was carried out following the manufacturer’s instructions (NEB, P0753S). Details are given in SI Appendix.
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bioRxiv - Developmental Biology 2023Quote: ... We then performed a T7E1 nuclease assay (M0302, New England Biolabs) according to the manufacturer’s protocol and resolved digested products on a 1.2% agarose gel containing STBYTM safe DNA gel stain (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: T7EI assay was performed according to the manufacturer’s instructions (NEB M0302L). Briefly ...
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bioRxiv - Biochemistry 2024Quote: All phage plaque assays were carried out in E.coli DH10B (NEB) using the double layer top agar method ...
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bioRxiv - Neuroscience 2024Quote: ... The assay was initiated by adding TEV protease (New England Biolabs) to cleave the MBP tag from the TDP-43-MBP constructs ...
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bioRxiv - Cell Biology 2022Quote: ... and Calnexin (membrane) using qPCR primers (IDT) with Q5 polymerase (New England Biolabs, M0492) was measured with a highly sensitive dsDNA detection dye (Lumiprobe ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... The membrane was incubated with HRP-conjugated (1/2,500 New England Biolabs, Ipswich, MA) or IRDye® (1/10,000 LI-COR Biosciences ...
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bioRxiv - Biophysics 2024Quote: ... The supernatants (membrane extracts) were collected and mixed with amylose resin (New England Biolabs). After 2 hrs of incubation at 4 °C ...
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bioRxiv - Bioengineering 2020Quote: To prepare the DNA template for curtain assays λDNA (125 μg, NEB) was incubated with 2 μΜ biotinylated oligo complementary to one of the two 12 nt cohesive ends in λDNA in T4 DNA ligase reaction buffer (NEB ...
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bioRxiv - Microbiology 2021Quote: All plasmid protection assays were done in Escherichia coli (strain: NEB Turbo). As described previously12 ...
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bioRxiv - Molecular Biology 2023Quote: ... 400ng of gDNA was used for T7 endonuclease assay (NEB Cat#M0302). Assays were run in TBE polyacrylamide gels and visualized using Gel Red.
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bioRxiv - Synthetic Biology 2024Quote: ... PCR assays were performed using the Luna Universal qPCR Master Mix (NEB) in a MasterCycler RealPlex 4 (Eppendorf) ...
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bioRxiv - Cell Biology 2023Quote: Amylose pulldown assay was performed with Amylose Sepharose beads (New England BioLabs) in a pulldown buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Endo H assay was performed following manufacturer’s instructions (New England Biolabs, P0702S). Following addition of Glycoprotein Denaturing Buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were hybridized with primary and HRP coupled secondary antibodies (New England Biolabs, Ipswich, MA). Membranes were revealed by chemiluminescence with SuperSignal West Dura or Femto reagents and data acquired using the G-BOX-iChemi Chemiluminescence Image Capture system (Syngene ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was hybridised with radioactively labelled probes (T4 PNK, M0201, New England Biolabs (NEB)) in Ultrahyb buffer at 35 ᵒC and exposed to a phosphor screen that was scanned using Typhoon scanner (Cytiva).
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bioRxiv - Plant Biology 2023Quote: ... The membrane was hybridised with radioactively labelled probes (T4 PNK, M0201, New England Biolabs (NEB)) in Ultrahyb buffer at 35 ᵒC and exposed to a phosphor screen that was scanned using Typhoon scanner (Cytiva).
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bioRxiv - Biochemistry 2024Quote: ... the membrane was incubated with HRP-conjugated secondary antibody (goat anti-rabbit HRP, BioLabs 7074P2) in 3% BSA in PBS-T shaking for 1-2 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was then probed with the primary anti-MBP antibody (NEB E8032S, 1:10000) diluted in 5% NFDM (overnight ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR assays were conducted with Luna Universal qPCR Master Mix (New England Biolabs). Primers for qRT-PCR analyses are listed in Table S4 ...
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bioRxiv - Cell Biology 2020Quote: All in vitro kinase assays were done in NEBuffer for protein kinase (NEB) at 30°C ...
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bioRxiv - Microbiology 2022Quote: In vitro Cas12a cleavage assays were performed using EnGen Lba Cas12a (NEB #M0653T) and crRNAs that were synthesized commercially (IDT ...
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bioRxiv - Molecular Biology 2020Quote: ... The beads used in this assay were as followed: Streptavidin Magnetic Beads (NEB), EZview(TM ...
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bioRxiv - Genetics 2020Quote: ... PCP products were used as template for T7 endonuclease assay (New England BioLabs), following manufacturer instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The qRT-PCR assay was performed using Luna Universal qPCR Master Mix (NEB) with a total volume of 20 µl ...
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bioRxiv - Developmental Biology 2024Quote: ... and subjected to T7E1 nuclease assay according to the manufacturer’s instructions (NEB, M0302L). Digested products were resolved on a 1-2% agarose gel containing SYBR safe (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... Transfection efficiency was assessed by a T7 assay three days post-transfection (NEB EnGen® Mutation Detection Kit ...
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bioRxiv - Microbiology 2024Quote: A colorimetric assay using the substrate p-Nitrophenyl phosphate (PNPP) (New England Biolabs) was used to quantify alkaline phosphatase activity in colonies grown on MOPS agar plates with no added BCIP using the inoculation regime described above ...
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bioRxiv - Molecular Biology 2024Quote: ... then labelled with 2 μM of cell-membrane impermeable dyes (CLIP-surface 547 (BC-DY547, NEB) for FLAG-CLIP tagged plasmids ...
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bioRxiv - Cell Biology 2020Quote: ... a P2A self-cleavage site and a CAAX membrane localization tag were added using Q5 mutagenesis (NEB) with the following primers ...
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bioRxiv - Immunology 2020Quote: ... All in vitro activity assays with recombinant GST-IdeZ or IdeZ (NEB Catalog #P0770S) (1ug per reaction ...
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bioRxiv - Molecular Biology 2022Quote: DNA substrates for gel shift assays were amplified by PCR using Q5 Polymerase (NEB) and purified using a DNA Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Biophysics 2021Quote: ... The reconstitusion was confirmed by MNase assays in which MNases (M0247S, New England BioLabs) digest linker DNA for 30 minutes and the digested products were run on 1% agarose gel ...
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bioRxiv - Immunology 2020Quote: ... The PCR assays using NEB enzyme Q5® Hot Start High-Fidelity (NEB, M0491) with the following thermocycling conditions ...
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bioRxiv - Microbiology 2023Quote: ... LucEV-A71 assay: The plasmid was linearised using Mlu1 restriction enzyme (NEB, Cat # R3198S), and purified using Phenol:Chloroform:Isoamyl Alcohol (PCI 24:25:1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein amounts for deadenylation assays were estimated against BSA standards (New England Biolabs, B9000S).
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bioRxiv - Cancer Biology 2023Quote: ... and regularly tested for mycoplasma contamination (PCR-based assay from Minerva-Biolabs, Berlin, Germany).
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bioRxiv - Molecular Biology 2024Quote: For lambda phosphatase assay the reactive from Lambda Protein Phosphatase (Lambda PP) (P0753L, NEB) were used and followed the protocol from the manufacturer.
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bioRxiv - Genomics 2022Quote: Ribosomal RNA depletion kit (rRNA Depletion Kit, E6310L, NEB)
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bioRxiv - Biochemistry 2023Quote: ... first with 20 pmol membrane impermeable SNAP dye (10 μM SNAP-Surface® 488, New England BioLabs Inc.) followed by 20 pmol membrane permeable SNAP dye (10 μM SNAP-Cell® 647-SiR ...
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bioRxiv - Genetics 2021Quote: ... by PCR amplification of target locus followed by T7 Endonuclease I assay (New England Biolabs).
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bioRxiv - Bioengineering 2020Quote: The following components comprised the RT-LAMP assay: 4 mM of MgSO4 (New England Biolabs), 1× final concentration of the isothermal amplification buffer (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... The assay mixture was then analyzed using western blotting by anti-MBP (New England Biolabs) and anti-His (D2951 ...
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bioRxiv - Immunology 2021Quote: ... Enzymatic assays for PNGase F and Endo S were performed according to manufacturer’s instructions (NEB). The effect of the enzymatic assays was either analyzed by Coomassie staining or immunoblotting.