Labshake search
Citations for New England Biolabs :
451 - 500 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... RNA-sequencing libraries were prepared with a NEBNext rRNA Depletion Kit and an ENBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). Then ...
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bioRxiv - Plant Biology 2023Quote: ... from 100 to 1000 ng of total RNA using a stranded kit (Illumina TruSeq Stranded Total RNA Kit or NEB Next Ultra™ II Directional RNA Library Prep Kit for Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro transcription was performed with either HiScribe T7 ARCA mRNA kit or the HiScribe In Vitro Transcription Kit (NEB) according to the manufacturer’s recommendation ...
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bioRxiv - Genomics 2023Quote: ... libraries were prepared either with Nugen Ovation Ultralow V2 DNA-Seq library prep kit (NuGen) or NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and areas corresponding to the expected size were excised and purified from the gel using a gel extraction kit (Monarch DNA Gel Extraction Kit, NEB). Following purification ...
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bioRxiv - Microbiology 2024Quote: ... The extracted nucleic acid was then subject to library construction using NEBNext Ultra RNA Library Prep Kit and NEB Next Ultra DNA Library Prep Kit (LTD.NEB, China) for RNA and DNA samples ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted using the QIAzol and the NEB Monarch RNA extraction kit and ribosomal RNA was depleted using the NEBNext rRNA depletion kit (NEB) ...
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bioRxiv - Genetics 2024Quote: ... was extracted via the UHMW DNA extraction protocol of the Nanobind CBB Big DNA kit (Circulomics) or via the Monarch HMW DNA Extraction Kit for Cells & Blood (New England Biolabs) and quantified using Qubit dsDNA Broad Range kit (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was depleted using the Illumina Ribo-Zero Plus rRNA Depletion kit followed by library preparation with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: MNase-seq libraries were prepared from purified nucleosomal DNA using the kit NEBNext Ultra DNA Library Prep Kit for Illumina (cat. # E7370L New England BioLabs) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was isolated using Qiagen RNeasy Plant Mini Kit and cDNA was synthesised using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs). SYBR Green Power Up (Thermofisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Capped RNA was synthesized from the pCS2+hSpCas9 vector using an AmpliCap SP6 High Yield Message Maker Kit (Cell Script) and then purified with a Monarch RNA Cleanup Kit (50 µg) (New England BioLabs).
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bioRxiv - Physiology 2024Quote: ... mRNA sequencing libraries were prepared by OMRF with the xGen RNA Lib Prep Kit (Integrated DNA Technologies) and NEB poly-A selection kit (New England Biolabs). The mRNA libraries were then sequenced as 150 bp paired-end reads on an Illumna NovaSeq 6000 ...
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bioRxiv - Molecular Biology 2024Quote: Libraries were prepared using up to 250 ng of RNA using the NEBNext rRNA Depletion Kit in conjunction with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Deviations from the manufacturer’s protocol include modifications of the recommended 5-fold adaptor dilution to a 40-fold adaptor dilution for RNA inputs between 250ng-10 ng ...
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bioRxiv - Microbiology 2024Quote: ... PCR product and Gel cleanup was performed using Monarch NEB PCR cleanup kit and Monarch NEB Gel extraction kit (New England Biolabs), respectively ...
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bioRxiv - Microbiology 2024Quote: ... the Monarch Gel Extraction Kit and the NEB Monarch PCR Purification Kit (New England Biolabs GmbH, Frankfurt am Main, Germany), according to manufacturer specifications ...
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bioRxiv - Genomics 2024Quote: ... 65℃ for 5 min) using the NEBNext Ultra II FS Library Preparation Kit and primers from the indexed adaptor kit (New England Biolabs). PCR amplified libraries are quantified by spectrophotometer (Lunatic) ...
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bioRxiv - Immunology 2024Quote: Sequencing libraries were prepared using “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” and enriched in mRNA using “NEB Ultra II polyA m RNA magnetic isolation” kit (New England Biolabs). Libraries were pooled and sequenced (single-end ...
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bioRxiv - Neuroscience 2024Quote: ... and RNA was isolated using a commercially available kit (Monarch Total RNA Miniprep Kit; New England Biolabs Inc., cat# T2010S).
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bioRxiv - Molecular Biology 2024Quote: ... Nucleic acid fragments for generation of plasmids were purified either by column purification or by gel extraction (Monarch PCR & DNA Cleanup Kit and Monarch DNA Gel Extraction Kit, New England Biolabs). Fragments were assembled using the NEBuilder HiFi DNA Assembly or applying site-directed mutagenesis (New England Biolabs) ...
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bioRxiv - Genomics 2024Quote: Kits used in the extraction of UHMW DNA were Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB T3050) and Nanobind CBB Big DNA Kit (Circulomics SKU NB-900-001-01 ...
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bioRxiv - Bioengineering 2024Quote: ... or after preparative agarose gel separation by using the Gel Extraction Kit (Peqlab) or the Monarch DNA Gel extraction kit (NEB). The vectors were linearized by restriction digest and dephosphorylated ...
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bioRxiv - Cancer Biology 2021Quote: ... Ligations were performed using Quick Ligation Kit (NEB). Reaction mixes were incubated for 5 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... using Quick Ligation Kit (NEB; Cat. No. M2200L). The ligated DNA was PCR amplified with primers containing Illumina paired-end sequencing adaptors ...
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bioRxiv - Developmental Biology 2021Quote: ... we used the EnGen sgRNA synthesis kit (NEB) following the manufacturer’s instructions with the following targeting sequences tnpo1 sgRNA#1 (5’-GGCATGGGGGCCACCTCTTG-3’) ...
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bioRxiv - Developmental Biology 2021Quote: ... NEBNext Ultra II DNA Library Prep Kit (NEB) with 11 cycles of PCR was used to prepare sequencing libraries ...
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bioRxiv - Microbiology 2022Quote: ... treatment and purification using Monarch Cleanup Kit (NEB). RNA copy number was calculated from its molecular weight and absorbance measured by a NanoDrop 1000 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... PolyA selection/enrichment with Ultra II Kit (NEB) was the method adopted for library preparation.
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bioRxiv - Neuroscience 2021Quote: ... Q5® Site-Directed Mutagenesis Kit (NEB, Inc.) was used to make point mutations to produce five individual EA6-related amino acid substitutions (M128R ...
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bioRxiv - Genomics 2020Quote: ... prepared using the NEBNext kit (New England Biolabs) following the manufacturer’s directions ...
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bioRxiv - Genomics 2020Quote: ... and ribodepleted using the rRNA depletion kit (NEB). The library was then prepared using the NEBNext Ultra II direction Library preparation kit (NEB ...
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bioRxiv - Genomics 2020Quote: ... and the Q5 site-directed mutagenesis kit (NEB) to recreate 4 amino acid changes that were previously found to improve the robustness of tagmentation to lower DNA concentrations (41) ...
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bioRxiv - Molecular Biology 2020Quote: ... blunting with the Quick Blunting™ Kit (NEB), and circularization with T4 Ligase (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... and purified using a PCR purification kit (NEB).
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bioRxiv - Developmental Biology 2020Quote: ... were ligated with Quick Ligation kit (NEB, M2200S). The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... purified using Monarch Gel Extraction Kit (NEB, USA) and ligated with SmaI-digested and dephosphorylated pUC19 vector using Blunt/TA Ligase Mastermix (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... isolated using the Monarch Plasmid preparation kit (NEB) and verified by sequencing (Source Bioscience ...
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bioRxiv - Bioengineering 2021Quote: ... using an NEBuilder HiFi DNA assembly kit (NEB). These manipulations generated px552-pegRNA-CAG-mCherry.
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bioRxiv - Bioengineering 2021Quote: ... and purified using Monarch RNA cleanup kits (NEB).
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bioRxiv - Bioengineering 2020Quote: A Q5 sitedirected mutagenesis kit (New England Biolabs) was used to insert a hexahistidine tag at the N-terminal of the SP141 gene of the P22GFP expression plasmid via standard PCR protocols ...
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bioRxiv - Biochemistry 2021Quote: ... via NEBuilder HiFi DNA Assembly Cloning Kit (NEB). Vector template was amplified using primers oEcoli-C_48 and 49 ...
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bioRxiv - Biochemistry 2020Quote: The Q5 Site directed mutagenesis kit (NEB, E0554S) was used to make the following mutations All mutant constructs were verified by Sanger sequencing.
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bioRxiv - Biochemistry 2020Quote: ... or the Q5 Site-Directed Mutagenesis Kit (NEB). All subcloned PLCε variants contained an N-terminal His-tag and TEV cleavage site ...
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bioRxiv - Synthetic Biology 2021Quote: ... The NEBNext Ultra RNA Library Prep Kit (NEB) was used for RNA sequencing library preparation ...
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bioRxiv - Microbiology 2020Quote: ... Q5 Site-Directed mutagenesis kit (New England BioLabs) was used to generate point mutations in the WT infectious clone ...
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bioRxiv - Microbiology 2020Quote: ... and libraries were constructed using a kit (NEB Ultra DNA library kit for Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... and a reverse transcription kit (NEB, Ipswich, MA) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: ... and Monarch plasmid miniprep kit (New England BioLabs) used for plasmid purification ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 μl of SR RT primer (NEB-kit) and 1.75 μl of nuclease-free water to each sample followed by incubation at 90°C for 30 s ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 μl ProtoScript II RT (NEB-kit) was added to each sample ...