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Citations for New England Biolabs :
1801 - 1850 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... S.aureus Cas9 was amplified with homologous adaptors from pX601 by PCR for ligation into the pMB950 plasmid digested with NheI and XhoI (New England Biolabs).
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bioRxiv - Genetics 2020Quote: ... The ‘2A-Puro-WPRE’ sequence was then removed from the LCv2 plasmid via restriction digestion with PmeI (NEB, Cat# R0560S) and BamHI (NEB ...
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bioRxiv - Genetics 2020Quote: ... the ‘T2A-GFP-WPRE’ sequence was amplified from the hVMD2-hBEST1-T2A-GFP plasmid using LCv2-GFP.Gib.F and .R primers and Q5 2X MM (NEB, Cat# M0492L). The ‘2A-Puro-WPRE’ sequence was then removed from the LCv2 plasmid via restriction digestion with PmeI (NEB ...
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bioRxiv - Genomics 2021Quote: The pCeTyX Ty1hygro-AI carrying plasmid was constructed by assembling four overlapping DNA fragments thanks to the Gibson Assembly Cloning Kit (NEB). The 5,472 bp plasmid backbone was obtained after restriction of p41Neo 1-F GW (https://www.addgene.org/58545/ ...
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bioRxiv - Biophysics 2021Quote: ... The R525E-R527E mutant plasmid was generated using pET-24a-XccBphP as a template for mutagenesis using whole plasmid amplification followed by DpnI template digestion (New England Biolabs). This was achieved using Q5 High-Fidelity DNA Polymerase (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... PCRs purified using the Nucleospin gel and PCR clean-up kit were cloned into plasmid backbones digested with restriction enzymes (NEB) using the In-Fusion HD cloning system (Takara) ...
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bioRxiv - Developmental Biology 2022Quote: ... HDR plasmid with mutant me31B genes was generated by mutating the me31B wild type gene in the wildtype HDR donor plasmid by using the Site-Directed Mutagenesis kit (New England Biolabs) according to the manufacturer’s recommended protocols ...
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bioRxiv - Cell Biology 2022Quote: MKLP1(1-711)-3xFLAG-Avi was cloned by stitching four oligonucleotide primer sequences together into a digested MKLP1(1-711)-Avitag plasmid using a HiFi DNA assembly reaction kit (M5520A, New England Biolabs). Ten 10cm plates were seeded with COS-7 cells and each plate was co-transfected 24 hours later with 4.08 μg MKLP1(1-711)-3xFLAG-Avi and 4.08 μg HA-BirA plasmids using TransIT-LT1 transfection reagent (Mirus) ...
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bioRxiv - Molecular Biology 2022Quote: ... RD fragments amplified from Drosophila embryonic cDNA were integrated between SgrDI (Thermo) and BsHTI (Thermo) restriction sites in the respective backbone plasmid via Gibson assembly (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The new construct was created from a PCR reaction that amplified the entire plasmid harbouring the substitution using the high-fidelity polymerase Q5 according to the manufacturer’s instructions (New England Biolabs; NEB). The unpurified PCR product was then treated with DpnI to digest the methylated template DNA which was subsequently transformed into DH5α ...
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bioRxiv - Biophysics 2022Quote: ... the assembled sequences were transferred to the target plasmids (WT, MUT) by using a different set of type IIS enzymes (BbvI, New England Biolabs #R0173 ...
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bioRxiv - Bioengineering 2022Quote: ... A 4699 bp HDR template dsDNA (HDRT) was generated by amplification from a plasmid template using the Q5 High-Fidelity 2X Master Mix (New England BioLabs) with primers containing truncated Cas9 target sequences (IDT ...
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bioRxiv - Bioengineering 2022Quote: All rAAV6 vector plasmids were cloned using NEBuilder® HiFi DNA Assembly Master Mix (cat # E2621L, New England Biolabs (NEB) Inc. ...
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bioRxiv - Bioengineering 2022Quote: All rAAV6 vector plasmids were cloned using NEBuilder® HiFi DNA Assembly Master Mix (cat # E2621L, New England Biolabs (NEB) Inc. ...
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bioRxiv - Biochemistry 2022Quote: Elp constructs were PCR amplified with encoded 5’ BamHI and 3’ NotI restriction sites from the previously generated full-length ElpQ19-343 and ElpB19-378 pT7HMT plasmids using Q5 Master Mix (NEB) as previously described (31) ...
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bioRxiv - Cell Biology 2022Quote: ... To generate pcDNA5/FRT-Tg-NLuc plasmids the Tg gene was amplified from the pcDNA5/FRT-Tg-FLAG plasmid and assembled with the NLuc fragment using a HiFi DNA assembly kit (New England BioLabs). To generate the respective mutant construct plasmids for A2234D and C1264R Tg ...
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bioRxiv - Immunology 2022Quote: Protein expression plasmids were constructed using dsDNA gBlocks from IDT and NEBuilder® HiFi DNA Assembly Cloning Kit (NEB, E5520S) and then transformed in NEB 5-alpha competent cells (NEB ...
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bioRxiv - Genetics 2022Quote: ... I23A was introduced at the same time with GFP knock-in by incorporating the corresponding mutation in the 3’ homology arm on the repair template plasmid using the Q5 site-directed mutagenesis kit (New England Biolabs). GermLine Optimized mScarlet-i sequence (Fielmich et al ...
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bioRxiv - Genomics 2022Quote: ... Ligand-encoding sequences were subsequently cloned by restriction digest removal of CD19-encoding sequence from the EF1ɑ-CD19 IRES-EGFP lentiviral plasmid using XbaI & MluI (New England Biolabs) and inserted with InFusion cloning (Takara Bio) ...
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bioRxiv - Genomics 2022Quote: A second-generation lentiviral transfer plasmid encoding expression of EF1ɑ promoter - CAR-P2A-mCherry (21) was digested with XbaI & MluI (New England Biolabs) to drop out the CAR-P2A-mCherry transgene ...
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bioRxiv - Immunology 2022Quote: Fab fragments were generated by inserting a stop codon six amino acids upstream of the hinge region (CPPCP) of the heavy chain expression plasmid using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). This mutagenized plasmid was co-transfected with the respective light chain plasmid ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 40bp downstream) were PCR-amplified from the PN16 (IncI2) plasmid using Q5® High-Fidelity DNA Polymerase (New England BioLabs). The amplified fragment was cloned into pSEVA121 using the NEBuilder® HiFi DNA Assembly kit (New England BioLabs ...
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bioRxiv - Genomics 2022Quote: PCR templates for in vitro transcription (IVT) were amplified from S2 cell cDNA or pGF-P5(S65T) plasmid using Phusion Hot Start Flex DNA Polymerase (NEB) and primers listed in Supplementary Table 5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Other G2L4 and GII RT mutant plasmids were derived from pMal-G2L4 RT or pMal-GII RT by using a Q5 mutagenesis kit (New England Biolabs).
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bioRxiv - Molecular Biology 2022Quote: ... The PCR was done with 1-2 ng of plasmid and 200 nM of each primer in Phusion High-Fidelity PCR Master Mix (New England Biolabs) with pre-denaturation at 98°C for 5 sec followed by 12 cycles of 98°C for 5 sec ...
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bioRxiv - Microbiology 2022Quote: ... These vectors were generated by mutating the sgRNA site in the pSag1-Cas9-U6-sgUPRT-HXG plasmid [14] using the Q5 Site-Directed Mutagenesis Kit (NEB). The two guide RNAs were designed to target TgEFP1 at either of the predicted EF-hand domains using the online E-CRISP tool (http://www.e-crisp.org/E-CRISP/) ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid backbones and inserts for cloning were amplified from the purified chromosomal DNA using Q5 High-Fidelity DNA Polymerase (NEB). PCR products and plasmids were purified with Monarch DNA kits (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... The other molecular cloning trials and plasmid gene replacements were performed by Gibson’s assembly with Gibson Assembly® Cloning Kit (NEB). The procedure used to generate each plasmid construct is described in the corresponding subsection of Material and Methods.
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bioRxiv - Microbiology 2022Quote: ... followed by gel-purification of the plasmid backbone and Gibson assembly with re-targeted intron fragments using the Gibson Assembly Master Mix (NEB). Gibson assemblies were transformed into E ...
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bioRxiv - Synthetic Biology 2022Quote: ... Multilayer genetic circuit plasmids were constructed using PCR and Golden Gate assembly (GAA) using BsmBI Type IIS Enzyme (New England Biolabs), and sRNA plasmids using BsaI Type IIS Enzyme (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... The fragments were purified and inserted into pCAMBIA3300U (pCAMBIA3300 with a double PacI USER cassette inserted between the PstI-XmaI sites at the multiple cloning site) (Nour-Eldin et al, 2006) after which the plasmid was transformed into Escherichia coli DH5α (NEB). Kanamycin resistant colonies were selected and their plasmids purified followed by restriction digestion and sequencing prior to introduction into Agrobacterium tumefaciens GV3101 for plant transformation ...
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bioRxiv - Neuroscience 2022Quote: ... The digested plasmid and the PCR product were then assembled with NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs). Chemically competent Stbl3 Escherichia coli cells (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: 5’ biotinylated DNA that was used for transcription antitermination assays and as a PCR template when preparing modified DNA templates below was PCR amplified from plasmid DNA using Q5 High-Fidelity DNA Polymerase (New England Biolabs) and primers HP4_5bio.R and PRA1_NoMod.F (for ZTP and fluoride templates ...
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bioRxiv - Plant Biology 2022Quote: ... The sgRNA expression cassettes of OsU6c–sgRNA–T1 and OsU3–sgRNA–T2 were amplified from pYLsgRNA–OsU6c and pYLsgRNA–OsU3 plasmids using Phusion High-Fidelity DNA Polymerase (New England BioLabs) and cloned into a binary vector ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplified yciF (with Strep II tag on C-terminal) and pQE60 plasmid (containing an IPTG inducible T5 promoter) were digested with BamHI and EcoRI (New England Biolabs) and purified by gel extraction (MinElute gel extraction kit ...
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bioRxiv - Genomics 2022Quote: All plasmids were constructed using standard cloning methods with New England Biolabs restriction enzymes and T4 DNA ligase (New England Biolabs) or with the NEBuilder HiFi DNA Assembly kit (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... melanogaster strain to be injected and inserted into plasmids containing fluorescent eye markers using NEBuilder Hi-Fi DNA assembly (NEB). See key resources table for primers sequences and donor plasmids.
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bioRxiv - Microbiology 2022Quote: ... FastDigest restriction enzymes (Thermo-Fisher) were used for plasmid digestion and Gibson assembly was performed using the NEBuilder HiFi DNA assembly kit (NEB). All constructs were verified by DNA sequencing.
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bioRxiv - Synthetic Biology 2022Quote: A double-stranded DNA scaffold template was obtained from pUCIDT-AMP-T7p_DB982 plasmid (10 ng in 50 μL reaction mixture) by PCR amplification using Phusion® DNA polymerase (NEB), T7-DB982 forward and T7-DB982 reverse primers (0.5 μM each) ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled between the FRT sites of plasmid pKD4 by gibson assembly using NEBuilder HiFi DNA Assembly master mix (NEB). 2x tRNA cassettes integration plasmids were constructed from a 2x-tRNA construct under the control of a proK tRNA promoter and terminator and separated by a valU tRNA linker that was ordered as a DNA fragment from Genewiz ...
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bioRxiv - Molecular Biology 2022Quote: ... and subsequently cloned via Gibson Assembly in the pRRL-EF1a-XhoI-IRES-BlastR plasmid (gift from G. Superti-Furga) using the NEBuilder HiFi DNA Assembly Mix (New England Biolabs). The CRBN/VHL WT and point mutant plasmids were used for lentivirus production and subsequent transduction in RKO CRBN-/- and VHL-/- clones respectively.
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bioRxiv - Molecular Biology 2022Quote: ... IVT RNA products (2×10-3 dilution) were tested for absence of carry-over plasmid template by PCR using Taq 2X master mix (NEB). A negative PCR result would confirm the absence of carryover plasmid in the IVT product.
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bioRxiv - Molecular Biology 2022Quote: ... were generated by deletion of the unwanted DNA from the parental plasmid using the Q5 Site-Directed Mutagenesis Kit (NEB BioLabs). The R763G point mutation was introduced into D1-A1 by PCR mutagenesis ...
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bioRxiv - Plant Biology 2022Quote: ... DNA fragments into plasmid pODC53 (Caspari, 2020) upstream of Venus using Gibson assembly (NEBuilder HiFi DNA assembly, #E5520S, New England Biolabs). Chlamydomonas TP sequences were amplified from genomic DNA extracted from strain T222+ (CC-5101) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A donor DNA containing two homology arms flanking the DsRed sequence for homology repair were cloned into the pHD-ScarlessDsRed plasmid (https://flycrispr.org/scarless-gene-editing/) using the Q5® High-Fidelity DNA Polymerase kit (New England Biolabs). Left homology arm contained the sequence in 2R:9870299-9871095 (FlyBase Release 6 ...
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bioRxiv - Plant Biology 2022Quote: ... gRNA modules were combined with pGG-C-linker-G plasmid and cloned into pEN-R2-A-G-L3 by restriction-ligation using BsaI enzyme (New England Biolabs) to obtain pEN-R2-gRNA_BRI1-3-gRNA_BRI1-2-L3 ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... 5 µl of the plasmid DNA served as template for a 50 µl PCR (Q5 High-Fidelity 2X Master Mix, NEB) with primers PK412+PK421 (input library ...
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bioRxiv - Molecular Biology 2023Quote: ... these antibiotic resistant cassettes were excised by transforming the strains with a plasmid expressing the Cre recombinase (pDR244, BGSCID: ECE274) purified from RecA+ Escherichia coli (NEB) cells with the GeneJET Plasmid Miniprep Kit (Thermo) ...
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bioRxiv - Molecular Biology 2022Quote: ... following the manufacture’s protocol. Plasmids were transformed in DH5-alpha or DH10-beta chemo-competent Escherichia coli (E. coli) cells (New England Biolabs). Transformed bacteria were grown in LB medium supplemented with 50 μg/mL kanamycin or 100 μg/mL carbenicillin ...
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bioRxiv - Biophysics 2022Quote: ... The PCR product was digested by HindIII and NheI then was cloned into the digested pGJJ162 plasmid using T4 Ligase (NEB). To construct 6 KRAS BindingPCA plasmids ...