Labshake search
Citations for New England Biolabs :
1901 - 1950 of 2293 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... The PCR products were gel-purified and then assembled into a plasmid backbone digested with MluI (New England BioLabs, R3198S) and SpeI (New England BioLabs ...
-
bioRxiv - Developmental Biology 2022Quote: ... PCR reaction and digested plasmid were gel purified and used to perform a Gibson Assembly reaction (Cat. #E2611S, New England Biolabs) followed by bacterial transformation according to the manufacturer instructions ...
-
bioRxiv - Immunology 2023Quote: ... The CAR backbone constructs were ordered as gBlock (IDT) and cloned into the LZRS-IRES-eGFP retroviral plasmid by Gibson Assembly (NEBuilder HiFi DNA Assembly Master Mix) (NEB). The VHH specific sequence was amplified using PCR (Phusion High Fidelity PCR kit ...
-
bioRxiv - Developmental Biology 2023Quote: ... The loxP-dsRed-loxP fragment and the above modified pJet1.2 plasmid were digested with the restriction enzymes AgeI (NEB, R0552S) and PacI (NEB ...
-
bioRxiv - Molecular Biology 2023Quote: ... have been obtained by gBlock gene fragments synthesis (IDT) and subsequently cloned into a plasmid backbone via Gibson assembly protocol (New England Biolabs) following manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: Control and DRP1 gRNA were cloned into a plasmid containing a U6 promoter using BstXI and BlpI restriction enzymes (NEB) (gifted by Dr ...
-
bioRxiv - Biochemistry 2023Quote: ... Hrd1 was mutagenized across the entire coding sequence and cloned into a centromeric plasmid using HiFi Assembly (New England BioLabs). The centromeric Hrd1 library was transformed into hrd1Δ expressing substrates from centromeric plasmids and plated ...
-
bioRxiv - Biochemistry 2023Quote: The generation of all plasmids was performed using NEBuilder HiFi DNA assembly Master Mix (New England Biolabs GmbH, Frankfurt, Germany). Protein coding sequences (CDS ...
-
bioRxiv - Biochemistry 2023Quote: ... then ligated into linearized plasmid backbones using an NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs Cat. No. E5520S). Recombinant plasmids were transformed into E ...
-
bioRxiv - Microbiology 2023Quote: ... Secreted cypridina Luciferase (cLuc) activity from the internal control plasmid was similarly measured using the cypridina Luciferase kit (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... the synthesized S100A11 was removed from the pEX-A128 plasmid by restriction digestion with XhoI and SacII (New England Biolabs), then cloned into a pTK14-GFP plasmid9 ...
-
bioRxiv - Molecular Biology 2023Quote: ... under its native promoter was cloned into the plasmid pACYC184 (p15A origin of replication) using Gibson assembly (New England Biolabs) generating plasmid JW371 ...
-
bioRxiv - Cell Biology 2023Quote: ... flag tag and SunTag reporter were cloned from the SINAPs construct to replace the CMV promoter and tdTomato reporter of the tdTomato-FL2 plasmid using NEBuilder HiFi DNA Assembly technology (NEB).
-
bioRxiv - Developmental Biology 2023Quote: ... we cloned the Grx1-roGFP2 sequence from the “pLPCX mito Grx1-roGFP2” plasmid into a lentiviral vector under the EF1 promoter “pCDH-EF1” using Gibson Assembly (NEB) (primers described in Table 1) ...
-
bioRxiv - Developmental Biology 2023Quote: ... The resulting PGEMT-BFP-Lamp1-P2A-MLS-mKate2-T2A-EGFP-Tubulin plasmid was sequence verified and digested with MluI (R0198, New England Biolabs) to replace the tdTomato sequence in Ai9 ...
-
bioRxiv - Developmental Biology 2023Quote: One cell stage AB/TU embryos were injected with 75 pg of smyhc1:gfp plasmid (gift from Stone Elworthy, University of Sheffield, Sheffield, United Kingdom) previously digested with meganuclease ISceI (Biolabs) in order to label slow muscle cells ...
-
bioRxiv - Synthetic Biology 2023Quote: ... A plasmid harboring Cas9 and an empty guide RNA transcription cassette was linearized by NotI/BsaI double digestion (New England Biolabs) and transformed into yeast together with a linear piece of DNA containing the guide RNA sequence flanked on either side by homology to the plasmid ...
-
bioRxiv - Plant Biology 2023Quote: ... using sequence-specific primers and introduced upstream of the firefly luciferase gene (LUC) in the pGreenII 0800-LUC plasmid (55) by a NEBuilder HiFi DNA Assembly reaction (New England Biolabs) to create the proSyn35S5xUASGAL:LUC plasmid also containing a pro35S:REN cassette ...
-
bioRxiv - Systems Biology 2024Quote: ... first a highly active HIS4-targeting sgRNA was cloned into D-227 vector by digesting the plasmid with BbVCI enzyme (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... Barcodes were amplified from cDNA or input plasmid DNA using 24 cycles of PCR with Phusion High Fidelity Master Mix (NEB). Amplicons were purified with the Monarch PCR kit (NEB) ...
-
bioRxiv - Synthetic Biology 2024Quote: All replicative plasmid backbones were constructed using the Golden Gate Assembly strategy with the type II restriction enzyme Esp3I (NEB) according to the manufacturer’s protocol (Table 1) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The DNA template used for in vitro transcription was generated by PCR amplification from the corresponding reporter plasmid using the Q5 High-Fidelity DNA Polymerase (NEB) with a reaction including a forward primer containing the T7 promoter sequence and a 60T reverse primer for polyadenylation ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: The construct pcDNA4TO-TwinStrep (TS)-SNAP-β2AR was generated by amplification of the SNAP and β2AR sequences of the pSNAPf-ADRB2 plasmid (NEB) and inserted into pcDNA4TO-TS using Gibson assembly (Heydenreich et al. ...
-
bioRxiv - Microbiology 2023Quote: ... RHCas9 Δgra72 parasites were co-transfected with plasmids containing sgRNAs specifically targeting the UPRT locus and SacI (New England Biolabs)-linearized pTwist-CMV: ...
-
bioRxiv - Microbiology 2023Quote: ... RH-Luc+ Δgra47 or ME49-cLuc+ Δgra47 were co-transfected with plasmids containing sgRNAs specifically targeting the UPRT locus (Table S1) and EcoRV (New England Biolabs)-linearized pUPRT::GRA47HA plasmid at a ratio 1:5 of sgRNAs to linearized plasmid ...
-
bioRxiv - Microbiology 2023Quote: ... we co-transfected the pU6-Universal plasmid carrying a sgRNA targeting the gene of interest (Table S1) and the BamHI-HF (New England Biolabs) linearized pTKOatt plasmid ...
-
bioRxiv - Microbiology 2023Quote: Genes of interest were PCR-amplified with 25 bp homology arms from high-titer phage lysates and ligated into respective plasmid backbones using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). Recombinant plasmids were transformed into E ...
-
bioRxiv - Molecular Biology 2023Quote: ... and inserted into EasyClone plasmid pCfB2226 by USER cloning upstream of the ADH1 terminator with USER Enzyme Mix (NEB, M5508) according to (Stovicek et al ...
-
bioRxiv - Microbiology 2023Quote: ... HMBS_232 was cloned into pCMV-puroR lentiviral plasmid using the primers listed in Table S6 and NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs). Lentiviruses were produced as already mentioned ...
-
bioRxiv - Biophysics 2023Quote: ... by site-directed mutagenesis of the HT-rad21 and HT-CTCF plasmids using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) according to the manufacturer’s protocol with primers given in Supplementary Table 18 ...
-
bioRxiv - Biochemistry 2023Quote: ... The central part of the DNA construct was obtained by digestion of a large homemade plasmid with BssHII (New England Biolabs) produced following published protocols (54) ...
-
bioRxiv - Biochemistry 2023Quote: ... were codon-optimized for bacterial expression and cloned into the pET15b plasmid using the Gibson assembly method (New England Biolabs).
-
bioRxiv - Bioengineering 2023Quote: The PCR reaction was performed and the resulting PCR product as well as PCDH-mNeonGreen-MCS-T2A-puromycin plasmid were cleaved with BamH1(NEB) and Not1(NEB ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 μg of the library plasmid was digested at 37 °C for 5 hours with NdeI-HF and SacI-HF (New England Biolabs). Then ...
-
bioRxiv - Molecular Biology 2023Quote: ... two independent 50 μL reactions with 1 μg of backbone plasmid were digested at 37 °C for 1 hour with MluI-HF and EcoRI-HF (New England Biolabs). After heat-inactivation (65 °C ...
-
bioRxiv - Biophysics 2023Quote: ... A vector backbone was generated using pHR-dSV lentiviral plasmid digested with Mlu1 and Not1 restriction enzymes (New England Biolabs). Construction of the pHR-dSV-TMEM-PhoCl-IRAK4-mScarlet fusion was achieved by Gibson Assembly of the three components.
-
bioRxiv - Bioengineering 2024Quote: ... the coding sequence of PE2 and PEmax were cloned into the mRNA production plasmid using HiFi DNA Assembly Master Mix (NEB). mRNAs were transcribed to contain 101 nucleotide-long poly(A ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 10 ng of the intermediate plasmid products were incubated with 10 uM random hexamer primers and 1X phi29 DNA polymerase buffer (NEB) at 95°C for 3 minutes and then cooled to room temperature ...
-
Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... 2018).Plasmid and inserts were digested with KpnI and BglII or SacII restriction enzymes (New England Biolabs, Ipswich, MA, USA) following manufacturer instructions ...
-
bioRxiv - Genomics 2024Quote: ... 1 μg of each sublibrary plasmid was digested at 37°C for 5 hours with NheI-HF and SacI-HF (New England Biolabs), incubated with rSAP for 30 minutes at 37°C ...
-
bioRxiv - Microbiology 2024Quote: ... 25 ng of linearised plasmids were ligated with 75 ng of purified VH and VL by Gibson Assembly (New England BioLabs). The reaction was performed in a final volume of 5 μL ...
-
bioRxiv - Systems Biology 2024Quote: Approximately 6ng of each loxcode cassette was combined with approximately 75ng of the linear digested plasmid pool in a final 10uL of 1x HiFi assembly reaction (NEB). This was incubated on a thermocycler at 50C for 15min ...
-
bioRxiv - Bioengineering 2023Quote: ... The PCR fragment was gel purified and inserted into plasmids pTRC99a and pKI_IS10 (gift of S. Wenk) using HiFi DNA Assembly Cloning Kit (NEB. pZE21) for Gibson cloning ...
-
bioRxiv - Biophysics 2023Quote: ... with the restriction enzymes AvrII and HindIII and cloned into the digested plasmid pGJJ191 (described in 24) using T4 ligase (New England Biolabs). AbundancePCA (aPCA ...
-
bioRxiv - Genetics 2023Quote: ... Homology arms were obtained by PCR from HEK-293T genomic DNA and a plasmid template was generated per candidate GSH site by Gibson assembly (New England Biolabs) using fragments synthesized by Q5 High Fidelity Taq polymerase (New England Biolabs) ...
-
bioRxiv - Developmental Biology 2023Quote: ... The fragments were cloned into a pCS2+ plasmid linearized with Xho1 and Xba1 restriction enzymes using a Quick Ligation Kit (NEB). Each plasmid was then linearized with NotI restriction enzyme and mRNAs were synthesized by in vitro transcription (using the mMESSAGE mMACHINE sp6 Ultra kit AM1340 ...
-
bioRxiv - Genomics 2023Quote: ... The backbones for the Gibson reaction for GRB2-SH3 and PSD95-PDZ3 library assembly (aPCA plasmids) were first linearized using primers listed in Extended Data Table 3 and next treated with Dpn1 (NEB) restriction enzyme to remove the circular plasmid template ...
-
bioRxiv - Developmental Biology 2023Quote: ... The Sox2(600bp)::GFP plasmid was generated by digesting the primer overhangs of the PCR product with XhoI and EcoRV (NEB) and its consequent ligation into linearised plasmid ISceI/MCS-d1GFP-SV40/ISceI ...
-
bioRxiv - Systems Biology 2024Quote: ... We digested ∼2ug of each of 24 barcoded ORF plasmid pools (2 replicate pools of each of 9 hORFs and 3 vORFs) overnight with I-SceI (NEB) according to manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2024Quote: ... were generated from a pENTR cyfip2-EGFP plasmid [32] using custom primers and the Q5 Site Directed Mutagenesis Kit (NEB) to induce the desired C179R (ΔRac1 ...