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Citations for New England Biolabs :
1301 - 1350 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... PCR was performed with 2x NEBNext High-Fidelity PCR Master Mix (New England Biolabs, M0541S) and 10 μM primers (Table S2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were generated by PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB). The resulting libraries were multiplexed and sequenced in a HiSeq 4000 paired-end lane ...
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bioRxiv - Genomics 2023Quote: ... The outward PCR experiments were performed using NEBNext High-Fidelity 2X PCR Master Mix (NEB). For a 50 µl reaction ...
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bioRxiv - Genomics 2023Quote: ... pooled oligonucleotides were amplified via PCR using NEBNext 2X Hi-Fi PCR Master Mix (NEB) and primers:
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were generated by PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB). The resulting libraries were purified using MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: PCR and plasmid generation – All PCR for plasmid generation was conducted using Phusion polymerase (NEB) using primers listed in Supplemental Table 1 ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA was then PCR-amplified using barcoded PCR primers (New England Biolabs #E7335L, #E7500L). After AMPure XP bead purification (Beckman Coulter #A63881 ...
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bioRxiv - Systems Biology 2023Quote: ... PCR amplification was carried out in a reaction mixture containing PCR Master Mix (NEB, M0543S), 0.5 μM of each primer ...
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bioRxiv - Neuroscience 2023Quote: ... PCR amplified Domain 1 and PCR Domain 2 and HiFi DNA assmbley was performed (NEB). Transformation was performed in DH5α cells (REF) ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was then PCR-amplified using barcoded PCR primers (New England Biolabs #E7335L, #E7500L). After AMPure XP bead purification (Beckman Coulter #A63881 ...
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bioRxiv - Developmental Biology 2023Quote: ... After samples were PCR-amplified using 1×NEBNext High-Fidelity PCR Master Mix (NEB, M0541S). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Physiology 2024Quote: ... After the PCR 20ul of the PCR product were restriction digested with BamH1 (NEB #xxx) for 1 hr at 37C ...
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bioRxiv - Genomics 2020Quote: ... ‘GunIt’ refers to the release (dx.doi.org/10.17504/protocols.io.bdp7i5rn) and ‘LoCost’ includes LunaScript RT SuperMix (NEB) instead of SuperScript IV (Thermo ...
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bioRxiv - Molecular Biology 2021Quote: 5’ RACE was performed using the Template Switching RT Enzyme Mix (New England Biolabs M0466) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2020Quote: The following components comprised the RT-LAMP assay: 4 mM of MgSO4 (New England Biolabs), 1× final concentration of the isothermal amplification buffer (New England Biolabs) ...
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bioRxiv - Genetics 2021Quote: ... RT-qPCR analysis was performed in Luna Universal qPCR Master Mix (New England Biolabs, #M3003) using a Roche LightCycler480 (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... we treated the purified RT reaction with 1 μl of Exonuclease I (NEB, Catalog# M0293L) at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μl RT mastermix (2x reaction buffer, 20 mM DTT, 4U murine RNase inhibitor [NEB] ...
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bioRxiv - Immunology 2023Quote: ... total RNA was reverse transcribed into cDNA with LunaScript RT Supermix (New England Biolabs, E3010) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... For AsiSI digestion, samples were equilibrated (2 min, RT) in 150 µL CutSmart buffer (NEB). 3µl recombinant AsiSI endonuclease (10U/µL ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed on 0.5 µg DNase-treated total RNA using Lunascript RT (NEB) in 20µl reactions ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 1 µg RNA was reverse transcribed to cDNA (LunaScript RT SuperMix, New England BioLabs, USA).
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bioRxiv - Developmental Biology 2023Quote: ... and RT-qPCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs). The comparative 2-ΔΔCT method was used to determine relative expression of the G9a cKO samples to compared the expression level of controls ...
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bioRxiv - Cell Biology 2023Quote: ... Primer extension was catalyzed by 50 units of Protoscript II RT (New England Biolabs, M0368) in a 5 µL reaction containing 0.5 mM dNTP ...
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bioRxiv - Microbiology 2020Quote: ... The T7-3M-eGFP-5M minigenome was made by replacing the Renilla luciferase gene in the T7-3M-Luc-5M minigenome with an eGFP gene through assembly of PCR fragments using the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs, MA, USA). A modified version of this minigenome ...
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bioRxiv - Microbiology 2019Quote: ... and 7 cycles of PCR to enrich for ligated product was done with NEBNext® Ultra™ DNA Library Prep Kit for Illumina (New England Biolabs). Libraries were quantitated with KAPA Library Quantification Kits for Next-Generation Sequencing (Roche Sequencing Solutions ...
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bioRxiv - Microbiology 2019Quote: ... 10 (GSF1697) or 12 (GSF2248) PCR cycles were run using 8-nt barcoded oligos from NEBNext Multiplex Oligos barcode kit (NEB cat# 6609S). The libraries were purified with Agencourt AMPure XP beads ...
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bioRxiv - Microbiology 2020Quote: ... we separated the PCR products from the parental genomic DNA by gel electrophoresis and purified the PCR fragments using the Monarch DNA Gel Extraction Kit (New England Biolabs, Evry, Fr). The purified fragments were then mixed and fused together using assembly PCR carried out with the Q5 high-fidelity DNA polymerase ...
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bioRxiv - Neuroscience 2020Quote: ... Fragmentation and library construction of the resulting PCR products were performed using an NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the purified cDNA was PCR amplified and barcoded using ONT’s PCR Barcoding Expansion 1-12 (EXP-PBC001) or PCR Barcoding Expansion 1-96 (EXP-PBC096) kits with LongAmp Taq 2x Mastermix (NEB, Ipswich, MA).
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bioRxiv - Genomics 2022Quote: ... and a PCR-free library was obtained with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs). Approximately 200 million of 150 bases pair-end reads were generated using the HiSeq X System (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... and one fragment of about 320 bp of the 3’-terminal region by 3’ RACE were amplified using Phusion High-Fidelity PCR Kit (New England Biolabs, MA, USA) under the following conditions [98°C ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed in a two-step manner using a ProtoScript First Strand cDNA synthesis kit (New England Biolabs, Cat#E6300S) and iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... mutated FN constructs were PCR amplified and ligated into BamH1+Xho1 cut NSPI vector using NEBuilder® HiFi DNA Assembly kit (BioLabs, #E5520).
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bioRxiv - Microbiology 2019Quote: ... each of the amplicons were diluted to 0.5 nM and amplified using barcoding primers (1D PCR Barcoding Amplicon Kit, Oxford Nanopore Technologies, [ONT], UK) and LongAmp Taq 2X Master Mix (New England Biolabs, MA, USA) with the following conditions ...
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bioRxiv - Synthetic Biology 2020Quote: ... were PCR amplified from genomic DNA of yeast strain BY474129 and cloned together with the bidirectional inducible Gal1-10 promoter into the PCR amplified pRSII42B backbone with OZL14 and OZL15 via Gibson assembly30 using NEBuilder Kit (NEB, catalog E2621). MMR-DN mutant genes were subsequently generated via site-directed mutagenesis using either Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... IVT reactions were performed using the HiScribe T7 High Yield RNA Synthesis Kit using 300 ng of PCR product as template (NEB Cat. #E2040S). After an incubation for 16 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... plus 280 nM for each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs, Ipswich, MA). Samples were then cycled under the following qPCR conditions ...
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bioRxiv - Immunology 2021Quote: ... The concentration of libraries was determined by quantitative PCR using a NEBNext library Quant kit for Illumina (New England BioLabs, Ipswich MA). The size of libraries and amplicon profiles were determined by the Fragment Analyzer System (Agilent Technologies ...
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bioRxiv - Genomics 2021Quote: ... DNA was diluted ten-fold and used as template for the second round of PCR where Illumina adaptors for sequencing were added using the NEBNext® Multiplex Oligos Illumina dual index kit (NEB, UK) with 10 cycles of 10 seconds at 98°C ...
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bioRxiv - Immunology 2022Quote: ... and unc-54 3’utr pieces were Gibson assembled using the NEBuilder assembly kit and the assembled product was then PCR-amplified with Phusion DNA polymerase (New England Biolabs, Ipswich, MA). The PCR-amplified assembly was A-tailed for 30 min at 70 °C ...
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bioRxiv - Neuroscience 2022Quote: ... the 181-bp and 376-bp amplicon products (Figure 1-figure supplement 1A) were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) and sent for Sanger DNA sequencing at the OHSU Vollum Sequence Core (Portland ...
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bioRxiv - Molecular Biology 2023Quote: ... gDNA from cell pellets was isolated using the Quick-DNA Midiprep Plus Kit (ZymoResearch, D4075) and genome-integrated sgRNA sequences were amplified by PCR using the Q5 Mastermix (New England Biolabs Next UltraII). i5 and i7 multiplexing barcodes were added in a second round of PCR ...
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bioRxiv - Genomics 2023Quote: ... The PCR2 product pools were subjected to three steps of purification with the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L), Gel DNA Recover Kit (Zymoclean ...
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bioRxiv - Plant Biology 2023Quote: ... pCAMBIA0380 was digested by EcoRI and XhoI and used for the cloning of three PCR fragments with Gibson Assembly cloning kit (NEB, Evry, France) according to the supplier’s indications ...
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bioRxiv - Bioengineering 2023Quote: ... was amplified by PCR from the same vector and its DNA was extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs, Ipswich, MA). GLuc was inserted into the digested backbone through Gibson Assembly Hi-Fi kit (New England Biolabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... DpnI treated PCR products were purified with silica column (FastGene Gel/PCR Extraction Kit, Nippon Genetics Co., Ltd., Tokyo, Japan) and then HiFi assembly reaction (New England Biolabs, Ipswich, MA) was carried out to connect each end of linear DNA products ...
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bioRxiv - Molecular Biology 2021Quote: ... Desired PCR cycle numbers were determined in test endpoint PCRs using Q5 DNA polymerase (NEB M0491L). Final HiC libraries were generated from 4-6 individual PCR reactions ...
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bioRxiv - Genetics 2021Quote: ... and libraries were generated by PCR with NEBNext High Fidelity 2X PCR Master Mix (NEB M0541). Library size was confirmed on a BioAnalyzer prior to sequencing on the NextSeq 550 (40bp read length ...
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bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR MasterMix (New England Biolabs). The PCR products examined with 400-450bp were chosen and mixed in equal density ratios ...