Labshake search
Citations for New England Biolabs :
1451 - 1500 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... Each reverse transcription reaction contained 8 μL template RNA and 2 μL LunaScript RT SuperMix (NEB #E3010). The reaction condition for reverse transcription was ...
-
bioRxiv - Cancer Biology 2021Quote: ... The regular PCR was performed using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs). The PCR products that targeted exon18-10 and exon9-10 were then combined for each sample and run on 2% agarose gel ...
-
bioRxiv - Developmental Biology 2021Quote: ... The PCR reactions (total 30 μL) included 15 μL PhusionR High-Fidelity PCR Master Mix (New England Biolabs), 0.2 mM primers ...
-
bioRxiv - Genomics 2021Quote: ... The PCR reaction consisted of 25 µL NEBNext Hi-Fidelity 2x PCR Master Mix (New England Biolabs NEB.M0541S), 7.5 µL H2O ...
-
bioRxiv - Genomics 2021Quote: ... The PCR reaction consisted of 25 µL NEBNext Hi-Fidelity 2x PCR Master Mix (New England Biolabs NEB.M0541S), 7.5 µL H2O ...
-
bioRxiv - Molecular Biology 2021Quote: ... PCR amplification was performed in 1X Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB M0531) and PCR cycling at 98°C for 30s ...
-
bioRxiv - Plant Biology 2021Quote: ... transposed DNA was PCR amplified with 12 cycles using Next High-Fidelity 2×PCR Master Mix (NEB, M0541) with Nextera DNA CD Index primers ...
-
bioRxiv - Immunology 2022Quote: ... Final amplification PCR was performed as described before by adding 8 µl of 2 x PCR MM (NEB) with number of cycles according to qPCR (usually Cq values were around 20) ...
-
bioRxiv - Neuroscience 2020Quote: ... PCR amplification was performed for 15 cycli using the NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541), followed by a 0.8x beads clean-up ...
-
bioRxiv - Microbiology 2019Quote: ... All the PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs). The libraries generated with TruSeq DNA PCR-Free Sample Preparation Kit were sequenced using paired-end Illumina sequencing (2 × 250 bp ...
-
bioRxiv - Synthetic Biology 2019Quote: ... PCR reactions were performed with the Phusion High-Fidelity PCR Master Mix with HF buffer (New England Biolabs). All inserts were verified by sequencing (GENEWIZ) ...
-
bioRxiv - Genomics 2020Quote: ... The final PCR amplification was performed using 2X Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) and final 0.1 μM of PE1.0 and corresponding multiplex PE2.0_MTX (Table S3) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Tandem PCR was performed by Q5 PCR (0.5 μl Q5® HiFi DNA Polymerase (New England Biolabs, M0491), 1x Q5 reaction buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... 11) with 15 cycles of PCR using NEBNext® Q5® Hot Start HiFi PCR Master Mix (NEB), which allowed to add Gap Repair recombination sequences for the cloning in Gal4-AD prey plasmid pP7 ...
-
bioRxiv - Immunology 2019Quote: ... the PCR product was subjected PCR using Q5® Hot Start High-Fidelity 2X Master Mix (NEB; M0494S) in two separate reactions to amplify the KK10 or the KY9 epitope ...
-
bioRxiv - Immunology 2020Quote: ... and amplified by PCR for 11 cycles using NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs). PCR products were purified using a PCR Purification Kit (Qiagen ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Inverse PCR was performed using NEBNext high-fidelity 2X PCR master mix (New England Biolabs, catalog no. M0541). DNA was purified with SPRISelect beads ...
-
bioRxiv - Genomics 2021Quote: ... we performed an initial PCR for locus-specific amplicon enrichment using NEBNext 2X High Fidelity PCR MM (NEB) and 5’-biotinylated stub adapter primers specific to appropriate genomic regions to be interrogated (Figure 5-table supplement 1) ...
-
bioRxiv - Immunology 2022Quote: ... and amplified by PCR for 11 cycles using NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs). PCR products were purified using a PCR Purification Kit (Qiagen ...
-
bioRxiv - Microbiology 2022Quote: ... PCR amplification of the dhodh locus was performed using Phusion High-Fidelity PCR Master Mix (New England BioLabs) as per the protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Tagmented DNA was amplified by PCR using 1x NEBNext Hi-Fidelity PCR Master Mix (New England Biolabs, NEB), using Nextera Index i5 and i7 series PCR primers ...
-
bioRxiv - Microbiology 2022Quote: ... Tagmented DNA was amplified by PCR using 1x NEBNext Hi-Fidelity PCR Master Mix (New England Biolabs, NEB), using Nextera Index i5 and i7 series PCR primers ...
-
bioRxiv - Molecular Biology 2022Quote: ... tagmented DNA was PCR amplified using 1x Phusion® High-Fidelity PCR Master Mix with GC Buffer (NEB) and 1.25 μM i5 and i7 PCR primers (Nextera® Index Kit (Illumina) ...
-
bioRxiv - Microbiology 2023Quote: ... PCR reactions were undertaken using sporocarp extractions using the PHusion High-Fidelity PCR Master Mix (New England Biolabs). Libraries were generated using the TruSeq DNA PCR-Free Prep Kit (Illumina ...
-
bioRxiv - Systems Biology 2023Quote: The pool of guide arrays was PCR amplified using NEBNext® High-Fidelity 2X PCR Master Mix (NEB) using 196 ng of starting template per 50 μl reaction using forward and reverse primers:
-
bioRxiv - Developmental Biology 2023Quote: ... and beads resuspended in a 50-µl PCR mix containing 1x NEBNext High-Fidelity PCR Master Mix (NEB) and 0.5 µM of Nextera Ad1_noMX and Ad2.X primers ...
-
bioRxiv - Cell Biology 2023Quote: ... A second PCR was performed using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs; #M0543) and Index 1 (i7 ...
-
bioRxiv - Developmental Biology 2023Quote: ... PCR was performed for 12 cycles using NEBnext High-Fidelity 2X PCR Master Mix (New England Biolabs, M0541L) (41) ...
-
bioRxiv - Developmental Biology 2021Quote: PCR-amplified amplicons (Hotstart Q5 polymerase, NEB) were sanger sequenced using Genewiz sanger sequence service under standard manufacture instructions.
-
bioRxiv - Molecular Biology 2021Quote: ... were subjected to a PCR test (NEB Taq Polymerase ...
-
bioRxiv - Molecular Biology 2019Quote: ... PCR was conducted with OneTaq® (NEB), per manufacture’s protocol ...
-
bioRxiv - Microbiology 2019Quote: ... PCR was run using Q5 polymerase (NEB) and a 50-62 °C annealing temperature gradient ...
-
bioRxiv - Developmental Biology 2020Quote: PCR products were digested with BsrI (NEB). Fragment sizes are 188 bp + 221 bp for wild type allele and 409 bp for vegfabmu155 allele.
-
bioRxiv - Developmental Biology 2020Quote: PCR products were digested with BccI (NEB). Fragment sizes are 116 bp + 123 bp for wild type allele and 239 bp for vegfaamu128 allele.
-
bioRxiv - Plant Biology 2019Quote: ... PCR reactions were performed with OneTaq (NEB), restriction enzymes were purchased from NEB as well as the T4 ligase and 100bp respectively 1kb ladder ...
-
bioRxiv - Genetics 2019Quote: ... PCR with OneTaq (NEB, Ipswich, MA, USA) was used to amplify wildtype Xenopus dyrk1a excluding the nucleotides coding for the stop codon ...
-
Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1)bioRxiv - Plant Biology 2021Quote: ... following a Phusion® PCR protocol (NEB). The PCR product and the binary plasmid pMDC43 were subsequently cut with the restriction enzymes HindIII-HF and KpnI-HF to remove the 2×35S promoter ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... PCR amplification followed the recommended protocol (NEB) with 12 PCR cycles and a 50 sec ...
-
bioRxiv - Biochemistry 2021Quote: ... Colony PCR (OneTaq polymerase, New England Biolabs) with the primer pairs clp2_KO_A–pLSV101_seq rev and pLSV101_seq fwd–clpP2_KO_D (see Tables 2 and 3 ...
-
bioRxiv - Microbiology 2020Quote: ... Colony PCRs used OneTaq DNA Polymerase (NEB). Restriction enzymes (NEB ...
-
bioRxiv - Developmental Biology 2022Quote: ... we used Q5 PCR mix (NEB, M0492L) to amplify the libraries for high-throughput sequencing in Illumina platform.
-
bioRxiv - Molecular Biology 2022Quote: ... PCR with taq DNA polymerase (NEB M0273S) followed by agarose gel electrophoresis was used to screen for the presence of the 3xFLAG tag and the YPWM-AAAA mutation ...
-
bioRxiv - Cancer Biology 2022Quote: ... PCR-amplified (NEBNext Ultra II Q5; NEB) using primers DCF01 5’-CTTGTGGAAAGGACGAAACACCG-3’ and DCR03 5’-CCTAGGAACAGCGGTTTAAAAAAGC-3’ and subjected to single-end 75 bp (SE75 ...
-
bioRxiv - Microbiology 2022Quote: ... using colony OneTaq PCR (New England Biolabs). For protease activity ...
-
bioRxiv - Synthetic Biology 2020Quote: ... PCR products were treated with DpnI (NEB) and purified using a gel extraction kit (Qiagen) ...
-
bioRxiv - Immunology 2021Quote: ... PCR amplicons were digested using PvuII (NEB) restriction enzyme at 37°C for 1 hour and were run on an agarose gel ...
-
bioRxiv - Developmental Biology 2022Quote: ... PCR products were amplified with Phusion (NEB), following the manufacturer’s specifications ...
-
bioRxiv - Biochemistry 2021Quote: ... Colony PCR (OneTaq polymerase, New England Biolabs) with the respective primer pairs clp_A–pLSV101_seq rev and pLSV101_seq fwd–clp_D (Table 2 ...
-
bioRxiv - Developmental Biology 2020Quote: ... PCR amplicons were column purified (NEB, T1030) and used as a template for in vitro transcription reactions with a mMESSAGE mMACHINE® T7 Transcription Kit (Ambion ...
-
bioRxiv - Microbiology 2021Quote: ... PCR fragments were gel purified (NEB T1020L) and cloned into pMiniT 2.0 vector (NEB E1202S) ...