Labshake search
Citations for New England Biolabs :
9351 - 9400 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... using the NEBNext® Ultra TM II DNA Library Prep Kit (NEB E7645S/L, New England BioLabs ® Inc., Ipswich, MA, USA) as described previously (6 ...
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bioRxiv - Cell Biology 2024Quote: ... Purified RNA was treated with PNK4 before being used for cDNA synthesis using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® Kit (NEB). Obtained cDNA template was amplified by PCR using the LongAmp® Taq 2XMaster Mix (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and the eluate was pooled together and used to construct DNA libraries for Illumina deep sequencing using an NEB DNA Library Prep Kit (NEB; cat # E7645S). The prepared DNA libraries were stored at –80°C before being sequenced at the Tufts University Core Facilities (US).
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bioRxiv - Microbiology 2024Quote: ... For Nanopore sequencing high-molecular weight DNA was isolated from a separately cultivated batch with the Monarch Genomic DNA Purification kit (New England Biolabs, Frankfurt, Germany) and library preparation was performed with 1.5 µg using the ligation sequencing kit 1D (SQK-NBD114.24 ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μg of the RNA were sent to Azenta Life Sciences for genome-wide mRNA sequencing using NEBNext Ultra RNA Library Prep Kit (New England Biolabs, Ipswich, MA) for NovaSeq (Illumina ...
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bioRxiv - Genetics 2024Quote: ... Samples with RIN ≥ 5.6 were used for sequencing library preparation using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and assembly of the pUC57-TS-ACC was performed by using NEBridge Golden Gate Assembly Kit (BsaI-HF v2) (New England Biolabs, Ipswich, MA). The assembled plasmid was verified by whole plasmid sequencing (Eurofins ...
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bioRxiv - Molecular Biology 2024Quote: ... CUT&RUN libraries have been generated using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB Cat #E7645S) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The total RNA of 6 samples were used for sequencing with UltraTM RNA Library Prep kit for Illumina® (NEB, MA, USA); all the standards and procedures were performed following the manufacturer’s protocols ...
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bioRxiv - Zoology 2024Quote: ... an Illumina library with an average insert size of 350 bp was constructed using NEB Next Ultra DNA Library Prep Kit (NEB, Beijing, China) with PE 150 bp mode.
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bioRxiv - Neuroscience 2024Quote: ... Between 72-92 ng of RNA per sample was subjected to rRNA depletion via the NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat; NEB #E7400) using the manufacturer’s protocol except that a Monarch® RNA Cleanup Kit (10 µg ...
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bioRxiv - Molecular Biology 2024Quote: ... The library prep was performed with the samples on beads using the NEBNext® Ultra II Directional DNA Library Prep Kit (NEB E7645), with several modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... halys adults were pooled in 400 µL of a DNA/RNA-protecting solution (Monarch Total RNA Miniprep Kit, New England Biolabs Inc., Germany) for each biological replicate ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA fragments (NheI-CMV-fp-tPT2A-fp-MfeI) containing MuSIC barcodes were isolated by the Monarch Gel DNA Extraction Kit (New England Biolabs, Cat# T1020L) and subject to end-prep ...
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bioRxiv - Genomics 2024Quote: ... Illumina libraries were then prepared using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs) and purified on NucleoSpin® Gel and PCR Clean-up columns (Takara Bio) ...
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bioRxiv - Genomics 2024Quote: ... The library was made according to the manufacturer’s directions for the NEBNext Enzymatic Methyl-seq kit (New England Biolabs, Cat. No. E7120S) using the S220 Focused-ultrasonicator (Covaris ...
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bioRxiv - Genomics 2024Quote: Library preparation was conducted using a protocol modified from the manufacturer’s instructions included in the NEBNext® Library Preparation Kit for 454 (E6070S, New England Biolabs, Ipswich, MA) as detailed in 68 except that libraries were single indexed and not split into two amplifications for PCR ...
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bioRxiv - Genomics 2024Quote: ... The PE RNA sequencing libraries were prepared using the NEBNEXT Ultra RNA Library Prep Kit for Illumina (New England Biolabs Inc.,USA) and 150 bp PE sequencing was performed on Illumina Hiseq X Ten platform ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... RNAseq libraries were made from the enriched mRNA using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina per the manufacturer’s instructions (New England Biolabs, Massachusetts, USA). After library preparation ...
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bioRxiv - Genetics 2024Quote: DNA from mNPCs in culture were harvested from Kmt2a+/LSL cell line cell line (1 million cells) using Monarch® HMW DNA Extraction Kit (NEB, T3050). We sheared the high molecular weight (HMW ...
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bioRxiv - Genomics 2024Quote: ... RNA-seq libraries were prepared using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Cat# E7760) according to manufacturer protocol.
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bioRxiv - Synthetic Biology 2024Quote: ... Each probe was ligated with native barcodes from the nanopore native barcoding kit using the blunt/TA ligase master mix (New England Biolabs, Cat# M0367S). The ligation products from individual fluorescent probes were pooled and underwent a second round of purification using the AMPure XP Beads ...
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bioRxiv - Synthetic Biology 2024Quote: ... The pooled probes were further ligated with the adapters from the native barcoding kit using the NEB quick T4 ligase (New England Biolabs, Cat# E6056S). After a third round of purification and quantification ...
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bioRxiv - Bioengineering 2024Quote: ... The RNA samples were quantified and used as templates for one-step RT-qPCR using Luna Universal One-Step RT-qPCR kit (NEB, MA, USA). Real time PCR amplification was performed using CFX96 touch real-time PCR detection system (Biorad ...
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bioRxiv - Biophysics 2024Quote: ... The PDZ and Protease domain of HtrA1 were amplified using primers 5’-ATCACCAAGAAGAAGTATATTG-3’ and 5’-GGATCCTTTTTCGAACTGC-3’ as well as 5’-TAGCTCGAGCACCACCAC-3’ and 5’-TTTGGCCTGTCGGTCATG-3’with a NEB Q5® Site-Directed Mutagenesis Kit (New England Biolabs, MA). The mutation of S328A of HtrA1 and its Protease domain were created using primers 5’-CTATGGAAACgcgGGAGGCCCGT-3’ and 5’-TTGATGATGGCGTCGGTCTG-3’ with a NEB Q5® Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Biophysics 2024Quote: ... The mutation of S328A of HtrA1 and its Protease domain were created using primers 5’-CTATGGAAACgcgGGAGGCCCGT-3’ and 5’-TTGATGATGGCGTCGGTCTG-3’ with a NEB Q5® Site-Directed Mutagenesis Kit (New England Biolabs, MA). The PCR protocol was as followed ...
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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activitybioRxiv - Bioengineering 2024Quote: ... Purified DNA was submitted to the University of Wisconsin-Madison Biotechnology Center DNA Sequencing Facility and a sequencing library was prepared using the NEBNext Ultra II kit (New England Biolabs, Beverly, MA). Samples were sequenced using an Illumina NovaSeq6000 (Illumina ...
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bioRxiv - Bioengineering 2024Quote: ... Illumina adapters were ligated to the resultant fragments and barcoded by PCR with primers from the NEBnext multiple oligos for the Illumina kit (New England Biolabs, cat. # E7600) and Phusion DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... G4 elements (listed in Supplementary Table S1) were inserted into BPCU by site-directed mutagenesis using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, USA). Plasmid templates for in vitro transcription reactions were generated by cloning appropriate RNA G4-eGFP sequences into pRNA128A (37 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The base plasmid was digested with BmtI and XbaI (Library 1) or BsaI (Library 2) and purified on a 1% agarose gel using Monarch® DNA Gel Extraction Kit (NEB, T1020L). We used a Gibson Assembly reaction (NEBuilder® HiFi DNA Assembly Master Mix ...
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bioRxiv - Cell Biology 2024Quote: For each sample 2 ml of log phase culture were harvested and total RNA extraction was performed using Monarch® Total RNA Miniprep Kit (New England Biolabs T2010S) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... were introduced into a pENTR1A 1xFLAG-tag (FT) WT FBLN3 DNA template (described previously (24)) using the Q5 Site-Directed Mutagenesis kit (New England Biolabs, Ipswich, ME). pENTRIA constructs were verified by Sanger sequencing before being introduced into the pcDNA DEST40 vector (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA underwent polyA selection and RNA sequencing libraries preparation using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... Point mutations were introduced to the pBApo-GALC-VH construct with the Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA). Non-overlapping primer pairs for each MM are listed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... was purchased from GeneCopia (EX-OL00093-LX304).The C-terminal 11 amino acids were deleted using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, E0554S). Primers were designed using the NEBase Changer tool to generate the deletion were ...
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bioRxiv - Genetics 2024Quote: ... 50 µl of sheared DNA was used for enzymatic methyl conversion following the NEBNext® Enzymatic Methyl-seq Kit (NEB #E7120S/L) for standard libraries using the formamide denaturation protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RNA poly-A selected sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... The appropriate size band was cut out of the gel and cleaned using the Monarch DNA Gel Extraction Kit Protocol (NEB Cat #T1020). For deep sequencing ...
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bioRxiv - Genomics 2024Quote: ... Libraries were prepared according to manufacturer’s instructions using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs; Cat. No. E7760). Depending on the RIN value of RNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were prepared for sequencing using the NEB Ultra II DNA Library Prep kit for Illumina library preparation (New England Biolabs, Ipswich, Massachusetts). Samples were combined by mass and prepared for sequencing following the MiSeq System Denature and Dilute Libraries Guide (Document # 15039740 v10 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and constructed a sequencing library for each individual using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Massachusetts, USA). For the Aripo dataset ...
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bioRxiv - Evolutionary Biology 2024Quote: ... using a protocol with 10% of the reagents provided in the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, Ipswich, MA). Steps for all library preparations are described in more detail in [11] ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Paired-end libraries for generation F60 were prepared and sequenced along with those for generation F0 using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, MA) with an insert size of 300bp ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The final library was prepared with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645, #E7103), and sequencing was carried out on the Illumina HiSeq 2500 platform.
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bioRxiv - Evolutionary Biology 2024Quote: ... followed by library preparation using the NEBNext UltraTM II FS DNA Library Preparation Kit for Illumina (New England Biolabs, Ipswich, MA, USA). Then ...
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bioRxiv - Cell Biology 2024Quote: ... libraries were constructed from 1µg of total RNA treated with DNaseI using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... 500 ng to 1 µg of total RNA was converted to Illumina sequencing libraries using the NEB Ultra II Directional RNA Library Prep kit with Sample Purification Beads (NEB cat. #E7765), with 10-14 PCR cycles (determined empirically to achieve the minimum number of cycles per library) ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 μg of total RNA was used to purify mRNA using NEBNext Poly(A) mRNA Magnetic Isolation Kit (E7490, New England Biolabs, Massachusetts, USA). RNA-seq libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit (E7420L ...
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bioRxiv - Microbiology 2020Quote: ... About 200ng of DNA was taken and preceded for library preparation using NEBNext Ultra II DNA kit (E7645, New England Biolabs, Massachusetts, United States) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... prior to library preparation with NeBNext®Ultra™II Directional RNA Library Prep Kit for Illumina (both New England BioLabs, Ipswich, USA) using an RNA input of 100 ng per sample (New England BioLabs ...