Labshake search
Citations for Illumina :
7901 - 7950 of 8781 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... ~800 ng of total RNA was depleted of rRNA using the Ribo-Zero rRNA Removal Kit (Illumina, Inc., San Diego, CA, USA), sheared ...
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bioRxiv - Genetics 2020Quote: ... RNA fragments were reverse-transcribed to create the final cDNA libraries following the NEBNext® Ultra™ RNA Library Prep Kit (Illumina) and paired-end sequencing 150bp was performed on a Novaseq 6000 ...
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bioRxiv - Genetics 2020Quote: ... A genome-wide analysis genotyping scan was performed in all six members of family A and in the proband of family B using the HumanCytoSNP-12 DNA Analysis BeadChip Kit (Illumina, San Diego), according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were prepared using the TruSeq Stranded Total RNA prep kit with Ribo-Zero Gold to remove cytoplasmic and mitochondrial rRNA according to the manufacturer’s recommendation (Illumina, San Diego, CA). Total RNA-seq libraries were run on an Illumina NextSeq 500 sequencing instrument according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were sequenced using a 150 bp paired-end NextSeq High Output V2 reagent kit (Illumina, FC-404-2004, San Diego, CA). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... PolyA+ selected libraries were prepared with the TruSeq mRNA Stranded Library Prep Kit with TruSeq UDI96 indexed adaptors (Illumina, San Diego, CA). Samples were multiplexed and sequenced on the Illumina NovaSeq 6000 S4 to produce approximately 100 million ...
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bioRxiv - Genetics 2022Quote: ... to approximately 450 bp inserts followed by library preparation using the Seqlab TruSeq Nano DNA HT kit (20000903, Illumina, San Diego, USA). Library preparation was performed according to manufacturer’s instructions which included end repair ...
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bioRxiv - Genetics 2022Quote: ... Approximately 1 µg of total RNA were used to prepare the small RNA library according to the protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego). Then the single-end sequencing 50bp was performed on an Illumina Hiseq 2500 at LC Sciences (Hangzhou ...
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bioRxiv - Cancer Biology 2022Quote: ... using MiSeq V2 300 cycle reagent kit after the amplified library was purified with AMPure XP beads (Beckman Coulter, CA, USA) (Illumina, CA, USA) (10).
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bioRxiv - Bioengineering 2022Quote: ... the organoid was then resuspended in a direct permeabilization/tagmentation mastermix (25μl TD buffer (from Illumina Nextera XT DNA Library Prep Kit), 2.5μl Tn5 ...
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bioRxiv - Cancer Biology 2022Quote: ... The libraries were prepared from total RNA by using the TruSeq Stranded mRNA Library Prep kit according to the manufacturer’s instructions (Illumina, San Diego, CA). The libraries were analyzed for insert size distribution by using a 2100 BioAnalyzer High Sensitivity kit (Agilent) ...
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bioRxiv - Genomics 2022Quote: ... The construction of libraries was performed with Illumina preparation kits (Table S1) following the manufacturer’s recommendations (Illumina Inc., San Diego, CA, USA). The Nextera DNA Prep kit and Illumina DNA Prep were used for WGS ...
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bioRxiv - Plant Biology 2022Quote: ... RNA-seq libraries were constructed by the POPS platform (IPS2) using the TruSeq Stranded mRNA library prep kit (Illumina®, California, U.S.A.) according to the supplier’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... DNA libraries were prepared according to the manufacturer’s protocol using the Nextera DNA Flex Library Preparation Kit (Illumina, San Diego, CA, USA). Library preparation and bead clean-up was undertaken with the Mantis® Liquid Handler (Formulatrix ...
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bioRxiv - Microbiology 2022Quote: ... The same amount of the seven purified 3-cycle PCR products were further amplified using Platinum SuperFi II and the index primers from Nextera XT Index Kit v2 (Illumina cat. 15052163). The PCR products were purified using 1.8× beads ...
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bioRxiv - Genomics 2022Quote: Genomic libraries were prepared from 1 ng of high-quality genomic DNA using the Nextera DNA Flex Library Prep kit (now called Illumina DNA Prep, Illumina C.A., USA), following the manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... Amplified (1 ng) cDNA was used to generate barcoded libraries using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA). The size of the libraries was checked using the Agilent 2100 Bioanalyzer High Sensitivity DNA chip and their concentration was determined using the Qubit® fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: We prepared cDNA sequencing libraries for the transcriptome samples using the TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA, USA). Paired-end fragment reads were generated on an Illumina NextSeq sequencer using TG NextSeq® 500/550 High Output Kit v2 (300 cycles) ...
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bioRxiv - Synthetic Biology 2022Quote: ... pH 8.5 and sequenced on an Illumina NextSeq 550 following the manufacturer’s instructions using a NextSeq 500/550 Mid or High Output Kit (Illumina, 20024904 or 20024907), or on an Illumina NextSeq 1000 following the manufacturer’s instructions using NextSeq P2 v3 kits (Illumina ...
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bioRxiv - Plant Biology 2022Quote: ... Five micrograms of total RNA from each sample were used to construct an RNA-Seq library by using the TruSeq RNA Sample Preparation Kit according to the manufacturer’s instructions (Illumina, San Diego, CA). A total of 72 libraries (Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 000 nuclei were subjected to Tn5 transposition reaction using 2.5 μl TDE1 from Nextera DNA Library Prep Kit (Illumina, #FC-121-1030). After adding the transposition reaction mix ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNAs from WT and e2fabc at 9 DAG were used for construction of cDNA libraries using the TruSeq RNA Library Preparation Kit v2 (Illumina, United States) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of total RNA was used as input for the TruSeq RNA Library Prep Kit v2 (Illumina, Inc., San Diego, CA) and the manufacturer’s protocol was followed ...
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bioRxiv - Microbiology 2022Quote: ... Amplified cDNA was fragmented and appended with dual-indexed barcodes using Illumina Nextera XT DNA Library Prep kits (Illumina, San Diego, CA). Libraries were validated on an Agilent 4200 TapeStation (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-read sequencing was performed on a NovaSeq 6000 device using an S2 Reagent kit (100 cycles) according to the manufacturer’s instructions (Illumina Inc., CA, USA).
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bioRxiv - Microbiology 2022Quote: ... enteroids were suspended in RNALater and RNA was isolated using Tru-Seq total RNA Sample Preparation v2 kit (Illumina, Great Abingdon, UK) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Complementary DNA libraries from total RNA samples were prepared with Illumina TruSeq RNA preparation kits according to the manufacturer’s instructions (Illumina, San Diego, CA). RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Santa Clara ...
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bioRxiv - Neuroscience 2022Quote: ... Libraries for RNA-seq were prepared using the paired-end TruSeq® Stranded mRNA Sample Preparation kit (Illumina, San Diego, Ca, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 150 pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... following the manufacturer’s instructions and libraries for shotgun metagenomic sequencing were prepared using Illumina DNA Prep kits (Illumina, San Diego, CA, USA). Briefly ...
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bioRxiv - Systems Biology 2024Quote: ... Libraries were then pooled and sequenced with a NextSeq 500/550 Mid Output v2 kit (150 cycles) according to the manufacturer’s recommendation (Illumina, FC-404-2001). Pooled samples were run in triplicate for a minimum sequencing depth of 20 million reads per sample ...
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bioRxiv - Systems Biology 2024Quote: ... the RNA was reverse-transcribed into Illumina shotgun sequencing libraries with TruSeq RNA stranded kit and polyA enrichment following the manufacturer’s protocol (Illumina, San Diego, CA) at the Competence Centre for Genomic Analysis (Kiel ...
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bioRxiv - Cancer Biology 2023Quote: ... 4) tagmentation was performed with 2ng input and sequencing library generated using the Nextera XT library prep kit (Illumina, #FC-131-1024). In short ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation for VLP genomic DNA involved using >25ng input DNA with the Nextera DNA Flex Library Preparation Kit (currently named Illumina DNA prep, Illumina, CA, USA). Deviating from the Illumina protocol’s full reaction volume (50 µL) ...
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bioRxiv - Physiology 2024Quote: ... Sequencing libraries were prepared with Watchmaker DNA Library Prep kits with Fragmentation (Watchmaker, CO, USA) and sequenced using the Illumina NovaSeq instrument (Illumina, CA, USA) with the shotgun sequencing method (a depth of 2 million 2x150 bp read pairs) ...
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bioRxiv - Physiology 2024Quote: ... Based RNA integrity value above 7 determined by the spectrofluorometric measurement RNA samples was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595; Illumina, San Diego, CA). Library preparation was done for each sample fitted with one of 96 adapters with different 8 base molecular barcode for high level multiplexing and following 15 cycles of PCR amplification ...
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bioRxiv - Evolutionary Biology 2023Quote: ... insert size 550 nt) were constructed from 1.8-2 µg gDNA with the TruSeq DNA PCR-Free kit (Illumina, San Diego, CA) and sequenced on the Illumina HiSeq 2500 by the Genomics Core Facility at Pennsylvania State University ...
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bioRxiv - Microbiology 2024Quote: The Illumina TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero Human/Mouse/Rat protocol (Illumina, Inc. San Diego, CA, USA) was used for the following steps ...
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bioRxiv - Neuroscience 2024Quote: ... RNAseq libraries were prepared from 2500 ng total RNA using the TruSeq RNA Library Preparation Kit v2 (Illumina, San Diego, CA, USA) after poly(A ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were prepared using 200 ng of total RNA according to the manufacturer’s instructions for the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries were determined using Qubit fluorometry and an Agilent BioAnalyzer DNA 1000 chip (Santa Clara ...
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bioRxiv - Genomics 2024Quote: ... were used as the input material (approximately 1 ng total quantity) for preparing shotgun metagenome sequencing library with the help of Nextera XT DNA Library Prep Kit (Illumina Inc., USA). Quality of the final library was assesed using high sensitivity D1000 screen tape in 2200 TapeStation (Agilent Technologies Inc. ...
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bioRxiv - Neuroscience 2024Quote: The Total RNA Library was prepared using the Illumina NovaSeq (S4) 300 cycle flow cell and reagent kit (Illumina, San Diego, CA). The high-throughput sequencing strategy included 300 cycles and a read length of paired-end 150 base pairs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were made according to the manufacturer’s directions for the TruSeq Small RNA Library Prep Kit (Illumina, Cat. No. RS-200-0012). Resulting libraries were size selected for 145-160bp fragments using Novex 6% TBE gels (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... Libraries for sequencing were prepared according to the manufacturer’s instructions with TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina, RS-122-2301), and paired-end sequencing was done with a NovaSeq 6000 instrument (Illumina) ...
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bioRxiv - Developmental Biology 2024Quote: ... The DNA extracted from these samples was used for subsequent library construction using the TruSeq Nano DNA LT Library Preparation Kit (Illumina Inc., USA) whose purity and size were validated using an Agilent Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: ... it enables the observation of all variable sequence elements (spacers, iBARs, and the middle tRNA) with 150-cycle sequencing kits (e.g. Illumina MS-102-3001) and positions spacers and iBARs as early as possible within NGS reads ...
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bioRxiv - Genomics 2023Quote: HMW DNA from 1.5 x 106 cells was used to generate Illumina libraries with the Nextera XT DNA Library Preparation kit (Illumina FC-131-1024) and Illumina DNA PCR-Free Library Prep ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 1 ng/μl and subjected to library preparation using Nextera XT DNA Library Preparation Kit (Illumina, CA, USA) according to the manufacturer’s instructions and sequenced with NextSeq550 using Mid Output Kit v2.5 (300 cycles ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing was performed on an Illumina NovaSeq 6000 instrument using the NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles) (Illumina, cat. no. 20028317), in a pair-end 2x100 cycle mode ...
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bioRxiv - Immunology 2024Quote: Library preparation was performed starting from 200ng total RNA of each sample using the TruSeq Stranded mRNA Library Kit (Illumina, cat. # 20020595) and the TruSeq RNA UD Indexes (Illumina ...