Labshake search
Citations for Illumina :
7851 - 7900 of 8781 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... RNA libraries were prepared using approximately 100 ng of total RNA according to the manufacturer’s instructions for the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA), employing poly-A mRNA enrichment using oligo dT magnetic beads ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were diluted and 150 pg input cDNA was used with the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA) to generate indexed libraries ...
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bioRxiv - Immunology 2020Quote: RNA libraries were prepared using 100 ng of good quality total RNA according to the manufacturer’s instructions for the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA) employing poly-A mRNA enrichment using oligo dT magnetic beads ...
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bioRxiv - Microbiology 2021Quote: ... metatranscriptomic libraries were prepared directly from total RNA without rRNA removal using the TruSeq RNA Kit (Illumina, Inc., San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... commercial reagents were used for unique dual indexed amplicon library generation according to the Artic protocol (NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®)) ...
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bioRxiv - Neuroscience 2021Quote: The method used to amplify RNA was adapted from Abruzzi et al (Abruzzi et al., 2015) and was performed using TruSeq® Small RNA Library Prep kit (Illumina, USA) and following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Samples were normalized to 1.67 × 105 16S rRNA gene copies/μL prior to barcoding and Illumina MiSeq sequencing (reagent kit v3; Illumina, San Diego, CA) at the University of Minnesota Genomics Center (Minneapolis ...
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bioRxiv - Microbiology 2021Quote: ... The libraries were sequenced using an Illumina MiSeq with MiSeq Reagent Kit v.3 (2x 300 bp, Illumina, San Diego, CA, USA) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The final pool was sequenced on Illumina MiSeq with a 2×250bp PE Illumina Reagent Kit v2 (Illumina, Inc., San Diego, CA) in the Biotechnology Center at the University of Wisconsin-Madison
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bioRxiv - Immunology 2020Quote: ... and ligation of the Illumina indexed adapters were performed according to the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries was determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara ...
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bioRxiv - Immunology 2020Quote: ... and ligation of the Illumina indexed adapters were performed according to the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries was determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara ...
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bioRxiv - Microbiology 2021Quote: ... A single library from these samples (Acerv FL 14120) was prepared using the TruSeq DNA PCR-Free kit (Illumina, San Diego, CA) and the remaining samples were prepared using the TruSeq DNA Nano kit (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: Strand-specific libraries were generated using the TruSeq Stranded mRNA samples preparation kit (illumine Inc., San Diego, RS-122-2101/2) according to manufacturer’s instructions (Illumina, part #15031047 Rev.E). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... Library preparation and RNA-sequencing was performed at the Lausanne Genomic Technologies Facility (LGTF, University of Lausanne; TruSeq Stranded Total RNA Library Kit; Illumina HiSeq 2500). The samples were barcoded and randomized between lanes for sequencing ...
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bioRxiv - Immunology 2022Quote: ... libraries for sequencing were prepared using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® following the manufacturer’s instructions (Illumina). Quality of all RNA and library preparations were evaluated with BioAnalyser 2100 (Agilent) ...
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bioRxiv - Immunology 2020Quote: ... 0.1 ng of cDNA from each cell on the plate was individually tagmented using the Nextera XT kit (Illumina, San Diego, CA) in a final volume of 5 µl ...
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bioRxiv - Genomics 2020Quote: ... 2 µl each of P7 and P5 Nextera XT Index Kit v2 index primers (Illumina Catalogue numbers FC-131-2001 to 2004) were added to each well ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was diluted 1:15 and subsequently tagmented and amplified for 12 cycles using the Nextera XT DNA Library Preparation Kit (Illumina FC-131). Individual libraries were QC’d by Qubit and Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... pooled and then purified using AMPure beads before running index-PCR using the Nextera XT Index Kit (Illumina, San Diego, CA, USA). AmpliSeq libraries were subsequently normalized before sequencing the libraries as 300bp paired-end reads on Illumina MiSeq (Illumina ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was diluted to approximately 2.5 ng/μL and enzymatically sheared using the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina, San Diego, CA) at 55°C for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... The construction of the RNA library was performed according to the manufacturer’s protocol using the TruSeq RNA Library Preparation Kit (TruSeq RNA Sample Prep v2, Illumina, San Diego, CA). All libraries were sequenced using Illumina HiSeq 2000 platform with 151 bp paired-end strategy ...
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bioRxiv - Physiology 2021Quote: ... Libraries for sequencing of bone marrow and spinal cord were prepared with the TruSeq stranded mRNA kit and sequenced with read length SR50 (Illumina HiSeq 4000). For all other tissues RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina ...
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bioRxiv - Microbiology 2020Quote: ... the clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v4-cBot-HS (Illumina, Inc. USA) according to the manufacturer’s instructions and the library preparations were sequenced on an Illumina Hiseq 2500 platform and paired-end reads were generated.
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bioRxiv - Microbiology 2020Quote: ... sequencing libraries were prepared using the Nextera XT DNA library preparation kit according to the manufacturer’s instructions (Illumina, Inc., San Diego, CA). PhiX DNA (15% [vol/vol] ...
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bioRxiv - Immunology 2020Quote: ... and ligation of the Illumina indexed adapters were performed according to the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries was determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We used 200 ng of DNA from each sample to prepare individually barcoded libraries with a 550bp insert size following the protocol for the TruSeq Nano DNA Library Prep kit (Illumina, California, USA). The libraries were pooled into three groups and sequenced separately on an Illumina NextSeq500 lane (2×150bp ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were constructed using Nextera® XT DNA Library Preparation Kits and 96 well v2 indexes (Illumina, San Diego, CA, USA). The libraries were quality checked using an Agilent 4200 TapeStation and pooled at equimolar concentrations ...
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bioRxiv - Genetics 2020Quote: ... One sequencing experiment (Experiment 1) was performed according to the manufacturer’s directions for the TruSeq Small RNA Sample Preparation Kit (Illumina, RS-200-0012). The protocol was adapted to incorporate a 2S blocking DNA oligo for removal of prevalent Drosophila small ribosomal RNA from the sequencing library [67] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA sequencing libraries were prepared from the same material using the Illumina TruSeq stranded RNA-seq preparation kit (Illumina Inc., United States) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Genome libraries for short-read sequencing were prepared with the TruSeq DNA PCR-Free Sample Prep Kit (Illumina, San Diego, CA, USA), and sequenced on the NextSeq 500 platform (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Illumina sequencing adapters and dual-index barcodes were added to the purified amplicon products using limited cycle PCR and the Nextera XT Index Kit (Illumina, CA, USA). Amplicons from 96 samples and controls were pooled in equimolar amounts and the resultant libraries were purified by gel extraction and quantified (Qiagen ...
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Enzymes degraded under high light maintain proteostasis by transcriptional regulation in ArabidopsisbioRxiv - Plant Biology 2021Quote: ... Total RNA-sequencing libraries were prepared using the TruSeq Stranded Total RNA with Ribo-Zero Plant kit (RS-122-2402, Illumina, CA, USA) as per manufacturer’s instructions but with input RNA and reaction volumes adjusted by one-third ...
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bioRxiv - Genetics 2021Quote: ... 300 ∼ 420 ng of RNA was used for E8.5 cDNA library generation with the KAPA-Stranded mRNA-seq kit (Illumina Platforms, KR0960-v5.17).
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bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted from five biological replicates of each of the 222 and ii2 diet groups (10 samples in total) using the TruSeq Stranded mRNA-seq kit (Illumina, DA, USA). The samples were sequenced in one flowcell on the NextSeq 500 producing paired end reads of 75bp.
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bioRxiv - Microbiology 2021Quote: Resistome enriched libraries and unenriched metagenomic libraries were sequenced on an Illumina HiSeq X Ten using a HiSeq X Ten Reagent Kit v2.5 (300 cycles) (Illumina, San Diego, CA) to obtain PE150 reads ...
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bioRxiv - Cancer Biology 2021Quote: ... Strand-specific libraries were generated using the TruSeq Stranded mRNA samples preparation kit (illumine Inc., San Diego, RS-122-2101/2) according to manufacturer’s instructions (Illumina, part #15031047 Rev.E). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were constructed using a QuantSeq 3’mRNA-Seq Library Prep Kit (LEXOGEN, Vienna, Austria) and sequenced with the NextSeq500 (Illumina, CA, USA) to generate a minimum of two million single-end 75-bp reads ...
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bioRxiv - Microbiology 2021Quote: ... 2 µL each of P7 and P5 of Nextera XT Index Kit v2 index primers (catalogue No. FC-131-2001 to 2004; Illumina, Cambridge, UK) were also added to each well ...
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bioRxiv - Genomics 2020Quote: ... The cDNA library was generated from 1 µg of total RNA using TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA) following manufacturer’s protocols ...
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bioRxiv - Genetics 2021Quote: ... Next generation sequencing library preparations were constructed following the manufacturer’s protocol (NEBNext® Ultra™ DNA Library Prep Kit for Illumina®). For each sample ...
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bioRxiv - Genomics 2021Quote: Next generation sequencing library preparations were constructed following the manufacturer’s protocol (NEBNext® Ultra™ DNA Library Prep Kit for Illumina®). The libraries were sequenced using the Illumina HiSeq instrument with PE150 configuration by Genewiz Co. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and cDNA was synthesized using random hexamers and reverse transcriptase (included in the NEB Next® Ultra™ RNA Library Prep Kit for Illumina). After the first-strand synthesis ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Illumina sequencing libraries were generated for 5 αHHβHH mice and 5 αLLβHL mice using TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA, USA) and sequenced on an Illumina HiSeq2500 platform ...
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bioRxiv - Microbiology 2021Quote: Indexing of purified PCR products was performed using the Nextera XT Index kit v2 (Set D) as per manufacturer’s instruction (Illumina Inc, CA, USA). The libraries after indexing were quantified using Qubit dsDNA BR assay kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The Illumina RNA-seq cDNA library was prepared using 1 μg of RNA from each sample and TruSeq V2 kits (Illumina, CA, USA), with reduced RNA fragmentation time (3 minutes ...
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bioRxiv - Immunology 2020Quote: ... One microgram of high quality RNA (RIN>8) was used as a template for library generation using the Illumina TruSeq RNA Sample Prep Kit v2 (Illumina; CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries for RNA-Seq were prepared from 1 µg of total RNAs with the TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA), multiplexed and sequenced in two runs with an Illumina NextSeq500 device (high throughput mode ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were diluted to a final concentration of 2.8 pM in HT1 buffer (supplemented with the kit) and loaded on 75-cycle high-output flow cells (Illumina, FC-404-2005) and sequenced on a NextSeq 550 (Illumina) ...
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bioRxiv - Cell Biology 2021Quote: ... was prepared into barcoded sequencing libraries using the TruSeq Stranded Total RNA kit with Ribo-Zero Plus rRNA Depletion (Illumina, Cambridge, UK) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Libraries with 250-350 bp insert sizes were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA). To obtain transcriptome profiles ...