Labshake search
Citations for Illumina :
7951 - 8000 of 8781 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 1 ng/μl and subjected to library preparation using Nextera XT DNA Library Preparation Kit (Illumina, CA, USA) according to the manufacturer’s instructions and sequenced with NextSeq550 using Mid Output Kit v2.5 (300 cycles ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing was performed on an Illumina NovaSeq 6000 instrument using the NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles) (Illumina, cat. no. 20028317), in a pair-end 2x100 cycle mode ...
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bioRxiv - Immunology 2024Quote: Library preparation was performed starting from 200ng total RNA of each sample using the TruSeq Stranded mRNA Library Kit (Illumina, cat. # 20020595) and the TruSeq RNA UD Indexes (Illumina ...
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bioRxiv - Microbiology 2024Quote: The Illumina TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero Human/Mouse/Rat protocol (Illumina, Inc. San Diego, CA, USA) was used for the following steps ...
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bioRxiv - Developmental Biology 2023Quote: ... Complementary DNA libraries from total RNA samples were prepared with Illumina TruSeq RNA preparation kits according to the manufacturer’s instructions (Illumina, San Diego, CA). RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Santa Clara ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries for transcriptome analysis (RNA-Seq) were constructed using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... A library was prepared with 100 ng of total RNA for each sample using the TruSeq RNA Access library prep kit (Illumina, CA, USA). Subsequently ...
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bioRxiv - Developmental Biology 2023Quote: ... Approximately 300 ng of RNA was used for library prep (NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina) following standard kit procedure ...
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bioRxiv - Cell Biology 2023Quote: RNA libraries were prepared using 200 ng of total RNA in 20 µl according to the manufacturer’s instructions for the Illumina Stranded mRNA Ligation Sample Prep Kit (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries were determined using an Agilent Bioanalyzer DNA 1000 chip and Qubit fluorometry (Invitrogen) ...
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bioRxiv - Physiology 2023Quote: ... high-sensitivity spectrofluorometric measurement was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595; Illumina, San Diego, CA). Each sample was fitted with one of 96 adapters containing a different 8 base molecular barcode for high level multiplexing ...
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bioRxiv - Plant Biology 2023Quote: ... PolyA-enriched mRNA libraries were prepared from three biological replicates of dark and light samples using the TruSeqTM RNA Sample Preparation Kits (v2) and sequenced as 51-bp single-end reads using the Illumina HiSeq 2000 instrument (Illumina Inc., USA) according to the manufacturer’s protocol at the Center for Genomic Research and Biocomputing ...
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bioRxiv - Systems Biology 2024Quote: ... the RNA was reverse-transcribed into Illumina shotgun sequencing libraries with TruSeq RNA stranded kit and polyA enrichment following the manufacturer’s protocol (Illumina, San Diego, CA) at the Competence Centre for Genomic Analysis (Kiel ...
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bioRxiv - Systems Biology 2024Quote: ... Libraries were then pooled and sequenced with a NextSeq 500/550 Mid Output v2 kit (150 cycles) according to the manufacturer’s recommendation (Illumina, FC-404-2001). Pooled samples were run in triplicate for a minimum sequencing depth of 20 million reads per sample ...
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bioRxiv - Physiology 2024Quote: ... Sequencing libraries were prepared with Watchmaker DNA Library Prep kits with Fragmentation (Watchmaker, CO, USA) and sequenced using the Illumina NovaSeq instrument (Illumina, CA, USA) with the shotgun sequencing method (a depth of 2 million 2x150 bp read pairs) ...
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bioRxiv - Physiology 2024Quote: ... Based RNA integrity value above 7 determined by the spectrofluorometric measurement RNA samples was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595; Illumina, San Diego, CA). Library preparation was done for each sample fitted with one of 96 adapters with different 8 base molecular barcode for high level multiplexing and following 15 cycles of PCR amplification ...
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bioRxiv - Microbiology 2023Quote: ... The NGS library was prepared with the Nextera XT DNA Library prep kit following manufacturer’s instructions with changes (Illumina #FC-131-1096). Initially ...
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bioRxiv - Microbiology 2023Quote: ... The Seqwell plexWell LP384 Library Preparation Kit and Native Barcoding Kit 24 V14 (SQK-NBD112.24) were used for barcode-indexed whole genome sequencing with Illumina NovaSeq system (Illumina San Diego, USA) and Oxford Nanopore MinIoN Mk1C device (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Resultant genomic DNA was treated with RNase A and prepared for sequencing using the Nextera XT kit (Illumina Corp., San Diego, CA). Libraries were uniquely dual-indexed and pooled ...
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bioRxiv - Genomics 2023Quote: ... RNA libraries were created and individually barcoded from 2.7 µg of template total RNA utilizing the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) as described in the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the KAPA RNA HyperPrep Kit and sequenced on an Illumina HiSeq® 4000 sequencer (Illumina, San Diego, CA) at a minimum of 25 million paired end reads (75bp ...
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bioRxiv - Immunology 2023Quote: ... 150 picograms of cDNA was used to make sequencing libraries by Nextera XT DNA Sample Preparation Kit (Illumina catalog#: FC-131-1024). Libraries were quantified with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific catalog# ...
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bioRxiv - Cancer Biology 2023Quote: ... The libraries were sequenced with 54 paired-end reads on a NovaSeq-6000 using a Reagent Kit v1.5 (Illumina Inc., San Diego). For CD45+ RNA-seq and the cell line RNA-seq ...
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bioRxiv - Microbiology 2023Quote: ... Pooled cDNA libraries were sequenced paired end using an Illumina NovaSeq 6000 SP Reagent Kit v1.5 (100 cycles Illumina, San Diego, US) on an Illumina NovaSeq 6000 instrument ...
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing was done using Illumina NextSeq 500 sequencer using the Nextseq High Output V2 150 sequencing kit (Illumina, 150 bp, single reads). RNAseq bulk sequencing data (from MARS-seq ...
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bioRxiv - Microbiology 2023Quote: ... Whole-genome shotgun sequencing was carried out on an Illumina MiSeq platform using Nextera DNA Flex kits (Illumina, San Diego, CA, USA) with a 150-bp paired-end sequencing kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation for VLP genomic DNA involved using >25ng input DNA with the Nextera DNA Flex Library Preparation Kit (currently named Illumina DNA prep, Illumina, CA, USA). Deviating from the Illumina protocol’s full reaction volume (50 µL) ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing was performed on an Illumina NovaSeq 6000 platform with NovaSeq 6000 SP Reagent Kit v1.5 100 cycles (Illumina, cat. no. 20028401). RNA seq experiments were conducted with three independent biological replicates.
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bioRxiv - Microbiology 2023Quote: ... A second 4 nM pool was generated and sequenced on the Illumina NovaSeq 6000 with the NovaSeq 6000 S4 Reagent v1.5 (300 cycles) kit (20028312, Illumina, San Diego, CA) at the Molecular Biology Core Facilities at the Dana-Farber Cancer Institute (Boston ...
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bioRxiv - Cancer Biology 2023Quote: ... 4) tagmentation was performed with 2ng input and sequencing library generated using the Nextera XT library prep kit (Illumina, #FC-131-1024). In short ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were sequenced using a 150 bp paired-end NextSeq High Output V2 reagent kit (Illumina, FC-404-2004, San Diego, CA). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... PolyA+ selected libraries were prepared with the TruSeq mRNA Stranded Library Prep Kit with TruSeq UDI96 indexed adaptors (Illumina, San Diego, CA). Samples were multiplexed and sequenced on the Illumina NovaSeq 6000 S4 to produce approximately 100 million ...
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bioRxiv - Genetics 2022Quote: ... to approximately 450 bp inserts followed by library preparation using the Seqlab TruSeq Nano DNA HT kit (20000903, Illumina, San Diego, USA). Library preparation was performed according to manufacturer’s instructions which included end repair ...
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bioRxiv - Genetics 2022Quote: ... Approximately 1 µg of total RNA were used to prepare the small RNA library according to the protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego). Then the single-end sequencing 50bp was performed on an Illumina Hiseq 2500 at LC Sciences (Hangzhou ...
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bioRxiv - Cancer Biology 2022Quote: ... using MiSeq V2 300 cycle reagent kit after the amplified library was purified with AMPure XP beads (Beckman Coulter, CA, USA) (Illumina, CA, USA) (10).
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bioRxiv - Bioengineering 2022Quote: ... the organoid was then resuspended in a direct permeabilization/tagmentation mastermix (25μl TD buffer (from Illumina Nextera XT DNA Library Prep Kit), 2.5μl Tn5 ...
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bioRxiv - Cancer Biology 2022Quote: ... The libraries were prepared from total RNA by using the TruSeq Stranded mRNA Library Prep kit according to the manufacturer’s instructions (Illumina, San Diego, CA). The libraries were analyzed for insert size distribution by using a 2100 BioAnalyzer High Sensitivity kit (Agilent) ...
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bioRxiv - Genomics 2022Quote: ... The construction of libraries was performed with Illumina preparation kits (Table S1) following the manufacturer’s recommendations (Illumina Inc., San Diego, CA, USA). The Nextera DNA Prep kit and Illumina DNA Prep were used for WGS ...
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bioRxiv - Plant Biology 2022Quote: ... RNA-seq libraries were constructed by the POPS platform (IPS2) using the TruSeq Stranded mRNA library prep kit (Illumina®, California, U.S.A.) according to the supplier’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... DNA libraries were prepared according to the manufacturer’s protocol using the Nextera DNA Flex Library Preparation Kit (Illumina, San Diego, CA, USA). Library preparation and bead clean-up was undertaken with the Mantis® Liquid Handler (Formulatrix ...
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bioRxiv - Microbiology 2022Quote: ... The same amount of the seven purified 3-cycle PCR products were further amplified using Platinum SuperFi II and the index primers from Nextera XT Index Kit v2 (Illumina cat. 15052163). The PCR products were purified using 1.8× beads ...
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bioRxiv - Genomics 2022Quote: Genomic libraries were prepared from 1 ng of high-quality genomic DNA using the Nextera DNA Flex Library Prep kit (now called Illumina DNA Prep, Illumina C.A., USA), following the manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... Amplified (1 ng) cDNA was used to generate barcoded libraries using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA). The size of the libraries was checked using the Agilent 2100 Bioanalyzer High Sensitivity DNA chip and their concentration was determined using the Qubit® fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: We prepared cDNA sequencing libraries for the transcriptome samples using the TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA, USA). Paired-end fragment reads were generated on an Illumina NextSeq sequencer using TG NextSeq® 500/550 High Output Kit v2 (300 cycles) ...
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bioRxiv - Synthetic Biology 2022Quote: ... pH 8.5 and sequenced on an Illumina NextSeq 550 following the manufacturer’s instructions using a NextSeq 500/550 Mid or High Output Kit (Illumina, 20024904 or 20024907), or on an Illumina NextSeq 1000 following the manufacturer’s instructions using NextSeq P2 v3 kits (Illumina ...
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bioRxiv - Plant Biology 2022Quote: ... Five micrograms of total RNA from each sample were used to construct an RNA-Seq library by using the TruSeq RNA Sample Preparation Kit according to the manufacturer’s instructions (Illumina, San Diego, CA). A total of 72 libraries (Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 000 nuclei were subjected to Tn5 transposition reaction using 2.5 μl TDE1 from Nextera DNA Library Prep Kit (Illumina, #FC-121-1030). After adding the transposition reaction mix ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNAs from WT and e2fabc at 9 DAG were used for construction of cDNA libraries using the TruSeq RNA Library Preparation Kit v2 (Illumina, United States) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... we generated a combination of noisy ONT sequencing (MinION R.9.4.1 flow cells with the LSK-109 ligation library prep kit) and Illumina sequencing (PE 150 bp, ∼49x coverage; Illumina NovaSeq 6000). Based on published ONT benchmarking (https://nanoporetech.com/accuracy) ...
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bioRxiv - Biochemistry 2022Quote: The sequencing libraries were built by tagmentation using 50 ng of ds cDNA with Illumina Nextera XT Kit (Illumina, #FC-131-1024) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... Paired end sequencing was performed on an Illumina NextSeq 500 with a 150-cycle high output kits (Illumina, Cat# FC-404-2002).