Labshake search
Citations for Illumina :
8001 - 8050 of 8781 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Resultant genomic DNA was treated with RNase A and prepared for sequencing using the Nextera XT kit (Illumina Corp., San Diego, CA). Libraries were uniquely dual-indexed and pooled ...
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bioRxiv - Genomics 2023Quote: ... RNA libraries were created and individually barcoded from 2.7 µg of template total RNA utilizing the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) as described in the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the KAPA RNA HyperPrep Kit and sequenced on an Illumina HiSeq® 4000 sequencer (Illumina, San Diego, CA) at a minimum of 25 million paired end reads (75bp ...
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bioRxiv - Microbiology 2023Quote: ... The Seqwell plexWell LP384 Library Preparation Kit and Native Barcoding Kit 24 V14 (SQK-NBD112.24) were used for barcode-indexed whole genome sequencing with Illumina NovaSeq system (Illumina San Diego, USA) and Oxford Nanopore MinIoN Mk1C device (Oxford Nanopore Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Complementary DNA libraries from total RNA samples were prepared with Illumina TruSeq RNA preparation kits according to the manufacturer’s instructions (Illumina, San Diego, CA). RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Santa Clara ...
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bioRxiv - Cancer Biology 2023Quote: ... The libraries were sequenced with 54 paired-end reads on a NovaSeq-6000 using a Reagent Kit v1.5 (Illumina Inc., San Diego). For CD45+ RNA-seq and the cell line RNA-seq ...
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bioRxiv - Microbiology 2023Quote: ... A second 4 nM pool was generated and sequenced on the Illumina NovaSeq 6000 with the NovaSeq 6000 S4 Reagent v1.5 (300 cycles) kit (20028312, Illumina, San Diego, CA) at the Molecular Biology Core Facilities at the Dana-Farber Cancer Institute (Boston ...
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bioRxiv - Immunology 2023Quote: ... 150 picograms of cDNA was used to make sequencing libraries by Nextera XT DNA Sample Preparation Kit (Illumina catalog#: FC-131-1024). Libraries were quantified with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific catalog# ...
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bioRxiv - Molecular Biology 2024Quote: ... Small RNA libraries were generated using NEXTFlex Small RNA Library Prep Kit v3 (Cat #NOVA-5132–06) and sequenced on the NextSeq 500 system (Illumina, CA, USA) by the Genomic Unit at Genyo ...
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bioRxiv - Microbiology 2024Quote: ... The library preparation and ribosome depletion were carried out using the Illumina Stranded Total RNA prep Ligation with Ribo-Zero Plus kit according to the manufacturer’s protocol (Illumina, San Diego, USA). The libraries were sequenced on the Illumina NextSeq1000 platform (1×100 bp ...
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bioRxiv - Microbiology 2024Quote: ... and 1ng of the cDNA was employed as a template for the construction of the sequencing library using the NexteraXT kit from (Illumina, Cambridge, UK). Subsequently ...
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bioRxiv - Microbiology 2024Quote: Genomic libraries of isolates from the CV region outbreak were prepared using the Illumina DNA Prep kit (Illumina, San Diego, CA, USA) and sequenced on a Nextseq instrument generating 2x150bp paired-end sequencing reads following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit v2 according to the manufacturer’s protocol (Illumina, San Diego, CA, USA). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Next generation sequencing library preparations were constructed following the manufacturer’s protocol (NEBNext® Ultra™ DNA Library Prep Kit for Illumina®). For each sample ...
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bioRxiv - Microbiology 2024Quote: Indexed sequencing libraries were generated from 1 ng genomic DNA using Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA) following the protocol of the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... RNA libraries were generated from 200 ng RNA using the Illumina total RNA Prep Ligation with Ribo-Zero Plus kit (Illumina, United States). In brief ...
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bioRxiv - Developmental Biology 2024Quote: ... The DNA was amplified by PCR using the Nextera DNA sample preparation and indexing kits (Illumina, FC-121-1030, FC-121-1011) as follows ...
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bioRxiv - Genomics 2024Quote: ... Libraries with 5% PhiX spike-in were sequenced on an Illumina NextSeq 2000 sequencer with P3 200 cycles kits (Illumina cat. #20040560) as paired-end ...
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STAT5B SH2 variants disrupt mammary enhancers and the stability of genetic programs during pregnancybioRxiv - Genetics 2024Quote: ... Libraries for sequencing were prepared according to the manufacturer’s instructions with TruSeq Stranded Total RNA Library Prep Kit with Ribo- Zero Gold (Illumina, RS-122-2301) and paired-end sequencing was done with a NovaSeq 6000 instrument (Illumina).
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bioRxiv - Genomics 2024Quote: ... Total RNA-seq libraries were generated from 1 μg of DNase-treated RNA using the TruSeq Stranded total RNA library preparation kit (Illumina, Cat# 15031048) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... RNA extracted from these fruits was pooled to create a single sample for the construction of cDNA libraries using the TruSeq RNA Library Prep Kit v2 (Illumina, Inc., USA) (Illumina ...
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bioRxiv - Genomics 2024Quote: ... RNA extracted from these fruits was pooled to create a single sample for the construction of cDNA libraries using the TruSeq RNA Library Prep Kit v2 (Illumina, Inc., USA) (Illumina, Inc., USA). The Agilent 2100 Bioanalyzer (Agilent ...
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bioRxiv - Systems Biology 2024Quote: ... Sequencing library preparation was conducted per the guidelines provided in the MiSeq™ Reagent Kit Preparation Guide (Illumina, San Diego, CA, USA) as outlined by (Caporaso et al. ...
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bioRxiv - Plant Biology 2024Quote: ... RNA libraries for sequencing were prepared using TruSeq Stranded mRNA sample prep kit with 96 dual indexes (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions with the following changes ...
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bioRxiv - Microbiology 2024Quote: ... RiboZero: rRNA was removed from 10 ng of total RNA using Ribo-Zero(TM) rRNA Removal Kit (Illumina, San Diego, CA, USA). Stranded cDNA libraries were generated using the Illumina Truseq Stranded mRNA Library Prep kit ...
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bioRxiv - Evolutionary Biology 2024Quote: ... resuspended in 25 μL of 2x TD buffer and 2.5 μl of Tn5 transposase from the Nextera DNA Library Prep kit (Illumina, San Diego, CA), and incubated at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... purified and ligated to 3′ and 5′ RNA adapters derived from TruSeq Small RNA Library Preparation Kit (Illumina, San Diego, CA, USA). Small RNA libraries were created using TruSeq Small RNA Library Preparation Kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries for Δ2 and Δ3 lines were prepared with 1 μg of gDNA per sample using the TruSeq® DNA PCR-Free Low-Throughput Library Prep Kit (Illumina, 20015962) according to the manufacturer’s directions ...
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bioRxiv - Systems Biology 2024Quote: ... The cDNA were then fragmented and amplified for sequencing using the Illumina Nextera XT DNA Library Preparation kit (Illumina, FC-131-1024) using custom primers that enabled the specific amplification of only the 3’ ends ...
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bioRxiv - Immunology 2024Quote: ... The V(D)J libraries were then denatured and diluted using a MiSeq Reagent Kit v3 (150- cycle) (Illumina, MS-102-3001) and sequenced on an lllumina MiSeq ...
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bioRxiv - Physiology 2024Quote: ... high-sensitivity spectrofluorometric measurement was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat #20020595, Illumina, San Diego, CA). Each sample was fitted with one of 96 adapters containing a different 8 base molecular barcode for high level multiplexing ...
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bioRxiv - Molecular Biology 2024Quote: ... Paired-end sequencing (2×75 cycles) was performed on an Illumina NextSeq 550 sequencer using a NextSeq 500/550 High Output Kit v2.5 (Illumina, San Diego, CA).
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bioRxiv - Immunology 2024Quote: ... cDNA was diluted to an average of 200 pg/µl and 100 pg cDNA from each cell was tagmented by adding 1 µl TD and 0.5 µl ATM from a Nextera XT DNA Library Preparation Kit (Illumina: FC-131-1096) to 0.5 µl diluted cDNA in each well of a fresh 384-well plate ...
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bioRxiv - Immunology 2024Quote: ... and sequencing was performed in a 2x150 bp paired end format using a NovaSeq 6000 v1.5 100-cycle reagent kit (Illumina, Cat no. 20028316). Base calling was performed with Illumina Real Time Analysis (RTA ...
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bioRxiv - Genetics 2024Quote: Libraries were sequenced at between 40,000 and 50,000 fragment reads per cell following Illumina’s standard protocol using the Illumina cBot and HiSeq 3000/4000 PE Cluster Kit (Illumina, San Diego, CA). The flow cells were sequenced as 100 × 2 paired end reads on an Illumina HiSeq 4000 HD using HiSeq 3000 / 4000 sequencing kit and HCS v3.4.0.38 collection software ...
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bioRxiv - Plant Biology 2024Quote: ... A 2×150 bp paired-end sequencing library was prepared with the NovaSeq 6000 SP Reagent Kit v1.5 (Illumina, San Diego, USA) and sequenced in two lanes of a NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Genomics 2024Quote: ... and 1 μL of TAE polymerase (TruePrep® DNA Library Prep Kit V2 for Illumina®, Cat. no. TD501-02, Vazyme Biotech). PCR thermocycling was carried out in Biometra Tone 96G thermocyclers (Analytik Jena) ...
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bioRxiv - Biochemistry 2024Quote: ... RNA samples were sent to RiboBio (Guangzhou, China) company for cDNA library construction using NEBNext® Ultra™ RNA Library Prep Kit for Illumina (Illumina) and high-throughput sequencing on Illumina sequencing platform choosing PE150 mode to obtain sequence information from both ends.
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bioRxiv - Immunology 2024Quote: ... a 2nd PCR was performed to barcode the individual samples using Index primers (TruSeq HT Kits, Illumina Inc., San Diego, California, USA) able to bind the adapter-region established during the previous PCR.
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bioRxiv - Genomics 2024Quote: Short-read sequencing libraries on human stool samples were prepared using the Illumina DNA Prep Kit (Illumina, Inc. for research use only). Libraries were pooled in equal concentration and sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... and prepared as a library with 200 ng total RNA input using polyA enrichment with the TruSeq Stranded mRNA kit (Illumina, SanDiego, USA). The library was sequenced with a 2x 51 bp paired-end protocol with Illumina NovaSeq6000 (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... and 50% Phi-X spike-in and was pre-sequenced on a MiSeq Reagent Nano Kit v2 (MS-102-2001 Illumina, SD, USA) single-end 1×51 bp run ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were diluted to a final concentration of 4 nM and sequenced with the NextSeq 500/550 HighOutput Kit v2.5 (75 cycles, Illumina, cat. no. 20024906), with 75 bp reads and pair-end sequencing ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The resulting cDNA library was prepared for sequencing on an Illumina platform using the Nextera XT DNA Library Prep Kit (Illumina, CA, USA) following the manufacturer’s protocol with dual index primers ...
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bioRxiv - Cell Biology 2024Quote: Libraries for whole transcriptome RNA sequencing were prepared using the Stranded Total RNAseq with Ribo-zero Plus kit (Illumina, San Diego, CA) as per manufacturer’s instructions starting with an input of 50 ng of RNA and 14 cycles of final PCR amplification ...
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bioRxiv - Plant Biology 2020Quote: ... A second round of PCR was used to add the dual indices with the Nextra XT Index Kit (Illumina, San Diego, CA, USA). The quality of these sequencing libraries was determined by the Qubit® 2.0 Fluorometer ...
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bioRxiv - Developmental Biology 2021Quote: ... One microgram of total RNA was used to create each sequencing library using a Truseq RNA Sample Preparation Kit (Illumina, RS-122-2001). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... ATAC-seq libraries for murine endothelial cells were processed as previously described (Buenrostro et al., 2015) and libraries were generated using the Nextera DNA Sample Preparation Kit (Illumina, FC-121-1030). The quality of purified DNA libraries was checked by Agilent High Sensitivity DNA kit (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 ng of total RNA from each sample was used for the RNA-seq library preparation using the TruSeq stranded mRNA kit (Illumina, San Diego, CA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... were prepared from HepG2 cell samples using 200–500 ng of total RNA following the manufacturer’s instructions for the TruSeq RNA kit (Illumina, San Diego, CA, USA). With this kit ...