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Citations for GE Life Sciences :
2501 - 2550 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and the supernatant injected (using 1 ml injection loop) in a Superdex 75 10/300GL gel filtration column (GE Healthcare), previously equilibrated with 50mM Tris pH8 buffer ...
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bioRxiv - Microbiology 2020Quote: ... A 500-µL sample was subjected to size exclusion chromatography on a Superose 12 10/300 GL column (GE Healthcare) in buffer P using an ÄKTA pure (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Cell suspension was passed through a 40 μm plastic cell strainer and overlaid onto 10 mL Ficoll-Hypaque (GE Healthcare), followed by centrifugation (1000x g ...
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bioRxiv - Microbiology 2020Quote: ... Buffer exchange to a storage/reaction buffer (50 mM Tris, 300 mM NaCl, pH 8.0) was performed with a desalting column (PD-10, GE Healthcare) and the protein was transferred to a conical tube and diluted to 1 mg/mL as determined by absorbance at 280 nm for long-term storage ...
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bioRxiv - Microbiology 2020Quote: ... the flow-through was buffer exchanged to PBS and concentrated using Vivaspin filters (10 kDa molecular weight cutoff; GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... Complex formation was assayed by comparing the elution volumes in SEC on either a Superdex 200 Increase 10/300 or Superdex 200 Increase 3.2/300 (GE Healthcare) column ...
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bioRxiv - Biochemistry 2021Quote: ... After Bio-beads removal the mixture was centrifuged and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) column equilibrated with buffer 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... agitated for 30 min at room temperature before the buffer was changed to PBS (pH 7.4) with a PD-10 desalting column (GE Healthcare). Full-length D-serFS was then concentrated to 200-300 μM using centricons (Vivaspin 500 ...
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bioRxiv - Neuroscience 2020Quote: Protein samples concentrated to ∼1.0 g/L were injected into an HPLC-driven SEC column (Superdex 200 10/30 column, GE Healthcare) equilibrated with HBS-C buffer ...
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bioRxiv - Neuroscience 2020Quote: ... labelled protein samples were subjected to size exclusion chromatography at 4°C (Superdex 200 Increase 10/300 GL, GE Healthcare) in 50 mM phosphate (pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of purified sample (3.8 mg/mL TbMORN1(7-15) and 2.6 mg/ml TgMORN1(7-15)) were injected onto a Superdex 200 10/300 (GE Healthcare) column and the flow rate was set to 0.5 ml/min ...
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bioRxiv - Biophysics 2021Quote: ... ∼900 μL of 100 μM TS-β1AR was applied at 0.3 mL/min to a 24-mL Superdex 200 Increase 10/300 GL column (GE healthcare) pre-equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µL of sample (10 µl injection loop loaded with 50 µL sample) were injected on a Superdex 75 PC 3.2/30 (GE Healthcare) analytical size exclusion column preequilibrated with the protein buffer ...
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bioRxiv - Immunology 2022Quote: ... pooled plasma (n=14-15 per pool) was injected onto a Superose 6 HR 10/300 GL column (GE Healthcare) and eluted at a constant flow rate of 500 μL/min ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a Superdex 200 10/300 GL column using an ÄKTA chromatography system (GE Healthcare Life Sciences). 0.5 ml or 1 ml fractions were collected and analyzed by Western blotting using antibodies against HOIP and V5.
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bioRxiv - Biochemistry 2022Quote: ... ΔCter and free p85α were further subjected to gel filtration using SuperdexTM 200 10/300 GL Increase from GE healthcare. After gel filtration ...
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bioRxiv - Biochemistry 2022Quote: ... and then immobilized in 7 cm IPG strips (pH 3-10 : 70×3×0.5 mm) and a linear gradient (NL) (GE Healthcare Immobiline™ Dry Strip IPG ...
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bioRxiv - Biochemistry 2022Quote: ... and washed with 10 column volumes washing buffer (20 mM Tris, 500 mM NaCl, pH 7.8) using an AKTA explorer 100 (GE Healthcare). ARF-DBD proteins were eluted by 1 h incubation with 40 mM DTT in washing buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Samples collected from IMAC were exchanged into size exclusion chromatography (SEC) buffer (20 mM Na2HPO4, 150 mM NaCl, pH 7.4) using either a HiPrep 26/10 desalting column (GE Healthcare) or through multiple rounds of concentration and dilution using an Amicon Ultra-15 filter unit with a 10 kDa molecular weight cut-off ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing tagged-SbtB7001 were pooled and transferred into binding buffer using a HiPrep 26/10 Desalting column (GE Healthcare). The His6–Ub tags were removed using a ubiquitin carboxyl-terminal hydrolase 2 catalytic core domain (USP2cc):SbtB7001 molar ratio of 1:25 at 4 °C overnight as previously described [14] ...
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bioRxiv - Microbiology 2019Quote: ... Eluates were buffer exchanged into gel filtration buffer (25 mM Tris-Cl, 150 mM NaCl, pH 7.5) via HiPrep 26/10 desalting column (GE Healthcare), followed by overnight incubation with Ulp-1 to cleave off the His6-tagged SUMO moiety ...
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bioRxiv - Biochemistry 2019Quote: ... Elution was performed with the lysis buffer containing 250 mM imidazol pH 8.0 and eluted proteins were directly attached on a 10 ml Streptavidin Sepharose (GE Healthcare). Proteins were extensively washed with Strep buffer (20 mM Hepes ...
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bioRxiv - Biophysics 2019Quote: ... Size exclusion chromatography was carried out using Superdex S75 16/60 HiLoad and Superdex 75 10/300GL columns (GE Healthcare) with 20 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... 5-10 µg of proteins were resolved on 4–12% SDS-polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with various antibodies prior to development with ECL prime (GE Healthcare) ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were separated into 24 fractions using the 24 cm IPG strips pH 3-10 NL (GE HealthCare, Chicago, IL). To achieve the required low salt concentration 2.5 mg wheat germ extract was diluted with water and centrifuged 10 min. ...
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bioRxiv - Biochemistry 2019Quote: ... The assembly and purity of complexes from peak fractions of the Superdex200 10/300 GL gel filtration column (GE Healthcare) was assessed by SDS-PAGE gel electrophoresis and SimplyBlue SafeStain (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... The most concentrated IR-ECD-containing fraction was either desalted using disposable 8.3 ml Sephadex G-25 PD-10 desalting columns (GE Healthcare) equilibrated with HBS by centrifugation ...
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bioRxiv - Biochemistry 2019Quote: ... the concentrated protein was gel-filtrated using a Superdex 200 10/300 GL column equilibrated in HBS at a flow rate of 0.5 ml/min and 4 °C (GE Healthcare). Peak fractions at a final OD280nm of ∼ 0.4 (∼ 1.4 μM ...
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bioRxiv - Biophysics 2019Quote: Dimer hydrodynamic radii were compared by size-exclusion chromatography (SEC) using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated with 1 M NaCl 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: Solid-phase synthesis of oligoribonucleotide was performed on a 1 μmol scale using AKTA Oligopilot plus 10 synthesizer (GE Healthcare) and Custom PrimerSupport™ Ribo A 40 (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... Base subcomplex was further purified by size-exclusion chromatography using a Superose™ 6 increase 10/300 column (GE Healthcare) pre-equilibrated with base GF buffer (60 mM HEPES pH 7.6 ...
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bioRxiv - Molecular Biology 2019Quote: ... FANCD2 and FANCI at 1 µM were sequentially run on a Superdex 200 10/300 column (GE Healthcare Life Sciences) equilibrated in 50 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Eluted proteins were further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Life Sciences). Purified proteins were concentrated by ultrafiltration prior to flash freezing with liquid nitrogen.
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bioRxiv - Biophysics 2020Quote: ... A volume of 50 μl of each sample at 3 mg·ml−1 was loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 25 mM Hepes pH 7.8 ...
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bioRxiv - Biophysics 2019Quote: ... diluted such that the NaCl concentration was < 50 mM and applied to a Mono Q 10/100 GL (GE Healthcare) equilibrated in 100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was filtered and added to a pre-equilibrated (50 mM Tris, pH 7.8, 10% glycerol) Nickel Sepharose Fast Flow column (GE Healthcare). Proteins were eluted with 250 mM imidazole and dialysed into 50 mM Tris pH 7.8 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The unreacted DBCO molecules were eliminated by a Sephadex G-25 in PD-10 Desalting Columns (GE Healthcare Life Sciences). To make fluorescently labeled DBCO modified FBS proteins or individual proteins (BSA or APO H) ...
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bioRxiv - Biochemistry 2020Quote: ... The measurements were done at flowrate of 0.5 ml/min over an S-75 Superdex 10/300 column (GE Healthcare) in 20 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... and flow-through containing the cleaved SALM3-LRR was collected and gel filtered with Superdex 75 10/300 (GE Healthcare) in 60 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: The state of XynA in solution was analyzed by a Superdex 200 (10/300) increase column (GE Healthcare, United States). First ...
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bioRxiv - Biochemistry 2020Quote: Analytical size exclusion chromatography was performed with 20μl protein samples loaded on a Superdex 200 Increase 10/300 GL column (GE Healthcare) mounted on an Agilent 1260 HPLC ...
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bioRxiv - Biochemistry 2020Quote: ... DTT or diamide was removed by gel filtration on a Superdex 200 10/300 Increase column (GE Healthcare, Chicago, USA). For isotopically-labeled samples used in nuclear magnetic resonance spectroscopy (NMR ...
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bioRxiv - Molecular Biology 2021Quote: ... A 50 µg sample of HELB was loaded at 0.5 mL/min onto a Superose 6 10/300 size-exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 (DE3) carrying the plasmid pPyoS5 with initial purification using a cation exchange column (CM16/10 FF, GE Healthcare; buffers ...
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bioRxiv - Microbiology 2020Quote: ... inoculum was removed and cells were overlaid with DMEM + 10% fetal bovine serum (GE Healthcare Life Science, Piscataway, NJ, USA). After 1 day ...
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bioRxiv - Synthetic Biology 2020Quote: ... Pure proteins with the correct homooligomeric conformation were collected from a Superose 6 10/300 GL SEC column (GE Healthcare) in Tris-buffer (TBS ...
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bioRxiv - Biophysics 2020Quote: ... The solution was then spin-filtered through a 0.2 µm filter for injection onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (GE Healthcare) for standard gel filtration analysis with a 0.5 mL/min flow rate ...
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bioRxiv - Immunology 2020Quote: ... 7.5×104 congenically distinct cells of each sorted memory population were adoptively transferred and tumor-bearing mice were subsequently immunized with 10 μg of GP33-41 and 2 μg of poly(I:C) (GE Healthcare) s.c ...
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bioRxiv - Plant Biology 2019Quote: ... The suspension was centrifuged for 15 minutes at 4°C and 15,000 g to remove cell debris and the clear supernatant was applied to a PD-10 desalting column (GE healthcare) to remove excess free biotin using the gravity protocol according to the manufacturer’s instructions ...